• Journal of Veterinary Science
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• 제25권3호
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• pp.43.1-43.13
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• 2024

Importance: Haemaphysalis longicornis is an obligate blood-sucking ectoparasite that has gained attention due its role of transmitting medically and veterinary significant pathogens and it is the most common tick species in Republic of Korea. The preferred strategy for controlling ticks is a multi-antigenic vaccination. Testing the efficiency of a combination antigen is a promising method for creating a tick vaccine. Objective: The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of H. longicornis by gene silencing. Methods: In this study, we used RNA interference to silence salivary enolase and subolesin in H. longicornis. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit's ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown. Results: Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (p < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction. Conclusions and Relevance: Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.

• 한국기계기술학회지
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• 제20권6호
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• pp.763-769
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• 2018

In this research, in order to improve the heat retention of greenhouse, comparative analysis of the heat flux of the marketed multi-later insulating curtain was carried out. Experiments is conducted by fabricating a test apparatus for investigating the heat flux characteristics. The multi-later insulating curtain used for the experiment was compared using the P, N, S, U and T company, which are commercially available, and the heat flux due to temperature difference between the experimental apparatus and the outside was compared and analyzed. When the internal temperature of the experimental result is the maximum temperature $60^{\circ}C$, the heat flux of multi-later insulating curtain is T Co.($73.1W/m^2$) > S Co.($119.5W/m^2$) > U Co.($155W/m^2$) > N Co.($163.1W/m^2$) > P Co.($177.7W/m^2$). The heat flux means the quantity of heat passing through the unit time per unit area, and the higher the numerical value, the higher the quantity of heat passing through the multi-layer insulating curtain. This can be determined that high heat fluxes produce low heat resistance. Further, it has been found that the weight of the insulating curtain is largely unrelated to the heat insulating property, and the heat insulating curtain having a thickness containing a high internal air layer is excellent in the heat insulating property. In the future when manufacturing a heat insulating curtain, It is judged that it is desirable to manufacture a combination of heat insulating materials that contain a high internal air layer content and that can maintain the air layer even for long-term use while minimizing the volume.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1255-1261
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• 2012

Thirty two Holstein female calves (initial body weight = $40{\pm}3.0$ kg) were used to investigate the effects of probiotic and prebiotic on average daily gain (ADG), fecal E. coli count, white blood cell count, plasma IgG1 level and cell-mediated immune response to injection of phytohemagglutinin in suckling female calves. Calves were assigned randomly to one of the four treatments, including whole milk without additives (control), whole milk containing probiotic, whole milk containing prebiotic and whole milk containing probiotic and prebiotic (synbiotic). Average daily gain was greater in calves fed probiotic, prebiotic and synbiotic at weeks 6, 7 and 8 (p<0.05). E. coli count was significantly lower in calves fed probiotic, prebiotic and synbiotic on d 56 (p<0.05). There was no significant difference between treatments in blood samples and cell-mediated response. This study showed that addition of probiotic, prebiotic and combination of these additives to milk enhanced ADG and reduced fecal E. coli count in preruminant calves.

• 한국융합학회논문지
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• 제9권2호
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• pp.13-18
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• 2018

본 논문은 다층을 갖는 네트워크를 가장 효율적으로 학습하는 것은 결국 최적의 가중치 벡터의 집합을 찾아가는 과정이라는 간단한 접근 방법을 기본으로 하고 있다. 일반적인 학습 문제의 단점을 극복하기 위해 제안 모델에서는 EBP와 OVSSA의 특징들을 결합한 방법을 사용한다. 즉 EBP가 지역 최소치에 빠질 수 있는 성질을 보강하기 위해 OVSSA의 확률이론으로 빠져나갈 수 있도록 제안 방법은 각각 알고리즘의 장점만을 취하여 하나의 모델을 구성한다. 제안 알고리즘에서는 EBP에서 오류를 줄이기 위한 방법들을 에너지함수로 사용하고, 이 에너지를 OVSSA로 최소화 하는 방법을 사용하였다. 두 가지의 상이한 성질을 가지는 알고리즘이 합쳐질 수 있음을 간단한 실험 결과를 통해 확인한다.

