• Title/Summary/Keyword: Multivariate structure

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Metagenomic Analysis of Jang Using Next-generation Sequencing: A ComparativeMicrobial Study of Korean Traditional Fermented Soybean Foods (차세대 염기서열 분석을 활용한 장류의 메타지놈 분석 : 한국 전통 콩 발효식품에 대한 미생물 비교 연구)

  • Ranhee Lee;Gwangsu Ha;Ho Jin Jeong;Do-Youn Jeong;Hee-Jong Yang
    • Journal of Life Science
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    • v.34 no.4
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    • pp.254-263
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    • 2024
  • Korean jang is a food made using fermented soybeans, and the typical products include gochujang (GO), doenjang (DO), cheonggukjang (CH), and ganjang (GA). In this study, 16S rRNA metagenome analysis was performed on a total of 200 types of GO, DO, CH, and GA using next-generation sequencing to analyze the microbial community of fermented soybean foods and compare taxonomic (biomarker) differences. Alpha diversity analysis showed that in the CHAO index, the species richness index tended to be significantly higher compared to the DO and GA groups (p<0.001). The results of the microbial distribution analysis of the GO, DO, CH, and GA products showed that at the order level, Bacillales was the most abundant in the GO, DO, and CH groups, but Lactobacillales was most abundant in the GA group. Linear discriminant analysis effect (LEfSe) analysis was used to identify biomarkers at the family and species levels. Leuconostocaceae, Thermoactinomycetaceae, Bacillaceae, and Enterococcaceae appeared as biomarkers at the family level, and Bacillus subtilis, Kroppenstedtia sanguinis, Bacillus licheniformis, and Tetragenococcus halophilus appeared at the species level. Permutational multivariate analysis of variance (PERMANOVA) analysis showed that there was a significant difference in the microbial community structure of the GO, DO, CH, and GA groups (p=0.001), and the microbial community structure of the GA group showed the greatest difference. This study clarified the correlation between the characteristics of Korean fermented foods and microbial community distribution, enhancing knowledge of microorganisms participating in the fermentation process. These results could be leveraged to improve the quality of fermented soybean foods.

Fault Detection & SPC of Batch Process using Multi-way Regression Method (다축-다변량회귀분석 기법을 이용한 회분식 공정의 이상감지 및 통계적 제어 방법)

  • Woo, Kyoung Sup;Lee, Chang Jun;Han, Kyoung Hoon;Ko, Jae Wook;Yoon, En Sup
    • Korean Chemical Engineering Research
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    • v.45 no.1
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    • pp.32-38
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    • 2007
  • A batch Process has a multi-way data structure that consists of batch-time-variable axis, so the statistical modeling of a batch process is a difficult and challenging issue to the process engineers. In this study, We applied a statistical process control technique to the general batch process data, and implemented a fault-detection and Statistical process control system that was able to detect, identify and diagnose the fault. Semiconductor etch process and semi-batch styrene-butadiene rubber process data are used to case study. Before the modeling, we pre-processed the data using the multi-way unfolding technique to decompose the data structure. Multivariate regression techniques like support vector regression and partial least squares were used to identify the relation between the process variables and process condition. Finally, we constructed the root mean squared error chart and variable contribution chart to diagnose the faults.

Analysis of extreme wind speed and precipitation using copula (코플라함수를 이용한 극단치 강풍과 강수 분석)

  • Kwon, Taeyong;Yoon, Sanghoo
    • Journal of the Korean Data and Information Science Society
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    • v.28 no.4
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    • pp.797-810
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    • 2017
  • The Korean peninsula is exposed to typhoons every year. Typhoons cause huge socioeconomic damage because tropical cyclones tend to occur with strong winds and heavy precipitation. In order to understand the complex dependence structure between strong winds and heavy precipitation, the copula links a set of univariate distributions to a multivariate distribution and has been actively studied in the field of hydrology. In this study, we carried out analysis using data of wind speed and precipitation collected from the weather stations in Busan and Jeju. Log-Normal, Gamma, and Weibull distributions were considered to explain marginal distributions of the copula. Kolmogorov-Smirnov, Cramer-von-Mises, and Anderson-Darling test statistics were employed for testing the goodness-of-fit of marginal distribution. Observed pseudo data were calculated through inverse transformation method for establishing the copula. Elliptical, archimedean, and extreme copula were considered to explain the dependence structure between strong winds and heavy precipitation. In selecting the best copula, we employed the Cramer-von-Mises test and cross-validation. In Busan, precipitation according to average wind speed followed t copula and precipitation just as maximum wind speed adopted Clayton copula. In Jeju, precipitation according to maximum wind speed complied Normal copula and average wind speed as stated in precipitation followed Frank copula and maximum wind speed according to precipitation observed Husler-Reiss copula.

