• PNF and Movement
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• 제21권1호
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• pp.107-117
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• 2023

Purpose: The aim of this study is to compare the immediate effects of weight-assisting methods on vastus medialis oblique (VMO) and vastus lateralis (VL) muscle activation, on the VMO/VL muscle activation ratio, and on muscle onset time in healthy subjects when ascending stairs. Methods: Healthy participants were randomly assigned to the belt group (n = 11), hand group (n = 11), and control group (n = 11). In the belt group, a belt was wrapped around the sacrum and pulled forward with both hands, moving the center of weight forward, while ascending stairs. The hand group grasped the hips with both hands and climbed stairs, assisting their weight from the rear and moving the center of weight backward, and the control group climbed the stairs without any intervention. Results: Muscle activation of the VMO decreased significantly after the intervention in the belt and hand groups, and activation of the VL muscle in both groups showed a greater decrease than that of the VMO muscle. Further, the VMO/VL muscle activation ratio increased significantly, with an improvement shown in the order of the belt group, hand group, and control group, while muscle onset time also improved in the order of the belt group, hand group, and control group. Conclusion: The belt group demonstrated the greatest effect across all dependent variables, confirming that in clinical practice, these two weight-assisting methods are more effective interventions during stair ascent for patients with knee joint instability, pain, and imbalance than no assistance.

• 한국보육지원학회지
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• 제8권5호
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• pp.5-24
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• 2012

본 연구는 자연 속에서 자연물을 가지고 손끝놀이를 활용한 자연친화적 손끝놀이프로그램이 유아의 소근육 운동기능 발달과 창의적 사고력에 미치는 효과는 어떠한가를 알아보는데 그 목적이 있다. 연구의 목적을 달성하기 위해 B시에 위치한 H어린이집 만5세 유아 14명과 P어린이집 만5세 유아 14명을 대상으로 유아의 소근육 운동기능 발달을 측정하기 위해 강병동(2002)이 연구한 '소근육 운동기능 발달 평가도구'와 창의적 사고력 발달을 측정하기 위한 토랜스의 창의력 검사 중 도형검사를 김영채(2004)가 표준화시킨 한국판 창의성 도형검사를 사용하였다. 그에 따른 연구의 결과는 다음과 같다. 첫째, 자연친화적 손끝놀이 프로그램이 유아의 전체 소근육 운동기능 발달과 정확성, 신속성, 안정성의 3가지 하위요소의 발달에 긍정적인 영향을 미치는 것을 알 수 있다. 둘째, 자연친화적 손끝놀이프로그램이 유아의 전체 창의적 사고력 및 창의적 사고력 하위요소 중 '유창성', '성급한 종결에 대한 저항'에 긍정적인 영향을 미치는 것을 알 수 있다. 결론적으로 자연친화적 손끝놀이 프로그램이 유아의 소근육 운동기능 발달과 창의적 사고력에 긍정적인 영향을 미치는 것으로 나타났다.

• Journal of Animal Science and Technology
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• 제50권3호
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• pp.293-300
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• 2008

비육 기간 중 근육 내 지방 축적 및 관련 유전자 발현 양상을 확인하기 위하여, 등심 내 지방 및 중성지방 함량을 측정하고 중성지방 생합성 관련 유전자인 ACSS2, GPAT, MGAT 및 DGAT의 유전자 발현량을 real-time PCR법을 통하여 분석하였다. 근육 내 중성지방 함량은 20개월령 이후 급속히 증가됨을 보였고, 중성지방 함량의 증가와 함께 ACSS2, GPAT 및 DGAT 유전자의 발현이 증가됨을 확인하였다. 특히, 12개월령과 비교하여 ACSS2 유전자의 발현은 비육후기인 27개월령에서 7.3배, GPAT는 6.9배 증가되었다. 이러한 중성지방 함량의 증가와 중성지방 생합성 관련 유전자의 발현량 증가는 비육후기에 있어 고 에너지 사료 급여로 인한 지방산의 증가와, 증가된 지방산의 이용성 증대로 축적 형태인 중성지방 합성이 증가되는 것으로 판단되어 진다. 또한 중성지방 생합성 관련 유전자인 ACSS2, GPAT 및 DGAT 유전자의 발현증가는 근육 내 지방 축적과 밀접히 관련되어 있으며, 근내지방 축적과 관련되어 주요한 후보 유전자로 생각되어 진다.

