• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.66-71
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• 2002

We investigated the effect of fish oil (FOS) on growth performance, ruminal metabolism and fatty acid composition and physical characteristics of longissimus muscle in 10 steers and 10 bulls of Korean cattle. Concentrates diet was supplemented with FOS at 5% of the diet. FOS contained 3.34% eicosapentaenoic acid (EPA) and 24.87% docosahexaenoic acid (DHA) of total fatty acids by weight. Average daily weight gain and feed efficiency were not affected (p>0.871) by FOS, but feed intake was decreased. FOS had lower (p<0.003) pH and higher (p<0.001) $NH_3$-N than that of control. There was a treatment effect (p<0.001) for serum cholesterol concentrations. FOS increased (p<0.009) concentrations of n-3 fatty acids, including linolenic, EPA and DHA in longissimus muscle. Physical traits were significantly (p<0.015) changed by feeding FOS except for pH and lightness (L). We concluded that the fatty acid composition and physical properties of the muscle in fattening Korean cattle can be altered by feeding 5% FOS.

• Physical Therapy Korea
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• v.20 no.1
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• pp.55-63
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• 2013

This study investigated the effects of axillary crutch length on trunk muscle activity and lumbo-pelvic-hip complex movements during crutch gait. Eleven healthy men participated in this study. The participants performed a three-point gait with optimal, shorter, and longer crutch lengths. Weight-bearing (WB) side was determined as the dominant leg side. The electromyography (EMG) activity of the bilateral rectus abdominis (RA) and erector spinae (ES) muscles and lumbo-pelvic-hip complex movements were monitored using a three-dimensional motion system with wireless surface EMG. Differences in the EMG activity of RA and ES muscles and range of motion (ROM) of lumbar spine, pelvis, and hip among conditions were analyzed using one-way repeated-measures analysis of variance, and a Bonferroni correction was conducted. There was less RA muscle activity on the WB side under the optimal crutch length condition compared with shorter and longer crutch length conditions (p<.05). The EMG activity of the RA muscle on the non-WB side and ES muscle on the WB side were significantly decreased under the optimal crutch length condition compared with shorter crutch length condition (p<.05). No significant differences in the EMG activity of the ES muscle on the non-WB side and ROM of lumbo-pelvic-hip complex were found among conditions (p>.05). These findings indicate that the optimal crutch length improves the trunk muscle efficiency during crutch gait.

• Journal of Nutrition and Health
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• v.25 no.6
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• pp.485-491
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• 1992

The effects of varying doses(1, 4 and 10mg/kg body weight/day) of testosterone propionate (TP) on body weight gain and composition and energy and muscle protein metabolism were investigated in female rats. TP had no effect on food intake at any dose but injection of 1mg/kg resulted in an in crease in body weight gain which was associated with increases in body protein and fat. At higher doses(4 and 10mg/kg) body protein content was still increased but body fat was not affected. Increases in energy gain and gross energetic efficiency were observed at a dose of 1mg/kg but neither parameter was affected at other doses. The mass protein and RNA content of gastrocnemius muscle were incerased by TPbut the ratio of RNA to protein and the rate of muscle protein synthesis measured in vivo were not affected at any dose of TP The results indicate that the effect of testosterne on body composition are highly dose-dependent and the anabolic action of testosterone is not through stimulation of protein synthesis.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.1029-1036
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• 2017

Objective: In the livestock industry, the regulatory mechanisms of muscle proliferation and differentiation can be applied to improve traits such as growth and meat production. We investigated the regulatory pathway of MyoD and its role in muscle differentiation in quail myoblast cells. Methods: The MyoD gene was mutated by the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology and single cell-derived MyoD mutant sublines were identified to investigate the global regulatory mechanism responsible for muscle differentiation. Results: The mutation efficiency was 73.3% in the mixed population, and from this population we were able to establish two QM7 MyoD knockout subline (MyoD KO QM7#4) through single cell pick-up and expansion. In the undifferentiated condition, paired box 7 expression in MyoD KO QM7#4 cells was not significantly different from regular QM7 (rQM7) cells. During differentiation, however, myotube formation was dramatically repressed in MyoD KO QM7#4 cells. Moreover, myogenic differentiation-specific transcripts and proteins were not expressed in MyoD KO QM7#4 cells even after an extended differentiation period. These results indicate that MyoD is critical for muscle differentiation. Furthermore, we analyzed the global regulatory interactions by RNA sequencing during muscle differentiation. Conclusion: With CRISPR/Cas9-mediated genomic editing, single cell-derived sublines with a specific knockout gene can be adapted to various aspects of basic research as well as in functional genomics studies.

