• Journal of Forest and Environmental Science
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• v.32 no.2
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• pp.158-163
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• 2016

Mushroom surveys and collections were conducted in the western and eastern forest areas in Cambodia, and then fungal biodiversity was analyzed by identifying mushrooms. One thousand and three hundreds eighty three specimens were identified by morphological and genetical characteristics, and were classified into 238 species, 160 genera, 52 families, 15 orders, and 3 phylums. The collected mushrooms were immersed in 70% ethyl alcohol for DNA extraction, and the rest of them were dried in the portable mushroom dryer for 12 hrs. Among these mushrooms, the genera Mycena (8.7%), Ganoderma (5.6%), Microporus (5.3%), Marasmius (4.2%), Marasmiellus (3.0%), Phellinus (2.5%), Trametes (2.5%), Hygrocybe (1.9%) and Pycnoporus (1.5%) were dominant. In the western Cambodia, 1,061 specimens were collected from Koh Kong forests, while 263 specimens were collected from the eastern Cambodia, Seima and Mondulkiri forests. Elevations of surveyed sites were ranged from 0 to 750 m above sea level. The number of species observed in the elevation of 251-500 m was the highest as compared to the other ranges of elevation. Daldinia concentrica, Microporus vernicipes, Microporus xanthopus, Pycnoporus coccineus, Stereum hirsutum, and Stereum ostrea were commonly distributed in all ranges of elevation, while the distribution of Ceratomyxa fruticulosa, Panus fulvus, Schizophyllum, Trametes versicolor, and Tyromyces chioneus were limited under 500 m. One hundred and forty one species including Amauroderma sp., Bjerkandera adusta, Trichaptum abietinum, and Tyromyces chioneus were collected only in Cardamom, while 20 species including Auricularia auricula-judae, Coriolopsis sanguinaria, Rigidoporus microporus, and Xylaria polymorpha were collected only in Seima. Ganoderma sp., Mycena sp., Marasmius sp., Microporus xanthopus, Phellinus sp., and Russula sp. were dominant species in both the western and eastern Cambodia. Species diversity indices in the eastern and western survey sites were 1.83 and 1.77, respectively, while evenness indices were 0.92 and 0.90. The species similarity index between two survey sites was 0.42.

• Journal of Mushroom
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• v.3 no.4
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• pp.145-153
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• 2005

Two hundred seventy-four microorganisms were isolated from the soil of abandoned mines around for selecting the heavy metal-degrading strains and their microbial diversity was analyzed. The specimens of macrofungi were isolated from the soil of abandoned mines around for selecting the heavy metal-degrading strains. Eunseong and Dogok in Gyeongsangbuk-do, for selecting the heavy metal-degrading strains. Soil was also collected from same location. Contents of heavy metals (Cd, Cu, Pb, Zn, Ni, Cr, and As) were determined spectrometically in fruiting bodies of forty eight wild macrofungi specimens with soils. When the analysed their transfer factor from soil to fruiting body, Amanita volvata have the highest transfer factor of cadmium and arsenic. And Mycena pura showed the highest in mercury, Marasmius pulcherripes in zinc, Laccaria laccata in nickel, and Collybia confluens in chrome. When compare the population of mushrooms between the contaminated mines and Mt. Chiak as the healthy area, Genus Russula and Collybia were collected both area, but Leucocoprinus, Coprinus, Suillus, Lepiost, Gyroporus, Lepista, Microstoma, Stropharia, and Agrocybe were only in the contaminated mine area.

• Mycobiology
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• v.48 no.3
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• pp.157-168
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• 2020

The Mount Juwang was designated as a national park in 1976 because of its unique bedrock geology. Although Juwang National Park has maintained its natural ecosystem well, few macrofungal surveys have been carried out. As a part of a project supported by the National Institute of Biological Resources (NIBR) for the discovery of indigenous fungal species, we surveyed the mushrooms in Juwang National Park from 2018 to 2019. The macrofungi were identified based on morphological and molecular analyses. Among these fungi, five specimens were identified as species previously unrecorded in Korea: Calocybe decolorata, Crepidotus brunnescens, Mycena pearsoniana, Psathyrella phegophila, and P. sulcatotuberculosa. Three of these species are known to be rare in the world: Crepidotus brunnescens, P. phegophila, and P. sulcatotuberculosa. In this study, we provide detailed morphological descriptions of the five unrecorded species from Mt. Juwang in Korea.

