• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.1983-1993
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• 2017

Salmonella enterica subsp. enterica serovar Typhimurium, one of the most common foodborne pathogens, is transmitted mainly through contaminated food derived from infected animals. In this study, S. Typhimurium ST1120, an isolate from pig feces in Korea, was subjected to whole-genome analysis to understand its genomic features associated with virulence. The genome of ST1120 was found to have a circular chromosome of 4,855,001 bp (GC content 52.2%) and a plasmid of 6,863 bp (GC content 46.0%). This chromosome was predicted to have 4,558 open reading frames (ORFs), 17 pseudogenes, 22 rRNA genes, and 86 tRNA genes. Its plasmid was predicted to have three ORFs. Comparative genome analysis revealed that ST1120 was phylogenetically close to S. Typhimurium U288, a critical isolate in piggery farms and food chains in Europe. In silico functional analysis predicted that the ST1120 genome harbored multiple genes associated with virulence and stress resistance, including Salmonella pathogenicity islands (SPIs containing SPI-1 to SPI-5, SPI-13, and SPI-14), C63PI locus, ST104 prophage locus, and various antibiotic resistance genes. In accordance with these analysis results, ST1120 showed competence in invasion and survival abilities when it was added to host cells. It also exhibited robust resistance against antibiotics in comparison with other S. Typhimurium strains. This is the first report of the complete genome sequence of S. Typhimurium isolated from swine in Korea. Comparative genome analysis between ST1120 and other Salmonella strains would provide fruitful information toward understanding Salmonella host specificity and developing control measures against S. Typhimurium infection.

• The Plant Pathology Journal
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• 제34권2호
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• pp.150-156
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• 2018

The genome sequences of two novel monopartite RNA viruses were identified in a common eelgrass (Zostera marina) transcriptome dataset. Sequence comparison and phylogenetic analyses revealed that these two novel viruses belong to the genus Amalgavirus in the family Amalgaviridae. They were named Zostera marina amalgavirus 1 (ZmAV1) and Zostera marina amalgavirus 2 (ZmAV2). Genomes of both ZmAV1 and ZmAV2 contain two overlapping open reading frames (ORFs). ORF1 encodes a putative replication factory matrix-like protein, while ORF2 encodes a RNA-dependent RNA polymerase (RdRp) domain. The fusion protein (ORF1+2) of ORF1 and ORF2, which mediates RNA replication, was produced using the +1 programmed ribosomal frameshifting (PRF) mechanism. The +1 PRF motif sequence, UUU_CGN, which is highly conserved among known amalgaviruses, was also found in ZmAV1 and ZmAV2. Multiple sequence alignment of the ORF1+2 fusion proteins from 24 amalgaviruses revealed that +1 PRF occurred only at three different positions within the 13-amino acid-long segment, which was surrounded by highly conserved regions on both sides. This suggested that the +1 PRF may be constrained by the structure of fusion proteins. Genome sequences of ZmAV1 and ZmAV2, which are the first viruses to be identified in common eelgrass, will serve as useful resources for studying evolution and diversity of amalgaviruses.

• Journal of Korean Society of Steel Construction
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• 제21권3호
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• pp.321-330
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• 2009

This paper presents numerical analysis results for the lateral-torsional buckling (LTB) strength of steel I-girder bridges. Current Korean and AASHTO design specifications for LTB consider the buckling strength of a single girder with both its ends constrained. The I-girder bridges are composed of more than one girder, and the girders are interconnected with intermediate cross-beams or cross-frames. Therefore, it should be required to evaluate the effects of cross-beam stiffness and the interactionof girders on LTB strength. It is also necessary to consider the effects of transverse web stiffeners on LTB strength. By considering these parameters, a series of four-girder systemswere numerically modeled using 3D shell elements to estimate the LTB strength while considering initial imperfections and residual stresses.

• Korean Journal of Head & Neck Oncology
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• 제25권1호
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• pp.28-32
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• 2009

Background : The most frequently reported risk factors for head and neck suamous cell carcinoma are smoking and alcohol. But in a recent overview, human papilloma virus(HPV) infection was revealed the important carcinogenic factor in oropharyngeal cancer. We aimed to clarify whether HPV directly effects on the oncogenesis and biologic behavior of hean and neck squamous cell carcinoma by comparison with infection prevalence, and physical status of virus. Material and Method : We used HPV genotyping DNA chip(Biocore, Korea, Seoul) arrayed by multiple oligonucleotide probes of L1 sequence of 26 types of HPV and HPV genotypes are identified by fluorescence scanner. The copy numbers of HPV E2 and E6 open reading frames(ORF) were assessed using a TaqMan-based 5'-exonuclease quantitative real-time PCR assay. The ratio of E2 to E6 copy numbers was calculated to determine the physical status of HPV-16 viral gene. Results : We observed a significant difference in HPV prevalence between tonsillar cancer group and control group(73.1% vs. 11.6%), and most of the HPVs were type 16(87.2%) and integrated(94.1%) state. In terms of oral tongue cancer, we demonstrate that 30.5% has integrated HPV-16 in cancer tissue. But Glottic cancer only 1% is related to HPV-16 integration. Conclusion : This study revealed significant relationship of HPV prevalence with oropharyngeal and oral tongue squamous cell carcinoma. Most of HPV were 16 type and integrated or mixed, HPV-16 integration could be directly related to the carcinogenesis.