• The Journal of Korean Academy of Prosthodontics
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• v.36 no.1
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• pp.81-103
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• 1998

The purpose of this study was to compare and evaluate the differences in masticatory performance, muscle activity, and patterns of occlusal contact between persons with natural dentition and removable partial denture wearers. Twenty healthy adult subjects with more than 28 teeth and thirteen removable partial denture wearers that classified Kennedy classification I was selected. The degree of pulverized rice and peanut was measured and analyzed by means of sieving method to compare the masticatory performance. For the muscle activity, EMG was recorded in selected muscles(Temporalis and masseter muscle) during mastication and resting state. The occlusal record in maximal intercuspation was taken with a silicone occlusal bite registration material for analysis of the patterns of occlusal contact. The obtained results were as follows: 1. When chewed peanuts, masticatory performance ratio at 10-sieve size was 81.31% in natural dentition group. In removable partial denture wearer, 27.01% without RPD and 69.09% with RPD. When chewed rice, 42.04%, 11.87%, and 21.58%, respectively. The differences of masticatory performance ratio between groups were statistically significant at the 0.05 level. 2. The mean EMG value in resting state was $1.06{\mu}V$ on temporal muscle, $0.98{\mu}V$ on masseter muscle in natural dentition group. In removable partial denture wearers, $1.13{\mu}V$ on temporal muscle, $1.05{\mu}V$ on masseter muscle without RPD and $1.11{\mu}V$ on temporal muscle, $1.04{\mu}V$ on masseter muscle with RPD. 3. The mean EMG value during mastication was $45.64{\mu}V$ in natural dentition group, and in removable partial denture wearers, $22.06{\mu}V$ without RPD and $31.01{\mu}V$ with RPD when chewed peanuts. When chewed rice, $45.24{\mu}V,\;25.53{\mu}V\;and\;32.14{\mu}V$, respectively. The differences of mean masticatory EMG value between groups were statistically significant at the 0.05 level. 4. The number of posterior occlusal contact point was 20.15 in natural dentition group and 11.92 in removable partial denture wearers. The area of perforated surface was $16.50mm^2$ in natural dentition group and $6.06mm^2$ in removable partial denture wearers. The area of contact surface was $78.93mm^2,\;51.52mm^2$, respectively. 5. The area of contact surface was effective to masticatory performance ratio in natural dentition group and removable partial denture wearers (p<0.05). From these results, it is concluded that in partially edentulous patient, masticatory efficiency can be improved by removable partial denture wearing, and for efficient mastication, tight occlusal contact surface shoud be maintained by maximum support that is provided from mucosa.

• Journal of the Korean Society of Clothing and Textiles
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• v.44 no.4
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• pp.739-748
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• 2020

In this study, a wrist protector was manufactured and designed using 3D printing technology to prevent wrist pain when riding a bicycle with a high forward-tilted saddle. The efficiency of the design was analyzed in objective and subjective evaluations. First, seven women in their twenties selected their most preferred design by subjective evaluation from among three 3D wrist protectors. Then electromyography (EMG) was measured and subjective sensations were evaluated while wearing and not wearing the preferred 3D wrist protector as selected by cyclists. Experimental results showed that a wrist-bending angle of 30 degrees was most favored. When the 3D wrist protector was worn, muscle activity was smaller at the flexor carpus radialis position than when the 3D wrist protector was not worn; muscle fatigue was also lower. In the subjective evaluation, the 3D wrist protector was more comfortable than no protector, and wrist fatigue was also lower. In addition, the wrist protector was rated positively for its design suitability, size adequacy, ease of operation, and fit; however, cyclists felt that a little more cushioning was needed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.651-659
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• 2023

The effect of bio-logger tagging location on blood characteristics and bio-logger attachment efficiency in spotted sea bass (mean body weight 2356.7 g) was investigated. The fish were tagged at four different tagging locations: no-tag (control), operculum attachment (OA), dorsal muscle attachment (DA), and cauda peduncle muscle attachment (CA). The blood properties and bio-logger attachment efficiencies were examined on days 1, 7, 14, and 35 after tagging the bio-logger at each tagging location. During the experimental periods, the concentrations of hematocrit and hemoglobin in whole blood, and GOT (glutamic oxaloacetic transaminase), GPT (glutamic pyruvic transaminase), total protein (TP), glucose, total cholesterol, cortisol, and superoxide dismutase in plasma were not affected by the attachment location of the bio-logger, however, the TP concentration was significantly lower in OA than in the control group on day 7. After tagging for 35 days, the efficiencies of bio-logger attachment in the OA, DA, and CA after tagging for 35 days were 33.3%, 100.0%, and 33.3%, respectively. These results indicate that, in our experimental condition, the most appropriate bio-logger attachment location is DA, providing basic information on bio-logger utilization methods for ecological and biological biotelemetry surveys of the spotted sea bass.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.4
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• pp.488-494
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• 2014

