• Title/Summary/Keyword: Multi Presence

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Assessment of Bacterial Contamination and Antibiotic Resistance Patterns in Ready-To-Eat Side Dishes in Seoul Area, 2007 (2007년 서울 지역에서 판매되고 있는 반찬류의 세균 오염과 항생제 내성실태)

  • Kim, Mi-Jin;Lee, Do-Kyung;Jang, Seok;Yang, Hwan-Jin;An, Hyang-Mi;Baek, Eun-Hye;Ha, Nam-Joo
    • YAKHAK HOEJI
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    • v.52 no.6
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    • pp.434-440
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    • 2008
  • The purpose of this research was to investigate the microbiological quality and antimicrobial susceptibilities of the ready-to-eat side dishes, which are were collected from the grocery stores, big markets, and department stores in Seoul throughout November, 2007. Of total 124 samples, presence of staphylococci and enteric bacilli was observed in 38 samples (31%) and 53 samples (43%), respectively. And 30 samples (24%) were shown to be of unsatisfactory quality for total bacterial count (>$10^5$). Antimicrobial susceptibilities of the staphylococci isolated from the side dishes were tested for six different antimicrobial agents, which are in widespread clinical use in Korea, as well as four new antimicrobials, daptomycin, linezolid, quinupristin/dalfopristin and tigecycline. As a result, the staphylococcal isolates were found to be resistant to oxacillin (${MIC}_{90}$, >128 ${\mu}g$/ml), teicoplanin (${MIC}_{90}$, >128 ${\mu}g$/ml), mupirocin (${MIC}_{90}$, >128 ${\mu}g$/ml), linezolid (${MIC}_{90}$, 128 ${\mu}g$/ ml) and quinupristin/dalfopristin (${MIC}_{90}$, 32 ${\mu}g$/ml). Especially, some of the staphylococcal isolates exhibited high level and multi-drug resistance. Moreover, these bacteria were also resistant to new antimicrobials, except tigecycline.

Toxigenic Mycobiota of Small Grain Cereals in Korea

  • Lee, Theresa
    • 한국균학회소식:학술대회논문집
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    • 2016.05a
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    • pp.33-33
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    • 2016
  • Mycotoxins are toxic secondary metabolites produced by fungi. They can be present in where agricultural-based commodities are contaminated with toxigenic fungi. These mycotoxins cause various toxicoses in human and livestock when consumed. Small grains including corn, barley, rice or wheat are frequently contaminated with mycotoxins due to infection mainly by toxigenic Fusarium species and/or under environment favorable to fungal growth. One of the most well-known Fusarium toxin groups in cereals is trichothecenes consisting of many toxic compounds. Deoxynivalenol (DON), nivalenol (NIV), T-2 toxin, and various derivatives belong to this group. Zearalenone and fumonisin (FB) are also frequently produced by many species of the same genus. In order to monitor Korean cereals for contamination with Fusarium and other mycotoxigenic fungal species as well, barley, corn, maize, rice grains, and soybean were collected from fields at harvest or during storage for several years. The fungal colonies outgrown from the grain samples were identified based on morphological and molecular characteristics. Trichothecene chemotypes of Fusarium species or presence of FB biosynthetic gene were determined using respective diagnostic PCR to predict possible toxin production. Heavy grain contamination with fungi was detected in barley, rice and wheat. Predominant fungal genus of barley and wheat was Alternaria (up to 90%) while that of rice was Fusarium (~40%). Epicoccum also appeared frequently in barley, rice and wheat. While frequency of Fusarium species in barley and wheat was less than 20%, the genus mainly consisted of Fusarium graminearum species complex (FGSC) which known to be head blight pathogen and mycotoxin producer. Fusarium composition of rice was more diverse as FGSC, Fusarium incarnatum-equiseti species complex (FIESC), and Fusarium fujikuroi species complex (FFSC) appeared all at considerable frequencies. Prevalent fungal species of corn was FFSC (~50%), followed by FGSC (<30%). Most of FFSC isolates of corn tested appeared to be FB producer. In corn, Fusarium graminearum and DON chemotype dominate within FGSC, which was different from other cereals. Soybeans were contaminated with fungi less than other crops and Cercospora, Cladosporium, Alternaria, Fusarium etc. were detected at low frequencies (up to 14%). Other toxigenic species such as Aspergillus and Penicillium were irregularly detected at very low frequencies. Multi-year survey of small grains revealed dominant fungal species of Korea (barley, rice and wheat) is Fusarium asiaticum having NIV chemotype.

