• 한국가금학회지
• /
• 제15권2호
• /
• pp.67-71
• /
• 1988

백색 Leghorn 암탉의 난자생성과정 중 감수분열을 억제하여 2배수성 난자(2n)의 생산에 근거한 다배교성 가축생산과 능력개양의 기초연구인 본 실험에서 감수분열시기는 배난 전 2~4시간이고 이때에 체중 kg당 0.3mg의 TEM(Tri ethylene melamin)을 복강에 주입하여 수정율 38%의 계란을 얻었고 이들 중 66%가 TEM의 영향으로 2n 의 난자가 생성되어 정상정자와의 결합으로 3배수성(3n)의 배아로 나타났다.

• 한국토양비료학회지
• /
• 제7권1호
• /
• pp.35-42
• /
• 1974

기존장려품종 IR 667에 대(對)하여 전국적(全國的)으로 실시한 2년간의 3요소(三要素) 요인시험(要因試驗) 결과로부터 품종간생산력(品種間生産力), 토양생산력(土壤生産力)(지력(地力))과 시비생산력(施肥生産力)(시비(施肥)에 의(依)한 증수량(增水量)을 검토(檢討)하였다. 1. IR 667은 1971년에 19%, 저온장해(低溫障害)가 있었던 72년엔 12%의 증수를 보였으며 이는 수당입수(穗當粒數)가 큰데 기인(基因)하였다. 수량(收量)의 변이계수(變異係數)는 IR667이 적었으나 장려품종이 23% (1971)와 30% (1972)의 포장에서 수량(收量)이 높았다. 2. IR 667은 질소(窒素) 특히 인산(燐酸)과 가리(加里)를 더 많이 요구(要求)하나 시비효율(施肥效率)(증수량(增收量)/시비량(施肥量))은 질소(窒素)에서만 약(約) 4kg 높고 인산(燐酸)은 장려품종보다 떨어지며 가리(加里)는 차이가 없었다. 3. IR 667에서 토양생산력(土壤生産力)은 크지만 토양생산력(土壤生産力)의 최대수량(最大收量)에의 기여도(寄與度)는 적었다. 4. 시비생산력(施肥生産力)의 기여도(寄與度)는 19~33%이며 이중 약(約) 5%만이 인산(燐酸)과 가리(加里)의 기여도(寄與度)였다. 5. 토양생산력(土壤生産力)이 커질수록 포장생산력(圃場生産力)이 커지나 시비생산력(施肥生産力)은 감소하여 지력(地力)의 기여도(寄與度)가 증가(增加)한다. 6. 시비효율(施肥效率)이 개무(皆無)인 비옥도(肥沃度)을 포화점(飽和點) 설정(設定)하고 이를 토양생산력(土壤生産力)(또는 포장생산력(圃場生産力))의 상한지표(上限指標)로 볼 수 있었다.

• 한국가축번식학회지
• /
• 제23권3호
• /
• pp.221-227
• /
• 1999

미성숙한 소 난자 동결보존 기술의 개발은 체외수정, 복제동물 및 형질전환동물 생산에 필요로 하는 난자를 시간과 공간의 제약 없이 공급이 가능해지기 때문에 그 효용가치가 많으나 아직까지 성공 보고례가 없다. 본 연구에서는 전자현미경용 grid를 이용한 초급속 동결 방법에 의해 미성숙한 소 난자를 동결 보존한 후, 이 난자를 융해하여 체외성숙, 체외수정 및 배발달을 유도하였다. 동해제는 PBS에 40% ethylene glycol, 0.5M sucrose, 18%과 10% fetal bovine serum이 들어 있는 EFS40을 사용하였다 . 동결ㆍ융해 후의 난자의 생존율은 48% 정도로 대조군에 비해 현저히 낮았으나 metaphase-II까지의 성숙율은 78%로, 정상 자웅전핵 형성율 75%로 대조군에 비해 차이가 없었다. 또한 배반포까지의 배발달율은 대조군23%와 동결군은 5%로 동결 융해한 것이 낮았으며 수정후 108~120시간 째 배반포를 염색하여 세포수률 알아본 결과는 각각 128$\pm$5와 98$\pm$8이었다. 항동해제내 $Na^{＋}$ 이온의 농도에 따른 미성숙된 난자의 생존율, 성숙, 수정 및 배발달율을 조사하였으나 유의차가 없었다. 이상의 결과는 동결 보존된 미성숙 소 난자가 융해후 체외성숙 및 수정에 의해 배반포까지의 발달이 가능하다는 것을 시사하고 있다.

