• 생명과학회지
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• 제27권12호
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• pp.1470-1478
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• 2017

본 연구는 건강기능성 식품의 소재로서 활용하기 위해 부안, 남원 및 보은의 산지별 누에분말을 단백질 분해능이 뛰어난 Bacillus subtilis KACC 91157 균주로 발효시켜 제조한 발효누에 추출물의 이화학적 특성 및 생리활성을 평가하였다. 구성 아미노산 함량은 부안 누에군(BU)에서 가장 높았으나, 발효 후에는 보은 발효누에군(BBO)에서 가장 높은 함량을 보였다. 미네랄 함량은 K, Ca, Mg 순으로 함유되어 있었으나, Na가 가장 높은 미네랄 함량의 증가율을 보였다. DPPH를 통한 자유라디칼 소거능과 혈전용해 효소활성은 발효 전에 비하여 발효누에군에서 높게 나타났으며, 특히 BBO군에서 가장 높은 활성을 보였다. 전립선암 세포(LNcaP)와 유방암 세포(MCF-7) 성장 저해능은 BBO군에서 가장 높게 나타났으며 LPS 처리 RAW 264.7세포의 NO생성 억제활성 역시 BBO군에서 가장 높게 나타났다. 이와 같이 산지별 고초균 발효누에 추출물을 비교해본 결과 보은 누에가 건강기능성 식품의 소재로서 활용할 가치가 높은 것으로 사료된다.

• Journal of Plant Biology
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• 제17권1호
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• pp.3-8
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• 1974

The experiments were conducted to examine the effects of sulfur dioxide on catalase activity in germinating seeds under dark conditions and in green plants under light conditions. Three kinds of plants, bean (Glycine max M.), cabbage (Brassica oleracea L. Chungbang No.1) and rice (Oryza sativa L. Suwon No.213-1) were used in this study. Both thegerminating seeds and the leaves were treated with various concentrations of sulfur dioxide for both one green plants with two and twelve hours. In the results of the measurement of catalase activity it was found that plant injuries by sulfur dioxide for the period of photosynthesis were higher than those for the period of respiration. In the control, apparent catalase activity under the light condition of photosynthesis showed a considerable decrease in comparison with catalase activity under the dark condition of respiration. This tendency also appeared markedly in the gas treatment. In the gas treatment for twelve hours, the decrease of catalase activity was higher than that of one hour in both photosynthesis and respiraton. It was thought that sulfur dioxide was an inhibitor of catalase activity in higher concentration of the gas.

• 미생물학회지
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• 제21권2호
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• pp.79-85
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• 1983

Ammonia oxidation activity of polluted water samples in Jinhae Bay and isolated strain from the seawater was investigated, and effects of environmental factors such as temperature, salinity, substrate concentration to the ammonia oxidation were also investigated. The ammonia oxidation activities of sediments, 0.01-0.04mg eq. $NO_2-N/l/h$, were exceptionally higher than that of sea water, $0.5{\sim}1{\mu}g$ eq. $NO_2-N/l/h$. the activities of muddy sediments at station 4 and 2 were 0.03~0.04mg eq. $NO_2-N/l/h$ and that of sandy sediment at station 3 was 0.002mg eq. $NO_2-N/l/h$. In the case of sea water, the activity of polluted area, station 1, was 2 times higher than that of offshore, station 4. The isolated strain reached log phase after 30days culturs and its oxidation activity was $2{\sim}3{\mu}g$ eq. $NO_2-N/day$. The maximum oxidation of ammonia by IA 13 strain occured at 30mg/l oxidation increased with the salinity rising up to 100% seawater concentraion. And temperature for maximum oxidation of ammonia was $35^{\circ}C$. the oxidation increased with the salinity rising up to 100% seawater concentration.

• 대한본초학회지
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• 제26권1호
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• pp.59-63
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• 2011

Objectives : Elastic fibers are found in the skin, lungs, arteries, veins and other structures. The defects of elastic matrix aggravate hypertension which is associated with alteration in the great arteries, arteries, and arterioles. The elastase inhibitors were undergoing in clinical studies about emphysema and pulmonary hypertension. This study was designed to investigate the effect of Astragali Radix extracts (AR) on elastase activity and anti-oxidative effects. Methods : The elastase inhibitory activity and DPPH (1,1-diphenyl-2-picrylhydrazyl) and NO free radical scavenging activities of AR were measured. Results : The elastase activity was significantly inhibited by AR. The significant DPPH and NO free radical scavenging activities were observed in AR as well. Conclusion : AR showed the anti-elastase effects and anti-oxidative activities in vitro. These results suggest that AR may be a possible drug for the treatment of pulmonary emphysema and pulmonary hypertension.