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Preparation and Characteristics of MWNT/SnO2 Nano-Composite Anode by Homogeneous Precipitation Method (균일 침전법에 의한 MWNT/SnO2 나노복합음극재의 제조)

  • Han, Won-Kyu;Choa, Yong-Ho;Oh, Sung-Tag;Cho, Jin-Ki;Kang, Sung-Goon
    • Korean Journal of Materials Research
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    • v.18 no.4
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    • pp.187-192
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    • 2008
  • Multi-walled carbon nanotube (MWNT)/$SnO_2$ nano-composite (MSC) for the anode electrode of a Li-ion battery was prepared using a homogeneous precipitation method with $SnCl_2$ precursors in the presence of MWNT. XRD results indicate that when annealed in Ar at $400^{\circ}C$, $Sn_6O_4(OH)_4$ was fully converted to $SnO_2$ phases. TEM observations showed that most of the $SnO_2$ nanoparticles were deposited directly on the outside surface of the MWNT. The electrochemical performance of the MSC electrode showed higher specific capacities than a MWNT and better cycleability than a nano-$SnO_2$ electrode. The electrochemical performance of the MSC electrode improved because the MWNT in the MSC electrode absorbed the mechanical stress induced from a volume change during alloying and de-alloying reactions with lithium, leading to an increase in the electrical conductivity of the composite material.

Thermal and Electrical Properties of PS/MWCNT Composite Prepared by Solution Mixing: Effect of Surface Modification of MWCNT (Solution Mixing법에 의한 PS/MWCNT 복합재료의 열 및 전기전도 특성: MWCNT 표면 개질의 영향)

  • Park, Eun-Ju;Lee, Jeong-Woo;Jung, Dong-Soo;Shim, Sang-Eun
    • Elastomers and Composites
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    • v.45 no.1
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    • pp.17-24
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    • 2010
  • Herein, the effect of the dispersion uniformity of the multi-wall carbon nanotube (MWCNT) on the thermal and electrical conductivity of polystyrene (PS)/MWCNT composite was investigated. The PS/MWCNT composites were prepared by solution mixing from dispersions of various MWCNTs in PS/tetrahydrofuran (THF) solution. Three types of MWCNTs were used; pristine MWCNT, hydroxyl functionalized MWCNT, which was functionalized with $KMnO_4$ in the presence of a phase transfer catalyst at room temperature, and pristine MWCNT with BYK-9077 as a dispersant. It was found that the stable dispersion state of MWCNT in PS/THF solutions significantly improved the thermal and electrical conductivity of the ultimate composites. It is noted that the thermal and electrical conductivity of PS/3 wt% pristine MWCNT composite with BYK-9077 were about 9.4 and 30~50% higher than those of PS/3 wt% pristine MWCNT composite, respectively.

Enhancement of Pyruvate Production by Torulopsis glabrata : through Supplement of Oxaloacetate as Carbon Source

  • Liu Li-Ming;Du Guo-Cheng;Li Vin;Li Hua-Zhong;Chen Jian
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.2
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    • pp.136-141
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    • 2005
  • The capability of utilizing a TCA cycle intermediates as the sole carbon source by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019 was demonstrated with plate count method. It is indicated that T. glabrata could grew on a medium with one of the TCA cycle intermediates as the sole carbon source, but more colonies were observed when glucose, acetate and one of the TCA cycle intermediates coexisted in the medium. Among the intermediates of the TCA cycle examined in this study, cell growth was improved by supplementing oxaloacetate. Further investigation showed that the presence of acetate was necessary when oxaloacetate was supplemented. By supplementing with 10 g/L of oxaloacetate in pyruvate batch fermentation, dry cell weight increased from 11.8 g/L to 13.6 g/L, and pyruvate productivity was enhanced from $0.96\;gL^{-1}h^{-1}\;to\;1.19 gL^{-1}h^{-1}$ after cultivation of 56 h. The yield of pyruvate to glucose was also improved from 0.63 g/g to 0.66 g/g. These results indicate that under vitamins limitation, the productivity and yield of pyruvate could be enhanced via an increase of cell growth by the supplementation of oxaloacetate.

