• Title/Summary/Keyword: Mycosporine-like amino acids (MAAs)

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Advancement of Mass Purification of Porphyra 334 from Porphyra yezoensis by Applying Direct Current (직류전류 인가를 통한 김으로부터 포피라334의 대량 정제 고도화)

  • Jang, Sung Joo;Lee, Jeong Hun;Seo, Hyo Hyun;Lee, Sak;Kim, Jiyeon;Kim, Doyoun;Moh, Sang Hyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.12 no.1
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    • pp.66-74
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    • 2020
  • Marine algae contain a variety of substances, such as mycosporine-like amino acids, which can defend against UV irradiation. Among them, Porphyra-334 derived from Porphyra yezoensis is attracting attention as a novel active ingredient for anti-aging cosmetics because of its excellent anti-oxidative, anti-inflammatory, and wound-healing properties through promoting skin cell migration. In this study, a process using direct current (DC) for increasing the yield of large-scale purification of Porphyra-334 was developed. When DC was applied to obtain Porphyra-334 efficiently, the purification time was shortened by approximately 1/4 compared with the process wherein DC was not applied; moreover, the yield of purification was improved.

Role of Shinorine derived from Microalgae in skin protection (미세조류 유래 Shinorine의 피부세포에서의 효능)

  • Jung, Hae Soo;Cho, Moon Jin;Seo, Hyo Hyun;Kulkarni, Atul;Suh, Seung Suk;Lee, Taek Kyun;Moh, Sang Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.7
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    • pp.4416-4422
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    • 2014
  • In the present study, mycosporine-like amino acids (MAAs)were extracted from in Chlamydomonas hedleyi, and their function was investigated regarding to protective capacity against UV radiation and a possibility to be developed into functional suncream including MAAs by using natural compounds. In particulr, we assessed UV protective ability and anti-inflammation of shinorine in human skin cells. As a result, shinorine can protect the skin against damage by the absorption of energy from UV radiation and functions as an anti-wrinkling substrate through a increase of collagen synthesis. These data suggest that shinorine can be utilized not only as a substrate to protect UV radiation, but anti-aging material in cosmetic products.

Influence of UV-B Radiation on Photosynthesis, Growth and Pigmentation of Chondrus ocellatus (Rhodophyta) from Shallow Water

  • Taejun Han;Han, Young-Seok;Cho, Man-Gee;Park, Jin-Hee;Goo, Jae-Gun;Kang, Sung-Ho
    • Korean Journal of Environmental Biology
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    • v.21 no.4
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    • pp.368-376
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    • 2003
  • The UV-B sensitivity was tested for the intertidal species Chondrus ocellatus from Korea, by measuring photosynthesis estimated as effective quantum yield ($\Phi_{PSII}$) of photosystem II (PS II), growth and content and composition of photosynthetic pigments and UV-absorbing pigments (UVAPs). The $$\Phi_{PSII}$ of the alga decreased with increasing time of exposure to UV-B radiation, followed by fast and nearly full recovery indicating dynamic photoinhibiton. Fresh weight-based growth and pigment contents of C. ocellatus were not seriously affected by UV-B radiation. A single broad peak at 327 nm was obtained from methanol extracts of C. ocellatus, and the absorbance peak increased with increasing UV. The single peak was resolved into three peaks (311, 330 and 336 nm) by the fourth -derivative, and quantitative change in response to UV-B radiation occurred only at 330 nm. High performance liquid chromatography (HPLC) analysis of purified extracts indicated that three MAAs (mycosporine-like amino acids) are present, asterina 330, palythine and shinorine. Field observations during three growing months showed that C. ocellatus exhibit the highest amount of UVAPs in May followed by July and little trace in September, coinciding with the species' phenology. In an ecological context, dynamic photoinhibition as well as accumulation of UVAPs may enable the shallow water red alga C. ocellatus to be well adapted to high UV-B environments.