• Molecules and Cells
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• v.42 no.1
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• pp.28-35
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• 2019

Animals need to be able to alter their developmental and behavioral programs in response to changing environmental conditions. This developmental and behavioral plasticity is mainly mediated by changes in gene expression. The knowledge of the mechanisms by which environmental signals are transduced and integrated to modulate changes in sensory gene expression is limited. Exposure to ascaroside pheromone has been reported to alter the expression of a subset of putative G protein-coupled chemosensory receptor genes in the ASI chemosensory neurons of C. elegans (Kim et al., 2009; Nolan et al., 2002; Peckol et al., 1999). Here we show that ascaroside pheromone reversibly represses expression of the str-3 chemoreceptor gene in the ASI neurons. Repression of str-3 expression can be initiated only at the L1 stage, but expression is restored upon removal of ascarosides at any developmental stage. Pheromone receptors including SRBC-64/66 and SRG-36/37 are required for str-3 repression. Moreover, pheromone-mediated str-3 repression is mediated by FLP-18 neuropeptide signaling via the NPR-1 neuropeptide receptor. These results suggest that environmental signals regulate chemosensory gene expression together with internal neuropeptide signals which, in turn, modulate behavior.

• Journal of the Korean Society of Clothing and Textiles
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• v.46 no.6
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• pp.1074-1087
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• 2022

This study reports an optimization of a 3D printed wrist brace (WB) for various tilting angles (0°, 45°, 90°) of the re-entrant (RE) pattern and thickness (2 mm, 4 mm) using thermoplastic polyurethane (TPU) filaments and thermoplastic elastomer (TPE) filaments. The actual printing time, weight, Poisson's ratio, and tensile property of the manufactured samples were analyzed. The results confirmed that the actual printing time and weight increased with increasing thickness, regardless of the filament type. All tilting angles of the WB showed a negative Poisson's ratio (NPR), the largest of which appeared at 90°. The results of the tensile property analysis showed that a 90° tilting angle also had the largest value of elongation and stress. From these results, we conclude that the most suitable wrist brace is one in which the actual printing time is low, the weight is minimized to a thickness of 2 mm, and the tilting angle of the RE pattern is 90° for good shock absorption. The choice of filaments may be decided upon according to the user's preference, since the TPU is stiff and the TPE is elastic.

• Proceedings of the Korean Society of Computer Information Conference
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• 2020.07a
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• pp.429-432
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• 2020

본 논문에서는 카메라의 포커싱과 아웃포커싱에 의해 이미지에서 뿌옇게 표현되는 DoF(Depth of field, 피사계 심도) 영역을 합성곱 신경망을 통해 찾는 방법을 제안한다. 우리의 접근 방식은 RGB채널기반의 상호-상관 필터를 이용하여 DoF영역을 이미지로부터 효율적으로 분류하고, 합성곱 신경망 네트워크에 학습하기 위한 데이터를 구축하며, 이렇게 얻어진 데이터를 이용하여 이미지-DoF가중치 맵 데이터 쌍을 설정한다. 학습할 때 사용되는 데이터는 이미지와 상호-상관 필터 기반으로 추출된 DoF 가중치 맵을 이용하며, 네트워크 학습 단계에서 수렴률을 높이기 위해 스무딩을 과정을 한번 더 적용한 결과를 사용한다. 본 논문에서 제안하는 합성곱 신경망은 이미지로부터 포커싱과 아웃포커싱된 DoF영역을 자동으로 추출하는 과정을 학습시키기 위해 사용된다. 테스트 결과로 얻은 DoF 가중치 이미지는 입력 이미지에서 DoF영역을 빠른 시간 내에 찾아내며, 제안하는 방법은 DoF영역을 사용자의 ROI(Region of interest)로 활용하여 NPR렌더링, 객체 검출 등 다양한 곳에 활용이 가능하다.

