• 제목/요약/키워드: N utilization

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Overexpression of Mutant Galactose Permease (ScGal2_N376F) Effective for Utilization of Glucose/Xylose or Glucose/Galactose Mixture by Engineered Kluyveromyces marxianus

  • Kwon, Deok-Ho;Kim, Saet-Byeol;Park, Jae-Bum;Ha, Suk-Jin
    • Journal of Microbiology and Biotechnology
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    • 제30권12호
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    • pp.1944-1949
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    • 2020
  • Mutant sugar transporter ScGAL2-N376F was overexpressed in Kluyveromyces marxianus for efficient utilization of xylose, which is one of the main components of cellulosic biomass. K. marxianus ScGal2_N376F, the ScGAL2-N376F-overexpressing strain, exhibited 47.04 g/l of xylose consumption and 26.55 g/l of xylitol production, as compared to the parental strain (24.68 g/l and 7.03 g/l, respectively) when xylose was used as the sole carbon source. When a mixture of glucose and xylose was used as the carbon source, xylose consumption and xylitol production rates were improved by 195% and 360%, respectively, by K. marxianus ScGal2_N376F. Moreover, the glucose consumption rate was improved by 27% as compared to that in the parental strain. Overexpression of both wild-type ScGAL2 and mutant ScGAL2-N376F showed 48% and 52% enhanced sugar consumption and ethanol production rates, respectively, when a mixture of glucose and galactose was used as the carbon source, which is the main component of marine biomass. As shown in this study, ScGAL2-N376F overexpression can be applied for the efficient production of biofuels or biochemicals from cellulosic or marine biomass.

한우육의 육질등급 및 부위 별 지방산 조성 비교 (Comparison of Fatty Acid Composition of Hanwoo Beef by Different Quality Grades and Cuts)

  • 이연정;김천제;김진형;박범영;성필남;강근호;김동훈;조수현
    • 한국축산식품학회지
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    • 제30권1호
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    • pp.110-119
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    • 2010
  • 본 연구는 수소 및 거세우(24-30개월) 15두(각 육질등급별 3두씩)를 공시 축으로 이용하여 한우육의 부위(목심, 채끝, 우둔, 양지머리, 등심)에 따른 육질등급($1^{++}$, $1^+$, 1, 2, 3)의 지방산 조성을 비교하였다. 지방산 조성에서 C16:0 함량은 목심, 채끝과 우둔부위 3등급이 다른 등급보다 유의적으로 높았던 반면 양지머리와 등심부위에서는 2등급이 각각 28.26%, 28.74%로 3등급 26.16%, 25.30%보다 유의적으로 높게 나타났다(p<0.05). C18:1n9 함량은 목심 부위에서는 유의적인 차이가 나타나지 않았지만 채끝, 우둔, 양지머리와 등심부위에서는 $1^{++}$등급이 3등급보다 유의적으로 높게 나타났다(p<0.05). C18:3n3 함량은 채끝, 우둔과 목심 부위에서는 유의적인 차이가 나타나지 않았고 양지머리부위에서 $1^{++}$등급이 0.15%로 다른 등급보다 높았는데 등심부위에서는 3등급이 0.23%로 유의적으로 높게 나타났다(p<0.05). C18:3n6 함량은 목심과 등심부위에서만 유의적인 차이가 나타났는데 2등급이 다른 등급보다 유의적으로 높았다(p<0.05). SFA 함량은 다섯 부위에서 $1^{++}$ 등급이 3등급보다 유의적으로 낮은 반면에 MUFA 함량은 $1^{++}$등급이 3등급보다 유의적으로 높게 나타났고(p<0.05) PUFA 함량은 3등급이 다른 등급보다 유의적으로 높았다(p<0.05). n-3 계열 불포화지방산 함량은 목심, 채끝과 우둔부위에는 유의적인 차이가 없었으나 양지머리부위는 $1^{++}$ 등급이 0.15%로 다른 등급보다 높았던 반면 등심부위는 3등급이 0.23%로 다른 등급보다 유의적으로 높았다(p<0.05). n-6 계열 불포화지방산은 5 부위에서 3등급이 다른 등급보다 유의적으로 높은 함량을 나타냈다(p<0.05).

