• 제목/요약/키워드: Muscle Length

검색결과 671건 처리시간 0.033초

Regulated Expression of Nebulin by Transfection of Green Fluorescent Protein-Tagged Nebulin Fragments in Cultured Chicken Myoblast

  • Park, Su-Jung;Kim, Ji-Hee;Ko, Han-Suk;Kim, Chong-Rak;Kim, Han-Do;Kang, Ho-Sung
    • Biomedical Science Letters
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    • 제7권4호
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    • pp.167-172
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    • 2001
  • Nebulin is an approximately 700 kDa filamentous protein in vertebrate skeletal muscle. It binds to the Z line and also binds side-by-side to the entire thin actin filament in a sarcomere. The correlation of nebulin size with thin filament length have led to the suggestion that nebulin acts as a molecular ruler for the length of thin filaments. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain. SH3 domains have been identified in an ever-increasing number of proteins important for a wide range of cellular processes, from signal transduction to cytoskeleton assembly and membrane localization. However, the exact physiological role of SH3 domains remains, in many cases, unclear. To explore the role of nebulin SH3 in the cytoskeletal rearrangement that accompanies myoblast differentiation, we transfected sense and antisense nebulin SH3 domain fused to enhanced green fluorescent protein in myoblast. Cells expressing nebulin SH3 fragment showed decrease of cell-cell adhesion, and cells transfected with antisense nebulin SH3 gene showed a rounded cell morphology and loss of cell-matrix adhesion. No alteration in cell shape and differentiation were observed in control cells expressing enhanced green fluorescent protein. Perturbation of nebulin altered the cell shape and disrupted cell adhesion in myoblast, demonstrating that nebulin can affect cytoskeleton rearrangement.

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The Effect of Therapeutic Ultrasound and Static Stretching of the Hamstring Muscle on Flexibility and Static Balance Ability (초음파 치료와 정적 스트레칭이 뒤넙다리근의 유연성과 정적균형에 미치는 영향)

  • Cho, Seung-bong;Park, Mi-young;Won, Ji-sun;Kim, Ye-lin;Hong, Joon-taek;Kim, Seong-ho
    • Journal of Korean Physical Therapy Science
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    • 제26권1호
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    • pp.45-53
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    • 2019
  • Background: The purpose of present study was to examine the effect of ultrasound therapy and static stretching on hamstring length and balance. Design: Randomized Controlled Trial. Methods: A total of 30 adults in S college, Seongnam city, Gyeonggi-do, between the ages of 20-30 were randomly assigned to two groups. Group 1 (n=15) had ultrasound therapy, called US group. Group 2 (n=15) had static stretching, called SS group. Measurements were taken prior to starting the program and after completing the experiment using Finger to floor test and Active knee extension test to get the results of hamstring flexibility and the ability of static balance. Paired t-test was used to compare each group pre-test values to post-test values and to compare US post-test values to SS post-test values on PASW 18.0. Results: 1) Both hamstring SS group and US group had significant increase (p<.05) in Finger to floor test and Active knee extension test. 2) Dominant leg standing had significant change (p<.05) only in US group. 3) There was no significant difference between US group and SS group. Conclusion: Results showed that US group and SS group had an effect on changes in hamstring length (p<.05). However, they did not show a significant increase in static balance. Further effective studies on hamstring SS group and US group were needed based on this examination.

The Effects of Instrument Pilates Exercise with EMS on Pain, Lymphedema and Range of Motion of Upper Extremity in Subjects after Mastectomy : Randomized Cross-over Design (국소적 전기근육자극을 결합한 기구 필라테스가 유방절제술 후 유방암 환자의 통증, 림프부종 및 팔 관절가동범위에 미치는 효과 : 무작위 교차실험 연구)

