• Journal of Dairy Science and Biotechnology
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• v.35 no.3
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• pp.202-207
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• 2017

The objective of this study was to analyze the effects of homogenization, storage temperature, and storage period on the creaming of milk fat and changes in fat contents in the upper and lower layers and to predict the conditions for optimal homogenization efficiency using response surface methodology (RSM). The homogenization pressure, storage temperature, and storage period were set as independent variables of RSM, and the dependent variables were creaming, US Public Health Service (USPHS) code, and volume weighted mean diameter ($D_{4,3}$) in the upper and lower layers. Based on the results of RSM and regression analysis, the correlation coefficient ($R^2$) between experimental data and predicted values by RSM for homogenized milk was estimated to be more than 0.8. The RSM analysis indicated that optimal homogenization pressures of 14 MPa or more and 17 MPa or more were required to maintain the creaming layer of 3 mm or less during the storage for 15 days at $10^{\circ}C$ and $20^{\circ}C$, respectively. To keep the USPHS code at less than 10% for 15 days at $10^{\circ}C$ and $20^{\circ}C$, milk should be homogenized with a pressure of 16.8 MPa or more and 17 MPa or more, respectively.