Dietary phosphorus deficiency impaired growth, intestinal digestion and absorption function of meat ducks

  • Xu, Huimin;Dai, Shujun;Zhang, Keying;Ding, Xuemei;Bai, Shiping;Wang, Jianping;Peng, Huanwei;Zeng, Qiufeng
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권12호
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    • pp.1897-1906
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    • 2019
  • Objective: An experiment was conducted to investigate the effects of dietary non-phytate phosphorus (nPP) deficiency on intestinal pH value, digestive enzyme activity, morphology, nutrient utilization, and gene expression of NaPi-IIb in meat ducks from 1 to 21 d of age. Methods: A total of 525 one-d-old Cherry Valley ducklings were fed diets (with 7 pens of 15 ducklings, or 105 total ducklings, on each diet) with five levels of nPP (0.22%, 0.34%, 0.40%, 0.46%, or 0.58%) for 21 d in a completely randomized design. Five experimental diets contained a constant calcium (Ca) content of approximately 0.9%. Body weight (BW), body weight gain (BWG), feed intake (FI), and feed to gain ratio (F:G) were measured at 14 and 21 d of age. Ducks were sampled for duodenum and jejunum digestion and absorption function on 14 and 21 d. Nutrient utilization was assessed using 25- to 27-d-old ducks. Results: The results showed ducks fed 0.22% nPP had lower (p<0.05) growth performance and nutrient utilization and higher (p<0.05) serum Ca content and alkaline phosphatase (ALP) activity. When dietary nPP levels were increased, BW (d 14 and 21), BWG and FI (all intervals), and the serum phosphorus (P) content linearly and quadratically increased (p<0.05); and the jejunal pH value (d 14), duodenal muscle layer thickness (d 14), excreta dry matter, crude protein, energy, Ca and total P utilization linearly increased (p<0.05); however, the serum ALP activity, jejunal $Na^+-K^+$-ATPase activity, and duodenal NaPi-IIb mRNA level (d 21) linearly decreased (p<0.05). Conclusion: The results indicated that ducks aged from 1 to 21 d fed diets with 0.22% nPP had poor growth performance related to poor intestinal digestion and absorption ability; but when fed diets with 0.40%, 0.46%, and 0.58% nPP, ducks presented a better growth performance, intestinal digestion and absorption function.

Nutrient Synchrony: Is it a Suitable Strategy to Improve Nitrogen Utilization and Animal Performance?

  • Yang, Ji-Young;Seo, J.;Kim, H.J.;Seo, S.;Ha, Jong-K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권7호
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    • pp.972-979
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    • 2010
  • The objective of this paper was to review recent studies on nutrient synchrony and the effects of synchronization of energy and N supply in the rumen on nitrogen utilization and animal performance. Theoretically, synchronization of energy and N supply in the rumen should allow more efficient use of nutrients by rumen microbes, increase microbial protein and fermentation end products, and thus increase available nutrients in the small intestine. Efficient use of nutrients possibly improves animal performance and reduces nutrient excretion to the environment. However, a number of studies showed contradictory results in microbial protein synthesis, nitrogen retention and animal production performance. Since there are additional challenges to nutrient synchrony that must be addressed, further research is required to apply the nutrient synchrony concept directly to the field situation.

Effects of rumen-protected biotin on the growth performance, nitrogen utilization and blood parameters of yearling Liaoning cashmere doelings

  • Haiying Liu;Ying Lin;Xuhui Chen;Guiqin Yang
    • Animal Bioscience
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    • 제36권11호
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    • pp.1685-1692
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    • 2023
  • Objective: This study was conducted to investigate the effects of rumen-protected biotin (RPB) on growth performance, nutrient digestibility, nitrogen utilization and plasma biochemical parameters of Liaoning cashmere goats during the cashmere fiber growing period. Methods: Sixteen 6-month-old Liaoning cashmere twin-doelings (24.8±1.20 kg) were allocated to 2 diet groups that were individually ad libitum fed 30% concentrate and 70% forage diet (dry matter [DM]) by a paired experimental design. Goats of the control group were fed the basal diet, while goats belonging to the RPB group were fed the basal diet with 10 mg RPB/d per animal. The duration of the experiment was 16 weeks with two 8-week periods. Digestibility was determined at weeks 7 and 15, and other measures were taken every four weeks. Results: Compared with the control group, the average daily gain of the RPB group increased by 10.94% (p<0.05), and the intake of neutral detergent fiber was increased (p = 0.045). There were some increasing tendencies for the intake of DM, acid detergent fiber and ether extract (p = 0.070, 0.088, and 0.070, respectively). The intake and digestibility of N tended to increase (p = 0.062 and 0.093, respectively), while the N fecal excretion percentage of N intake was decreased (p = 0.093) in the RPB compared with the control group. N retention tended to increase (p = 0.084) with the addition of adding RPB to the diet. Plasma total protein was increased (p = 0.037), whereas the urea-N concentration was decreased (p = 0.049) in the RPB diet group compared with the control diet group. The levels of propionyl-CoA carboxylase (p<0.001) and methylmalonyl-CoA (p = 0.013) were increased in the RPB group. Conclusion: Supplementation of rumen-protected biotin in the diet of cashmere goats can enhance the utilization of N and improve daily weight gain during cashmere fiber growing period.

Atmospheric CO2 enrichment reduces wheat nitrate utilization and enhances soil N2O emissions

  • Hu, Shuijin
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.4-4
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    • 2017
  • Atmospheric carbon dioxide enrichment ($eCO_2$) often increases soil nitrous oxide ($N_2O$) emissions, but the underlying mechanisms are not fully understood. Emerging evidence suggests that $eCO_2$ alters plant N preference in favor of ammonium ($NH_4{^+}-N$) over nitrate ($NO_3{^-}-N$). Yet, whether and how this attributes to the enhancement of $N_2O$ emissions has not been investigated. We examined the effects of $eCO_2$ on soil $N_2O$ emissions in the presence of two N forms ($NH_4{^+}-N$ or $NO_3{^-}-N$), using wheat (Triticum aestivum L.) as a model plant. Our results showed that N forms dominated $eCO_2$ effects on plant and microbial N utilization, and thus soil $N_2O$ emissions. Elevated $CO_2$ significantly increased the rate and the sum of $N_2O$ emissions by three to four folds when $NO_3{^-}-N$, but not $NH_4{^+}-N$, was supplied. Enhanced $N_2O$ emission was related to the reduced plant $NO_3{^-}-N$ uptake in wheat. We propose a new conceptual model in which $eCO_2$-inhibition of plant $NO_3{^-}-N$ uptake and/or $CO_2$-enhancement of soil labile C enhances the N and/or C availability for denitrifiers and increases the intensity and/or the duration of $N_2O$ emissions. Together, these findings suggest that to enhance plant N use efficiency and reduce $N_2O$ emission, crop breeding and management need to consider altered plant preference of N sources under future $CO_2$ scenarios.

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Availability and Utilization of Library Portal Services for Research in University Libraries in Nigeria

  • Ejikeme, Anthonia Nwamaka;F., Obayi Uche.;Ukamaka, Eze Jacintha
    • International Journal of Knowledge Content Development & Technology
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    • 제11권1호
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    • pp.49-64
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    • 2021
  • This research paper delved into the availability and utilization of library portal services for research in university libraries in Nigeria. Two research questions and two null hypotheses were formulated to guide the study. A structured questionnaire was used for data collection. The study was carried out in Nnamdi Azikiwe Library, University of Nigeria Nsukka (UNN) and Felix Aghagbo Nwako Library, Nnamdi Azikiwe University Library (UNIZIK), Awka. A total of 70 professionals (librarians and system analysts) in these libraries supplied data for the study. Data collected was analyzed using mean and standard deviation. These were used to answer the research questions while the null hypotheses were tested using t-test statistic at 0.05 level of significance and 68 degree of freedom. Major findings showed that portal services available in the library showed that the areas of availability of library portal services in Nnamdi Azikiwe Library, U.N.N. includes Agriculture, Health Sciences, Engineering, Environmental Sciences and information about institution, Social Sciences, Arts and Humanities, Natural Sciences. In Festus Aghagbo Nwako Library, Awka, the areas of Portal Library Services include Career Development, Federal Government Programs, Environmental Sciences, Biological Sciences, Social Sciences, Arts and Humanities, Engineering, and Health Sciences. Findings on extent of utilization of portal services available in the libraries indicated that portal services are utilized to a low extent in the university libraries. Furthermore, librarians did not significantly differ in their opinions on the availability of library portal services and on the extent of utilization of library portal services in the libraries under study. It is therefore recommended that provisions should be made by the universities and library management to provide and update required portal services in addition to creating enabling environment for enhanced access and utilization of these services. The governments must make an effort to provide funds for policy implementation, necessary technology training for librarians and users, and develop general information infrastructure.

Dried Bacillus subtilis Culture Reduced Ammonia Gas Release in Poultry House

  • Santoso, U.;Ohtani, S.;Tanaka, K.;Sakaida, M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권5호
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    • pp.806-809
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    • 1999
  • The present study showed the advantages of dried Bacillus subtilis culture (DBSC) supplementation on reducing ammonia gas release in the poultry house. In Experiment 1, 65-week-old Hyline W-36 hens were raised in individual wire-floor cages in a windowless house, and divided into two groups of 180 hens each. One group was fed diets without DBSC as the control and another group was fed a diet supplemented with 2% DBSC. In Experiment 2, 2-week-old broiler chicks were divided into 3 treatment groups of 20 chicks each and maintained in individual floor cages. One group was fed the diet without DBSC and other two groups were fed the diet supplemented with 1 or 2% DBSC, respectively. In experiment 1, DBSC consistently reduced ammonia gas release in the laying house (p<0.01) and manure storage facilities (p<0.01). incubation of feces for 1, 2, 3, 4, 5, 6, 24 or 48 hours showed that DBSC consistently reduced ammonia gas release. In Experiment 2, DBSC reduced ammonia gas release in the broiler house; however, DBSC had no effect on total N, urate-N and ammonia-N contents of feces, but it improved cumulative N utilization and decreased serum urea-N concentration when chicks when chicks were fed 1% DBSC.

Effect of Feeding Rubber Seed Kernel and Palm Kernel Cake in Combination on Nutrient Utilization, Rumen Fermentation Characteristics, and Microbial Populations in Goats Fed on Briachiaria humidicola Hay-based Diets

  • Chanjula, P.;Siriwathananukul, Y.;Lawpetchara, A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권1호
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    • pp.73-81
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    • 2011
  • Six male crossbred (Thai Native${\times}$Anglo Nubian) goats, with an average initial weight of $22{\pm}2\;kg$, were randomly assigned according to a $3{\times}2$ factorial arrangement in a $6{\times}6$ Latin square design with a 21-d period to evaluate the effect of feeding rubber seed kernel (RSK) and palm kernel cake (PKC) in combination on nutrient utilization, rumen fermentation characteristics, and nitrogen utilization. The dietary treatments were as follows: i) concentrate containing 0% RSK and 20% PKC ($T_1$), ii) 0% RSK and 30% PKC ($T_2$), iii) 20% RSK and 20% PKC ($T_3$), iv) 20% RSK and 30% PKC ($T_4$), v) 30% RSK and 20% PKC ($T_5$), and vi) 30% RSK and 30% PKC ($T_6$). During the experiment, signal hay was given on an ad libitum basis as the roughage. It was found that RSK levels and PKC levels had no interaction effects on feed intake, apparent digestibility, $NH_3$-N, blood metabolites, VFA concentrations, and nitrogen utilization, but there were interactions between RSK levels and PKC levels with respect to total DMI (kg/d) and total VFA concentrations, and goats receiving 30% RSK had lower values (p<0.05) than those receiving 0 and 20% RSK, respectively. Feeding different PKC levels did not affect (p>0.05) feed intake, digestibility, rumen fermentation patterns, blood metabolites, and nitrogen utilization. However, increasing RSK levels (>20%) resulted in a slightly lower daily DMI (% BW and g/kg $BW^{0.75}$), apparent digestibility (NDF and ADF), total N intake, and N excretion than in goats fed on 0 and 20% RSK. BUN, blood glucose, and propionate were variable among treatment and were highest in 0% RSK with the 20% PKC fed group having values which were higher than those in other groups. However, there were no differences (p>0.05) among treatments with respect to N retention, PD output, and microbial N supply. Based on this study, RSK levels up to 20% and PKC at 20-30% in concentrate could be efficiently utilized for goats fed on signal hay.

Dental Care Utilization for Examination and Regional Deprivation

  • Kim, Cheol-Sin;Han, Sun-Young;Lee, Seung Eun;Kang, Jeong-Hee;Kim, Chul-Woung
    • Journal of Preventive Medicine and Public Health
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    • 제48권4호
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    • pp.195-202
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    • 2015
  • Objectives: Receiving proper dental care plays a significant role in maintaining good oral health. We investigated the relationship between regional deprivation and dental care utilization. Methods: Multilevel logistic regression was used to identify the relationship between the regional deprivation level and dental care utilization purpose, adjusting for individual-level variables, in adults aged 19+ in the 2008 Korean Community Health Survey (n=220 258). Results: Among Korean adults, 12.8% used dental care to undergo examination and 21.0% visited a dentist for other reasons. In the final model, regional deprivation level was associated with significant variations in dental care utilization for examination (p<0.001). However, this relationship was not shown with dental care utilization for other reasons in the final model. Conclusions: This study's findings suggest that policy interventions should be considered to reduce regional variations in rates of dental care utilization for examination.