Development of Time-Dependent Reliability-Based Design Method Based on Stochastic Process on Caisson Sliding of Vertical Breakwater (직립방파제의 케이슨 활동에 대한 확률과정에 기반한 시간의존 신뢰성 설계법 개발)

  • Kim, Seung-Woo;Cheon, Sehyeon;Suh, Kyung-Duck
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.24 no.5
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    • pp.305-318
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    • 2012
  • Although the existing performance-based design method for the vertical breakwater evaluates an average sliding distance during an arbitrary time, it does not calculate the probability of the first occurrence of an event exceeding an allowable sliding distance(i.e. the first-passage probability). Designers need information about the probability that the structure is damaged for the first time for not only design but also maintenance and operation of the structure. Therefore, in this study, a time-dependent reliability design method based on a stochastic process is developed to evaluate the first-passage probability of caisson sliding. Caisson sliding can be formulated by the Poisson spike process because both occurrence time and intensity of severe waves causing caisson sliding are random processes. The occurrence rate of severe waves is expressed as a function of the distribution function of sliding distance and mean occurrence rate of severe waves. These values simulated by a performance-based design method are expressed as multivariate regression functions of design variables. As a result, because the distribution function of sliding distance and the mean occurrence rate of severe waves are expressed as functions of significant wave height, caisson width, and water depth, the first-passage probability of caisson sliding can be easily evaluated.

Relationship between Status Type of Sociometric Measurement and Korean Personality Test for Children (사회성측정 지위 유형과 한국 아동 성격검사와의 관계)

  • Lee, Meung-Sook;Ahn, Ie-Hwan;Hong, Sang-Hwang
    • The Korean Journal of Elementary Counseling
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    • v.11 no.3
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    • pp.391-405
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    • 2012
  • The purpose of this study is to discover unique personality traits which the children of 5 social status types using 10 clinical scales of Korea Children's personality test (KCPI-S) in order to define the characteristics of 5 social status types developed as the Korean type. For this purpose, two hypotheses were set and tested. The subjects of this study were 502 children who were 4, 5th and 6th grade elementary school boys and girls in A city. 5 social status types and gender of children were set as the independent variables each, and 10 clinical scales of Korea Children's personality test as the dependent variables. The research hypotheses were analyzed in turn by the multivariate analysis (MANOVA) and t-test. The results are as follows: First, the results of analyzing differences in 10 clinical scales in accordance with 5 social status types how the significant differences in all 9 clinical scales except for language development scale, and ${\eta}^2$ representing the explanatory power of the independent variables to dependent variables was found to be in the range of the lowest at 2% up to 10% for each scale. Second, the results of analysis of the gender difference show that in the case of the children with controversial and neglected children, the significant difference was appeared between boys and girls in 8-9 scales among the 10 clinical scales, but in the case of rest 3 social status types (average, popular, rejected children) the difference was appeared between boys and girls only in 2-3 scales. Depending on these results it seems that each of 5 social status types of the children has its own unique personality structure. It is necessary to attend to the fact that in the case of the children with controversial and neglected children, the average value of boys are significantly higher than that of the girls. There is a big possibility that the boys have more psychological problems that the girls. It is judged that in case of these two types the personal traits applying gender should be interpreted. Therefore, the follow-up study should conduct the exploratory research on the structure of the relationship between children's language development and sociality measure. Further, it is necessary to conduct the psychological comparison between genders in the children with controversial and neglected children, and to study the structure of their relationship with rest social status types by genders.

