• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.242.1-242.1
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• 2002

H$_2$O$_2$ has been shown to act as a signaling molecule involved in many cellular functions such as oxidant-induced stress, apoptosis, proliferation. In this study, we investigated the action mechanisms of H$_2$O$_2$ on activation of Extracellular Signal-Regulated Protein Kinase(ERK) in cultured feline ileal smooth muscle cells(ISMC). Western blot analysis done with phospho-specific MAP kinases antibodies demonstrated that potent activation of ERK and moderate activation of SAPK/JNK occurred within 30 min of H$_2$O$_2$ treatment. (omitted)

• International Journal of Oral Biology
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• 제44권1호
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• pp.20-26
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• 2019

Periodontal diseases have been associated with the development of cardiovascular diseases. Accumulating evidences have indicated that Porphyromonas gingivalis, a major periodontopathic pathogen, plays a critical role in the pathogenesis of atherosclerosis. In the present study, we demonstrated that P. gingivalis lipopolysaccharide (LPS) increases the mRNA and protein expression of matrix metalloproteinase-9 (MMP-9) in rat vascular smooth muscle cells. We showed that the MMP-9 expression induced by P. gingivalis LPS is mediated by the activation of signal transducer and activator of transcription 3 (STAT3) in vascular smooth muscle cells. Furthermore, the inhibition of STAT3 activity reduced P. gingivalis LPS-induced migration of vascular smooth muscle cells. Overall, our findings indicate that P. gingivalis LPS stimulates the migration of vascular smooth muscle cells via STAT3-mediated MMP-9 expression.

• 전기학회논문지
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• 제67권7호
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• pp.910-916
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• 2018

In this paper, we developed a shoulder rehabilitation exercise device and monitoring system to remotely provide rehabilitation system for the ones who need shoulder exercises including the patients with rotator cuff rupture. In order to evaluate the severity of shoulder muscle injury, a total of 4 shoulder rehabilitation exercises ((3) shoulder abduction, (2) shoulder flexion, (3) shoulder abduction with elbow flexion, (4) shoulder extension with elbow flexion) were selected and instructed to be performed with a 3 kg dumbbell for 5 times. For EMG (electromyogram) signal analysis, each subject's maximum voluntary contraction (MVC) was measured. EMG signals reflect the activation level of contracting muscles during dynamic exercises. Six participants' muscle activation levels in posterior deltoid, middle deltoid, upper trapezius, and infraspinatus were measured and compared. The mean power spectrum values in the time and frequency domains were compared between two age-matched groups (20s and 50s). The results showed lower muscle activation in the elderly subjects (n=3) compared to that of the ones in their twenties (n=3).

• 감성과학
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• 제26권4호
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• pp.41-50
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• 2023

본 연구는 EMG(electromyography) 텍스타일 전극 개발을 목적으로 레이어 수의 디자인 및 원단을 다르게 하여 성능 및 신호 획득 안정성을 평가한다. 레이징 및 프레스 공정을 통하여 텍스타일 전극을 제조하며 Layer-0, Layer-1, Layer-2로 레이어 유무 및 수에 따른 결과를 분석했다. 이에 레이어 유무에 따라서는 근활성 측정에 영향을, 수가 많을수록 높은 성능이 나타남을 확인할 수 있었다. Layer-2 구조로 통일하여 5가지의 원단(네오프렌, 스판덱스 쿠션, 폴리에스테르 100%, 나일론 스판덱스, 광목 캔버스)으로 전극을 제조해 실험해 보았다. 성능적인 면에서, 원단의 중량이 높은 나일론 스판덱스가 높은 성능을 보였으며, 스판쿠션 텍스타일 전극이 근활성도 수득에 높은 안정성을 보였다. 이에 위 연구는 레이어에 따른 성능 연관성과 전극-피부사이의 닿는 면적 간의 관계 등을 고찰하여 슬리브 전체의 의복압을 늘리는 대신 특정 센서 측정 부위에만 높은 압력을 가함으로 차후 연구에서 레이어의 수 및 물성에 따른 전극의 공학적 설계 가능성을 제시한 의의가 있다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 춘계학술대회 논문집
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• pp.757-758
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• 2010

• 디지털융복합연구
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• 제18권2호
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• pp.483-487
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• 2020

