• Title/Summary/Keyword: Murine NBT-II cells

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Apoptosis of 4-Acetyl-12, 13-epoxyl-9-trichothecene-3, 15-diol isolated from the fruiting bodies of Isaria japonica Yasuda via Caspase-3 in Bladder cancer Cell line (NBT-II) (Caspase-3을 경유한 동충하초 자실체 유래 4-Acetyl-12, 13-epoxyl-9-trichothecene-3, 15-diol의 방광암 세포주(NBT-II) Apoptosis)

  • Kim Hyeong-Jin;Jang S.I.;Oh K,S.;Hong K.H.;Kim Y.C.;Pae H.O.;Yun Y.G.;Chung H.T.;Kwon T.O.
    • Herbal Formula Science
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    • v.10 no.2
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    • pp.213-223
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    • 2002

  • The fruiting bodies of Isaria japonica have been traditionally used in Korea to treat cancer. An apoptosis-inducing compound, 4-Acetyl-12, 13-epoxyl -9-trichothecene-3, I5-diol (AETD), was isolated from the methanol extract of fruiting bodies of Isaria japonica Yasuda by bioassay -guided fractionation. The apoptosis of murine bladder cancer cell line (NBT-Ⅱ) by the compound was accessed by propidium iodide staining flow cytometric analysis, and apoptosis-inducing activity at $IC_{50}$ concentration (5 nmol／L) was further confirmed by a nuclear morphological change, a ladder pattern of DNA fragmentation, and an activation of caspase-3. These results indicate that AETD induces apoptosis of NBT-Ⅱ cells via expression of caspase-3.

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