Twenty-eight Brangus cattle were used to determine the effect of copper and selenium supplementation on performance, feed efficiency, composition of fatty acids in Longissimus dorsi (LD) muscle, and cholesterol concentration in serum and in LD muscle and enzymes activities, reduced glutathione (GSH) and oxidized glutathione (GSSG). The treatments were: i) Control, without copper (Cu) and selenium (Se) supplementation; ii) Se, 2 mg Se/kg of dry matter such as sodium selenite; iii) Cu, 40 mg Cu/kg of dry matter such as copper sulfate; iv) Se/Cu, 2 mg Se/kg of dry matter such as sodium selenite and 40 mg Cu/kg of dry matter such as copper sulfate. LD muscle fatty acid composition was not influenced by the treatments (p>0.05). The serum concentration of cholesterol was not influenced by the treatments (p>0.05), however, the concentration of cholesterol in LD was lower in cattle supplemented with copper and selenium (p<0.05). Oxidized glutathione and reduced glutathione increased (p<0.05) with Cu, Se and Se/Cu supplementation. The supplementation of copper (40 mg/kg DM) and selenium (2 mg/kg DM) altered the metabolism of lipids in confined Brangus cattle, through a decrease in cholesterol deposition in the LD, possibly by changing the ratio between reduced glutathione/oxidized glutathione. Copper and selenium supplementation improved animal performance and feed efficiency (p<0.05) when compared to the control group, providing advantages in the production system, while also benefiting consumers by reducing cholesterol concentration in the meat.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.361-383
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• 1987

This thesis was designed to find out whether the cadmium content of hair in living animals reflects the status of cadmium accumulation in internal organs or muscles so that this status can be used as a diagnostic method. Forty-five rats were divided into 4 experimental groups and one control group. The control group received a basal diet which contained zinc $100{\mu}g/g$, iron $80{\mu}g/g$ and copper $135{\mu}g/g$. The experimental group received experimental diets sllpplemented with 1, 10, 100 or $1,000{\mu}g/g$ cadmium as cadmium sulfate. Rats were dissected on the 28th or 56th day of experimental periods and tissues, blood and hair samples were taken, dried and burned to ash and analysed. The cadmium, iron zinc and copper concentrations were measured with an atomic absorption spectrophotometer. In addition feed efficiency and hematological changes were observed. The results obtained can be summerized as follows: A marked decrease in feed intake, weight gain and feed efficiency were observed from 1 or 2 weeks of experimental periods, which was in accordance with the dosage and periods. The cadmium contents of kidney, liver, spleen and muscle were significantly increased in all experimental rats; the accumulation was marked in kidney and liver tissues. It was shown that the cadmium contents of hair reflects the cadmium accumulation in internal organs and muscle; the higher the cadmium levels of diet and of rat tissues, the higher the cadmium content of hair. In the $100{\mu}g/g$ group of rats erythrocyte counts and. hemoglobin concentrations were decreased. A significant(p<0.01, p<0.05) increase in iron contents of kidney, liver and muscle was observed in 10, 100, $1,000{\mu}g/g$ groups of rats on the 28th day of experiment. A significant (p<0.01, p<0.05) increase in zinc contents of kidney, liver and muscle was observed in all experimental rats. On the other hand, serum zinc concentration was decreased. A significant(p<0.01) increase in copper contents in the liver was observed on the 28th day in $100{\mu}g/g$ and $1,000{\mu}g/g$ groups of rats. From these results it may be concluded that the analysis of hair for cadmium is a useful diagnostic approach for the cadmium status of internal organs and muscle in living animals.

• Journal of Nutrition and Health
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• v.35 no.8
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• pp.825-833
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• 2002

The effects of dietary supplementation of Eleutherococcus senticosus, taurine and carnitine on maximal endurance exercise performance along with other related parameters were evaluated in rats that underwent aerobic exercise training for 6 weeks. Thirty-two male rats (4 weeks old) were randomly divided into 4 groups, and fed experimental diets and/or aerobic exercise trained according to the protocol: SC (sedentary control group), EC (exercise-trained control group), EE (exercise-trained Eleutherococcus senticosus-supplemented group), and EETC (exercise-trained Eleutherococcus senticosus, taurine and carnitine-supplemented group). The food efficiency ratio of EC rats was significantly lower than the value for SC rats (p < 0.01). Exercise-trained control animals (92 $\pm$ 8.8 min) could run significantly longer until exhausted on the treadmill than sedentary control rats (11 $\pm$ 0.8 min) (p < 0.001). Animals fed an Eleutherococcus senticosus-supplemented diet, and an Eleuthherococcus sonticosus, taurine and carnitine- supplemented diet while undergoing aerobic exercise training for 6 weeks exhibited, respectively, 8 and 5 minutes longer running performance until exhausted than the rats fed the control diet. The gastrocnemius muscle glycogen concentration of the rats, measured at 48 hours post maximal exercise performance test, was 43% higher in EC rats than the value for SC rats (p < 0.05), but was not different among EC, EE, and EETC rats. The mitochondrial citrate synthase activity of the soleus muscle was significantly higher in EC rats compared to the value for SC rats (p < 0.01), and showed a tendency to increase, without statistical significance, in EE or EETC rats compared to the value for EC rats. These results indicate that aerobic exercise training for 6 weeks significantly improved maximal exercise performance, muscle glycogen content along with citrate synthase activity, which are important in the energy metabolism of muscle under aerobic exercise. Dietary supplementation of Eleutherococcus senticosus in rats while undergoing aerobic exercise training improved maximal endurance exercise performance without significantly affecting muscle glycogen content and enzyme activities involved in energy metabolism during exercise. Taurine and carnitine supplementation failed to show an additive effect on maximal endurance exercise performance when consumed along with Eleutherococcus senticosus.