• Title/Summary/Keyword: NTF2

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Screening Molecular Chaperones Similar to Small Heat Shock Proteins in Schizosaccharomyces pombe

  • Han, Jiyoung;Kim, Kanghwa;Lee, Songmi
    • Mycobiology
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    • v.43 no.3
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    • pp.272-279
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    • 2015
  • To screen molecular chaperones similar to small heat shock proteins (sHsps), but without ${\alpha}$-crystalline domain, heat-stable proteins from Schizosaccharomyces pombe were analyzed by 2-dimensional electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Sixteen proteins were identified, and four recombinant proteins, including cofilin, NTF2, pyridoxin biosynthesis protein (Snz1) and Wos2 that has an ${\alpha}$-crystalline domain, were purified. Among these proteins, only Snz1 showed the anti-aggregation activity against thermal denaturation of citrate synthase. However, pre-heating of NTF2 and Wos2 at $70^{\circ}C$ for 30 min, efficiently prevented thermal aggregation of citrate synthase. These results indicate that Snz1 and NTF2 possess molecular chaperone activity similar to sHsps, even though there is no ${\alpha}$-crystalline domain in their sequences.

3D Micromorphology Producing within Poly(lactic acid) Skeleton Using Room-Temperature Ionic Liquids: From Particulate, Fibrous or Porous Scaffolds to Beads

  • Shin, Ueon-Sang;Kim, Jong-Gyu
    • Bulletin of the Korean Chemical Society
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    • v.33 no.7
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    • pp.2295-2298
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    • 2012
  • We describe herein a three-dimensionally diverse micropatterning of poly(lactic acid), as a biopolymer, using 1-butyl-3-methylimidazolium-based room-temperature ionic liquids (bmim-based RTILs), [bmim]X (X = $SbF_6$, $PF_6$, $NTf_2$, Cl). Utilizing the hydrophobic bmim-based RTILs, [bmim]X (X = $SbF_6$, $PF_6$, $NTf_2$) and a phase separation technique, we were able to produce white and opaque membranes with a three-dimensional structure closely packed with particles ($10-50{\mu}m$ in diameter). The particlulate structure, made by the assistance of [bmim]$NTf_2$ and DCM, interestingly transformed to a fibrous structure by using a cosolvent, e.g., DCM/$CF_3CH_2OH$. When we used an increased amount of [bmim]$NTf_2$, the particles were effectively detached and macrosized ($100-500{\mu}m$ in diameter) and the oval-shaped beads were obtained in a powder form. By varying the counter-anion type of the imidazolium-based RTIL, for example from $NTf_2^-$ to $Cl^-$, the particulate 3D-morphology was once more transformed to a porous structure. These reserch results could be potentially useful, as a method to fabricate particulate scaffolds, fibrous or porous scaffolds, and beads as a biopolymer device in diverse fields including drug delivery, tissue regeneration, and biomedical engineering.

NEW TECHNIQUE IN THE USE OF VIBRO-ACOUSTICAL RECIPROCITY WITH APPLICATION TO THE NOISE TRANSFER FUNCTION MEASUREMENT

  • Ko, K.H.;Kook, H.S.;Heo, S.J.
    • International Journal of Automotive Technology
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    • v.7 no.2
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    • pp.173-177
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    • 2006
  • A noise transfer function(NTF) is the frequency response function between an input force applied to an exterior point of a vehicle body and the resultant interior sound pressure usually measured at the driver's ear position. It represents the measure of noise sensitivity for the output force transmitted to the joints between the body and chassis. The principle of vibro-acoustic reciprocity is often utilized in the measurement of NTF. One difficulty in using the volume source is that most of the previously proposed methods require the knowledge of the volume velocity of the acoustic source in advance. A new method proposed in the present work does not require any calculation related with the volume velocity of the acoustic source, but still yields even more accurate results both in the amplitude and phase of the NTF. In the present work, the new method is applied to obtain NTF data for a midsize sedan.

Wind Turbine Power Performance Testing using Nacelle Transfer Function (나셀 변환 함수를 이용한 풍력터빈 출력성능평가)

  • Kim, Hyeon-Wu;Ko, Kyung-Nam;Huh, Jong-Chul
    • Journal of the Korean Solar Energy Society
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    • v.33 no.4
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    • pp.51-58
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    • 2013
  • A study on power performance testing of a wind turbine which has no met-mast at a distance of 2~4 rotor diameter was carried out using the Nacelle Transfer Function, NTF, according to IEC 61400-12-2. The wind data for this study was measured at HanKyoung wind farm of Jeju Island. The NTF was modeled using the correlation between wind speeds from the met-mast and from the wind turbine nacelle within 2~4 rotor diameter from the met-mast. The NTF was verified by the comparison of estimated Annual Energy Productions, AEPs, and binned power curves. The Nacelle Power Curve, NPC, was derived from the nacelle wind speed data corrected by NTF. The NPC of wind turbine under test and the power curve offered by the turbine manufacturer were compared to check whether the wind turbine is properly generating electricity. Overall the NPC was in good agreement with the manufacturer's power curve. The result showed power performance testing for a wind turbine which has no met-mast at a distance of 2~4 rotor diameter was successfully carried out in compliance with IEC 61400-12-2.