• 한국동물번식학회:학술대회논문집
• /
• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
• /
• pp.63-63
• /
• 2003

This study was carried out to investigate the effects of liquid boar sperm stored at 4$^{\circ}C$ on sperm motility, normal acrosome, and in-vitro fertilization and culture of pig oocytes matured in-vitro. The sperm-rich fraction (30~60 ml) of ejaculate was collected into an insulated vacuum bottle. Semen was slowly cooled to room temperature (20~23$^{\circ}C$) by 2 h after collection. Semen was transferred into 15 ml tubes, centrifuged at room temperature for 10 min at 800$\times$g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5 ml of lactose, egg yolk and N-acetyl-D-glucosamine (LEN) diluent to provide 1.0$\times$10$^{9}$ sperm/ml at room temperature. The resuspended semen was cooled in a refrigerator to 4$^{\circ}C$ and preserved for 5 days to examine sperm motility and normal acrosome. The medium used for oocyte maturation was modified tissue culture medium (TCM) 199. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at 38.5$^{\circ}C$, 5% $CO_2$ in air. Oocytes were inseminated with liquid boar sperm stored at 4$^{\circ}C$ for 2 days after collection. Oocytes were coincubated for 6 h in 500 ${mu}ell$ mTBM fertilization media with 0.2, 1, 5 and 10$\times$10$^{6}$ /ml sperm concentration, respectively. At 6 h after IVF, oocytes were transferred into 500 ${mu}ell$ Hepes-buffered NCSU-23 culture medium for further culture of 6, 48 and 144 h. There were significant differences in sperm motility and normal acrosome among preservation days and incubation times, respectively. The rates of sperm penetration and polyspermy were higher in 5 and 10$\times$10$^{6}$ sperm/ml than in 0.2 and 1$\times$10$^{6}$ sperm/ml. Male pronuclear formation was lower in 0.2$\times$10$^{6}$ sperm/ml than in 1, 5 and 10$\times$10$^{6}$ sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in 10$\times$10$^{6}$ sperm/ml compared with other sperm concentrations. The rate of blastocysts from the cleaved oocytes (2~4 cell stage) was highest in 1$\times$10$^{6}$ sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at 4$^{\circ}C$ could be used for in-vitro fertilization of pig oocytes matured in-vitro. Also, we recommend 1$\times$10$^{6}$ ml sperm concentration for in-vitro fertilization of pig oocytes.

• 한국환경농학회지
• /
• 제42권1호
• /
• pp.44-51
• /
• 2023

Nitrogen fertilizer is an essential macronutrient that requires repeated input for crop cultivation. Excessive use of nitrogen fertilizers can adversely affect the environment by discharging NH₃, NO, and N₂O into the air and leaching into surrounding water systems through rainfall runoff. Therefore, it is necessary to develop a technology that reduces the amount of nitrogen fertilizer used without compromising crop yields. Fertilizer deep placement could be a technology employed to increase the efficiency of nitrogen fertilizer use. In this study, a deep fertilization device that can be coupled to a tractor and used to inject fertilizer into the soil was developed. The deep fertilization device consisted of a tractor attachment part, fertilizer amount control and supply part, and an underground fertilizer input part. The fertilization depth was designed to be adjustable from the soil surface down to a depth of 40 cm in the soil. This device injected fertilizer at a speed of 2,000 m²/hr to a depth of 25 to 30 cm through an underground fertilizer injection pipe while being attached to and towed by a 62-horsepower agricultural tractor. Furthermore, it had no difficulty in employing various fertilizers currently utilized in agricultural fields, and it operated well. It could also perform fertilization and plowing work, thereby further simplifying agricultural labor. In this study, a newly developed device was used to investigate the effects of deep fertilizer placement (FDP) compared to those with urea surface broadcasting, in terms of rice and soybean grain yields. FDP increased the number of rice grains, resulting in an average improvement of 9% in rice yields across three regions. It also increased the number of soybean pods, resulting in an average increase of 23% in soybean yields across the three regions. The results of this study suggest that the newly developed deep fertilization device can efficiently and rapidly inject fertilizer into the soil at depths of 25 to 30 cm. This fertilizer deep placement strategy will be an effective fertilizer application method used to increase rice and soybean yields, in addition to reducing nitrogen fertilizer use, under conventional rice and soybean cultivation conditions.