• Proceedings of the Technology Innovation Conference
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• 1997.07a
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• pp.307-329
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• 1997

Nowadays the whole World is overwhelmed by the wave of Information Technology. In particular, in this Information & Communication Era such advanced countries as United States, Germany, and France are now launching and preparing Government levels Information Technology Policy and Strategies in order to keep their information initiative and superiority. Ever since 1993 when the US government have been advocating m as well as NPR, the US is concentrating their all energies and efforts on the redeem of their former national competitiveness which was snatched up to Japan, meanwhile also Japanese government, who has announced NEW SOCIETY CAPITAL CONSTRUCTION PROGRAM in 1994 to be completed by 2010, is trying to do their whole national endeavors to enter into the highly enhanced information society. Recently also our Government enacted the LAW of INFORMATION TECHNOLOGY PROMOTE & EXPEDITE in 1995 then sequently in the next year announced its detailed enforcement regulations, targeting at one of the leading countries with highly advanced Information Technology. The concept of Internet which takes the concept of Internet into the inner side of the industries has been, since implemented in 1995, showing really the rapid growth and at the same time it is highlighted onto the next generations Information-network. The merits of Internet, representing as the affordable cost, continuous standardization, various functionalities of multimedia excellent expansibilities, easy interface is the most adequate choice as a newest method for our country that announce to be one of leading countries in the realization of Information Technology and expects immediate effect on the construction of Internet in the level of whole country. In an effort to construct the nations level of Internet by firstly the build-up and connection of each central and each local government level of internet, a few methods for nation levels internet build-up are here studied and described, while in the beginnings stage through both e-mails and internet home pages the nation level of internet can be established in stages. The construction of Internet in the level of the nation is not an issue of simple option but an essential and inevitable choice for the survival in 2000s and as well the jump-up and penetration onto the real advanced country in the New Era.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.938-945
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• 2016

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious threats to rice production. In this study, screening of rice for resistance to BLB was carried out at two different times and locations; that is, in a greenhouse during winter and in an open field during summer. The pathogenicity of Xoo race K1 was tested on 32 Korean rice cultivars. Inoculation was conducted at the maximum tillering stage, and the lesion length was measured after 14 days of inoculation. Five cultivars, Hanareum, Namcheon, Samgdeok, Samgang, and Yangjo, were found to be resistant in both the greenhouse and open-field screenings. Expression of the plant defense-related genes JAmyb, OsNPR1, OsPR1a, OsWRKY45, and OsPR10b was observed in resistant and susceptible cultivars by qRT-PCR. Among the five genes tested, only OsPR10b showed coherent expression with the phenotypes. Screening of resistance to Xoo in rice was more accurate when conducted in open fields in the summer cultivation period than in greenhouses in winter. The expression of plant defense-related genes after bacterial inoculation could give another perspective in elucidating defense mechanisms by using both resistant and susceptible individuals.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1250-1258
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• 2009

Bacillus spp., as a type of plant growth-promoting rhizobacteria (PGPR), were studied with regards promoting plant growth and inducing plant systemic resistance. The results of greenhouse experiments with tobacco plants demonstrated that treatment with the Bacillus spp. significantly enhanced the plant height and fresh weight, while clearly lowering the disease severity rating of the tobacco mosaic virus (TMV) at 28 days post-inoculation (dpi). The TMV accumulation in the young non-inoculated leaves was remarkably lower for all the plants treated with the Bacillus spp. An RT-PCR analysis of the signaling regulatory genes Coil and NPR1, and defense genes PR-1a and PR-1b, in the tobacco treated with the Bacillus spp. revealed an association with enhancing the systemic resistance of tobacco to TMV. A further analysis of two expansin genes that regulate plant cell growth, NtEXP2 and NtEXP6, also verified a concomitant growth promotion in the roots and leaves of the tobacco responding to the Bacillus spp.

• The Journal of the Korea Contents Association
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• v.9 no.7
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• pp.102-109
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• 2009

In this paper, we present an algorithm which generates the impossible drawings after the manner of M.C. Escher. A class of the impossible drawings, focused on this paper, depicts the non-realistic configuration such that an ascent (or a descent) looks like keeping on permanently with a height-deceptive loop. We analyze the fact that the ascending direction in the non-realistic illustrations comes not from the physical heights of the objects but from the artist's intended forwarding direction about the loop, which does not have any physical sense of depths. The basic idea to support such impossible drawings is to use a loop of layered depth images (LDIs), where several LDIs are arranged along with the forwarding direction of the loop while having the physically constant heights. The height-deception between two adjacent objects comes from the layer values in the LDIs. In this paper, we propose a NPR system which can manipulate a shape of the loop and layer values of the LDIs and demonstrate several impossible drawings results generated by using our system.

