• Korean Journal of Environmental Biology
• /
• v.22 no.3
• /
• pp.472-477
• /
• 2004

To study the radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression was analyzed by DNA micro array. We constructed DNA chips including two thousand randomly digested genome spots of B. lentimorbus WJ5 and compared its quantitative aspect with seven mutants induced by gamma radiation $(^{60}/Co)$. From the cluster analysis of gene expression pattern, totally 408 genes were expressed and 27 genes were significantly upregulated by the gamma radiation in all mutants. Especially, genes involved in repair (mutL, mutM), energy metabolism (acsA, sdhB, pgk, yhjB, citB), protease (npr), and reduction response to oxidative stress (HMM) were simultaneously upregulated. It seems that the induction of the direct and/or indirect repair related genes in mutants induced by gamma radiation could be remarkably different from the adaptive responses against acute exposure to radiation.

• Journal of Korean Society for Atmospheric Environment
• /
• v.24 no.1
• /
• pp.30-42
• /
• 2008

In this study, the chemical compositions of $PM_{2.5}$ samples collected at the Redwood National Park IMPROVE site in California from March 1988 to May 2004 were analyzed to provide source identification and apportionment. A total of 1,640 samples were collected and 33 chemical species were analyzed by particle induced X-ray emission, proton elastic scattering analysis, photon induced X-ray fluorescence, ion chromatography, and thermal optical reflectance methods. Positive matrix factorization (PMF) was used to develop source profiles and to estimate their mass contributions. The PMF modeling identified five sources and the average mass was apportioned to motor vehicle (35.8%, $1.58\;{\mu}g/m^3$), aged sea salt (23.2%, $1.02\;{\mu}g/m^3$), fresh sea salt (21.4%, $0.94\;{\mu}g/m^3$), wood/field burning (16.1%, $0.71\;{\mu}g/m^3$), and airborne soil (3.5%, $0.15\;{\mu}g/m^3$), respectively. To analyze local source impacts from various wind directions, the CPF and NPR analyses were performed using source contribution results with the wind direction values measured at the site. These results suggested that sources of $PM_{2.5}$ are also sources of visibility degradation and then source apportionment studies derived for $PM_{2.5}$ are also used for understanding visibility problem.

• Proceeding of EDISON Challenge
• /
• 2016.03a
• /
• pp.228-233
• /
• 2016

Auxetic material is a material which has negative Poisson's ratio(NPR). Auxetic material shows some distinctive property like high energy absorbing property and high shear modulus. Among these, synclastic curvature is very interesting characteristic. When synclastic-curvature-material bends, it changes its shape like dome, contrary to non-auxetic material which changes its shape like saddle(anticlastic). This distinctive property could make it easy to manufacture curved structure like nose cone or wing panel in aerospace engineering. In this study, we studied a quantitative analysis about synclastic curvature of re-entrant panel with finite element model. We suggested a concept 'Degree of Synclasticity(DOS)', which means a ratio of curvature of load-direction and load-orthogonal direction. We studied the variation of DOS with two factor, unit cell inner angle(${\theta}$) and load position angle(${\phi}$). DOS decreases as ${\theta}$ increases because the unit cell goes out of auxetic-shape. As ${\phi}$ varies, DOS changes in a large range. So proper optimization of ${\phi}$ would be needed for application.

• Advances in aircraft and spacecraft science
• /
• v.7 no.3
• /
• pp.203-214
• /
• 2020

In many applications like the aircraft or the rockets/missiles, the flow from a nozzle needs to be expanded suddenly in an enlarged duct of larger diameter. The enlarged duct is provided after the nozzle to maximize the thrust created by the flow from the nozzle. When the fluid is suddenly expanded in an enlarged duct, the base pressure is generally lower than the atmospheric pressure, which results in base drag. The objective of this research work is to optimize the length to diameter (L/D) ratio of the enlarged duct using the CFD analysis in the flow field from the supersonic nozzle. The flow from the nozzle drained in an enlarged duct, the thrust, and the base pressure are studied. The Mach numbers for the study were 1.5, 2.0 and 2.5. The nozzle pressure ratios (NPR) of the study were 2, 5 and 8. The L/D ratios of the study were 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10. Based on the results, it is concluded that the L/D ratio should be increased to an optimum value to reattach the flow to an enlarged duct and to increase the thrust. The supersonic suddenly expanded flow field is wave dominant, and the results cannot be generalized. The optimized L/D ratios for various combinations of flow and geometrical parameters are given in the conclusion section.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.46 no.6
• /
• pp.1074-1087
• /
• 2022

This study reports an optimization of a 3D printed wrist brace (WB) for various tilting angles (0°, 45°, 90°) of the re-entrant (RE) pattern and thickness (2 mm, 4 mm) using thermoplastic polyurethane (TPU) filaments and thermoplastic elastomer (TPE) filaments. The actual printing time, weight, Poisson's ratio, and tensile property of the manufactured samples were analyzed. The results confirmed that the actual printing time and weight increased with increasing thickness, regardless of the filament type. All tilting angles of the WB showed a negative Poisson's ratio (NPR), the largest of which appeared at 90°. The results of the tensile property analysis showed that a 90° tilting angle also had the largest value of elongation and stress. From these results, we conclude that the most suitable wrist brace is one in which the actual printing time is low, the weight is minimized to a thickness of 2 mm, and the tilting angle of the RE pattern is 90° for good shock absorption. The choice of filaments may be decided upon according to the user's preference, since the TPU is stiff and the TPE is elastic.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.2
• /
• pp.194-203
• /
• 2009

