• Asian-Australasian Journal of Animal Sciences
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• v.31 no.4
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• pp.595-606
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• 2018

Objective: The Fusarium mycotoxins of deoxynivalenol (DON) and zerolenone (ZEN) cause health hazards for both humans and farm animals. Therefore, the main intention of this study was to reveal DON and ZEN effects on the mRNA expression of pro-inflammatory cytokines and other immune related genes in the liver of piglets. Methods: In the present study, 15 six-week-old piglets were randomly assigned to the following three different dietary treatments for 4 weeks: control diet, diet containing 8 mg DON/kg feed, and diet containing 0.8 mg ZEN/kg feed. After 4 weeks, liver samples were collected and sequenced using RNA-Seq to investigate the effects of the mycotoxins on genes and gene networks associated with the immune systems of the piglets. Results: Our analysis identified a total of 249 differentially expressed genes (DEGs), which included 99 upregulated and 150 downregulated genes in both the DON and ZEN dietary treatment groups. After biological pathway analysis, the DEGs were determined to be significantly enriched in gene ontology terms associated with many biological pathways, including immune response and cellular and metabolic processes. Consistent with inflammatory stimulation due to the mycotoxin-contaminated diet, the following Kyoto encyclopedia of genes and genomes pathways, which were related to disease and immune responses, were found to be enriched in the DEGs: allograft rejection pathway, cell adhesion molecules, graft-versus-host disease, autoimmune thyroid disease (AITD), type I diabetes mellitus, human T-cell leukemia lymphoma virus infection, and viral carcinogenesis. Genome-wide expression analysis revealed that DON and ZEN treatments downregulated the expression of the majority of the DEGs that were associated with inflammatory cytokines (interleukin 10 receptor, beta, chemokine [C-X-C motif] ligand 9), proliferation (insulin-like growth factor 1, major facilitator superfamily domain containing 2A, insulin-like growth factor binding protein 2, lipase G, and salt inducible kinase 1), and other immune response networks (paired immunoglobulin-like type 2 receptor beta, Src-like-adaptor-1 [SLA1], SLA3, SLA5, SLA7, claudin 4, nicotinamide N-methyltransferase, thyrotropin-releasing hormone degrading enzyme, ubiquitin D, histone $H_2B$ type 1, and serum amyloid A). Conclusion: In summary, our results demonstrated that high concentrations DON and ZEN disrupt immune-related processes in the liver.

• Korean Journal of Microbiology
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• v.35 no.3
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• pp.218-225
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• 1999

We isolated the ANRI fragment from Aspergillus nidulons that could autononlously replicate and enhance transformation efficiency about $10^4$ fold compared lo the integrative vector in Saccha,omgcer cerevisioe. In A. nidulans recombinant plasmid pLJ16-4.5 which carries the 4.5 kb EcoRI fragment of ANRI showed a 170-[old increase of transformation efficiency compared to the integrative vector pLJ16 and could be recovered from iransfonnants as an intact form. Estimated copy number of transforming plasmid pLJ16-4.5 was scored as 2 to 3 copies in transformed A. nidulans. Recoinbinant plasinid pILJ16-4.5 is inilotically unstable; being lost Irom 65% of aswual progeny of transformants on selective medium and 90% on complete medium. Southern analysis of transformant DNA showed that the pILJ16-4.5 is maintained in free form. The sequencing data showed that ANRl fragment was originated from mitochondiral DNA of A. nid~ilans and contained high AT content as much as 74.7%. One ARS consensus sequence (A/T)TTr4T(A/G)TTT(AiT). I I ARS-like sequence (agreement 10 of 11) and ABFl binding core consensus sequence (TCN7ACG). Also six gyrase binding core consensus sequence (YRTGNYNNY: y=C or T, R=A or G, N=A, G, C or T) of $\Phi$X174 and SV40 DNA and one b site (CACTTTACC) combining with gyrase in ColEl are shown. ANRl can be developed as a repl&ng plasinid for lransfoimation system in A. nirlulmis.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.637-646
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• 2005