  • Kang, Chae-Young;Park, Hyun-Ju;Chon, Seung-Chul
    • Journal of The Korean Society of Integrative Medicine
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    • 제10권4호
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    • pp.113-120
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    • 2022
  • Purpose : Lymphedema is a common complication in mastectomy patients and is usually characterized by pain, swelling, and limited range of motion (ROM) in the arm. Electromyostimulation (EMS) is widely used for the rehabilitation and recovery of subjects with various neuromusculoskeletal disorders after breast cancer. However, EMS has not yet been used in many Pilates exercises. This study was aimed at comparing the effects of instrument Pilates integrated with EMS on pain, lymphedema, and ROM of the upper extremity (UE) in breast cancer subjects after mastectomy. Methods : Nine female breast cancer subjects who had undergone mastectomy participated in the study. The subjects underwent instrument Pilates with EMS (experimental group) or instrument Pilates only (control group). Pain, lymphedema, and ROM of the UE were measured using the visual analog scale (VAS), the circumference length of the UE, and the ROM of the UE. The Wilcoxon signed-rank test was used to compare the pain, lymphedema, and ROM of the arm before and after the intervention, and the Mann-Whitney U test was used to compare the two groups. The statistical significance level was set to p < .05. Results : In the experimental group, there were significant differences in pain (p<.05) and UE circumference (p<.05) before and after intervention. However, there was no significant difference between the two groups in VAS (p>.05) or circumference length of the UE (p>.05). There was one significant difference between the groups in terms of internal rotation of the ROM of the UE (p<.05). Conclusion : These results show that instrument Pilates exercises combined with EMS may positively affect the internal rotation of the ROM of the UE in breast cancer patients after mastectomy, thus contributing to existing knowledge about instrument Pilates using EMS for the effective management of in breast cancer subjects after mastectomy.

Relationship to the superficial radial nerve and anatomic variations of the first extensor compartment in Thai population: a basis for successful de Quervain tenosynovitis treatment

  • Krittameth Pasiphol;Sithiporn Agthong;Napatpong Thamrongskulsiri;Sirikorn Dokthien;Thanasil Huanmanop;Tanat Tabtieng;Vilai Chentanez
    • Anatomy and Cell Biology
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    • 제57권2호
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    • pp.246-255
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    • 2024
  • Knowledge of the superficial radial nerve (SRN) relationship and anatomic variations of the first extensor compartment (1st EC) will contribute to a better outcome of de Quervain tenosynovitis treatment. We dissected 87 embalmed cadaveric wrists to determine the relationship of the SRN, the 1st EC length, distance from the proximal and distal 1st EC borders to radial styloid process (RSP), abductor pollicis longus (APL) and extensor pollicis brevis (EPB) tendon slip numbers, and the presence of septum. Our results revealed SRN crossing over the 1st EC in 59.5%. The lateral branch of the superficial radial nerve to the 1st EC midline in most cases (61.9%) except for one specimen, where lateral antebrachial cutaneous nerve was the closest. Distances from proximal and distal 1st EC borders to the RSP were 19.7±4.1 mm and 7.6±1.8 mm, respectively. Extensor retinaculum (ER) width over 1st EC (1st EC length) was 14.8±3.2 mm. Complete and incomplete septa were found in 17.2%, and 42.5%, respectively. The most frequent APL tendon slip number in the compartment was two in overall 47 specimens (54.0%). Almost all compartments (85 specimens; 97.7%) contained one EPB tendon slip. We detected bilateral EPB absence in one cadaver. Moreover, we recorded a tendon slip from extensor pollicis longus traveling into 1st EC bilaterally in one cadaver and observed the EPB muscle belly extension into 1st EC in 9 wrists. Awareness of 1st EC anatomic variations would be essential for successful surgical and nonsurgical outcomes.

A Study on the Growth of Pen Shell, Atrina pectinata japonica Transplanted into Duekryang Bay in Southern Korea I. Environmental Factors and Transplanted Effect on Different Shell Size Groups (득량만에 이식한 키조개, Atrina pectinata japonica의 성장에 관한 연구 I. 양식장 환경 및 각장 크기별 이식효과)