• Nutrition Research and Practice
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• v.2 no.3
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• pp.165-170
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• 2008

Protein-energy malnutrition is regarded as one of the public health problems in developing countries as a result of poor feeding practices due to poverty. This study, therefore, aimed at evaluating nutritional quality of a potential weaning food formulated from locally available food materials. The cooking banana fruit(CB) and bambara groundnut seeds(BG) were purchased from local market in Akure, Ondo State, Nigeria. The CB and BG were processed into flours, mixed in ratios of 90:10, 80:20, 70:30 and 60:40 and subjected into proximate, sensory and biochemical analyses using standard procedures. Nutrend(a commercial formula) and ogi(corn gruel, a traditional weaning food) were used as control. The nutritient composition(g/100 g) of the food samples were ranged as follows: moisture 2.94-6.94, protein 7.02-16.0, ash 1.76-2.99, fat 0.76-8.45, fibre 1.52-3.75, carbohydrate 63.84-88.43 and energy 1569.8-1665.7 kcal. The biological value(BV), net protein retention(NPR), protein efficiency ratio(PER) and feed efficiency ratio(FER) of the experimental food samples were significantly(p<0.05) lower than nutrend, but higher than ogi. The haematological variables of rats fed with formulated food samples, commercial formula(nutrend) and traditional weaning food(ogi) were not significantly(p>0.05) influenced by the dietary treatment. However, the values obtained for red blood cell(RBC), white blood cell(WBC), pack cell volume(PCV) and erythrocyte sedimentation rate(ESR) were higher in the experimental food samples than the commercial food. The growth rate of animals fed with experimental food samples were lower than those fed with the nutrend, but higher than those fed with ogi. In conclusion, the nutritional quality of CB and fermented BG mix of 60:40 ratio was better than ogi; and comparable to the nutrend. This implies that it can be used to replace low quality traditional weaning food and the expensive commercial weaning formula.

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.748-756
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• 2016

In the present study, a lipopeptide (named AXLP14) antagonistic to Xanthomonas oryzae pv. oryzae (Xoo) was obtained from the culture supernatant of Bacillus amyloliquefaciens B014. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that AXLP14 consisted of surfactin homologs. The minimum inhibition concentration and minimum bactericidal concentration of AXLP14 against Xoo were determined to be 1.25 and 2.50 mg/ml, respectively. At a concentration of 0.613 mg/ml, AXLP14 strongly inhibited the formation of Xoo biofilm. AXLP14 also inhibited the motility of Xoo in a concentration-dependent manner. Applying AXLP14 to rice seedlings significantly reduced the incidence and severity of disease caused by Xoo. In Xoo-infected rice seedlings, AXLP14 strongly and continuously up-regulated the expression of both OsNPR1 and OsWRKY45. In addition, AXLP14 effectively inhibited the Xoo-induced up-regulation of the expression of the abscisic acid biosynthesis gene OsNECD3 and the abscisic acid signalingresponsive gene OsLip9, indicating that AXLP14 may protect rice against Xoo-induced disease by enhancing salicylic acid defense and interfering with the abscisic acid response to virulence.

• Journal of Microbiology and Biotechnology
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• v.19 no.2
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• pp.194-203
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• 2009

$HpaG_{Xooc}$, from rice pathogenic bacterium Xanthomonas oryzae pv. oryzicola, is a member of the harpin group of proteins, eliciting hypersensitive cell death in non-host plants, inducing disease and insect resistance in plants, and enhancing plant growth. To express and secret the $HpaG_{Xooc}$ protein in Bacillus subtilis, we constructed a recombinant expression vector pM43HF with stronger promoter P43 and signal peptide element nprB. The SDS-PAGE and Western blot analysis demonstrated the expression of the protein $HpaG_{Xooc}$ in B. subtilis. The ELISA analysis determined the optimum condition for $HpaG_{Xooc}$ expression in B. subtilis WBHF. The biological function analysis indicated that the protein $HpaG_{Xooc}$ from B. subtilis WBHF elicits hypersensitive response(HR) and enhances the growth of tobacco. The results of RT-PCR analysis revealed that $HpaG_{Xooc}$ induces expression of the pathogenesis-related genes PR-1a and PR-1b in plant defense response.