Immunization of layers against Salmonella gallinarum(S.G.) which causes fowl typhoid resulted in production of anti-S.G. IgY rich eggs. Water soluble fraction was obtained from egg yolk using various gum solutions such as 0.1%(Sigma C-3889) λ-carrageenan; 1% and 2% cold water soluble carrageenan; 1% and 2% hot water soluble carrageenan; and 1% cold water soluble carrageenan with 1% hot water soluble carrageenan. Among them, λ-carrageenan 0.1% treatment showed a high recovery rate, possessing high IgY contents. In the range of pH 5-9, more than 70 percent of IgY was existent. Moreover, Anti-S.G. IgY was relatively heat-stable. This study revealed that immunoglobulin against fowl typhoid could be produced successfully by layers and the IgY was sustainable to further processing due to its pH and heat stability. IgY is promising to be utilized for prevention and treatment of fowl typhoid in industrial scale.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2002.11a
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• pp.29-29
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• 2002

공구강 등 산업용 재료로 널리 사용되는 카바이드 계 재료는 입자 크기 및 분포에 따라 기계적 성질이 변화하므로, 이를 제어하고 조절하는 기술에 관하여 많은 연구가 진행되어 왔다. 본 연구에서는 TiCN-WC-Co 복합초경계 에서 소결 공정 및 조성변화에 따른 입자 모양을 관찰하고 이에 따른 업자 성장 거동을 고찰하였다. 일반적으로 입자 조대화 양상과 고상 입자의 모양과는 밀접한 관계가 있다. 각진 입자의 경우에 는 계면이 원자적으로 singular 하여 원자의 홉착이 어렵기 때문에 임계값 이상의 성장 구동력을 받 는 몇몇 입자만 성장하는 비정상 입자 성장이 일어날 수 있다. 반면에 계면이 rough한 퉁큰 엽자의 경우에는 원자 홉착에 필요한 구동력이 존재하지 않아 성장 구동력을 받는 모든 입자들이 성장하기 때문에 정상 입자 성장을 하게 된다. 이와 같이 입자 모양에 따른 입자 성장 거동은 전체 미세구조를 결정하게 되며, 이에 따른 물리 화학적 물성을 변화시킨다. 이러한 입자 성장 원리를 적용하 면 복합초경계 (TiCN-WC-Co)에서도 입자성장이 억제되고 치밀한 소결체를 제조할 수 있을 것이다. 본 실험에서는 평균입도가 각각 0.1, 1.33, 2$\mu\textrm{m}$인 TiCN, WC, Co 분말을 사용하여 $((I00_{-x)}TiCN+_xWC)-30Co$ (wt%) 조성에서 TiCN/WC 비를 변화시키면서 업자 모양과 입자성장 거동을 관찰하였다. 청량된 분말은 WC 초경 볼로 밀렁하고, 건조한 후, 100 mesh 체로 조립화 하였다. 이 분말을 100 MPa의 압력으로 냉간정수압성형 하고 $10^{-2}$ torr의 진공분위기의 graphite f furnace에서 carbon black으로 packing 하여 액상형성 온도 이상에서 소결하였다. 소결된 시편은 경면 연마하여 주사전자현미경으로 미세 조직을 관찰하였다. TiCN-30Co 조성 시편은 corner-round 모양의 입자 모양으로 소결 시간 증가에 따라 빠른 입자 성장을 나타내었다 .(7STiCN+2SWC)-30Co 조성 시변의 경우 일반적으로 보고된 바와 같이 core/shell 구조를 나타내었으며, core는 TiC-rich 상이었고, shell은 (Ti,W)(C,N) 복합 탄화물 상이었다. WC 함량이 중가함에 따라 입자의 corner-round 영역이 증가하였으며 (SOTiCN-SOWC)-30Co 조성 근처에서는 거의 둥근 형태의 입자 모양을 나타내었다. 또한 TiCN - 30Co 조성 시편에 비하여 WC가 첨가된 시펀들은 작은 평균입자크기를 나타내었다. 본 연구의 결과는 shell 영역 조성 변화는 계면에너지 이방성과 기지상 내의 펑형 입자 모양을 변화시키고 나아가 입자 성장 속도 에도 영향을 미친다는 것을 보여준다.

• Korean Journal of Environmental Agriculture
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• v.10 no.1
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• pp.11-19
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• 1991

This study was carried out to develop the low-priced adsorbent by synthesizing the zeolite of high CEC with the natural zeolite and examining the ability of this zeolite to adsorb heavy metals. The dominant clay minerals were clinoptilolite and mordenite in natural zeolite, while phillipsite in the synthesized zeolite. Adsorption reaction of Cu and Zn on clays were reached to equilibrium after 1 hr. The amount of adsorption was increased as the concentrations of heavy metals or the initial pH of suspension was increased. The synthesized zeolite adsorbed heavy metals about twice as much as the natural zeolite. The adsorption of heavy metals on the synthesized zeolite was less affected by the initial pH of suspension than that on natural zeolite. At cumulative adsorption, the synthesized zeolite adsorbed much more heavy metals at early three treatments than the natural zeolite did. The amount of desorption by chloride salts was increased as the concentration of chloride salts was increased. The ability of salt to desorb was in the order of NaCl>$CaC1_2$>$AlC1_3$. It is estimated that the ability of the synthesized zeolite to remove heavy metals was better than that of the natural zeolite.