Acridine Fluorescence Behaviors in Different Polymeric Microenvironments Directed by C2-Proton-Acidity of Imidazolium-Based Ionic Liquids

  • Ji, Myoung-Jin;Kim, Jong-Gyu;Shin, Ueon-Sang
    • Bulletin of the Korean Chemical Society
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    • v.33 no.8
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    • pp.2489-2493
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    • 2012
  • A new fluorescent system (acridine/RTIL hybrid gel) confined in the 3D micro-structure of a poly(lactic acid) membrane were prepared from 1-butyl-3-methylimidazolium-based ionic liquids ([bmim]X (X = $SbF_6$, $NTf_2$, Cl); RTILs), poly(lactic acid) (PLA), and acridine via the sol-gel route. SEM images showed that, in the presence of [bmim]$SbF_6$ and [bmim]$NTf_2$, 3D-ly paticulated structures were created inside the PLA membranes and acridine/RTIL hybrid gels were confined in gabs of particulates. However, the use of [bmim]Cl induced the formation of a 3D-ly porous structure containing the hybrid gel of acridine/[bmimCl in the micropores. The three fluorescent systems exhibited different fluorescence behaviors (fluorescence maximum and intensity) depending on the C2-H acidity scale of the RTILs (or their anion type). Acridine gels hybridized with [bmim]$SbF_6$ and [bmim]$NTf_2$ showed blue fluorescence with relative high intensity, whereas the hybrid gel with [bmim]Cl exhibited almost no fluorescence under dry conditions. However, the acridine/[bmim]Cl hybrid system in the micro-porous PLA membrane started to emit fluorescent light under humid conditions and showed a possible response, indicating that it could be applied as a humidity sensor.

Derivation of Nacelle Transfer Function Using LiDAR Measurement (라이다(LiDAR) 측정을 이용한 나셀전달함수의 유도)

  • Kim, Hyun-Goo;Kang, Yong-Heack;Yun, Chang-Yeol
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.9
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    • pp.929-936
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    • 2015
  • Nacelle anemometers are mounted on wind-turbine nacelles behind blade roots to measure the free-stream wind speed projected onto the wind turbine for control purposes. However, nacelle anemometers measure the transformed wind speed that is due to the wake effect caused by the blades' rotation and the nacelle geometry, etc. In this paper, we derive the Nacelle Transfer Function (NTF) to calibrate the nacelle wind speed to the free-stream wind speed, as required to carry out the performance test of wind turbines according to the IEC 61400-12-2 Wind-Turbine Standard. For the reference free-stream wind data, we use the Light Detection And Ranging (LiDAR) measurement at the Shinan wind power plant located on the Bigeumdo Island shoreline. To improve the simple linear regression NTF, we derive the multiple nonlinear regression NTF. The standard error of the wind speed was found to have decreased by a factor of 9.4, whereas the mean of the power-output residual distribution decreased by 6.5 when the 2-parameter NTF was used instead of the 1-parameter NTF.

Usefulness assessment of the Volumetric Modulated Arc Therapy technique for reducing low-dose areas during radiotherapy for patients with multiple metastatic cancers (다발성 전이암 환자의 방사선치료 시 저선량 영역 감소를 위한 용적조절 회전 방사선치료(Volumetric Modulated Arc Therapy) 기법의 유용성 평가)

  • Yun-won Choi;Dong-min Jeong;Se-young Kim;Ryeong-hwang Park;I-ji Kim;Yong-wan Cho;Yongjae Kwon;Byeol-nim Park;Gyeong-min Yoo;Ho-kyung Moon;Dong-jae Jang;Jae-young Lee;Dayoung Lim;Sang-gyu Lee;Jong-geol Baek
    • The Journal of Korean Society for Radiation Therapy
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    • v.35
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    • pp.23-31
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    • 2023
  • Purpose: The purpose of this study is to evaluate the usefulness of Non-Treat Functionality Volumetric Modulated Arc Therapy(NTF-VMAT) and Treat Functionality VMAT(TF-VMAT) treatment plans in reducing the low-dose area during radiation therapy for patients with multiple metastatic cancers. Materials and Methods: The study was conducted on an Arccheck phantom, treatment planning target locations were set in pairs at intervals of 2 cm, 4 cm, and 6 cm on the X, Y, and Z axes. Based on these location settings, the volume of the low-dose area in NTF-VMAT and TF-VMAT was measured and compared. Results: The results of the study showed that, within a prescription dose range of 10% ~ 70%, the difference in low-dose area volumes across each axis was as follows: On the X-axis, there was a maximum difference of -47.6% and a minimum difference of -2.2%. On the Y-axis, there was a maximum difference of -17.5% and a minimum difference of -7.3%. The Z-axis showed a maximum difference of -39.7%, with the smallest difference being -6.8%. Conclusion: In radiation therapy for patients with multiple metastatic cancers, the TF-VMAT treatment plan was able to reduce the low-dose area by 10-40% compared to NTF-VMAT. This suggests that utilizing Treat Functionality, which includes the Island block technique, improves dose distribution and minimizes side effects, making it beneficial for the treatment of patients with multiple metastatic cancers.