  • 양문호;최상덕;노용길;김성연;정춘구
    • Journal of Aquaculture
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    • 제11권2호
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    • pp.193-201
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    • 1998
  • This study was carried out to investigate the enviromental quality and the growth of transplanted pen shell, Atrinna pectinata japonica. Followings are the results of growth of transplanted pen shell with respect to the shell size groups from the natural habitat (Usando) in May 1995, and cultivated upto November in the transplantated area (Soomoonri). The water depth of transplantated area andnatural habitat were 3m, 20~25m, respectively. The seawater temperature of the two culturing farms were ranged of 10.9~$27.8^{\circ}C.$, 8.5~$30.0^{\circ}C.$, respectively at the lowest in November adn the highest in July. The seawater salinity of the two areas were ranged of 29.54~35.26$^0\prime\infty$, 28.75~36.31$^0\prime\infty$, respectively at the lowest in July and the highest in November. The phosphoric acid ($PO_4$-P) of the two areas were 0.09~$1.14 ^{\mu}$g-at/l, 0.23~$1.33 ^{\mu}$g-at/l, respectively at the lowest in June and the highest in September. The bottom type of the two areas was a silty mud, 85.23% (82.17~87.26%) in natural habitat and 92.12% (90.76~92.94$^0\prime\infty$) in transplanted area. In this study area, phytoplankton were composed of 19 species. Of these 19 species, Skeletonema costatum was dominant species in seawater between natural habitat and transplantatied area, and 157 cells/ml, 165 cells/ml at August respectively. Stock of phytoplankton in transplantated area were more than those of natural habitat except June and November. The growth of shell length, shell height, total weight, soft part weight and posterior adductor muscle weight of pen shell on different size groups (SL 10, 10~15, 15~20, 20cm) were excellent in shell length of 10cm group, and 99.32%, 107.66%, 871.09%, 951.26% and 1,223.76%, respectively. The survival rate of pen shell was 98.10% in the shell length of 10cm groups, 90.35~94.76% in the others groups. The growth of shell length, total weight, soft part weight and posterior adductor muscle weight of pen shell in transplantated area were more 1.3, 2.6, 2.7 and 4.5 times than those of natural habitat.

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Nucleotide and Deduced Amino Acid Sequences of Rat Myosin Binding Protein H (MyBP-H)

  • Jung, Jae-Hoon;Oh, Ji-Hyun;Lee, Kyung-Lim
    • Archives of Pharmacal Research
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    • 제21권6호
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    • pp.712-717
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    • 1998
  • The complete nucleotide sequence of the cDNA clone encoding rat skeletal muscle myosin- binding protein H (MyBP-H) was determined and amino acid sequence was deduced from the nucleotide sequence (GenBank accession number AF077338). The full-length cDNA of 1782 base pairs(bp) contains a single open reading frame of 1454 bp encoding a rat MyBP-H protein of the predicted molecular mass 52.7kDa and includes the common consensus 1CA__TG' protein binding motif. The cDNA sequence of rat MyBP-H show 92%, 84% and 41% homology with those of mouse, human and chicken, respectively. The protein contains tandem internal motifs array (-FN III-Ig C2-FN III- Ig C2-) in the C-terminal region which resembles to the immunoglobulin superfamily C2 and fibronectin type III motifs. The amino acid sequence of the C-terminal Ig C2 was highly conserved among MyBPs family and other thick filament binding proteins, suggesting that the C-terminal Ig C2 might play an important role in its function. All proteins belonging to MyBP-H member contains `RKPS` sequence which is assumed to be cAMP- and cGMP-dependent protein kinase A phosphorylation site. Computer analysis of the primary sequence of rat MyBP-H predicted 11 protein kinase C (PKC)phosphorylation site, 7 casein kinase II (CK2) phosphorylation site and 4N-myristoylation site.

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The Effects of Falls Prevention Exercise on Functional Fitness in Elderly (낙상예방체조가 노인의 체력 변화에 미치는 영향)

  • Park, Sung-Kyu;Kim, Ji-Sung;Sin, Hyung-Su
    • Journal of Korean Physical Therapy Science
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    • 제15권4호
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    • pp.1-9
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    • 2008
  • Background: This study was to investigate effects of falls prevention exercise on functional fitness in elderly. Methods: A total of 45 healthy over 65years old adults (men=15, women=30) who were participated in this study. Exercise were involved in the period of 12 week complex physical training for around 50 minutes a day, three times per week. We measured subjects' physical activity levels by short physical performance battery(SPPB) test, timed up & go(TUG) test, one leg standing test(OLST) with closed eyes and maximal step length (MSL) test. Results: After falls prevention exercise, there were statically significant differences in SPPB, TUG, OLST and MSL test (P<.01) between pre and post test. There were statically significant differences in SPPB, TUG, OLST, MSL between pre and post test in men and women. There were statically significant differences between men and women in SPPB, TUG, OLST and MSL test. Conclusion: Falls prevention exercise had significant effects on physical fitness level in elderly, with a result increase muscle strength, balance ability and it might prevent falls.