• 제어로봇시스템학회논문지
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• 제15권2호
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• pp.176-183
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• 2009

This paper proposes command signal generating method for a wearable robot using the force as the input signal. The basic concept of this system pursues the combination of the natural and sophisticated intelligence of human with the powerful motion capability of the robot. We define a task for the command signal generation to operate with the human body simultaneously, paying attention to comfort and ease of wear. In this study, we suggest a basic exoskeleton experimental system to evaluate a HRI(Human Robot Interface), selecting interfaces of arm braces on both wrists and a weight harness on the torso to connect the robot and human. We develop the HRI to provide a command for the robot motion. It connects between the human and the robot with the multi-axis load-cell, and it measures the relative force between the human and the robot. The control system calculates the trajectory of end-effector using this force signal. In this paper, we verify the performance of proposed system through the motion of elbow E/F(Extension/Flexion), the shoulder E/F and the shoulder Ab/Ad (Abduction/Adduction).

• Coupled systems mechanics
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• 제11권3호
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• pp.267-284
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• 2022

Due to the high usage of ABS in industries, such as aerospace, auto, recreational devices, boat, submarines, etc., the purpose of this project was to find a way to weld this material, which gives advantages, such as affordable, high speed, and good connection quality. In this experimental project, the friction welding method was applied with parameters such as numerical control (NC) machine with two different speeds and three cross-sections, including a flat surface, cone, and step. After the end of the welding process, samples were then applied for both tensile and bending tests of materials, and the results showed that, with increasing the machining velocity Considering of samples, the friction of the surface increased and then caused to increase in the surface temperature. Considering mentioned contents, the melting temperature of composite materials increased. This can give a chance to have a better combination of Nanomaterial to base melted materials. Thus, the result showed that, with increasing the weight percentage (wt %) of Nanomaterials contents, and machining velocity, the mechanical behavior of welded area for all three types of samples were just increased. This enhancement is due to the better melting process on the welded area of different Nano contents; also, the results showed that the shape of the welding area could play a significant role, and by changing the shape, the results also changed drastically.A better shape for the welding process was dedicated to the step surface.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2009년 춘계학술대회논문집
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• pp.57-65
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• 2009

An efficient and high-fidelity design approach for wing-body shape optimization is presented. Depending on the size of design space and the number of design of variable, aerodynamic shape optimization process is carried out via different optimization strategies at each design stage. In the first stage, global optimization techniques are applied to planform design with a few geometric design variables. In the second stage, local optimization techniques are used for wing surface design with a lot of design variables to maintain a sufficient design space with a high DOF (Degree of Freedom) geometric change. For global optimization, Kriging method in conjunction with Genetic Algorithm (GA) is used. Asearching algorithm of EI (Expected Improvement) points is introduced to enhance the quality of global optimization for the wing-planform design. For local optimization, a discrete adjoint method is adopted. By the successive combination of global and local optimization techniques, drag minimization is performed for a multi-body aircraft configuration while maintaining the baseline lift and the wing weight at the same time. Through the design process, performances of the test models are remarkably improved in comparison with the single stage design approach. The performance of the proposed design framework including wing planform design variables can be efficiently evaluated by the drag decomposition method, which can examine the improvement of various drag components, such as induced drag, wave drag, viscous drag and profile drag.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 세계 도시지반공학 심포지엄
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• pp.815-826
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• 2009

