• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.104-110
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• 2011

Actinomycetes, Gram positive soil bacteria, are valuable microorganisms which produce useful secondary metabolites including antibiotics, antiparasitic substances, anti-cancer drugs, and immunosuppressants. Although a major family of actinomycetes, known as streptomycetes, has been intensively investigated at the molecular level for several decades, a potentially valuable and only recently isolated non-streptomycetes rare actinomycetes (NSRA) family has been poorly characterized due to lack of proper genetic manipulation systems. Here we report that a PCR-based genome screening strategy was performed with approximately 180 independently isolated actinomycetes strains to isolate potentially valuable NSRA strains. Thanks to this simple PCR-based genome screening strategy we were able to identify only seven NSRA strains, followed by 16S rRNA sequencing for confirmation. Through further bioassays, one potentially valuable NSRA strain (tentatively named Nocardiopsis species MMBL010) was identified which possessed both antifungal and antibacterial activities, along with the presence of polyketide synthase and non-ribosomal peptide synthase genes. Moreover, Nocardiopsis species MMBL010, which was intrinsically recalcitrant to genetic manipulation, was successfully transformed via E. coli-driven conjugation. These results suggest that PCR-based genome screening, followed by the establishment of an E. coli-driven conjugation system, is an efficient strategy to maximize potentially valuable compounds and their biosynthetic genes from NSRA strains isolated from various environments.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2014.06a
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• pp.8-10
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• 2014

정부의 북극항로 시험운항이 시행된 2013년 NSR횡단 선박 척수는 전년도에 비해 54%가 늘어난 71척에 이르렀으나 실제 화물수송량은 당초 예상량인 150만톤에도 못 미치는 1,355,897톤으로 전년도에 비해 7.5% 증가에 그치고 말았다. 이는 2013년 하절기 북극해의 얼음이 2012년도에 비해 훨씬 적게 녹았기 때문으로 분석되고 있다. 그러나 지구온난화로 인한 전체적인 해빙 크기의 감소추세는 큰 틀에서 벗어나지 않고 있으므로 NSR의 선점을 위해서는 화물의 경제성 평가와 더불어 고효율선의 운항에 대비할 필요가 있다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2014.10a
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• pp.154-156
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• 2014

지구온난화로 2000년대 초부터 급격하게 감소하던 북극의 해빙이 2012년 이후로 다소 주춤한 상태로 돌아서고 있다. 그러나 최근 북극항로를 이용하는 선박의 척수와 화물량은 꾸준히 증가하고 있어 이 항로에 대한 세계적인 관심이 집중되고 있다. 중세의 문명의 교류가 오아시스 루트와 초원 루트 그리고 말라카 해협을 통한 해상실크로드를 통해 이루어진 것처럼, 장차 아시아와 유럽을 잇는 북극항로가 신해양실크로드가 될 것인지 북극을 둘러싼 여러 가지 상황을 살펴보고자 한다.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2004.11a
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• pp.76-77
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• 2004

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2004.11a
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• pp.78-80
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• 2004

• Journal of Radiation Industry
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• v.5 no.3
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• pp.191-196
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• 2011

The economic scale of nuclear application is a good indicator to show how the radiation technology is useful and contribute to improve public welfare and living standard. Recent research in Japan shows that the economic scale of nuclear field was 4,112 B¥ for radiation application(46%) and 4,741 B¥ for nuclear energy (54%) playing a role of "two wheels of one cart" in nuclear field and the total 8,853 B¥ constitutes 1.8% of gross domestic products (GDP). The radiation application consisted of 2,295 B¥ (56%) in industry (semiconductor, sterilization, nondestructive testing, radiation processing of tires, etc.), 1,538 B¥ (37%) in medicine (therapy and diagnosis such as X-ray, nuclear medicine, computed tomography, etc.) and 279 B¥ (7%) in agriculture (mutation breeding, food irradiation, sterile insect technique, etc.). Radiation application by ${\gamma}$-ray, electron beam and ion beam is steadily increasing in Japan.