• Economic and Environmental Geology
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• v.27 no.4
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• pp.375-385
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• 1994

The studied area is composed of Precambrian gneiss complex, middle Jurassic biotite granite, late Cretaceour sediments, volcanics and pink feldspar granite. Characteristic minerals of the biotite granite is plagioclase and hornblende whereas the pink feldspar granite is pink feldspar (perthite) and quartz. Plagioclase compositions of the biotite granite and the pink feldspar granite are oligoclase to calcic andesine ($An_{18-44}$) and sodic albite ($An_{0.5-5.0}$), respectively. In the variation diagrams of the Harker and normative Q-Or-Pl diagram, the biotite granite belongs to the category from granodiorite to granite, the pink feldspar granite from nomal to late granite. The values of D.I. L.I. and alkalinity of the pink feldspar granite are higher than those of the biotite granite. While CaO is enriched in the biotite granite, $K_2O$ is enriched in the pink feldspar granite. The ratio of $K_2O/Na_2O$ which indicates the relative ratio of alkali is 1.06 in the pink feldspar granite, and 0.86 in the biotite granite. In A-M-F and N-C-K diagrams both these granites are plotted in peraluminus granite ($Al_2O_3$>$Na_2O+K_2O+CaO$) region, assigned to calc alkaline series and alkaline series respectively. Put into the form of A-C-F diagram, the biotite granite falls under I-type, and the pink feldspar granite S-type. On the base of whole rock ratios of $Fe^{+3}/Fe^{+2}+Fe^{+3}$ and $^{87}Sr/^{86}Sr$ for the granites in studied area, the biotite granite indicates ilmenite series (0.26) and S-type and/or contaminated I-type ($0.72020{\pm}0.00050$), the pink feldspar granite magnetite series (0.44) and I-type ($0.70826{\pm}0.00020$).

• Journal of Biosystems Engineering
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• v.1 no.1
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• pp.55-55
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• 1976

An experimental work was conducted to develop an optimum operating system of various hay drying systems ; sun-drying with long hay, sun-drying after chopping, sun-drying after crushing, heated air drying after chopping using batch-type dryer and heated air drying after crushing using tunnel-type dryer. Seombody having 60 cm long and initial moisture content of approximately 79 % in wet basis was used for the experiment. The criteria selected for determining the optimum operating condition were the drying performance rate, the production cost and quality of dried matter of each drying systems. The result of this study are summarized as follows : 1. Drying characteristics of leaves of long stem hay, chopped seombody and crushed one were obtained by maintaining the oven temperature at 70 degrees centigrade. The required drying times for various samples to approximately 15% moisture content in wet basis were about 50 min .for leaves ; 160 min. for crushed hay ; 250 min. for chopped hay ; 340min. for ling hay and more than 360 min .for stems. The drying time of crushed hay was required about 50 % of that for the uncrushed long hay. Such a significant difference of drying of time between the leaf and long stem may indicate that an effective drying of seombody may not be achieved unless any kind of special process treatment for the whole hay is undertaken. 2. In each individual drying system, the following conclusions were drawn: a. After 8 days sun-drying on concrete floor under good days with average tempe?rature at $256{\circ}C$ and relative humidity at 55% at 2 P.M., the moisture content of long hay was still above 25 5'~ and the leaf loss during drying caused by wind and rough handling was more than 50 ~G. b. It was possible to dry the chopped seombody by sun-drying down to about 10 % moisture content within 5 days, however, a stock of heat and discolouration phenomena were observed during the drying, which may be due to the increased deposit-density by chopping, resulting in lowering the quality of the dried product. c. Sun-drying for the crushed material by hay-conditioner was required about 4 days to reduce the moisture content to about 10 %, keeping the quality of dried product at good grade. o. The optimum deposit-depth of the chopped seombody in the batch-type dryer used was about 28cm with about 42kg/hr of drying performance rate. However, it was necessary to overturn the materials between the upper and lower layers in order to obtain a good quality of dried product. d. The drying performance rate by the tunnel-type drier was highest among those of drying systems tested, giving the rate of approximately 400kg/day. 3. On reviewing the individual drying system for seombody, it was possible to draw conclusion that the best system was tunnel drying with the crushed seombody as far as the performance rate was concerned. However, the methods gives the highest operational cost. The system for the lowest operational cost with good quality of dried product was the sun-drying with the crushed material. Accordingly, it may be recommended that the system of sun-drying for the crushed seombody may be the most feasible system presently applicable to farm-level operation.