• 한국수산과학회지
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• 제44권6호
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• pp.577-583
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• 2011

In 2007, pet food contaminated with melamine caused hundreds of dogs and cats to develop renal failure all over the world. In 2008, over 294,000 infants consumed infant formula and developed kidney stones in China. Further investigation revealed that fish feed also contained melamine; this raised concerns about melamine residues in edible fish tissues, which could have caused the urinary tract stone epidemic. In Korea, catfish fed with assorted feed that included cuttlefish organs that contained melamine developed whitening syndrome and fell dead in some populations in 2008. This event raised suspicions about the toxicity of melamine and all feeds containing melamine were immediately recalled. In this study, we investigated the rates of melamine accumulation and depletion in muscle and viscera of catfish to propose proper withdrawal periods. One group of catfish was fed a commercially available diet that contained 30, 100 and 300 mg melamine per kg diet for 14 days. To investigate residual melamine contents in muscle and viscera, other experimental groups were fed a melamine free diet after being fed melamine for 7 days. The residual amount of melamine was analyzed by LC-MS/MS. The melamine concentration in muscle was estimated to be 3.7 mg/kg after 6 days of feeding with a diet containing 300 mg melamine/kg. After 2 days of culture with a melamine free diet, the residual melamine was depleted and the concentration had decreased from 1.15 mg/kg to 0.19 mg/kg in the muscle of catfish fed a diet containing 300 mg melamine/kg for 7 days. The residual amount of melamine was reduced to 0.03 mg/kg in muscle after 7 days of culture with a melamine free diet and was undetectable after a prolonged culture period of 14 days. Catfish tend to excrete melamine rapidly after oral administration and changes in body color were not observed during the short dosing period.

• 대한의용생체공학회:의공학회지
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• 제15권4호
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• pp.429-438
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• 1994

An electrical stimulator was designed to induce locomotion for paraplegic patients caused by central nervous system injury. Optimal stimulus parameters, which can minimize muscle fatigue and can achieve effective muscle contraction were determined in slow and fast muscles in Sprague-Dawley rats. Stimulus patterns of our stimulator were designed to simulate eleclromyographic activity monitored during locomotion of normal subjects. Muscle types of the lower extremity were classified according to their mechanical property of contraction, which are slow muscle (msoleus m.) and fast muscle (medial gastrocnemius m., rectus femoris m., vastus lateralis m.). Optimal parameters of electrical stimulation for slow muscles were 20 Hz, 0.2 ms square pulse. For fast muscle, 40 Hz, 0.3 ms square pulse was optimal to produce repeated contraction. Higher'stimulus intensity was required when synergistic muscles were stimulated simultaneously than when they were stimulated individually. Electrical stimulation for each muscle was designed to generate bipedal locomotion, so that individual muscles alternate contraction and relaxation to simulate stance and swing phases. Portable electrical stimulator with 16 channels built in microprocessor was constructed and applied to paraplegic patients due to lumbar cord injury. The electrical slimulator restored partially gait function in paraplegic patients.

• 대한의생명과학회지
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• 제18권1호
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• pp.49-55
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• 2012

Numerous biochemical molecules have been implicated in the development of muscular atrophy. However, control mechanisms associated with muscular disease are not clear. The present study was conducted to investigate gene expression profiles of rat muscle during the denervation to atrophy transition processes. We isolated total RNA from rats suffering from partial muscle atrophy (P) and electromyostimulated atrophy (PE) and synthesized cDNA using annealing control primers. Using 20 ACPs for PCR, we cloned 18 DEGs using TOPO TA cloning vector, sequenced, and analyzed their identities using BLAST search. Sequences of 14 clones significantly matched database entries, while one clone was ESTs, and 3 clones were unidentified. Different expression profiles of selected DEGs between P and PE were confirmed. The troponin T, Fkbp1a, RGD1307554, Phtf1, Atp1a1 and Commd3 were highly expressed genes in the P and PE groups, while Krox-25 and TCOX2 were only expressed genes in the P group, the Sv2b and Marcks were only expressed genes in PE group. also, Cox8h was highly expressed genes in PE groups. The ASPH, ND1, and ARPL1 were highly expressed genes in the P and PE groups. List of genes obtained from the present study might provide an insight for the study of mechanism regulating muscle atrophy and electrostimulated muscle atrophy transitions. These data suggest that troponin T, Fkbp1a, RGD1307554, Phtf1, Atp1a1, and Commd3 are potentially useful as clinical biomarkers of age-related muscle atrophy and dysfunction.