본 연구는 지면의 유형에 따른 서스펜션 트레이닝의 효과를 분석하기 위해 14명의 건강한 남자 대학생을 대상으로 2종류의 다른 지면 편평한 지면, 짐볼을 이용한 불안정한 지면에 따른 서스펜션 트레이닝의 주목적인 코어 근육의 활성 특성을 연구하였다. 지면의 유형에 따른 코어 근육의 활성도를 측정하기 위해 EMG(Electromyography)를 활용하였고 측정부위는 복직근, 외복사근, 내복사근, 하부요추 기립근의 근활성도를 측정하였다. 측정변인은 각각의 코어 근육 별로 지면의 유형에 따라 근전도 신호를 표준화하기 위해 %MVC방법으로 측정하였다. 지면의 유형에 따른 코어 근육의 차이를 검증하기 위해 paired t-test를 실시하였으며 유의수준은 p<. 05로 설정하였다. 다양한 유형의 지면에 따른 코어 근육의 활성도에 관한 특성을 측정한 결과 두 지면 사이의 근육 별, 지면 별 차이점은 통계적으로 유의하게 나타나지 않았다. 하지만 이는 코어 근육의 트레이닝의 적용 시 반드시 불안정한 형태의 지면에서의 트레이닝 효과가 안정된 지면에서의 효과보다 우월하다는 통념을 다시 한 번 생각하게 하는 중요한 결과라 사료된다. 본 연구의 의미는 불안정한 지면이나 안정된 지면이 코어 근육 활성화에 큰 영향을 미치지 않으므로 어떠한 운동프로그램이던지 정규화된 코어근육 강화프로그램을 실시해도 근육의 활성화에 차이가 없음을 의미한다.

• The Korean Journal of Pain
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• 제34권1호
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• pp.19-26
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• 2021

Background: Prolotherapy is a proliferation therapy as an alternative medicine. A combination of dextrose solution and lidocaine is usually used in prolotherapy. The concentrations of dextrose and lidocaine used in the clinical field are very high (dextrose 10%-25%, lidocaine 0.075%-1%). Several studies show about 1% dextrose and more than 0.2% lidocaine induced cell death in various cell types. We investigated the effects of low concentrations of dextrose and lidocaine in fibroblasts and suggest the optimal range of concentrations of dextrose and lidocaine in prolotherapy. Methods: Various concentrations of dextrose and lidocaine were treated in NIH-3T3. Viability was examined with trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Migration assay was performed for measuring the motile activity. Extracellular signal-regulated kinase (Erk) activation and protein expression of collagen I and α-smooth muscle actin (α-SMA) were determined with western blot analysis. Results: The cell viability was decreased in concentrations of more than 5% dextrose and 0.1% lidocaine. However, in the concentrations 1% dextrose (D1) and 0.01% lidocaine (L0.01), fibroblasts proliferated mildly. The ability of migration in fibroblast was increased in the D1, L0.01, and D1 + L0.01 groups sequentially. D1 and L0.01 increased Erk activation and the expression of collagen I and α-SMA and D1 + L0.01 further increased. The inhibition of Erk activation suppressed fibroblast proliferation and the synthesis of collagen I. Conclusions: D1, L0.01, and the combination of D1 and L0.01 induced fibroblast proliferation and increased collagen I synthesis via Erk activation.

• 대한인간공학회지
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• 제30권6호
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• pp.689-694
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• 2011

Objective: The aim of this study is to evaluate the fluctuation of signal amplitude during repetitive dynamic contraction based on surface electromyography(EMG). Background: The most previous studies were considered isometric muscle contraction and they were difference to smoothing window length by moving average filter. In practical, the human movement is dynamic state. Dynamic EMG signal which indicated as the nonstationary pattern should be analyzed differently compared with the static EMG signal. Method: Ten male subjects participated in this experiment, and EMG signal was recorded by biceps brachii, anterior/posterior deltoid, and upper/lower trapezius muscles. The subject was performed to repetitive right horizontal lifting task during ten cycles. This study was considered three independent variables(muscle, amplitude processing technique, and smoothing window length) as the within-subject experimental design. This study was estimated muscular activation by means of the linear envelope technique(LE). The dependent variable was set coefficient of variation(CV) of LE for each cycle. Results: The ANOVA results showed that the main and interaction effects between the amplitude processing technique and smoothing window length were significant difference. The CV value of peak LE was higher than mean LE. According to increase the smoothing window length, this study shows that the CV trend of peak LE was decreased. However, the CV of mean LE was analyzed constant fluctuation trend regardless of the smoothing window length. Conclusion: Based on these results, we expected that using the mean LE and 300ms window length increased reproducibility and signal noise ratio during repetitive dynamic muscle contraction. Application: These results can be used to provide fundamental information for repetitive dynamic EMG signal processing.