$HpaG_{Xooc}$, from rice pathogenic bacterium Xanthomonas oryzae pv. oryzicola, is a member of the harpin group of proteins, eliciting hypersensitive cell death in non-host plants, inducing disease and insect resistance in plants, and enhancing plant growth. To express and secret the $HpaG_{Xooc}$ protein in Bacillus subtilis, we constructed a recombinant expression vector pM43HF with stronger promoter P43 and signal peptide element nprB. The SDS-PAGE and Western blot analysis demonstrated the expression of the protein $HpaG_{Xooc}$ in B. subtilis. The ELISA analysis determined the optimum condition for $HpaG_{Xooc}$ expression in B. subtilis WBHF. The biological function analysis indicated that the protein $HpaG_{Xooc}$ from B. subtilis WBHF elicits hypersensitive response(HR) and enhances the growth of tobacco. The results of RT-PCR analysis revealed that $HpaG_{Xooc}$ induces expression of the pathogenesis-related genes PR-1a and PR-1b in plant defense response.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.232-232
• /
• 2022

We reported in our recent studies that the combined treatment of Paenibacillus yonginensis DCY84^T (DCY84^T) and Silicon (Si) promotes initial plant growth and increases resistance to biotic and abiotic stress. To understand the molecular background of these phenotypes, Liquid Chromatography Mass Spectrometry (LC-MS) analysis was performed, and it was confirmed that unsaturated fatty acid metabolites such as oleic acid and linoleic acid decreased in response to the combined treatment of DCY84^T and Si. The stearoyl-acyl carrier protein desaturase (SACPD) introduces the cis double bond into the acyl-ACPs at C9, resulting in the production of unsaturated fatty acid. We identified OsSSI2 encoding SACPD in rice and found that the expression of OsSSI2 was reduced under DCY84^T and Si treatment. Furthermore, qRT-PCR analysis revealed that the expression of OsWRKY45, which is downstream of OsSSI2, was upregulated in response to DCY84^T and Si treatment. These results enable the speculation that activation of the salicylic acid (SA)-responsive gene, OsWRKY45, may contribute to enhancing biological stress resistance. Based on this, we propose a probable model for the rice defense pathway following DCY84^T and Si treatment. This model retains a WRKY45-dependent but NH1(NPR1)-independent SA signaling pathway.

• The Plant Pathology Journal
• /
• v.40 no.4
• /
• pp.346-357
• /
• 2024

This study was carried out to screen the antifungal activity against Colletotrichum acutatum, Colletotrichum dematium, and Colletotrichum coccodes. Bacterial isolate GP-P8 from pepper soil was found to be effective against the tested pathogens with an average inhibition rate of 70.7% in in vitro dual culture assays. 16S rRNA gene sequencing analysis result showed that the effective bacterial isolate as Bacillus siamensis. Biochemical characterization of GP-P8 was also performed. According to the results, protease and cellulose, siderophore production, phosphate solubilization, starch hydrolysis, and indole-3-acetic acid production were shown by the GP-P8. Using specific primers, genes involved in the production of antibiotics, such as iturin, fengycin, difficidin, bacilysin, bacillibactin, surfactin, macrolactin, and bacillaene were also detected in B. siamensis GP-P8. Identification and analysis of volatile organic compounds through solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) revealed that acetoin and 2,3-butanediol were produced by isolate GP-P8. In vivo tests showed that GP-P8 significantly reduced the anthracnose disease caused by C. acutatum, and enhanced the growth of pepper plant. Reverse transcription polymerase chain reaction analysis of pepper fruits revealed that GP-P8 treated pepper plants showed increased expression of immune genes such as CaPR1, CaPR4, CaNPR1, CaMAPK4, CaJA2, and CaERF53. These results strongly suggest that GP-P8 could be a promising biocontrol agent against pepper anthracnose disease and possibly a pepper plant growth-promoting agent.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.10
• /
• pp.1250-1258
• /
• 2009

Bacillus spp., as a type of plant growth-promoting rhizobacteria (PGPR), were studied with regards promoting plant growth and inducing plant systemic resistance. The results of greenhouse experiments with tobacco plants demonstrated that treatment with the Bacillus spp. significantly enhanced the plant height and fresh weight, while clearly lowering the disease severity rating of the tobacco mosaic virus (TMV) at 28 days post-inoculation (dpi). The TMV accumulation in the young non-inoculated leaves was remarkably lower for all the plants treated with the Bacillus spp. An RT-PCR analysis of the signaling regulatory genes Coil and NPR1, and defense genes PR-1a and PR-1b, in the tobacco treated with the Bacillus spp. revealed an association with enhancing the systemic resistance of tobacco to TMV. A further analysis of two expansin genes that regulate plant cell growth, NtEXP2 and NtEXP6, also verified a concomitant growth promotion in the roots and leaves of the tobacco responding to the Bacillus spp.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.23 no.3
• /
• pp.28-34
• /
• 2019

The thrust control calculation according to the operation of plug nozzle for cold air test and the analysis of the flow characteristics of the pintle shape and operation pressure are performed. The numerical computation was verified by comparing the flow structure and the coefficient of thrust with the experimental data. It was confirmed that the nozzle throat was formed at the design position on each pintle shape, and thrust control up to 1:8 was achieved only by the stroke change. Finally, although the aerospike nozzle is autonomous, it is unfavorable in the under_expansion condition, if it is designed for a very low nozzle pressure ratio.