• Genomics & Informatics
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• v.10 no.4
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• pp.249-255
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• 2012

In this study, fosmid cloning strategies were used to assess the microbial populations in water from the International Space Station (ISS) drinking water system (henceforth referred to as Prebiocide and Tank A water samples). The goals of this study were: to compare the sensitivity of the fosmid cloning strategy with that of traditional culture-based and 16S rRNA-based approaches and to detect the widest possible spectrum of microbial populations during the water purification process. Initially, microbes could not be cultivated, and conventional PCR failed to amplify 16S rDNA fragments from these low biomass samples. Therefore, randomly primed rolling-circle amplification was used to amplify any DNA that might be present in the samples, followed by size selection by using pulsed-field gel electrophoresis. The amplified high-molecular- weight DNA from both samples was cloned into fosmid vectors. Several hundred clones were randomly selected for sequencing, followed by Blastn/Blastx searches. Sequences encoding specific genes from Burkholderia, a species abundant in the soil and groundwater, were found in both samples. Bradyrhizobium and Mesorhizobium, which belong to rhizobia, a large community of nitrogen fixers often found in association with plant roots, were present in the Prebiocide samples. Ralstonia, which is prevalent in soils with a high heavy metal content, was detected in the Tank A samples. The detection of many unidentified sequences suggests the presence of potentially novel microbial fingerprints. The bacterial diversity detected in this pilot study using a fosmid vector approach was higher than that detected by conventional 16S rRNA gene sequencing.

• Journal of The Korean Astronomical Society
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• v.47 no.6
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• pp.303-309
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• 2014

We present results of our investigation of the radio intrinsic brightness temperatures of compact radio jets. The intrinsic brightness temperatures of about 100 compact radio jets at 2, 5, 8, 15, and 86 GHz are estimated based on large VLBI surveys conducted in 2001-2003 (or in 1996 for the 5 GHz sample). The multi-frequency intrinsic brightness temperatures of the sample of jets are determined by a statistical method relating the observed brightness temperatures with the maximal apparent jet speeds, assuming one representative intrinsic brightness temperature for a sample of jets at each observing frequency. By investigating the observed brightness temperatures at 15 GHz in multiple epochs, we find that the determination of the intrinsic brightness temperature for our sample is affected by the flux density variability of individual jets at time scales of a few years. This implies that it is important to use contemporaneous VLBI observations for the multi-frequency analysis of intrinsic brightness temperatures. Since our analysis is based on the VLBI observations conducted in 2001-2003, the results are not strongly affected by the flux density variability. We find that the intrinsic brightness temperature $T_0$ increases as $T_0{\propto}{\nu}^{\xi}_{obs}$ with ${\xi}=0.7$ below a critical frequency ${\nu}_c{\approx}9GHz$ where the energy loss begins to dominate the emission. Above ${\nu}_c$, $T_0$ decreases with ${\xi}=-1.2$, supporting the decelerating jet model or particle cascade model. We also find that the peak value of $T_0{\approx}3.4{\times}10^{10}$ K is close to the equipartition temperature, implying that the VLBI cores observable at 2-86 GHz may be representing jet regions where the magnetic field energy dominates the total energy in jets.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2006.11a
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• pp.11-14
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• 2006

This study investigates the flow field of multiple-jet measured by hot-wire anemometry. The experiments were classified into two cases; 6- or 7-nozzle located circumferentially in equal interval without or with a central jet. The effect of the number of nozzles the flow field was examined when the Reynolds number based on the nozzle diameter is about $10^4$. Mean Velocity, normal and Reynolds stresses were measured in the downstream of jets. The Tollmien's theory holds far downstream at 48d apart from the nozzle exit especially when a nozzle locates at the center. The general flow characteristics is influenced due to the number of nozzles.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.57.1-57.1
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• 2014

We present results of our investigation of intrinsic brightness temperatures of compact radio jets at radio frequencies. The intrinsic brightness temperatures of about 100 compact radio jets at 2, 5, 8, 15, and 86 GHz are estimated based on large VLBI surveys conducted in 2001-2003 (or in 1996 for the 5 GHz sample). The multi-freqeuncy intrinsic brightness temperatures of the sample of the jets are determined with a statistical method relating the observed brightness temperatures with the maximal apparent jet speed, assuming one representative intrinsic brightness temperature for the sample at each observing frequency. With investigating the observed brightness temperatures at 15 GHz in multiple epochs, we found that the determination of the intrinsc brightness temperature for our sample is affected by variability of individual jets in flux density at the time scales of a few years. This implies an importance of contemporaneity of the multi-frequency VLBI observations for the statistical method. Since our analysis is based on the VLBI observations conducted in 2001-2003, the results are less affected by the flux density variability. We found that the intrinsic brightness temperature $T_0$ increases as $T_0{\propto}{\nu}^{\epsilon}$ with ${\epsilon}{\approx}0.7$ below a critical frequency ${\nu}_c{\approx}10GHz$ where energy losses begin to dominate the emission, and above the critical frequency, $T_0$ decreases with ${\epsilon}{\approx}-1.2$ supporting for the decelerating jet model.