• 한국전문물리치료학회지
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• 제30권3호
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• pp.221-229
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• 2023

Background: In modern society, the use of computers accounts for a large proportion of our daily lives. Although substantial research is being actively conducted on musculoskeletal diseases resulting from computer use, there has been a recent surge in interest in improving the working environment for prevention. Objects: This study aimed to examine the effects of posture correction feedback (PCF) on changes in neck posture and muscle activation during computer typing. Methods: The participants performed a computer typing task in two sessions, each lasting 16 minutes. The participant's dominant side was photographed and analyzed using ImageJ software to verify neck posture. Surface electromyography (EMG) was used to confirm the participant's cervical erector spinae (CES) and upper trapezius muscle activities. The EMG signal was analyzed using the percentage of reference voluntary contraction and amplitude probability distribution function (APDF). In the second session, visual and auditory feedback for posture correction was provided if the neck was flexed by more than 15° in the initial position during computer typing. A 20-minute rest period was provided between the two sessions. Results: The neck angle (p = 0.014), CES muscle activity (p = 0.008), and APDF (p = 0.015) showed significant differences depending on the presence of the PCF. Furthermore, significant differences were observed regarding the CES muscle activity (p = 0.001) and APDF (p = 0.002) over time. Conclusion: Our study showed that the feedback system can correct poor posture and reduces unnecessary muscle activation during computer work. The improved neck posture and reduced CES muscle activity observed in this study suggest that neck pain can be prevented. Based on these results, we suggest that the PCF system can be used to prevent neck pain.

• The Korean Journal of Physiology and Pharmacology
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• 제5권4호
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• pp.287-297
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• 2001

Contraction of smooth muscle is initiated by an increase in cytosolic $Ca^{2+}$ leading to activation of $Ca^{2+}$/ calmodulin-dependnet myosin light chain (MLC) kinase and phosphorylation of MLC. The types of contraction and signaling mechanisms mediating contraction differ depending on the region. The involvement of these different mechanisms varies depending on the source of $Ca^{2+}$ and the kinetic of $Ca^{2+}$ mobilization. $Ca^{2+}$ mobilizing agonists stimulate different phospholipases $(PLC-{\beta},\;PLD\;and\;PLA_2)$ to generate one or more $Ca^{2+}$ mobilizing messengers $(IP_3\;and\;AA),$ and diacylglycerol (DAG), an activator of protein kinase C (PKC). The relative contributions of $PLC-{\beta},\;PLA_2$ and PLD to generate second messengers vary greatly between cells and types of contraction. In smooth muscle cell derived form the circular muscle layer of the intestine, preferential hydrolysis of $PIP_2$ and generation of $IP_3$ and $IP_3-dependent\;Ca^{2+}$ release initiate the contraction. In smooth muscle cells derived from longitudinal muscle layer of the intestine, preferential hydrolysis of PC by PLA2, generation of AA and AA-mediated $Ca^{2+}$ influx, cADP ribose formation and $Ca^{2+}-induced\;Ca^{2+}$ release initiate the contraction. Sustained contraction, however, in both cell types is mediated by $Ca^{2+}-independent$ mechanism involving activation of $PKC-{\varepsilon}$ by DAG derived form PLD. A functional linkage between $G_{13},$ RhoA, ROCK, $PKC-{\varepsilon},$ CPI-17 and MLC phosphorylation in sustained contraction has been implicated. Contraction of normal esophageal circular muscle (ESO) in response to acetylcholine (ACh) is linked to $M_2$ muscarinic receptors activating at least three intracellular phospholipases, i.e. phosphatidylcholine-specific phospholipase C (PC-PLC), phospholipase D (PLD) and the high molecular weight (85 kDa) cytosolic phospholipase $A_2\;(cPLA_2)$ to induce phosphatidylcholine (PC) metabolism, production of diacylglycerol (DAG) and arachidonic acid (AA), resulting in activation of a protein kinase C (PKC)-dependent pathway. In contrast, lower esophageal sphincter (LES) contraction induced by maximally effective doses of ACh is mediated by muscarinic $M_3$ receptors, linked to pertussis toxin-insensitive GTP-binding proteins of the $G_{q/11}$ type. They activate phospholipase C, which hydrolyzes phosphatidylinositol bisphosphate $(PIP_2),$ producing inositol 1, 4, 5-trisphosphate $(IP_3)$ and DAG. $IP_3$ causes release of intracellular $Ca^{2+}$ and formation of a $Ca^{2+}$-calmodulin complex, resulting in activation of myosin light chain kinase and contraction through a calmodulin-dependent pathway.