• Mass Spectrometry Letters
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• v.2 no.4
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• pp.92-95
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• 2011

Pyrophosphates are the key intermediates in the biosynthesis of isoprenoids, and their concentrations could reveal the benefits of statins in cardiovascular diseases. Quantitative analysis of five pyrophosphates, including isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP), was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in negative ionization mode. After dilution with methanol, samples were separated on a 3 ${\mu}m$ particle multi-modal $C_{18}$ column ($50{\times}2$ mm) and quantified within 10 min. The gradient elution consists of 10 mM ammonium bicarbonate and 0.5% triethylamine (TEA) in water and 0.1% TEA in 80% acetonitrile was used at the flow rate of 0.4 mL/min. Overall recoveries were 51.4-106.6%, while the limit of quantification was 0.05 ${\mu}g$/mL for GPP and FPP and 0.1 ${\mu}g$/mL for IPP, DMAPP, and GGPP. The precision (% CV) and accuracy (% bias) of the assay were 1.9-12.3% and 89.6-111.8%, respectively, in 0.05-10 ${\mu}g$/mL calibration ranges ($R^2$ > 0.993). The devised LC-MS/MS technique with the multi-modal $C_{18}$ column can be used to estimate the biological activity of pyrophosphates in plasma and may be applicable to cardiovascular events with cholesterol metabolism as well as the drug efficacy of statins.

• Structural Engineering and Mechanics
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• v.25 no.1
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• pp.53-73
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• 2007

In this study, an effective load increment method for multi modal adaptive non-linear static (pushover) analysis (NSA) for building type structures is presented. In the method, lumped plastisicity approach is adopted and geometrical non-linearties (second-order effects) are included. Non-linear yield conditions of column elements and geometrical non-linearity effects between successive plastic sections are linearized. Thus, load increment needed for formation of plastic sections can be determined directly (without applying iteration or step-by-step techniques) by using linearized yield conditions. After formation of each plastic section, the higher mode effects are considered by utilizing the essentials of traditional response spectrum analysis at linearized regions between plastic sections. Changing dynamic properties due to plastification in the system are used on the calculation of modal lateral loads. Thus, the effects of stiffness changes and local mechanism at the system on lateral load distribution are included. By using the proposed method, solution can be obtained effectively for multi-mode whereby the properties change due to plastifications in the system. In the study, a new procedure for determination of modal lateral loads is also proposed. In order to evaluate the proposed method, a 20 story RC frame building is analyzed and compared with Non-linear Dynamic Analysis (NDA) results and FEMA 356 Non-linear Static Analysis (NSA) procedures using fixed loads distributions (first mode, SRSS and uniform distribution) in terms of different parameters. Second-order effects on response quantities and periods are also investigated. When the NDA results are taken as reference, it is seen that proposed method yield generally better results than all FEMA 356 procedures for all investigated response quantities.

• Journal of Korean Society of Steel Construction
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• v.26 no.6
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• pp.523-535
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• 2014

According to the capacity design concept underlying current steel seimsic provisions, the braces in concentrically braced frames should dissipate seismic energy through cyclic tension yielding and compression buckling. On the other hand, the beams and the columns in the braced bay should remain elastic for gravity load actions and additional column axial forces resulting from the brace buckling and yielding. However, due to the difficulty in accumulating the yielding and buckling-induced column forces from different stories, empirical and often conservative approaches have been used in design practice. Recently a totally different approach was proposed by Cho, Lee, and Kim (2011) for the prediction of column axial forces in inverted V-braced frames by explicitly considering brace buckling. The idea proposed in their study is extended to X-braced seismic frames which have structural member configurations and load transfer mechanism different from those of inverted V-braced frames. Especially, a more efficient rule is proposed in combining multi-mode effects on the column axial forces by using the modal-mass based weighting factor. The four methods proposed in this study are evaluated based on extensive inelastic dynamic analysis results.

• Earthquakes and Structures
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• v.19 no.5
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• pp.331-345
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• 2020

The multi-story timber structure with high platform base is one of the important architectural types in the traditional Chinese buildings. To study the dynamic characteristics and seismic responses on this kind of traditional structure, the 3-D finite element models of Xi'an drum tower which included the high platform base, upper timber structure and whole structure was established considering the structural form and material performance parameters of the structure in this study. By the modal analysis, the main frequencies and mode shapes of this kind of traditional building were obtained and investigated. The three kinds of earthquake excitations included El-Centro wave, Taft wave and Lanzhou wave were separately imposed on the upper timber structure model and the overall structure model, and the seismic responses on the tops of columns were analyzed. The results of time history analysis show that the seismic response of the upper timber structure is obviously amplified by high platform base. After considering the effect of high platform base, the mean value on the lateral displacement increments of the top column in the overall structure is more than 20.478% and the increase of dynamic coefficients was all above 0.818 under the above three different earthquake excitations. Obviously, it shows that the existence of high platform base has a negative influence on the seismic responses of upper timber structure. And the high platform base will directly affect the safety of the upper timber structure. Therefore, the influence of high platform base on the dynamic response of its upper timber structure cannot be neglected.

• Earthquakes and Structures
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• v.10 no.1
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• pp.1-23
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• 2016

Steel plate shear walls (SPSWs) have been shown to be efficient lateral force-resisting systems, which are increasingly used in new and retrofit construction. These structural systems are designed with either stiffened and stocky or unstiffened and slender web plates based on disparate structural and economical considerations. Based on some limited reported studies, on the other hand, employment of low yield point (LYP) steel infill plates with extremely low yield strength, and high ductility as well as elongation properties is found to facilitate the design and improve the structural behavior and seismic performance of the SPSW systems. On this basis, this paper reports system-level investigations on the seismic response assessment of multi-story SPSW frames under the action of earthquake ground motions. The effectiveness of the strip model in representing the behaviors of SPSWs with different buckling and yielding properties is primarily verified. Subsequently, the structural and seismic performances of several code-designed and retrofitted SPSW frames with conventional and LYP steel infill plates are investigated through detailed modal and nonlinear time-history analyses. Evaluation of various seismic response parameters including drift, acceleration, base shear and moment, column axial load, and web-plate ductility demands, demonstrates the capabilities of SPSW systems in improving the seismic performance of structures and reveals various advantages of use of LYP steel material in seismic design and retrofit of SPSW systems, in particular, application of LYP steel infill plates of double thickness in seismic retrofit of conventional steel and code-designed SPSW frames.