• 제목/요약/키워드: Multi-locus DNA dataset

검색결과 4건 처리시간 0.016초

Identification and Characterization of Pseudocercospora cornicola Causing Leaf Spots on Cornus officinalis

  • In-Young Choi;Ho-Jong Ju;Lamiya Abasova;Joon-Ho Choi;Hyeon-Dong Shin
    • 한국균학회지
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    • 제50권2호
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    • pp.131-136
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    • 2022
  • Cornus officinalis plants that grow in several locations in Korea have been found to be infected with leaf spot disease. Symptoms include necrotic lesions, which are angular, irregularly shaped, vein-limited, and dark brown, on both sides of the leaves. The causal agent of the disease was identified to be Pseudocercospora cornicola based on the morphological characteristics of the fungus and molecular phylogenetic analysis of the obtained multi-locus DNA sequence data. This is the first report investigating P. cornicola found on C. officinalis in Korea.

Morphology and Molecular Phylogeny of Pseudocercospora fatouae Associated with Hypophyllous Leaf Mold on Fatoua villosa in Korea

  • Kui-Jae Lee;In-Young Choi;Lamiya Abasova;Joon-Ho Choi;Jung-Hee Park;Hyeon-Dong Shin
    • 한국균학회지
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    • 제51권2호
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    • pp.147-153
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    • 2023
  • Dark brown leaf molds growing in a vein-limited pattern have been continuously observed on the lower leaf surface of Fatoua villosa in Korea. Fifteen samples deposited in the Korea University herbarium were examined for fungal morphological characterization. Two monoconidial isolates were obtained in 2022, and molecular analysis was performed based on a combined multigene dataset of the internal transcribed spacer (ITS) region and partial actin (actA), translation elongation factor 1-alpha (tef1), and DNA-directed RNA polymerase II second-largest subunit (rpb2). Based on morphological features and molecular phylogenetic analysis, the fungus Pseudocercospora fatouae was identified. To the best of our knowledge, it is the first study reporting the presence of P. fatouae on F. villosa in Korea.

New Finding of Golovinomyces salviae Powdery Mildew on Glechoma longituba (Lamiaceae), Besides Its Original Host Salvia spp.

  • In-Young Choi;Lamiya Abasova;Joon-Ho Choi;Young-Joon Choi;Hyeon-Dong Shin
    • 한국균학회지
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    • 제51권3호
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    • pp.239-243
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    • 2023
  • The Golovinomyces biocellatus complex is known to consist of powdery mildew from the Golovinomyces genus, associated with host plants from the Lamiaceae family. Recent molecular phylogenetic analyses have resolved the taxonomic composition of this complex, and Golovinomyces biocellatus sensu stricto is considered to be a pathogen of Glechoma species, globally. However, this paper presents a new finding of Golovinomyces salviae on Glechoma longituba, besides its original host species of Salvia. This information was inferred by molecular phylogenetic analyses from the multi-locus nucleotide sequence dataset of intergeneric spacer (IGS), internal transcribed spacer (ITS), large subunit (LSU) of rDNA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Further, the asexual morphology of this fungus is described and illustrated.

First Report of Xenoroussoella triseptata Isolated from Soil in Korea

  • Jung-Joo Ryu;Seung-Yeol Lee;In-Kyu Kang;Leonid N. Ten;Hee-Young Jung
    • 한국균학회지
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    • 제50권3호
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    • pp.195-204
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    • 2022
  • A fungal strain, designated KNUF-20-NI009, was isolated from soil collected from Gunsan-si, Jeollabuk-do, Korea. The isolate showed cultural features typical of the genus Xenoroussoella. Colonies cultivated on malt extract agar were olivaceous-brown to pale olivaceous-white at the margins, with undersides of dark olivaceous to olivaceous-brown and a white margin. The conidia, with a size range of 2.7-5.1×1.6-3.3 ㎛ ($\bar{x}=3.6\times2.6{\mu}m$, n=50), were globoid to ellipsoid in shape, hyaline when immature, becoming light brown to golden-brown when mature, and characterized by 1 or 2 guttules. Multi-locus sequence analysis based on a combined dataset of internal transcribed spacer regions (ITS), large subunit rDNA (LSU), small subunit rDNA (SSU), translation elongation factor 1-alpha (TEF1α), and RNA polymerase II largest subunit (RPB2) sequences revealed KNUF-20-NI009 to be a strain of Xenoroussoella triseptata. This is the first report of this species in Korea.