• Mycobiology
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• v.45 no.4
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• pp.286-296
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• 2017

Ulleung Island is a biodiversity hotspot in South Korea. During a survey of indigenous fungal species from Ulleung Island conducted from 2015 to 2016, we discovered 10 unrecorded macrofungi in Korea. These macrofungi were identified to the species level using morphological features and phylogenetic analysis based on the internal transcribed spacer region: Deconica phyllogena, Mycena zephirus, Phaeomarasmius proximans, Phlebia radiata, Pluteus semibulbosus, Postia alni, Resinicium pinicola, Scytinostroma portentosum, Tricholomopsis flammula, and Tyromyces kmetii. We also provide detailed morphological descriptions for these 10 species.

• Journal of Microbiology and Biotechnology
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• v.23 no.10
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• pp.1347-1356
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• 2013

Tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of Pinus denisflora, forms a fruiting body that serves as a valuable food in Asia. However, the artificial culture of this fungus has not been successful. Soil fungi, including T. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of T. matsutake. Here, we report on the structures of fungal communities associated with the fairy ring of T. matsutake through the pyrosequencing method. Soil samples were collected inside the fairy ring zone, in the fairy ring zone, and outside the fairy ring zone. A total of 37,125 sequencing reads were obtained and 728 to 1,962 operational taxonomic units (OTUs) were observed in the sampling zones. The fairy ring zone had the lowest OTUs and the lowest fungal diversity of all sampling zones. The number of OTUs and fungal taxa inside and outside the fairy ring zone was, respectively, about 2 times and 1.5 times higher than the fairy ring. Taxonomic analysis showed that each sampling zone has different fungal communities. In particular, out of 209 genera total, 6 genera in the fairy ring zone, such as Hemimycena, were uniquely present and 31 genera, such as Mycena, Boletopsis, and Repetophragma, were specifically absent. The results of metagenomic analysis based on the pyrosequencing indicate a decrease of fungal communities in the fairy ring zone and changes of fungal communities depending on the fairy ring growth of T. matsutake.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.19-19
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• 2014

This study included the analysis of mushroom data collected from Mt. Chiak in Gangwon-do using various methods. Former studies of Korean mushrooms are limited by regional characters and there is less species diversity among the regions. This study tried to find a way for the forecast of mushroom distribution and appearance by indexes of species diversity. The indexes used in this study include the number of fungi (N), the number of species (S), similarity index (C), richness index (R1, R2), variety index (V1, V2), evenness index (E1, E2, E3, E4, E5), and dominance index (D1) to analyze variety of species diversity. Analyses of data of fungi using a multistage cluster sampling indicate that the average value of C for years was higher than the average value of C for areas. The mushrooms consisted of 208 species in 686 individuals in limited fungal collection from 2002 to 2003. One hundred thirty nine species in 393 individuals were collected in 2002, and 122 species 293 individuals were collected in 2003. The individuals collected in 2003 were smaller than 2002's individuals. Similarity, richness, and variety indexes' values of 2003 were reduced than 2002's values but dominance index of 2003 was increased than 2002's value. Generally the species diversity of the environment to evaluate the index of similarity, richness, and variety was a higher index; dominance index was lower than that of the surrounding environment, suggesting a good diversity. As a result, the occurrence of mushrooms in the surrounding environment and the various factors seem fell in 2002 compared to 2003. The majority genus of the limited fungal collection was Mycena genus in 63 individuals; the majority species was Laccaria laccata in 34 individuals. Ninety three species in 106 individuals were collected by the extended collection and the majority genus of the extended collection was Amanita genus in 17 individuals; the majority species was Amanita citrina (Schaeff.) Pers. which was found in 5 individuals. This demonstrates that periodical similarity's value was 0.159 is higher than special similarity's 0.119. This indicates that the probability of the appearance of same mushrooms in the same area in following year is higher than the probability of the appearance of same mushrooms in the surrounding area in same year. The value of coefficient of variation (CV), in which the amount of change is much or less by N is higher than the CV value by S. CV value of dominance index(D) was the highest r point among other indexes, and evenness index (E) was the lowest point among other indexes. The correlation matrix with 66 combinations between the indexes, the combinations with correlations was 46 combinations. These results revealed that indexes of R1, V2, and E1 were proper to represent species diversity of fungi based on the correlation matrix and the theory of statistical independence which means there is no or less mutual association. This research would contribute to the study about variable living creature by measuring method and in the future this would be used to figure out regulation about fungi with their correlation, values in ecosystem, develop improving new models about agricultural fungi species and numbers by investigating agricultural variable species.