• 한국소음진동공학회논문집
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• 제22권6호
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• pp.502-508
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• 2012

An analytical model for a human body is important to predict muscle and joint forces. Because it is difficult to estimate muscle or joint forces from a human body, the objective of this study is the development of a reliable analytical model for a human body to evaluate the lower extremity muscle and joint forces. The musculoskeletal system of the human lower extremity is modeled as a multibody system employing the Hill-type muscle model. Muscle forces are determined to minimize energy consumption, and we assume that motion is constrained in the sagittal plane. Muscle forces are calculated through an equilibrium analysis while rising from a seated position. The musculoskeletal model consists of four segments. Each segment is a rigid body and connected by frictionless revolute joints. Muscles of the lower extremity are simplified to seven muscles with those that are not related to the sagittal plane motion are ignored. Muscles that play a similar role are combined together. The results of the present study are compared with experimental results to validate the lower extremity model and the assumptions of the present study.

• Asian-Australasian Journal of Animal Sciences
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• 제28권8호
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• pp.1171-1177
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• 2015

The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.

• The Journal of Korean Physical Therapy
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• 제25권2호
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• pp.56-63
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• 2013

Purpose: This study was conducted in order to examine how an effective rehabilitation exercise program influences the activity of shoulder muscles, and to help the clinical application of a rehabilitation program, for prevention and relief of pain, adhesion, and joint stiffness of patients who undergo rotator cuff repair. Methods: Nine test subjects were placed randomly into each group for a total of 27 subjects and exercise program interventions according to the group were conducted for six weeks, after which maximum voluntary isometric contraction (%MVIC) value was re-measured for supraspinatus muscle, infraspinatus muscle, serratus anterior muscle, and middle deltoid muscle in all groups in order to compare changes in muscle activity before and after the experiment in order to perform comparative analysis of changes in muscle activity between groups, based on which four experimental hypotheses were confirmed. Results: Changes in muscle activity according to %MVIC showed a statistically significant difference (p<0.01) (p<0.001) in all muscles, except the middle deltoid muscle, and post-verification results showed that changes in muscle activity according to %MVIC were greater in test groups I and II, compared with the control group, for the supraspinatus muscle, infraspinatus muscle, and serratus anterior muscle. Conclusion: Therefore, rehabilitation through use of the methods described above should be applied efficiently in clinical settings and more research in development of much more efficient rehabilitation program interventions must be conducted.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2005년도 제20차 학술대회 논문집
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• pp.51-51
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• 2005

We inestigated the reproductive cycle with ovarian development of Chlamys farreri by histological observations, and seasonal changes in biochemical components of the adductor muscle, digestive diverticula and ovary were studied by biochemical analysis. The reproductive cycle of this species can be classified into five successive stages: early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (June to August) and spent/inactive stage (August to January). According to ANOVA test, there were significant differences (p<0.05) in total protein, total lipid and glycogen contents among months for all of the adductor muscle, digestive diverticula and ovary. Total protein contents in ovary and digestive diverticula showed significant changes(ANOVA, p<0.05) during the study period, while that in the adductor muscle did not. Total protein content was highest in the adductor muscle, followed by ovary, and lowest in digestive diverticula. There was no correlation in total protein content between the adductor muscle and digestive diverticula (p=0.220). But strong positive correlation was found between adductor muscle and ovary (r=0.450, p=0.013). ANOVA showed that there were significant differences in total lipid and glycogen contents among months for all of the adductor muscle, ovary, and digestive diverticula (p<0.05). The monthly changes in total lipid content were highly variable in ovary and digestive gland. High contents of total lipid were foung during April and May-June in ovary, while March and June-July in digestive diverticula. There was a strong negative correlation in total lipid content between ovary and digestive diverticula (r=- 0.397, p=0.030). Unlike total protein of total lipid, glycogen content in the adductor muscle was most dynamic. It showed more than 36-fold changes in the adductor muscle (at most 3-fold change in ovary) during the study period. Glycogen content was higher during May-July in the adductor muscle, While it was higher in March and august in digestive diverticula. There was a strong negative correlation in glycogen content between the adductor muscle and digestive diverticula (r=-0.584, p=0.001).