• 한국전산유체공학회:학술대회논문집
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• 2006.05a
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• pp.395-397
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• 2006

Air interlacing serves to protect the yarn against damage, strengthens inter-filament compactness or cohesion, and ensures fabric consistency. The air interlacing nozzle is used to introduce intermittent nips to a filament yarn so as to improve its performance in textile processing. The effect of various interlacing nozzle geometries on the interlacing process was studied. The geometries of interlacing nozzles with single or multiple air inlets located across the width of yarn channels are investigated. The basis case is the yarn channel, with a perpendicular main air inlet in the middle. Other cases have main air inlets, slightly inclined double sub air inlets, The yarn channel cross sectional shapes are either semicircular or rectangular shapes. The compressed impinging jet from the main air inlet hole hits the opposing bottom wall of the yarn channel, is divided into two branches, joins with the compressed air coming out from sub air inlet at the bottom and creates two free jets at both ends of the yarn channel. The compressed air movement in the cross-section consists of two opposing directional vortices. The CFD-FASTRAN flow parallel solver was used to perform steady simulations of impinging jet flow inside of the interlace nozzles. The vortical structure and the flow pattern such as pressure contour, particle traces, velocity vector plots inside of interlace nozzle geometry are discussed in this pater.

• The Journal of Korean Academy of Prosthodontics
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• v.44 no.6
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• pp.690-698
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• 2006

Statement of problems. The heat produced during polymerization of polymer-based provisional materials may cause thermal damage to the vital pulp. Purpose. This study was performed to evaluate the exotherm reaction of the polymerbased provisional materials during polymerization by differential scanning calorimetry and to compare the temperature changes of different types of resins. Material and methods. Three dimethacrylate-based materials (Protemp 3 Garant, Luxatemp Plus, Luxatemp Fluorescence) and five monomethacrylate- based material (Snap, Alike, Unifast TRAD, Duralay, Jet) were selected. Temperature changes of polymer-based provisional materials during polymerization in this study were evaluated by D.S.C Q-1000 (TA Instrument, Wilmington, DE, USA). The following three measurements were determined from the temperature versus time plot: (1) peak temperature, (2) time to reach peak temperature, (3) heat capacity. The data were statistically analyzed using one-way ANOVA and multiple comparison Bonferroni test at the significance level of 0.05. Results. The mean peak temperature was $39.5^{\circ}C({\pm}\;1.0)$. The peak temperature of the polymer-based provisional materials decreased in the following order: Duralay > Unifast TRAD, Alike > Jet > Luxatemp Plus, Protemp 3 Garant, Snap, Luxatemp Fluorescence. The mean time to reach peak temperature was 95.95 sec $({\pm}\;64.0)$. The mean time to reach peak temperature of the polymer-based provisional materials decreased in the following order: Snap, Jet > Duralay > Alike > Unifast TRAD > Luxatemp Plus, Protemp 3 Garant, Luxatemp Fluorescence. The mean heat capacity was 287.2 J/g $({\pm}\;107.68)$. The heat capacity of the polymer-based provisional materials decreased in the following order: Duralay > TRAD, Jet, Alike > Snap, Luxatemp Fluorescence, Protemp 3 Garant, Luxatemp Plus. Conclusion. The heat capacity of materials, determined by D.S.C., is a factor in determining the thermal insulating properties of restorative materials. The peak temperature of PMMA was significantly higher than others (PEMA, dimethacrylate). No significant differences were found among PEMA (Snap) and dimethacrylate (P >0.05). The time to reach peak temperature was greatest with PEMA, followed by PMMA and dimethacrylate. The heat capacity of PMMA was significantly higher than others (PEMA, dimethacrylate). No significant differences were found among PEMA and dimethacrylate (P >0.05).

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.379-379
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• 2013