• 한국토양비료학회지
• /
• 제46권6호
• /
• pp.652-658
• /
• 2013

A huge number of greenhouse soils in Korea have accumulated mineral elements which induce many nutritional and pathological problems. The present study was performed to the effects of the reduced fertilization on plant growth, and uptake and partitioning of minerals (N, P, K) and soluble carbohydrates using highly minerals-accumulated farmer's greenhouse soil. On the basis of the recommended application for tomato crop, the application rates of N, P and K were 110(50%)-5.2(5%)-41.5(35%)kg $ha^{-1}$, respectively, using Hoagland's nutrient solution. Tomato growth rates during the whole experiment were not significant between treatments, but it was found that a decrease in daily growth represented after 60 days of treatment (DAT). The reduced application led to a drastic decrease in the concentration of N, P and K in fruits, and, thus, this resulted in lower uptake after 40 DAT. The lower phloem export and utilization of soluble carbohydrates caused an accumulation of extra-carbohydrates in leaves, stems and fruits in the reduced application. The reduced fertilization induced the capture of N, P and K in leaves and of soluble carbohydrates in stems compared to the conventional application. In this study, we suggest that it is possible to delay the first fertigation time in minerals-accumulated soils without an adverse impact on crop growth, but it is necessary to regularly monitor mineral status in soil to ensure a balanced uptake, synthesis and partitioning of minerals and carbohydrates.

• Clinical and Experimental Reproductive Medicine
• /
• 제44권2호
• /
• pp.79-84
• /
• 2017

Objective: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate the effects of different media on the IVM outcomes of immature oocytes at the germinal vesicle (GV) stage. Methods: A total of 400 immature oocytes at the GV stage with normal morphology were retrieved from 320 infertile women aged $31{\pm}4.63years$ during stimulated intracytoplasmic sperm injection (ICSI) cycles. They were divided into groups of homemade IVM medium (I, n = 100), cleavage medium (II, n = 100), blastocyst medium (III, n = 100), and Sage IVM medium (IV, n = 100) and cultured for 24 to 48 hours at $37^{\circ}C$. ICSI was performed, and the rates of fertilization and embryo formation were compared across the four groups. Results: In the 400 retrieved GV oocytes, the total maturation rates showed significant differences in groups I to IV (55%, 53%, 78%, and 68%, respectively, p<0.001). However, there were no significant differences in the fertilization, embryo formation, or arrest rates of metaphase II oocytes across these groups. In all groups, GV maturation was mostly completed after 24 hours, with fewer oocytes requiring 48 hours to mature (p<0.01). Moreover, the rate of high-quality embryos was higher in group IV than in the other groups (p=0.01). Conclusion: The quality of the IVM medium was found to affect clinical IVM outcomes. Additionally, blastocyst medium may be a good choice in IVM/ICSI cycles as an alternative IVM medium.

• 한국작물학회지
• /
• 제52권3호
• /
• pp.296-302
• /
• 2007

This study was conducted to evaluate the effect of slow release fertilizers (SRF), crotonylidene diurea (CDU) and latex coated urea (LCU), on nitrogen (N) use efficiency (NUE) and nitrate-N leaching in a silty clay loam soil under polyethylene film mulching (PFM) for sesame cultivation. In PFM plot, concentrations of $NO_3-N$ and $NH_4-N$ in SRF applied soils were less than that in the urea plot during the whole growing period. However, $NO_3-N$ and $NH_4-N$ in all the non-mulched plots (NM) were not significantly different. Urea-N in soil treated with SRF was higher than urea plot until 50 days after application and was comparable in all the treatments after 50 days. $NO_3-N$ concentrations in leached solution in 21 days after urea fertilization in PFM and NM were 26 mg $L^{-1}$ and 83 mg $L^{-1}$, respectively. However, $NO_3-N$ in leached solution at applied CDU and LCU was less than that of urea similar to nitrate concentration in soil. $NO_3-N$ in leached solution in applied CDU and LCU in 44 days after application was about 25% lower than that urea plot and PFM, while the $NO_3-N$ concentration of CDU and LCU treatment in NM did not changed. Application of SRF increased the yield of sesame and N recovery compared to urea and there was a little difference between SRF and N levels. In conclusion, application of 80% N level with SRF increased N recovery and reduced nitrate leaching without reduction of yields compared with urea application.

