• The Korean Journal of Mycology
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• v.9 no.1
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• pp.31-37
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• 1981

Mycelial growth and fruit body formation of Diehlomyces microsporus were best on mushroom spawn extract medium and rice bran extract medium, respectively. L-asparagine, fructose and glucose were good nutrient sources for mycelial growth. Optimum temperature for mycelial growth ranged at $25{\sim}28^{\circ}C$. Maximum mycelial growth occurred at pH 5.5 while optimum pH for ascospore germination was 6.0. Mycelial mats of D. microsporus did not survive at $60^{\circ}C$ for 60 minutes while ascospores at $80^{\circ}C$ for 120 minutes. Damages of fruit body of Agaricus bisporus caused by D. microsporus were maximum when the fruit bodies were infected at spawning and casing on the compost. The truffle disease could be controlled by basamid with $100{\sim}150 ppm$ treating on the compost after filling.

• The Korean Journal of Mycology
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• v.2 no.1
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• pp.7-14
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• 1974

These experiments were conducted to learn the antibiosis of microfloral organisms in the casing soil to Mycogone perniciosa, and interactions between M. perniciosa and Agaricus bisporus. The results obtained were as follows: 1. In vitro tests, the development of M. perniciosa was suppressed from the unidentified microfloral organisms in the casing soil. All the infected sporophores of A. bisporus occurred within an area applied with spore suspension of M. perniciosa as spot treatment and no infected ones in the area around the spot treated under cropping conditions. 2. In vitro tests, although the antagonistic relationship between M. perniciosa and A. bisporus was somewhat different in varying the kind of media, A. bisporus ultimately overgrew the colony of M. perniciosa. When spore inoculation of M. perniciosa was applied on the surface of grain spawn of A. bisporus and the mid layer of casing soil under cropping conditions, no infected sporophores were produced, whereas the infected sporophores were only produced on casing soil inoculated on the surface of casing soil with spore suspension.

• Journal of the Korean Wood Science and Technology
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• v.28 no.1
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• pp.55-64
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• 2000

Culture maturity assessment can be used to control fruiting body flush timing. Culture maturity of sawdust-based substrate was evaluated by using oak mushroom, (Lentinula edodes (Berk.) Pegler). The influence of substrate water potential (${\psi}$) on the growth and fruiting of three genotypes of L. edodes was also investigated. Glucosamine content revealed a peak at the fruiting body senescent stage. Glucosamine increased steadily to the sporophore senescent stage, and sharply declined at crop treatment. Lipid phosphate and ergosterol contents peaked at pinning and button break stages, respectively. Therefore lipid phosphate and ergosterol contents would be considered as the convenient measurement for judging culture maturity and fruiting potentials. The substrate pH values before inoculation and on the fruiting stage were varied from 6.3 to 4.0. This pH changes were detected as changes in color from bluish purple to yellow by direct bromphenol blue(BPB) spraying, and shown a good correlation with fruit body yield of the 1 st flush. Concerning water potential of the cultures, a slight reduction of water potential, -0.5MPa, stimulated mycelial and colony growths on liquid, agar and sawdust-based substrates. The water potential of well-colonized matured substrate was -0.7MPa and -4.0MPa, before and after the fruiting, respectively. Excellent water providing capacity (higher ${\psi}$) is expected to well-matured cultures with a high density of mycelial colonization. Also, the substrate water potential significantly affected by the interaction between genotypes and spawn run time.

• The Korean Journal of Mycology
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• v.40 no.1
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• pp.49-53
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• 2012

Hot-water extracted natural media were made from raw materials for mycelial culture of Pleurotus eryngii. Poplar sawdust, wheat bran and rice bran were used as substrates for hot water extraction. The mixed substrates of poplar sawdust, wheat bran, and rice bran with 50 : 20 : 30 (v/v/v, PWR523) and 50 : 30 : 20 (v/v/v, PWR532) were optimal for mycelial growth of P. eryngii, respectively. The hot-water extracted natural media from PWR523 and PWR532 showed a rapid mycelial growth and spawn running compared to PDA. There was no significant difference in mushroom yield when the mycelium grown on the hot-water extracted natural media was used as the inoculum source for producing fruit body.

• The Korean Journal of Mycology
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• v.46 no.3
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• pp.281-294
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• 2018

Molecular analysis using the internal transcribed spacer region sequences revealed that the strains used in this study, which were formerly identified as Panellus serotinus, are Panellus edullis. After Universal Fungal PCR Fingerprinting (UFPF) analysis, eight strains of P. edulis were divided into two groups. We conducted fundamental research on mycelial growth and sawdust cultivation to understand the cultural characteristics of eight wild P. edulis strains collected from Korean forests. All strains showed faster and denser mycelial growth on potato dextrose agar (PDA) than on other media (malt extract agar, Sabouraud dextrose agar). Optimal conditions for mycelial growth were: $20^{\circ}C$ on PDA, $25^{\circ}C$ on potato dextrose broth (PDB), and pH 5~8 on PDB at $25^{\circ}C$. Two strains (NIFoS 2407, 3993) were selected as excellent strains based on mycelial growth and density on PDA. NIFoS 2792 showed high cellulase activities on carboxymethyl cellulose (CMC) agar, and NIFoS 2387 and 2804 exhibited high laccase activities on ABTS-containing agar media. The mycelial growth of P. edulis was the fastest on Quercus acutissima and Q. mongolica sawdust media, and mycelial density was the highest on Quercus spp. sawdust-containing media. Sawdust cultivation of P. edulis was successful. The conditions were 80~85 days of cultivation period after spawn inoculation, 10~11 days for primordial formation at $17{\sim}18^{\circ}C$, and 15~20 days for fruiting growth. NIFoS 2804 and 3993 were selected as good strains in terms of cultivation period and mushroom production. These results could be useful for the artificial cultivation of P. edulis.