본 논문에서는 Normal ink jetting 공법으로 OTFT를 제작할 때 coffee stain effect에 의해서 반도체 소자의 특성이 저하되는 것을 극복하기 위해서 동일한 위치에 동일한 부피로 Droplet을 형성하는 Multiple ink jetting 공법을 통해 TIPS pentacene 결정의 Morphology와 전기적 특성이 어떻게 변화하는지 알아 보았다. Multiple ink jetting의 drop 횟수가 증가할수록 coffee stain effect에 의해서 형성된 가운데 영역의 Dendrite grain이 점점 작아지다가 7 Drops 이후로는 Big grain 만 남게 되었다. Active layer의 표면 Roughness는 drop 횟수가 증가할수록 낮아지다가 일정 count 이후로는 다시 높아지는 것을 확인할 수 있었다. 전계 이동도(mobility)는 drop 횟수가 증가할수록 커지다가 일정 count 이후로는 saturation되는 것을 확인할 수 있었다. Multiple ink jetting에 의해서 만들어진 OTFT 소자의 전계 이동도(mobility)는 1 drop과 10 drops에서 각각 0.0059, 0.036 cm2/Vs 로 6배 정도 차이가 있었다. 이것은 첫 drop에 의해 만들어진 가운데 Dendrite grain 영역이 Multiple ink jetting을 반복하면서 점점 작아지게 되어 사라지고 두꺼운 Grain 영역만 남게 된 것으로 판단된다. Vth 와 On/Off ratio는 1 drop과 10 drops에서 각각 -3 V, -2 V 그리고 $3.3{\times}10^3$, $1.0{\times}10^4$를 보였다. OTFT의 substrate로 Flexible한 polyethersulfone (PES) 기판을 사용하였고, 절연체로 Spin coating된 Poly-4-vinylphenol (PVP)가 사용되었으며, Gate 및 Source/Drain 전극은 Au를 50 nm 두께로 증착하였다. Channel의 width와 length는 각각 100 um, 40 um 였고, Gate 전극 위에 Active layer를 형성한 Bottom gate 구조로 제작되었다. Ink jet으로 제작된 TIPS pentacene의 결정성은 x-ray diffraction (XRD)와 광학 현미경으로 분석하였고 Thickness profile은 알파스텝 측정기를 이용하였으며, OTFT의 전기적 특성은 Keithley-4,200을 사용하여 측정하였다.

• Journal of Advanced Marine Engineering and Technology
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• v.34 no.1
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• pp.84-91
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• 2010

The centrifugal oil purifier is used in ships for purifying the engine lubrication oil. The momentum needed for the rotation of the cylindrical chamber is obtained by jet injections. The dust particles in the oil are separated by the centrifugal forces moving to the inner wall of the rotating cylindrical chamber body. The dust particles are eliminated when the particles are adsorbed onto the surface of the inner wall of the chamber body. The flow characteristics and the physical behaviours of particles in this centrifugal oil purifier have been investigated numerically and the filtration efficiencies have been evaluated. For the calculations, a commercial code has been used and the SST k-${\omega}$ turbulence model has been adopted. The MRF (Multiple Reference Frame) method has been introduced to consider the rotating effect of the flows. Under various variables, such as particle size, particle density and rotating speed, the filtration efficiencies have been evaluated. It has been verified that the filtration efficiency is increased with the increments of the particle size, the particle density and the rotating speed of the cylindrical chamber.

• Environmental Engineering Research
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• v.22 no.1
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• pp.75-86
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• 2017

The aerosol content dynamics in a virtual system were investigated. The outcome was extended to monitor the mean concentration diffusion of aerosols in a predefined macro and micro scale. The data set used were wind data set from the automatic weather station; satellite data set from Total Ozone Mapping Spectrometer aerosol index and multi-angle imaging spectroradiometer; ground data set from Aerosol robotic network. The maximum speed of the macro scale (West Africa) was less than 4.4 m/s. This low speed enables the pollutants to acquire maximum range of about 15 km. The heterogeneous nature of aerosols layer in the West African atmosphere creates strange transport pattern caused by multiple refractivity. It is believed that the multiple refractive concepts inhibit aerosol optical depth data retrieval. It was also discovered that the build-up of the purported strange transport pattern with time has enormous potential to influence higher degrees of climatic change in the long term. Even when the African Easterly Jet drives the aerosols layer at about 10 m/s, the interacting layers of aerosols are compelled to mitigate its speed to about 4.2 m/s (macro scale level) and boost its speed to 30 m/s on the micro scale level. Mean concentration diffusion of aerosols was higher in the micro scale than the macro scale level. The minimum aerosol content dynamics for non-decaying, logarithmic decay and exponential decay particulates dispersion is given as 4, 1.4 and 0 respectively.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.38.2-38.2
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• 2017

We present the observational study toward the multiple outflows around $LkH{\alpha}$ 234 star formation region. The high-resolution, near-IR spectral mapping using the Immersion Grating Infrared Spectrograph (IGRINS) allowed us to distinguish at least four separate outflows with the molecular hydrogen ($H_2$) and forbidden iron ([Fe II]) emission lines. The outflow associated with the radio continuum source VLA 3B is detected in both H2 and [Fe II] emission, while the outflows driven by MM 1, VLA 2 sources were only detected in $H_2$, indicating the different physical conditions of outflows. We confirm the axis of VLA 3B jet, the position angle of ${\sim}240^{\circ}$. We firstly identified the redshifted, near-IR H2 outflow associated with VLA 2, which is coincident with the previous detections of $H_2O$ masers. From the $H_2$ line ratios, we interpret the gas properties of the shock excited blue- and redshifted components, and UV excited surrounding photodissociation region. We also discuss the origin of the high-velocity (|VLSR| > $150km\;s^{-1}$) $H_2$ emission.