• 사상체질의학회지
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• 제22권1호
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• pp.49-58
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• 2010

1. Objectives: The purpose of this study is to find out the Anti-Oxidative effects of Soyangin Hyeongbangpaedok-san(HBP) in kidney and spleen cells of aged rats. 2. Methods: Aged rats used in this experiment were 6, 52, 68 weeks old. Each age group was divided into three groups again. One group was given no treatment, another group was dosed normal saline and the other group was dosed HBP decoction. The antioxidant effects of HBP decoction were measured by the levels of SOD, GSH, MDA and NO. 3. Results: and Conclusions: 1) The activity of SOD was significantly increased in kidney cells of 68w-HBP group. Deterioration of the activity of SOD in kidney cells was significantly suppressed according to increasing in age of the week. 2) The level of NO was significantly decreased in kidney cells of 68w-HBP group. Deterioration of The level of NO in kidney cells was significantly suppressed according to increasing in age of the week. 3) The activity of SOD was increased in spleen cells of 52w and 68w-HBP group. 4) The level of GSH was significantly increased in spleen cells of 68w-HBP group.

• 대한의생명과학회지
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• 제25권2호
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• pp.196-200
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• 2019

Effect of retinoids, i.e., all-trans retinoic acid and 13-cis retinoic acid, on the activity of nitric oxide synthase (NOS) was evaluated in human malignant keratinocytes to examine the possible correlation of retinoids with NOS activities. All-trans retinoic acid and 13-cis retinoic acid did not alter the nitric oxide (NO) production. However, in the presence of lipopolysaccharide (LPS, $1{\mu}g/mL$), they significantly increased NO release in a dose-dependent manner until 48 h at concentrations of $50{\sim}100{\mu}M$. The degree of upregulation of NO by all-trans retinoic acid and 13-cis retinoic acid increased up to 35% and 37%, respectively, compared to that by the control, which demonstrated the upregulation of LPS-inducible nitric oxide synthase (iNOS)-dependent generation of NO as well as showing a crucial link between retinoids-induced activity and NOS. Findings of this study now suggest that the upregulation of LPS-iNOS activity may be associated with modulation of retinoids-induced control of cellular developmental processes, which may produce new therapeutics of retinoids in the complexity of how NO affects human keratinocytes.

• 한국안전학회지
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• 제6권3호
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• pp.5-11
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• 1991

To remove NO from flue gas, a direct decomposition method to $N_2$ and $O_2$ was investigated by using copper / zeolite catalyst. The copper ion-exchanged HY type zeolite has high activity on NO decomposition. The decomposition activity was increased with the increase of ion-exchange level, contacting time and reaction temperature in the range of 30$0^{\circ}C$ -50$0^{\circ}C$ , and decreased with the oxygen addition.

• 약학회지
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• 제59권1호
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• pp.12-16
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• 2015

Several 8-cycloalkoxychrysin analogs were synthesized from 6-benzyloxy-5,8-dihydroxyflavone and cycloalkanols in 2 steps and we evaluated their inhibitory activities against NO production from LPS-induced RAW 264.7 cells. Among tested analogs, two analogs with cyclopentyl substructure (796 and 798) showed strong inhibitory activity against NO production.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1617-1622
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• 2005

The activity of brush border enzymes (sucrase, lactase and maltase) in the piglet small intestine was evaluated as well as piglet performance during the weaning period in the present study. There were two treatment groups: Piglets of six litters were fed dry feed plus milk replacer (Group M) and of six litters fed dry pelleted feed (Group C). One piglet from each litter was sacrificed on day 3 before weaning, and day 3, 10 and 17 postweaning, respectively. Providing milk replacer caused an increased piglet live weight at weaning (p<0.001) and until termination of the experiment (p<0.001). A slightly higher (p<0.16) level of protein was measured in the jejunum of group M piglets as compared with group C piglets. Before weaning the activity of lactase was high in the jejunum of group C piglets. The activity of lactase in the jejunum was lowered in the jejunum of group C piglets and in distal jejunum of group M piglets during the postweaning period as compared with pre-weaning period (p<0.05). Lowered activity of lactase in the distal jejunum of piglets was found at day 10 and 17 postweaning, respectively. No treatment differences were found in the activity of lactase in the piglet jejunum. No treatment differences were seen in the activity of maltase and sucrase in the piglet jejunum also. However, weaning caused a higher activity of sucrase in the distal jejunum of group M piglets as compared with pre-weaning period. In conclusion, providing milk replacer to piglets caused an improved growth performance. Feeding milk replacer did not influence the activity of lactase, maltase and sucrase in the jejunum of piglets. Weaning resulted in a markedly lowered activity of lactase, while no dramatic changes in the activity of maltase took place during the period around weaning.

• 생약학회지
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• 제38권2호통권149호
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• pp.170-175
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• 2007

Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO$^-$), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. We tried to evaluate the effects of two kinds of varieties of Perilla frutescens var japnica Hara on the NO production in lipopolysaccharide (LPS)-activated microglia. The perilla cultivars of Namcheondeulkkae (NC) and Boradeulkkae (BR) were developed by pure line from the local variety and by a cross between 'deulkkae' and 'chajogi', respectively. Spirit, hexane, chloroform and butanol fractions of the leaves of NC and BR inhibited the production of NO in LPS-activated microglia. The fractions of BR showed stronger activity than NC and the spirit extracts was the most potent in both cultivars. The solvent fractions of BR suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells. Moreover, the extracts of NC and BR showed the activity of peroxynitrite scavenging in cell free bioassay system. These results imply that Namcheondeulkkae and Boradeulkkae might have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity.