• Journal of Plant Biology
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• v.9 no.1_2
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• pp.25-30
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• 1966

The writer presented in the report 7th of this series(Chong, 1959. '62 a.b. '64, '65) the result of fertlizer application on Sasamorpha purpurascens var. borealis with regards to soil conditions, climate, planting position etc. and this report is on the effectiveness of fertilizers by kinds of the new (1-year old) and old(3-to 4 years old) Phyllostachys retticulata. The ineffectiveness of fertilizers for old bamboos aged more than 8-years old has been reported previously. The study site was chosen in a bamboo groven situated at Hyunnae Ri, Okkye Myun, Myungju Gun, Kangwon Do. Each plot was so designed that only one individual remained at the center of a circular area with a dimeter of meters by eliminating the clums and severing the rhizomes, of other individuals around it. And, for the fertilizer test, 10 individuals(the new and old: 5 to 5) were observed per kind of fertilizer used; thus marking the total number of 160 plots in all. The second part of this study was conducted for the potted individuals which numbered 48, successfully established ones out of 55 the transplanted to pots. The 48 were divided into 8 treatments: whole fertilization (NPK＋compost＋human manure); NPK; N; K; Human manure; compost; and the control. Amoung of fertilizers applied was 200 grams for the natural plots and 100 grams for pot; it continued for 3 years and applied twice a year (spring and summer) following the theory of Dr. Koichi-ro Ueda(1954, '60 a.b.). Measurements were taken in August each year. The resutls were confirming the fact that the bamboos require a great deal of fertilizers. The results show the following points: 1) Fertilizers were more effective in the 3 to 4 year old bamboos. 2) The order of effectiveness of fertilizers by kind was the whole fertilization, NPK, and N treatments for Phyllostachys reticulata. 3) Organic matter in addition to commercial fertilizers showed better result than the soil application of the latter due to better soil aeration.

• Clinical and Experimental Reproductive Medicine
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• v.50 no.3
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• pp.177-184
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• 2023

Objective: Reconstructed oocytes after polar body genome transfer constitute a potential therapeutic option for patients with a history of embryo fragmentation and advanced maternal age. However, the rescue of genetic material from the first polar body (PB1) through introduction into the donor cytoplasm is not yet ready for clinical application. Methods: Eighty-five oocytes were obtained following in vitro maturation (IVM) and divided into two groups: PB1 nuclear transfer (PB1NT; n=54) and control (n=31). Following enucleation and PB1 genomic transfer, PB1 fusion was assessed. Subsequently, all fused oocytes underwent intracytoplasmic sperm injection (ICSI) and were cultured in an incubator under a time-lapse monitoring system to evaluate fertilization, embryonic morphokinetic parameters, and cleavage patterns. Results: Following enucleation and fusion, 77.14% of oocytes survived, and 92.59% of polar bodies (PBs) fused. However, the normal fertilization rate was lower in the PB1NT group than in the control group (56.41% vs. 92%, p=0.002). No significant differences were observed in embryo kinetics between the groups, but a significant difference was detected in embryo developmental arrest after the four-cell stage, along with abnormal cleavage division in the PB1NT group. This was followed by significant between-group differences in the implantation potential rate and euploidy status. Most embryos in the PB1NT group had at least one abnormal cleavage division (93.3%, p=0.001). Conclusion: Fresh PB1NT oocytes successfully produced normal zygotes following PB fusion and ICSI in IVM oocytes. However, this was accompanied by low efficiency in developing into cleavage embryos, along with an increase in abnormal cleavage patterns.

• Journal of Korea Foresty Energy
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• v.23 no.1
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• pp.1-18
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• 2004

Effects of nitrogen and phosphorus fertilization on aboveground biomass and distribution of nutrient content in tree components were determined in adjacent 41-year-old plantations of Pinus rigida Miller and Larix kaempferi Gordon on a similar soil in Yangpyeong, Gyeonggi Province. Fertilizer was experimentally applied at the three levels [control(C), 200 N kg/ha+25 P kg/ha(LNP), and 400 N kg/ha+50 P kg/ha(HNP)] in June, 1996. The results obtained from this study were summarized as follows: Total aboveground biomass of P. rigida and L. kaempferi stands was 149, 145ton/ha for control, 166, 149ton/ha for LNP, and 152, 170ton/ha for HNP, respectively, and did not differ between the tree species. Aboveground biomass of P. rigida and L. kaempferi plantations were distributed as follows: stemwood>branch>stembark>foliage. Biomass of understory vegetation was 1,111kg/ha for P. rigida and 907kg/ha for L. kaempferi, and comprised 0.6～0.7% of the total aboveground biomass of the plantations. The concentrations of all nutrients(N, P, K, Ca, Mg) were generally higher in L. kaempferi than in P. rigida because of high nutrient uptake characteristics of Larix spp. Nutrient concentrations in tree components of P. rigida and L. kaempferi plantations were not significantly different among treatments, whereas they were significantly different between species and among tree components. The nutrient concentration in tree components of both tree species decreased in order of foliage>branch>stembark>stemwood. The contents of all nutrients were higher in the fertilized plots than in the control plots, generally the highest in LNP for P. rigida and in HNP for L. kaempferi. Total nutrient(N+P+K+Ca+Mg) contents of aboveground biomass were 703kg/ha for P. rigida and 869kg/ha for L. kaempferi, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.1
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• pp.32-40
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• 2006

The nitrogen (N) absorption and partition of the rice plants are important indicators that can be used to improve the N use efficiency (NUE) of the plants. Improving the plant NUE can help to avoid nutrient waste that may cause environmental pollution. To investigate the N absorption and partition of the rice plants, Hwaseongbyeo (Japonica) and Dasanbyeo (indica/japonica) were applied with N fertilizers at the rates of 60, 120, and 180 kg N per ha in paddy field. Also micro plots of $0.81m^2$ were established inside each plot for application of $^{15}N-labeled$ fertilizer. The differences in N utilization of the rice plants were associated with the total N absorption and partitioning after the heading stage. In the grain filling period, the increase of nitrogen content in the total and leaf blades of Dasanbyeo was higher than that of Hwaseongbyeo. Soil N was the main contributor for the increase of total N of Dasanbyeo during the grain filling period. The N fertilizer uptake rate of Hwaseongbyeo rapidly increased with the increment of N fertilization rate. In Dasanbyeo, N fertilizer uptakes were similar under all rates and times of N application. From heading stage to harvest, Dasanbyeo continued accumulating nitrogen, whereas Hwaseongbyeo had small changes. In conclusion, the difference in nitrogen absorption and partition after heading of the two cultivars was caused by the ability of Dasanbyeo to accumulate and remobilize soil nitrogen to the grains during the grain filling period.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.102-109
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• 1990

This experiment was carried out to study the effect of pure mineral nitrogen fertilizing on dry matter yield of grassland and the advisable mineral nitrogen amounts in long duration under practical conditions at the "Federal Institute for Agriculture in the Alps" in Austria. The application rates were 0, 30, 60,90 and 120 kg N/ha/cut, the cutting regimes 3-, 4-, 5- and 6-cuts/year. In order to explain the nitrogen-profitability were determined that 1 kg pure mineral nitrogen have to produce 8 - 16 kg DM/kg N in dependence on cutting regimes and requiring of nitrogen efficiencies. The results were as follows: 1. With only PK-fertilizaing average dry matter yields from 4.0 to 7.6 tons per ha and year were obtained. 2. Within all applied cutting regimes 60 kg min. Nlhalgrowth have proved to be the most efficient application rate witn 13 - 24 kg DM/kg N in dependent of cutting regimes. Comapred with only PK-treatment the DM yields increased by 3.9 - 4.7 t/ ha nad year. 3. By the sigmaformed process of Input-Output curve the highest marginal yield (the "most efficient" Ndressing rate) per ha and year was calculated: 152 kg N at 3-cut regimes, 204 kg N at 4-cut regimes, 220 kg N at 5-cut regimes and 240 kg N/ha/year at 6-cut regimes. 4. With required efficiencies of 16 and 12 kg DM/kg N 240 - 300 kg N per ha and year respectively would have to be applied at 3-cut regimes; with required efficiencies of 12 and 10 kg DM/kg N at 4-cut regimes the appropriate figures ranged from 320 to 420 kg N/ha and year, at 5- and 6-cut regimes and efficiencies of 10 and 8 kg DM/kg N results of 360 - 460 kg N and 380 - 500 kg N respectively were obtained. 5. At the relatively dry location Piber the highest dressing rates were needed in order to obtain the efficiencies from 8 to 16 kg DM/kg N, about 30 - 60 kg N/ha/year more than at the relatively moist location Admont.ist location Admont.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.598-603
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• 2014

The level of nitrous oxide ($N_2O$), a long-lived greenhouse gas, in atmosphere has increased mainly due to anthropogenic sources, especially application of nitrogen fertilizers. Quantifying $N_2O$ emission in the agricultural field is essential to develop National inventories of greenhouse gases (GHGs) emission. The objective of this study was to develop emission factor to estimate direct $N_2O$ emission from agricultural field by measuring $N_2O$ emissions in the red pepper cultivating field from 2010 to 2012. Emission factor of $N_2O$ calculated from accumulated $N_2O$ emission, nitrogen fertilization rate, and background $N_2O$ emission was $0.0086{\pm}0.00043kg$ $N_2O-N\;kg^{-1}$ N resulted from three year experiment of the research sites. More extensive studies need to be conducted to develop $N_2O$ emission factors for other upland crops in the various regions of Korea because $N_2O$ emission is influenced by many factors including climate characteristics, soil properties, and agricultural practices.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.6
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• pp.751-760
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• 2015