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Community Structure and Health Assessment of Macrobenthic Assemblages at Spring and Summer in Geoje-Hansan Bay, Southern Coast of Korea (거제한산만에 서식하는 대형저서동물의 춘계와 하계의 군집구조 및 건강도 평가)

  • Jung, Rae Hong;Seo, In-Soo;Choi, Byoung-Mi;Choi, Minkyu;Yoon, Sang-Pil;Park, Sang Rul;Na, Jong Hun;Yun, Jae Seong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.1
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    • pp.27-41
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    • 2016
  • This study was performed to investigate the community structure and health assessment of macrobenthic assemblages in Geoje-Hansan Bay, Southern Coast of Korea. Macrobenthos were collected by van Veen grab sampler at May (spring) and August (summer) 2013. The total species number and mean density were 300 species $7.6m^{-2}$ and $1,994ind.\;m^{-2}$, respectively. The major dominant species were the polychaetes Lumbrineris longifolia ($299{\pm}164ind.\;m^{-2}$), Aphelochaeta monilaris ($100{\pm}57ind.\;m^{-2}$), the bivalve Musculista senhousia ($91{\pm}96ind.\;m^{-2}$) and the polychaete Praxillella affinis ($80{\pm}66ind.\;m^{-2}$). From the community statistics [cluster analysis and nonmetric multidimentional scaling (NMDS) ordination], the macrobenthic community was distinguished into two groups of inner bay (farming ground of near Sandal Island) and channel station(from Hansan Island to Chubong Island) group. In this study, the ecological status was assessed by four biotic indices Shannon's H', the ATZI Marine Biotic Index (AMBI), multivariate-AMBI (M-AMBI) and the Environment Conservation Index (ECI). The ecological status of the macrobenthic community in Geoje-Hansan Bay were poorer in the inner bay station than in the channel station. The results of the present study showed that three biotic indices (Shannon's H', M-AMBI and ECI) were valid as an index for evaluating the ecological status than the AMBI.

Spatio-temporal Changes in Macrobenthic Community Structure and Benthic Environment at an Intensive Oyster Culturing Ground in Geoje-Hansan Bay, Korea (굴 양식장 밀집해역인 거제한산만의 저서동물군집 구조와 저서환경의 시.공간적 변동)

  • Yoon, Sang-Pil;Jung, Rae-Hong;Kim, Youn-Jung;Hong, Sok-Jin;Oh, Hyun-Taik;Lee, Won-Chan
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.14 no.4
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    • pp.213-228
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    • 2009
  • This study was conducted to investigate spatio-temporal changes in macrobenthic community structure and benthic environmental conditions in Geoje-Hansa Bay, which is the greatest oyster producing site in Korea. Field survey for benthic environment and macrobenthos was seasonally carried out at 15 stations covering oyster farming sites and non-farming sites from February to November, 2008. The grain size of surface sediments was dominated by very fine silt with the mean phi of about $9\;{\Phi}$ and TOC was 1.9% on average. Mean dissolved oxygen content was 8.1 mg/L and lowest in August corresponding to the 2nd degree in seawater quality criteria. Total species number was 351 and mean density was $3,675\;ind./m^2$, both of which were dominated by polychaete worms. Spatio-temporal variation in above two biological variables was great with higher values seasonally in spring and spatially in channels rather than inner bay. Dominant species were Lumbrineris longifolia (21.3%), Aphelochaeta monilaris (17.8%) and Ericthonius pugnax(6.1%), all of which are typical species of organically enriched area. From the multivariate analyses, the whole macrobenthic community was distinguished into two groups of channel and inner bay group. Spatio-temporal changes of macrobenthic community in Geoje-Hansan Bay were related to those of TOC and acid volatile sulfide (AVS). Our results showed that Geoje-Hansan Bay should be intermediately affected by organic pollution, and that such organic enrichment was more remarkable at farming stations in the inner bay.

Community Structure of Macrobenthic Fauna in the Hallyeohaesang National Park from Korea Strait, Korea (한려해상국립공원 조하대 해역 대형저서동물의 군집구조)