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The influence of hysterectomy on LBP investigated through Oriental Medicine theory : Clinical study. (자궁적출술이 요통에 미치는 영향에 관한 한의학적 연구)

  • Kang, In;Song, Joo-Hyun;Moon, Ja-Young;Lim, Myung-Jang;Cho, Jae-Hee;Lee, Hyo-Eun
    • The Journal of Churna Manual Medicine for Spine and Nerves
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    • 제2권2호
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    • pp.117-122
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    • 2007
  • Objectives : The purpose of this study is to investigate the influence of hysterectomy on Low Back Pain(LBP) and to describe it through Oriental Medicine theory. Methods : Research was conducted by history taking of 1849 female LBP patients who visited Jaseng Oriental Medicine Hospital. Investigation was focused on the number of patients who underwent hysterectomy, and the length of time developing LBP after the surgery. Results : 178 patients(9.6%) underwent hysterectomy among 1849 patients, and it was higher than general rate of hysterectomy. In Oriental medicine, this result can be explained that it is caused by the lack of water function of spleen(腎水) from the absence of uterus, consequently it makes muscle weak and does harm to Blood and Meridian. Conclusions : History of hysterectomy has a significant influence on the development of LBP.

Analysis of Trunk Muscle Fatigue as the Frequency of Lifting/Lowering and Recovery Time Change (들기/내리기 빈도와 회복시간 변화에 따른 몸통 근육의 피로도 분석)

  • Lee, Tae-Yong;Kim, Jeong-Ryong;Sin, Hyeon-Ju
    • Journal of the Ergonomics Society of Korea
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    • 제25권2호
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    • pp.197-204
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    • 2006
  • The purpose of this study was to investigate the relationship between the cumulative fatigue of trunk muscles and the period of recovery time during repetitive lifting and lowering task with two different frequencies(4 times/min and 6 times/ min). Eight healthy males with no prior history of LBD(low back disorders) volunteered for this study. Subjects had 2, 3, 4, and 5 minutes recovery time respectively while they were preforming the lifting and lowering task at 15% level of MVC. EMG signals from six trunk muscles were collected and the fatigue level was analyzed quantitatively. In results, the fatigue levels of LES(left elector spinae), RLD(right lattissimus dorsi), LLD(left lattissimus dorsi), RRA(right rectus abdominis) and LRA(left rectus abdominis) were recovered when 3 minutes recovery time was given at 4 times/min frequency. However, the fatgue level of RES(right elector spinae) was recovered when 4 minutes recovery was given. On the other hand, when 6 times/min frequency was used, the RLD, LLD, RRA and LRA were recovered at 5 minutes of recovery time. But for RES and LES, it took longer than 5 minutes to be recovered. This results can be applied to design the adequate length of recovery time to control the cumulative fatigue of trunk muscles in industry with repetitive lifting and lowering task.

Sex Difference in the Range of Pelvic Tilt in Sitting and Standing Among Korean Young Adults

  • Yoon, Jangwhon
    • Physical Therapy Korea
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    • 제27권2호
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    • pp.149-154
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    • 2020
  • Background: The range of pelvic tilt is one of modifiable risk factors in preventing the lower back pain. Objects: The purpose of this study were to compare the range of pelvic tilt motion by testing position and sex. Methods: One hundred five young adults (61 females and 44 males) agreed to participate in measuring the anterior and posterior pelvic tilt with the PALM (Palpation Meter) in sitting and standing. The range of pelvic tilt motion was defined as the difference between the pelvic anterior and posterior tilt angles. Results: In general, the anterior pelvic tilt was greater (p < 0.01) in standing than in sitting and the posterior pelvic tilt was lesser (p < 0.01) in sitting than in standing. The anterior pelvic tilt in sitting and standing was greater (p < 0.01) in the females than in the males. However, the effect of sex on the posterior pelvic tilt was only significant in sitting (p < 0.01), but not in standing (p = 0.78). The range of pelvic tilt was greater (p = 0.03) in sitting but not significantly (p = 0.07) affected by the sex. Conclusion: The pelvic tilt motion in these young adults showed large variability and further studies are needed to understand better its relationship to the prevalence of the lower back disorders.