Classification Prediction Error Estimation System of Microarray for a Comparison of Resampling Methods Based on Multi-Layer Perceptron (다층퍼셉트론 기반 리 샘플링 방법 비교를 위한 마이크로어레이 분류 예측 에러 추정 시스템)

  • Park, Su-Young;Jeong, Chai-Yeoung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.2
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    • pp.534-539
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    • 2010
  • In genomic studies, thousands of features are collected on relatively few samples. One of the goals of these studies is to build classifiers to predict the outcome of future observations. There are three inherent steps to build classifiers: a significant gene selection, model selection and prediction assessment. In the paper, with a focus on prediction assessment, we normalize microarray data with quantile-normalization methods that adjust quartile of all slide equally and then design a system comparing several methods to estimate 'true' prediction error of a prediction model in the presence of feature selection and compare and analyze a prediction error of them. LOOCV generally performs very well with small MSE and bias, the split sample method and 2-fold CV perform with small sample size very pooly. For computationally burdensome analyses, 10-fold CV may be preferable to LOOCV.

연안 항행안전 위험시설 정보 취득 및 활용 기법

  • Yang, Chan-Su
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2009.10a
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    • pp.73-74
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    • 2009
  • This study attempts to establish a system extracting and monitoring cultural grounds of seaweeds (lavers, brown seaweeds and seaweed fulvescens) and abalone on the basis of both KOMPSAT-2 and Terrasar-X data. The study areas are located in the northwest and southwest coast of South Korea, famous for coastal cultural grounds. The northwest site is in a high tidal range area (on the average, 6.1 m in Asan Bay) and has laver cultural grounds for the most. An semi-automatic detection system of laver facilities is described and assessed for spaceborne optic images. On the other hand, the southwest cost is most famous for seaweeds. Aquaculture facilities, which cover extensive portions of this area, can be subdivided into three major groups: brown seaweeds, capsosiphon fulvescens and abalone farms. The study is based on interpretation of optic and SAR satellite data and a detailed image analysis procedure is described here. On May 25 and June 2, 2008 the TerraSAR-X radar satellite took some images of the area. SAR data are unique for mapping those farms. In case of abalone farms, the backscatters from surrounding dykes allows for recognition and separation of abalone ponds from all other water-covered surfaces. But identification of seaweeds such as laver, brown seaweeds and seaweed fulvescens depends on the dampening effect due to the presence of the facilities and is a complex task because objects that resemble seaweeds frequently occur, particularly in low wind or tidal conditions. Lastly, fusion of SAR and optic spatial images is tested to enhance the detection of aquaculture facilities by using the panchromatic image with spatial resolution 1 meter and the corresponding multi-spectral, with spatial resolution 4 meters and 4 spectrum bands, from KOMPSAT-2. The mapping accuracy achieved for farms will be estimated and discussed after field verification of preliminary results.

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Antibacterial Mode of Action of Cinnamomum verum Bark Essential Oil, Alone and in Combination with Piperacillin, Against a Multi-Drug-Resistant Escherichia coli Strain

  • Yap, Polly Soo Xi;Krishnan, Thiba;Chan, Kok-Gan;Lim, Swee Hua Erin
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1299-1306
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    • 2015
  • This study aims to investigate the mechanism of action of the cinnamon bark essential oil (CB), when used singly and also in combination with piperacillin, for its antimicrobial and synergistic activity against beta-lactamase TEM-1 plasmid-conferred Escherichia coli J53 R1. Viable count of bacteria for this combination of essential oil and antibiotic showed a complete killing profile at 20 h and further confirmed its synergistic effect by reducing the bacteria cell numbers. Analysis on the stability of treated cultures for cell membrane permeability by CB when tested against sodium dodecyl sulfate revealed that the bacterial cell membrane was disrupted by the essential oil. Scanning electron microscopy observation and bacterial surface charge measurement also revealed that CB causes irreversible membrane damage and reduces the bacterial surface charge. In addition, bioluminescence expression of Escherichia coli [pSB1075] and E. coli [pSB401] by CB showed reduction, indicating the possibility of the presence of quorum sensing (QS) inhibitors. Gas-chromatography and mass spectrometry of the essential oil of Cinnamomum verum showed that trans-cinnamaldehyde (72.81%), benzyl alcohol (12.5%), and eugenol (6.57%) were the major components in the essential oil. From this study, CB has the potential to reverse E. coli J53 R1 resistance to piperacillin through two pathways; modification in the permeability of the outer membrane or bacterial QS inhibition.

