• KSBB Journal
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• 제23권2호
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• pp.158-163
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• 2008

버섯 균사체 혼합배양액을 이용하여 어류질병세균에 대한 항균 및, 항산화활성, 그리고 인체 암세포에 대한 항암 활성을 조사하였다. 항균활성 측정 결과 대부분의 시료에서 항균활성이 있는 것으로 나타났다. 그 중 YM broth에서 배양한 상황버섯과 운지버섯 균사체 배양 추출물이 가장 높은 활성을 띄었지만 10% 감귤추출물배지에서 배양한 상황버섯과 운지버섯 균사체 배양 추출물 또한 비교적 활성이 높아 합성배지가 고가임을 고려할 때 의의가 있다고 생각된다. 항산화 활성의 측정결과 10% 감귤추출물배지에서 배양한 상황버섯, 운지버섯, 꽃송이 버섯 균사체 배양추출물과 PD broth에서 배양한 상황버섯과 운지버섯 균사체 배양 추출물의 경우 1 mg/ml에서 90%에 가까운 라디컬 소거활성을 보였다. 항암활성 측정결과 10% 감귤추출액만을 첨가한 대조군보다 모든 실험구가 높은 활성을 나타냈으며 특히 10% 감귤추출물배지에서 배양한 상황버섯, 운지버섯 배양 추출액과 10% 감귤추출물배지에서 배양한 상황버섯, 운지버섯, 꽃송이버섯 균사체 배양 추출액이 높은 활성을 나타났다. 이러한 다양한 생리활성을 측정한 결과 최종적으로 감귤농축액이 첨가된 버섯균사체 배양 추출물들의 활성이 가장 좋은 것으로 나타났으며, 감귤의 천연배지로서의 이용가능성을 확인할 수 있었다. 따라서 감귤농축액 및 버섯균사체 혼합 배양을 이용한 다양한 사료첨가제 및 신소재의 개발이 가능할 것이다.

• 생명과학회지
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• 제17권10호
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• pp.1434-1440
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• 2007

본 연구는 넙치의 면역반응에서 버섯균사체 (상황버섯, 운지버섯) 혼합배양추출물의 효과에 대해서 연구하였다. 혼합배양추출물은 넙치에 대해서 성장, 혈액, 라이소자임활성, 백혈구, 식세포활성 및 Vibrio anguillarum에 대한 질병저항성에 관해서 평가되었다. 성장에 있어서 버섯균사체 혼합 배양 추출물을 섭이한 그룹의 체중은 52 g 전장은 2.1 cm가 대조구보다도 증가하였다. 혈액학적으로 버섯균사체 혼합 배양추출물을 경구투여 했을 때 글루코오스의 양이 증가하였다. 그러나 각 그룹에 대한 COT (glutamic oxaloacetic transaminase), GPT (glutamlc pyruvic transamlnase), TG, TP, LDH (lactate dehydronase)의 유의적인 차이는 없었다. 라이소자임 활성은 대조구 보다 실험구에서 80%의 높은 결과를 얻을 수 있었으며, 식세포 활성은 실험구에서 대조구 보다 60% 높은 활성을 보였다. 라이소자임 활성과 식세포 활성의 결과 경우에는 12주후에 다소 감소하는 양상을 보였지만, 대조구 보다는 높은 활성을 보였다. 그리고 $7{\times}10^8\;CFU$ 농도로 어류 병원성 세균인 Vibrio anguillarum를 어류 당 투여하여 병원성 테스트를 한 결과 누적 폐사율을 대조구와 비교해 보았을 때 25%의 높은 생존율을 보였다.

• 대한한의학회지
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• 제29권1호
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• pp.85-94
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• 2008

Objectives : The purpose of this study was to investigate effects of extract from mixed culture with Tricholoma matsutake mycelium and Cordyceps militaris mycelium in oriental medicine and cereal midium (OCM) considered effective for the treatment of diabetes. Methods : We examined antioxidant activity and $\alpha$-glucosidase inhibit activity of OCM and orally administered OCM extracts to Streptozotocin-induced diabetic ratsat the dose of 100mg/kg, 200mg/kg and 300mg/kg body weight. Results : Total polyphenol content of extracts of OCM was 26.41 mg/g, DPPH radical scavenging activity was 32.24% and SOD-like activity was 31.43%. $\alpha$-glucosidase inhibit activity showed 85.3% of acarbose. Blood glucose levels of the OCM extracts-treated groups were lower than those of the diabetic control group (DC), total cholesterol and triglyceride contents were similar to the acarbose-treated group (PC) and ALT, AST were lower than the DC and PC groups. The 300 mg/kg body weight administered group (UM-300) were superior to other OCM-treated groups (UM-100, UM-200). Conclusions : These results showed extract from mixed culture with Tricholoma matsutake mycelium and Cordyceps militaris mycelium in oriental medicine and cereal midium has potentiality to beused in pharmaceutical products and medicinal food for preventive and treatment diabetes mellitus.

• Mycobiology
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• 제51권2호
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• pp.94-108
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• 2023

Termitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO₂ conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a coadjuvant therapy in malignant diseases.

• 대한본초학회지
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• 제23권1호
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• pp.1-8
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• 2008

Objectives : The purpose of this study was to investigate extracts from mixed culture of Oriental medicines and cereal medium and mycelia of Trichloma matsutake and Cordyceps militaris by fermentation to develop new material for pharmaceutical products and medicinal food, Methods : To evaluate physiological activities of OCM extracts, we examined antioxidant activity(total polyphenol contents, electronic donating ability, SOD-like activity), ${\beta}$-glucan contents, nitric oxide production and cytotoxicity by MTT assay. Results : Total polyphenol contents of fermented OCM(UF) and non-fermented OCM(UM) extracts were more than 40% UM and UF of DPPH radical scavenging activity was 25.67%, 23.43% respectively. Total polyphenol content of non-fermented extract (UME) was 12.57%, while that of fermented extract(UFE) was 7.05%. SOD like activity showed UM 85.35%, UF 76.18%, UME 58.42%, UFE 72.21%. UME, and UFE 31.43%, ${\beta}$-glucan contents of UME and UFE were more than 40%. NO productions of UME, and UFE showed a LPS dose dependent tendency. Cytotoxicity on Raw 264.7 cell showed more than 90% viability. Inhibitory effect of UFE on HT1080 cell growth was higher than UME. Conclusions : These results showed that extracts from mixed culture of mushroom mycelium and OCM have physiological activities which can be used in pharmaceutical products and medicinal food.