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Extraction behaviors of platinum group metals in simulated high-level liquid waste by a hydrophobic ionic liquid bearing an amino moiety

  • Wu, Hao;Kim, Seong-Yun;Takahashi, Tadayuki;Oosugi, Haruka;Ito, Tatsuya;Kanie, Kiyoshi
    • Nuclear Engineering and Technology
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    • v.53 no.4
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    • pp.1218-1223
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    • 2021
  • A hydrophobic ionic liquid including an amino moiety ([DiOcAPmim][NTf2]) was synthesized. Its extraction behaviors towards Pd(II), Ru(III), Rh(III) were investigated in nitric acid aqueous solution as a function of contact time, effect of concentration of nitric acid, effect of temperature, and effect of co-existing metal ions. The extraction kinetics of Pd(II) was fairly fast and extraction equilibrium can be attained within only 5 min under the [HNO3] = 2.05 M. When [HNO3]< 1 M, the extraction percentage of Pd(II), Ru(III), Rh(III) were all above 80%. When [HNO3] reached 2 M, all of the extraction percentage decreased and in an order of Pd(II)>Ru(III)>Rh(III). When [HNO3]> 2 M, the extraction performance gradually recovered. The effect of temperature can slightly affect the extraction performance of Pd(II). Furthermore, in simulated high-level liquid waste, [DiOcAPmim][NTf2] showed a better preference towards Pd(II) under the interference of various other co-existing metal ions.

Expressional Profiling of Molecules Associated with Epigenetic Methylation-Related Fertility in the Rat Testis during Postnatal Period

  • Seo, Hee-Jung;Lee, Seong-Kyu;Baik, Haing-Woon;Lee, Ki-Ho
    • Journal of Animal Science and Technology
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    • v.54 no.3
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    • pp.157-163
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    • 2012
  • The male reproduction is precisely controlled by a number of intrinsic and extrinsic factors. These factors usually involve in expressional regulation of various molecules influencing on sperm production in the testis. A number of ways are employed to control the transcription of specific genes, including epigenetic modifications of DNA and histone molecules. DNA methylation of CpG dinucleotides is a commonly used regulatory mechanism for testicular genes associated with the fertility. Previous studies have demonstrated the infertility induced by improper DNA methylation of these genes. In the present research, we attempted to determine transcriptional expression of some of these genes in the rat testis at different postnatal ages using real-time PCR analysis. These genes include neurotrophin 3 (Ntf3), insulin-like growth factor II (Igf2), JmjC-domain-containing histone demethylase 2A 1 (Jhm2da), paired box 8 transcription factor (Pax8), small nuclear ribonucleoprotein polypeptide N (Snrpn), and 5,10-methylenetetrahydrofolate reductase (Mthfr). The expression levels of Ntf3, Igf2, and Snrpn genes were the highest at the neonatal age, followed by transient decreases at the prepubertal age. Expression of Jhm2da and Mthfr genes were continuously increased from the neonate to 1 year of age. The levels of Pax8 mRNA at the early ages were higher than those at the later ages of postnatal development. These findings suggest that expression of some fertility-associated testicular genes in the rat during postnatal period could be differentially regulated by the control of the degree of DNA methylation.

Esterification of Free Fatty Acids by Strong Acidic Ionic Liquids (강산성 이온성 액체에 의한 유리지방산의 에스테르화 연구)

  • Kim, Young-Joo;Lee, Jin-Suk;Kim, Deog-Keun;Rhee, Young-Woo;Han, Jeong-Sik
    • Korean Chemical Engineering Research
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    • v.45 no.3
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    • pp.286-290
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    • 2007
  • The esterification efficiency of several ionic liquids has been investigated to determine the feasibility for the conversion of free fatty acids to alkylester. Five ionic liquid catalysts having strong acidity, BPC[$AlCl_3$], BMIM[$Bf_4$], BMIM[$Pf_6$], EMIM[$Ntf_2$], BMIM[Otf], have been employed in this work. BPC[$AlCl_3$] has the highest esterification efficiency among the ionic liquid catalysts. Over 90% conversion efficiency has been achieved in the esterification of the simulated used cooking oil by BPC[$AlCl_3$] with two hours reaction time. Since BPC[$AlCl_3$] has several advantages such as high esterification activity, ease of separation from reaction mixture and reusability after treatment procedure, it will be a promising catalyst for the conversion of free fatty acids to esters in waste fats.