The paper presents a case study addressing the design and construction aspects for DCM(Deep Cement Mixing) method employed as the foundation of a caisson type breakwater with heavy weight(10,700 ton/EA) and a high design wave height($H_{1/3}$=8.7m). The DCM was designed for the project(Ulsan New Port North Breakwater Phase 1) by optimizing the pattern of DCM columns with a combination of short and long columns (i.e., block type(upper 3m)+wall type(lower)) and considering overlapped section between columns as a critical section against shear force where the coefficient of effective width of treated column($\alpha$) was estimated with caution. It was shown that the value can be 0.9 under the condition with the overlapped width of 30cm. In addition to that, a field trial test was performed after improving conventional DCM equipment (e.g., mixing blades, cement paste supplying pipes, multi auger motor, etc.) to establish a standardized DCM construction cycle (withdrawal rate of mixing blades) which can provide the prescribed strength. The result of the field strength test for cored DCM specimens shows that the averaged strength is larger than the target strength and the distribution of the strength(with a defect rate of 7%) also satisfies with the quality control normal distribution curve which allows defect rate of 15.9%.

• 대한전자공학회논문지TC
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• 제43권3호
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• pp.1-9
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• 2006

미래형 이동통신 시스템에서 셀 경계와 같이 반송파댄간섭전력비 (Carrier-to-Interference Ratio; CIR)가 낮은 열악한 채널 환경에서 하향링크 실시간 트래픽의 전송 성능 개선을 위한 Beanforming 기반 MIMO-OFDMA (Multi Input Multi Output-Orthogonal Frequency Division Multiple Access) 시스템을 제안한다. 우선 기본적인 MIMO-OFDM 시스템의 성능 향상을 위해 송신단 MRT (Maximum Ratio Transmission) 및 수신단 MRC (Maximum Ratio Combining) 기법의 연동을 고려하고, 이에 적합하도록 공간 자원 그룹화 기반의 CSI (Channel State Information) 계산법을 이용한 M-GTA-SBA (Modified-Grouped Transmit Antenna-Simple Bit Allocation) 기법을 고려한다 또한 Beamforming 적용으로 인한 상향링크에서의 과도한 궤환 정보량의 감소를 위해 Beam Weight 양자화 기반의 QEGT (Quantized Equal Gain Transmission) 기법을 적용하며, 다중 사용자 환경에서의 효율적인 자원 할당을 위해 P-SRA (Proposed Simple Resource Allocation) 알고리즘을 제안한다. 모의실험 결과, 제안된 시스템은 상향링크의 궤환 정보량을 감안하더라도 H-ARQ IR (Hybrid-Automatic Repeat Request Incremental Redundancy)과 Pseudo-Orthogonal Space Time Block Code를 사용하는 전형적인 개방루프형 MIMO-OFDMA 시스템에 비해 낮은 CIR 영역에서 월등히 개선된 주파수 효율 성능을 보임을 확인하였다.

• 대한의생명과학회지
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• 제16권4호
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• pp.213-220
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• 2010

Although an adequete intake of calcium (Ca) is recommended for the treatment and prevention of osteoporosis, the intake of Ca should be restricted because of its low rate of intestinal absorption. The purpose of this experiment was to identity the effect of the combined administration of Aquamin F (AQF) (a calcium agent) and lactic acid bacteria (LAB) on osteoporosis. Thirty ovariectomized (OVX) rats and six control rats were assigned to the following six groups, with six animals per group: sham Ca-deficient diet (Ca-D), OVX, LAB, AQF, and LAB-AQF. During the experiment, the body weight was measured; and after the experiment was completed, the serum biochemical analysis, the alkaline phosphatase, calcium, and inorganic phosphorus leves were measured. The tissue of the femur was stained and then scanned via CT. The body weight of the OVX group increased more significantly than that of the control group. The results of the bone mineral content (BMC), Bone mineral density (BMD), serum biochemical analysis and histological test on the femur epiphysis showed no difference between the OVX group and the LAB group, whereas the results of the AQF group were more significant than those of the OVX group. In particular, the LAB+AQF group showed more significant increases in the aforementioned results than the AQF group. This experiment showed that the combined administration of AQF and LAB in ovariectomized rats more significantly increased bone density than did a single administration of either AQF or LAB.