• 한국토양비료학회지
• /
• 제40권1호
• /
• pp.77-82
• /
• 2007

질소비료로부터 암모니아의 휘산은 자연적으로 존재하는 모든 토양에서 일어나는 질소 손실의 주된 기작이다. 암모니아 휘산은 다양한 토양과 환경의 조건 및 비료관리 방안에 의해 영향을 받는다. 질소비료 의존도가 높은 채소들도 휘산된 암모니아 가스에 의해 피해를 받는 사례가 종종 보고되고 있다. 본 연구에서는 표토에 시용된 요소비료로부터 암모니아 휘산량을 측정하였고, 이에 미치는 요소비료 시용량, 관개시기, 및 온도 등의 비료관리요인들의 영향을 조사했다. 암모니아 휘산은 요소를 시용한 뒤 약 3일 후에 시작되었으며, 약 2주 후에 최대에 도달하였다. 17일 후, 휘산된 암모니아태 질소의 양은 200, 400, $600kg\;N\;ha^{-1}$ 의 시용량에서 각각 3.0, 4.4, 그리고 8.0 kg 이었다. 이들 휘산량은 시용된 질소가 15.0, 10.9, 및 13.0% 가 손실된 것과 상응한다. 온도가 5, 8, 22, $28^{\circ}C$ 일때 휘산된 질소의 양은 각각 5, 21, 75, $87kg\;N\;ha^{-1}$ 이였다. 요소비료를 시용한 뒤 0, 5, 10 mm의 물을 관개한 경우, 휘산된 질소의 양은 각각 21.3, 21.2, $16.6kg\;N\;ha^{-1}$ 이었다. 한편, 요소를 시용한 후 5 mm를 관수한 경우의 질소 휘산량은 $10.44kg\;N\;ha^{-1}$ 로 감소하였다. 그러므로 요소비료를 권장량을 표토와 혼합, 온도가 낮을 때 그리고 요소비료를 시용후 즉시 관개하는 방안이 암모니아 휘산에 의한 질소 손실을 최소화 하는 비료관리 방안이었다.

• 한국토양비료학회지
• /
• 제32권2호
• /
• pp.109-114
• /
• 1999

벼 집약적(集約的) 재배증계하(栽培證系下)에서 화학비료 시용량 절감 및 퇴비의 이용가능성(利用可能性)을 검토키 위하여 영남농업시험장에서 30년간 실시한 동일비료(同一肥料) 장기연용(長期連用)시험을 중심으로 벼 수량성 및 질소이용효율의 연도별 변이를 분석한 결과는 다음과 같다. 동일비료(同一肥料)연용년도별 벼 수량성(收量性)변화는 퇴비단용구에서 통계적으로 유의한 증가를 보인 반면 무비구와 질소결제구에서는 감소의 경향을 보였고, 퇴비(堆肥)의 효과(效果)는 초기(初期) 10년간(年間)은 뚜렷한 차(差)가 없었으나 그 후(後) 점증(漸增)하여 30년(年)이 경과(經過)한 1996년(年)의 수량(收量)은 3요소구(要素區)의 80%수준(水準)이었다. 처리별(處理別) 요소흡수량(窒素吸收量)은 3요소+퇴비구가 $167kg\;ha^{-1}$로 높았으며 질소회수율(窒素回收率)($RE_N$)은 퇴비단용시(堆肥單用時) 0.74로 가장 높은 반면, 인산(燐酸) 및 칼리결제구(缺除區)가 0.30낮았다. 3요소(要素) 시용구(施用區)에서 질소(窒素)의 농업적(農業的) 효율(效率)($AE_N$)은 평균 12.8 kg/kg N인 반면, 질소비료생산성(窒素肥料生産性)($PFP_N$)은 37.7 kg/kg N으로 높았으며 AE_N과 PFP_N 공히 시험초대에는 감소하는 경향을 보였으나 후기 연대에는 $AE-N$의 경우 증감이 없는 반면, $PFP_N$은 증가하였다. $AE_N$와 $PFP_N$ 차(差)를 천연질소공급력(天然窒素供給力)(INS)으로 표시할 수 있으므로 그 차는 3요소(要素)+퇴비구(堆肥區)가 28.4로 3요소구(要素區)에 비해 높았으며 시험년수(試驗年數)가 진행됨에 따라 INS는 더욱 커졌다.