Management of renewable organic resources is important in attaining the sustainability of agricultural production. However, nutrient management with organic resources is more complex than fertilization with chemical fertilizer because the composition of the organic input or the environmental condition will influence organic matter decomposition and nutrient release. One of the most effective methods for estimating nutrient release from organic amendment is the use of N mineralization models. The present study aimed at parameterizing N mineralization models for a number of organic amendments being used as a nutrient source for crop production. Laboratory incubation experiment was conducted in aerobic condition. N mineralization was investigated for nineteen organic amendments in sandy soil and clay soil at $20^{\circ}C$, $25^{\circ}C$, and $30^{\circ}C$. N mineralization was facilitated at higher temperature condition. Negative correlation was observed between mineralized N and C:N ratio of organic amendments. N mineralization process was slower in clay soil than in sandy soil and this was mainly due to the delayed nitrification. The single and the double exponential models were used to estimate N mineralization of the organic amendments. N mineralization potential $N_p$ and mineralization rate k were estimated in different temperature and soil conditions. Estimated $N_p$ ranged from 28.8 to 228.1 and k from 0.0066 to 0.6932. The double exponential model showed better prediction of N mineralization compared with the single exponential model, particularly for organic amendments with high C:N ratio. It is expected that the model parameters estimated based on the incubation experiment could be used to design nutrient management planning in environment-friendly agriculture.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.2
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• pp.150-157
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• 1996

This study was conducted to observe the heavy matal contents of paddy soil and brown rice under long term fertilization with different soil amelioration materials. The contents of 0.1N HCl extractable Cu and Zn of surface soil increased in the plots of long term application with urea, ammonium sulfate, compost, lime and silicate fertilizers. The Cu and Zn contents of brown rice showed decreasing trends in same treatments of fertilizers and soil amelioration materials. But the contents of Cd and Pb in soil and brown rice were not affected in long term fertilization with soil amelioration materials.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.8
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• pp.1127-1133
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• 2008

This study was designed to develop a method of liquid storage of boar sperm at $4^{\circ}C$ by using the modified Beltsville F5 (BF5) diluent with bovine serum albumin (BSA) and N-acetyl-D-glucosamine. Boar sperm were stored in lactose-egg yolk and N-acetyl-D-glucosamine (LEN), BF5 and Golden-Pig liquid 4 (GPL4) diluents at $4^{\circ}C$ for 5 days and were examined for sperm viability, adenosine triphosphate (ATP) and in vitro fertilization (IVF). The percentage of sperm viability in GPL4 diluent was higher than in LEN and BF5 diluent from 1 to 5 days of storage at $4^{\circ}C$. The percentage of sperm viability steadily declined from 1 to 5 days of storage in the three different diluents. Sperm ATP in GPL4 diluent was higher than in LEN and BF5 diluents from 1 to 5 days of storage. Sperm ATP rapidly declined after 5 days of storage in the three different diluents. Porcine oocytes matured in vitro were inseminated with different sperm concentrations of liquid semen stored for 3 days in GPL4 diluent. The percentage of monospermic oocytes did not show any differences from 2.5 to $20{\times}10^5$ sperm/ml. However, the percentage of polyspermic oocytes in the sperm concentration of $2.5{\times}10^5$ sperm/ml was lower than in concentrations of 5, 10 and $20{\times}10^5$5 sperm/ml. The percentage of blastocysts from the cleaved oocytes at $2.5{\times}10^5/ml$ sperm concentration was significantly lower than at 5, 10 and $20{\times}10^5sperm/ml$ concentrations. In conclusion, GPL4 diluent can be stored at $4^{\circ}C$ for 5 days and showed higher sperm viability and sperm ATP concentration compared with LEN and BF5 diluents. Also, we found that GPL4 diluent can be used for IVF of porcine oocytes.

• Clinical and Experimental Reproductive Medicine
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• v.42 no.4
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• pp.163-168
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• 2015

Objective: To investigate the impact of fibroids on the blood flow of the uterine and subendometrial arteries and in vitro fertilization (IVF) outcomes. Methods: In this study, we analyzed 86 IVF/intracytoplasmic sperm injection (ICSI) cycles in which a gonadotropin-releasing hormone antagonist protocol was used for controlled ovarian stimulation between January 2008 and March 2009. The subjects comprised 86 infertile women with (fibroid group, n=43) or without (control group, n=43) uterine fibroids. Results: Patient characteristics were similar between the fibroid and control groups. The IVF/ICSI outcomes in patients with fibroids were similar to those of patients in the control group. The resistance index (RI) and pulsatile index (PI) of the uterine and subendometrial arteries on the day of embryo transfer were also comparable between the two groups. IVF outcomes and uterine hemodynamics in patients with multiple (${\geq}2$) fibroids were similar to those of patients with a single fibroid. However, clinical pregnancy and implantation rates were significantly lower in patients with fibroids who experienced uterine cavity distortion than in patients with fibroids who had a normal uterine cavity (both p<0.05). The RI and PI of the subendometrial artery were significantly higher on the day of embryo transfer in patients with fibroids who experienced uterine cavity distortion than in patients with fibroids who had a normal uterine cavity (both p<0.05). Conclusion: Fibroids which distorting the uterine cavity might impair the subendometrial artery blood flow clinical pregnancy rate and embryo implantation rate in infertile patients undergoing IVF. Otherwise, IVF outcomes were not influenced by the presence of uterine fibroids.