  • Yoon, Kon-Tak;Seo, In-Soo;Kim, Kwang-Bong;Cho, Byoung-Mi;Son, Min-Ho
    • Korean Journal of Environmental Biology
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    • v.27 no.1
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    • pp.124-133
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    • 2009
  • The study was performed to investigate the community structure of macrobenthic fauna during June 2005, in Hallyeohaesang National Park from Korea Strait. A total of 284 macrobenthic fauna were collected. The overall average macrobenthic density and biomass were 2,002 individuals m$^{-2}$ and 154.92 gWWt m$^{-2}$, respectively. Based on the Lebris (1988) index, there were 20 species accounting for approximately 47.64% of total individuals. And the highest densities were found in the polychaetes Scoletoma longifolia, Isolda pulchella, Mediomastus californiensis, Minuspio multibranchia, Tharyx sp. 1 and the bivalve Theora fragilis. On the other hand, the top twenty species made up 70.47% of the total biomass while the three most abundant, the echinoderms Schizaster lacunosus, Amphiura vadicola and the bivalve Fulvia mutica. The conventional multivariate statistics (cluster analysis and non-metric Multi-Dimensional Scaling) applied to assess spatial variation in macrobenthic assemblages. As a result of cluster analysis and nMDS ordination, this study area was divided into two different groups of stations and species. In case of station, there are two groups: one is a mud dominated station group and the other is dead shell and sand dominated group. And the 2 faunistic groups were established as follows: 1) a group of relatively higher abundances in mud dominated sediment. There were numerically dominated by the polychaetes Magelona japonica, Sternaspis scutata, Paraprionospio pinnata, Tharyx sp. 1 and the amphipods Monocorophium sinense and Eriopisella sechellensis. 2) a group of relatively higher densities dead shell and sand dominated sediment. These groups were characterized by the polychaetes I. pulchella, M. multibranchiata, the amphipods M. acherusicum, Gammaropsis japonicus and the echinoderm A. vadicola.

Fecal Microbiota Profiling of Holstein and Jersey, in South Korea : A Comparative Study (국내에서 사육되는 Holstein 젖소과 Jersey 젖소의 대변 미생물 분석 : 비교연구)

  • Gwangsu Ha;Ji-Won Seo;Hee Gun Yang;Se Won Park;Soo-Young Lee;Young Kyoung Park;RanHee Lee;Do-Youn Jeong;Hee-Jong Yang
    • Journal of Life Science
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    • v.33 no.7
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    • pp.565-573
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    • 2023
  • In light of the complex interactions between the host animal and its resident gut microbiomes, studies of these microbial communities as a means to improve cattle production are important. This study was conducted to analyze the intestinal microorganisms of Holstein (HT) and Jersey (JS), raised in Korea and to clarify the differences in microbial structures according to cattle species through next-generation sequencing. The alpha-diversity analysis revealed that most species richness and diversity indices were significantly higher in JS than in HT whereas phylogenetic diversity, which is the sum of taxonomic distances, is not significant. Microbial composition analysis showed that the intestinal microbial community structure of the two groups differed. In the both groups, a significant correlation was observed among the distribution of several microbes at the family level. In particular, a highly significant correlation (p<0.0001) among a variety of microbial distributions was found in JS. Beta-diversity analyis was to performed to statistically verify whether a difference exists in the intestinal microbial community structure of the two groups. Principal coordinate analysis and unweighted pair group method with arithmetic mean (UPGMA) clustering analysis showed separation between the HT and JS clusters. Meanwhile, permutational multivariate analysis of variance (PERMANOVA) revealed that their microbial structures are significantly different (p<0.0001). LEfSe biomarker analysis was performed to discover the differenc microbial features between the two groups. We found that several microbes, such as Firmicutes, Bacilli, Moraxellaceae and Pseudomonadales account for most of the difference in intestinal microbial community structure between the two groups.

The Perceived Importance Weight of Product Information Cues in E-Shopping (온라인 쇼핑에서 소지자가 지각하는 제품 정보 중요성의 비중)

  • Lee Kyu-Hye;Park Jihye
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.3 s.151
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    • pp.470-480
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    • 2006
  • Consumer may perceive needs of certain product information online rather than the number of pieces of information available for decision making. In addition, consumers may seek information that is more relevant to apparel category. Moreover, involved consumers intensify information seeking and seek certain information. The purpose of this study is to identify the perceived importance weight of each information cue when shopping apparel via the Internet, to investigate the differences of the perceived importance weight of product information cues in product category, and to examine the relationship between apparel involvement and the perceived importance weight of product information cues. This study employed a single-factor within-subjects design experiment that simulated online purchase situation for two product types, Jeans and a Shirt. A total of 125 college students participated in this study. Results indicated that selected information such size available, price, style, color description, item measurement, stock available, and item quality (in sequence) can be considered as global cues to judge product quality and influence purchase decision regardless of product category. The significant multivariate effects for product category on the perceived importance weight of product information cues were found. Personalization, fiber content, and fabric structure were product specific information cues. Consumers' product involvement significantly influenced the perception of information weight. Therefore, product information can be personalized based on consumer involvement