• Journal of KIISE:Computing Practices and Letters
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• 제8권6호
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• pp.647-656
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• 2002

Synchronization is the process of making replicated data on multiple devices be consistent, i.e., identical with each other. In Dec. 2000, major handheld computer manufacturers including Nokia, Ericsson, IBM published the SyncML protocol specification. In this paper, we describe the CNU SyncML server for PIMS(Personal Information Management System) service that we developed based on the SyncML specification. The server architecture and capabilities of the frames are presented along with the procedure of message processing by each frame. We put the CNU SyncML server to the conformance tests and interoperability tests to confirm its functionality. The session managing mechanism of the CNU SyncML server showed better performance than our previous implementations.

• The Journal of Korean Institute of Communications and Information Sciences
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• 제40권7호
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• pp.1387-1394
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• 2015

Along with the development of network transmission technology, the IPTV market is growing in fast pace. Additionally the UHD resolution broadcasting system along with user experience (UX) that provides better service to user has attracted attention recently since there are not enough research has been done with differentiated the UX that can enhance the UX yet. Therefore we proposed a low complexity syntax level image stitching implementation technique that run with multi-view services, which makes possibility to view multiple channel or video contents on the screen at the same time. Simulation results have demonstrated the liability and effectiveness of the proposed algorithm by showing that capability of generating more than 80 frames per second by stitching four Full-HD size videos into UHD frame.

• The Journal of Korean Institute of Communications and Information Sciences
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• 제41권3호
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• pp.317-327
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• 2016

The extraction of significant feature points from a video is directly associated with the suggested method's function. In particular, the occlusion regions in trees or people, or feature points extracted from the background and not from objects such as the sky or mountains are insignificant and can become the cause of undermined matching or recognition function. This paper classifies the feature points required for building recognition by using multi-frames in order to improve the recognition function(algorithm). First, through SIFT(scale invariant feature transform), the primary feature points are extracted and the mismatching feature points are removed. To categorize the feature points in occlusion regions, RANSAC(random sample consensus) is applied. Since the classified feature points were acquired through the matching method, for one feature point there are multiple descriptors and therefore a process that compiles all of them is also suggested. Experiments have verified that the suggested method is competent in its algorithm.

• Journal of the Korea Institute of Information and Communication Engineering
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• 제18권7호
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• pp.1663-1670
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• 2014

Recently, lots of research works have been actively focused on the DVC (Distributed Video Coding) techniques which provide a theoretical basis for the implementation of light video encoder. However, most of these studies have showed poorer performances than the conventional standard video coding schemes such as MPEG-1/2, MPEG-4, H.264 etc. In order to overcome the performance limits of the conventional approaches, several channel-divided distributed video coding schemes have been designed in such a way that some information are obtained while generating side information at decoder side and then these are provided to the encoder side, resulting in channel-divided video coding scheme. In this paper, the interpolation scheme by weighted sum of multiple motion-compensated interpolation frames is introduced and a new channel-divided DVC scheme is designed to effectively describe noisy channels based on the motion vector and its matching characteristics. Through several simulations, it is shown that the proposed method performs better than the conventional methods at low bit-rate and keeps the reconstructed visual quality constantly.

• Journal of the Institute of Electronics Engineers of Korea CI
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• 제40권1호
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• pp.30-38
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• 2003

This paper presents an efficient region-based motion segmentation method for segmenting of moving objects in a traffic scene with a focus on a Video Monitoring System (VMS). The presented method consists of two phases: motion detection and motion segmentation. Using the adaptive thresholding technique, the differences between two consecutive frames are analyzed to detect the movements of objects in a scene. To segment the detected regions into meaningful objects which have the similar intensity and motion information, the regions are initially segmented using a k-means clustering algorithm and then, the neighboring regions with the similar motion information are merged. Since we deal with not the whole image, but the detected regions in the segmentation phase, the computational cost is reduced dramatically. Experimental results demonstrate robustness in the occlusions among multiple moving objects and the change in environmental conditions as well.

• Journal of the Earthquake Engineering Society of Korea
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• 제10권3호
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• pp.65-75
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• 2006

Nonlinear pushover analysis is used to evaluate the earthquake response of building structures. To accurately predict the inelastic response of a structure, the prescribed story load profile should be able to describe the earthquake force profile which actually occurs during the time-history response of the structure. In the present study, a new modal combination method was developed to predict the earthquake load profiles of building structures. In the proposed method, multiple story load profiles are predicted by combining the modal spectrum responses multiplied by the modal combination factors. Parametric studies were performed far moment-resisting frames and walls. Based on the results. the modal combination factors were determined according to the hierarchy of each mode affecting the dynamic responses of structures. The proposed modal combination method was applied to prototype buildings with and without vertical irregularity. The results showed that the proposed method predicts the actual story load profiles which occur during the time-history responses of the structures.