• Journal of Embryo Transfer
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• v.20 no.2
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• pp.163-168
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• 2005

The objective of this study was to produce superior cows by sexual distinction of embryos collected from the donor with pedigree information. Collected embryos distinguished by biopsy using punching or bisection methods and Loop-mediated isothermal amplification of sex-determined DNA for sexing. Six embryos predicted as female were transplanted to recipients and then 2 pregnant cows were normally delivered of calves at 278 and 285 days after embryo transfer. Birth weights of calves named Barani and Borani were 18kg and 25kg, respectively. Adjusted body weights for 90 days were 61.1kg and 88.8kg, respectively. Average daily gains were 0.48kg and 0.71kg, respectively.

• Korean Journal of Food Science and Technology
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• v.43 no.4
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• pp.469-474
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• 2011

Ginger (Zingiber officinale Rosc.) is a well known edible plant that is traditionally used to treat various illnesses related to inflammation and oxidative stress. Steam distillated ginger oil or water extract are mainly used for related products; however, it is unclear whether these fractions contain most of the bioactive compounds or the highest efficacy. This investigated the antioxidant effects of extracts prepared by supercritical fluid extraction (SFE). 6-Gingerol was the most abundant component in hexane fraction of ethanol extract from ginger. The antioxidative properties of SFE oil and Marc ethanol fractions were demonstrated using the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azinobis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals scavenging assays. A clear correlation was observed between total polyphenol contents and $RC_{50}$ values in the Marc ethanol fraction. These results indicate that not only SFE oil but the marc after SFE could be good sources for the food industry.

• The Korean Journal of Pharmacology
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• v.32 no.2
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• pp.189-199
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• 1996

Myocardial ${\alpha}_1-adrenoceptors$ have been shown to mediate a biphaslc inotropic response that was characterized by a transient decline followed by a sustained increasing phase in guinea pig ventricular muscle. Recently one group reported that an ${\alpha}_1-adrenoceptors-induced$ intracellular $Na^+$ decrease is linked to fast $Na^+$ channel inhibition and another group reported that it is linked to $Na^+$-$K^+$ pump activation by ${\alpha}_{1b}-adrenoceptors$. But until now, its mechanism is not clear. Therefore, to see whether the $Na^+$channel or $Na^+-K^+$ pump is related to a decrease in intracellular $Na^+$ activity and/or the negative inotropic response, and which ${\alpha}_1-adrenoceptor$ subtype was involved in the decrease in intracellular $Na^+$activity by phenylephrine, we used conventional and sodium selective microelectrodes, and tension transducer to determine the effects of ${\alpha}_1-adrenergic$ stimulation on membrane potential, intracellular $Na^+$ activity, and twitch force in guinea pig ventricular muscles. $10^{-5}$ M Phenylephrine produced a slight hyperpolarization of the diastolic membrane potential, a decrease or increase in $a_N^i_a$, and a biphasic inotropic response. The negative inotropic response accompanied by a decrease in intracellular $Na^+$activity, whereas in muscles showing a remarkable positive inotropic response without initial negative inotropic effect was accompanied by an increase in intracellular $Na^+$ activity. The decrease in intracellular $Na^+$ activity was apparently inhibited by WB4101, an antagonist of the ${\alpha}_{1a}-adrenoceptors$. The decrease in intracellular $Na^+$ activity caused by phenylephrine was not abolished or reduced by a block of the fast $Na^+$ channels. $V_{max}$ also was not affected by phenylephrine. Phenylephrine produced an increase in intracellular $Na^+$ activity in the presence of a high concentration of extracellular $Ca^{2+}$ (in quiescent muscle) or phorbol dibutyrate, a protein kinase C activator(in beating muscle). These suggest that the ${\alpha}_{1a}-adrenoceptors-mediated$ decrease in intracellular $Na^+$ activity may be related to the protein kinase C.