The Origin of the Southeastern United States Continental Margin: Is it Volcanic or Non-Volcanic? (미국남동부 대륙주변부의 기원 : 화산성 혹은 비화산성?)

  • Oh, Jinyong
    • Economic and Environmental Geology
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    • v.27 no.6
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    • pp.571-577
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    • 1994
  • It has been controversial whether the United States Atlantic margin, which developed during Mesozoic separation of Africa and North America, is a volcanic or non-volcanic rifted margin. To understand its nature, the basement images of multi-channel seismic profiles off the southeastern United States continental margin have been examined. One of prominent results is the presence of seaward-dipping reflector (SDR) wedges, the most diagnostic feature of volcanic rifted margins. Two sets of SDR wedges appear to exist here; one along the basement hinge zone ('the hinge SDR wedge') and another seaward of the East Coast magnetic anomaly ('the outer SDR wedge'). Seaward of the basement hinge zone, the lower crustal high-velocity body previously known as the 7.2 km/s layer and the underlying smooth Moho configuration are also observed. Based on the comparison of these basement images with the crustal structures of the well-known volcanic rifted margin, the southeastern United States Atlantic margin can now be characterized as a typical volcanic rifted margin.

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Residents' Satisfaction with Visual Appearance of the Housing Environment in Korean Multi-Family Housing (주거환경에 대한 거주자 시각만족의 경험적 모형)

  • 진양교
    • Journal of the Korean Institute of Landscape Architecture
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    • v.21 no.4
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    • pp.15-27
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    • 1994
  • 주거환경에서 거주자의 '시각만족(residents' satisfaction with visual appearance)은 더 큰 추상적 개념인 '주거만족'(residents' housing satisfaction)과의 밀접한 인과적 관련(Francescato et. al., 1979) 때문뿐만 아니라 인간 기본 욕구 중의 하나인 미적 욕구의 충족과 관련되어 있다는 점(Chin, 1991)에서도 중요시 되어야 할 이론적 개념이다. 본 연구에서는 거주자의 시각만족을 종속지표로 보고, 다양한 영향 가설변수들과의 인과 적 관련도를 경로분석(path analysis)을 통해 검증하고 있으며, 어떤 변수 가 어떤 이유(경로)로 또는 얼마만한 크기로 거주자의 시각만족에 영향을 주는 가가 중점적으로 토의된다. 한국의 6개 대단위 주거단지로부터 다단 계표집 방법과 설문면담 방법을 통해 646명의 응답이 수거되었다. 분석의 첫 단계로써, 다수의 설문문항을 본 연구의 가설 변수로 집약하기 위한 요 인분석(factor analysis)이 사용되었고, 요인 분석으로부터 추출된 변수들간 의 인과적 관련도를 검증하기 위해 최종적으로 경로분석이 사용되었다. 본 연구의 결과를 요약해 볼 때, 본 연구의 가설 변수 대부분이 최종 종속변 수인 거주자의 '시각만족'에 유의한 인과 영향을 보이는 것으로 나타났으 며, 구체적으로 '시각적 무질서'(visual disorder), '시각적 단조로움'(visual monotony), '단지 과밀감'(development crowding) 등이 부정적 영향을, '시 각적 매력'(visual attractiveness)과 '인근 자연녹지의 존재'(presence of nearby natural environment)등이 긍정적 영향을 미치는 것으로 파악되었 다. 그 외에도 '옥외공간이용 편리'(outdoor or recreation convenience)와 ' 이웃만족'(satisfaction with neighbors), 그리고 '주거환경 유형'(building type, building arrangement type)등도 유의한 인과적 관련을 보이므로써, 기존 문헌들이 제시하고 있는 것보다 훨씬 다양한 변수들이 다양한 경로를 통해 거주자 시각만족의 영향인자가 될 수 있는 가능성을 제시하고 있다. 가설 변수의 하나인 '길찾기의 난이 정도'(difficulty of way-finding)와 종 속변수간에 유의한 관련도가 나타나지 않은 이유로 길찾기 변수가 '시각만 족'보다는 거주자의 '안전만족'(safety)과 관련된 변수일 가능성도 아울러 지적되었다. 본 연구의 결과로부터, 주거 계획 및 설계분야 그리고 추후 관 련 연구 분야를 위한 여러 제안들이 제시되었다.

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