• 한국발생생물학회지:발생과생식
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• 제20권3호
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• pp.207-217
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• 2016

In this study, we investigated the characteristics of CCK-producing cells and mucus-secreting goblet cells with respect to stomach fish and stomachless fish of the Gobiidae in order to provide a basis for understanding the digestive physiology. Hairychin goby (Sagamia geneionema), which is stomachless fish, the numbers of mucus-secreting goblet cells is highest in the posterior intestine portion (P<0.05), while CCK-producing cells are scattered throughout the intestine. Gluttonous goby (Chasmichthys gulosus), which is stomach fish, mucus-secreting goblet cells are most abundant in the mid intestine portion (P<0.05), whereas CCK-producing cells are observed only in the anterior and mid intestine portion. Trident goby (Tridentiger obscurus) which is stomach fish, mucus-secreting goblet cells were most abundant in the mid intestine portion (P<0.05). CCK-producing cells are found in the anterior and mid intestine portion. Giurine goby, Rhinogobius giurinus which is also stomach fish, the largest number of mucus-secreting goblet cells showed in anterior intestine portion except for esophagus (P<0.05). CCK-producing cells are present only in the anterior and mid intestine portion. In S. geneionema, digestive action occurs in the posterior intestine portion to protect and functions to activate digestion. In contrast, in C. gulosus, T. obscurus and R. giurinus, their digestive action occurs in the anterior and mid intestine portion to protect and functions to activate digestion. Further studies of the modes of food ingestion by these fish, the contents of their digestive tracts, and the staining characteristics of the goblet cells need to be carried out.

• Fisheries and Aquatic Sciences
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• 제8권2호
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• pp.83-89
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• 2005

Morphology, histology, and histochemical characteristics of the digestive tract of the guirine goby (Rhinogobius giurinus: Gobiidae), collected from the coast of Jeju Island, Korea, were investigated. The digestive tract of R. giurinus, which is a brackish water species, has a short (relative length of gut=0.42), simple, and narrow gut. The gastric glands are well developed in the stomach, but pyloric caeca are absent. The mucosal folds are regularly branched, and the muscularis extern a is thickest in the esophagus, which also contained the most mucus-secreting goblet cells (P<0.05). In R. giurinus, digestive function occurs in the anterior portion of the digestive tract, where mucus protects the tract from the actions of digestive enzymes and functions to activate digestion.

• Applied Microscopy
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• 제6권1호
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• pp.9-20
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• 1976

성숙한 정상마우스 ( 체중 약 20gr)의 위장관 점막 내에 출현하는 점액함유 세포의 점액질의 화학적 조성을 구명하고자 PAS, AB pH 2.5-PAS, AB pH 1.0-PAS 염색법을 이용하여 중성점액질, 황화점액질 및 산성점액질의 염색반응의 유무 내지 강약으로서 비교 검토하였고, 또 전자현미경을 이용하여 이들 세포의 미세구조를 관찰하였다. 위체부 표면상피에 출현하는 점액함유 세포는 주성분이 중성점액질이고, 약간의 약산성 점액질도 존재하였다. 위소와에 있는 점액세포와 점액경세포는 중성점액질을 함유하고 있었다. 소장의 점막상피에 출현하는 배상세포에서는 약산성, 강산성, 황화점액질이 주성분이었고, 약간의 중성점액질을 함유하고 있었다. 한편, 장선의 장상세포는 비황화 약산성 점액질을 갖고 있다. 대장의 점막상피에 출현하는 배상세포는 표면상피에서는 소장과 대동소이 하였으나 은와부에서는 중성 및 약산성점액질이 주성분이었다. 위체부 표면상피에 있는 점액세포의 과랍은 전자밀도가 높은 것이 대부분이었고, 전자밀도가 낮은 것이 소수 출현하였다. 점액경세포의 점액과립은 전자밀도가 낮았으나, 그 속에 dense core 가 있는 것이 출현하였다. 대 소장 점막상피에 출현하는 배상세포는 모두 전자밀도가 낮은 과립이 존재하였다. 위장관의 점액세포들은 모두 부분분필방식을 취하고 있었다.

• Nutrition Research and Practice
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• 제11권6호
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• pp.461-469
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• 2017

BACKGROUND/OBSECTIVE: Airway inflammation by eosinophils, neutrophils and alveolar macrophages is a characteristic feature of asthma that leads to pathological subepithelial thickening and remodeling. Our previous study showed that oxidative stress in airways resulted in eosinophilia and epithelial apoptosis. The current study investigated whether glutathione-containing dry yeast extract (dry-YE) ameliorated eosinophilia, goblet cell hyperplasia and mucus overproduction. MATERIALS/METHOD: This study employed $2{\mu}g$/mL lipopolysaccharide (LPS)- or 20 ng/mL eotaxin-1-exposed human bronchial epithelial cells and ovalbumin (OVA)-challenged mice. Dry-YE employed in this study contained a significant amount of glutathione (140 mg in 100 g dry yeast). RESULTS: Human bronchial epithelial cell eotaxin-1 and mucin 5AC (MUC5AC) were markedly induced by the endotoxin LPS, which was dose-dependently attenuated by nontoxic dry-YE at 10-50 ${\mu}g$/mL. Moreover, dry-YE inhibited the MUC5AC induction enhanced by eotaxin-1, indicating that eotaxin-1-mediated eosinophilia may prompt the MUC5AC induction. Oral supplementation with 10-100 mg/kg dry-YE inhibited inflammatory cell accumulation in airway subepithelial regions with a reduction of lung tissue level of intracellular adhesion molecule-1. In addition, ${\geq}50$ mg/kg dry-YE diminished the lung tissue levels of eotaxin-1, eosinophil major basic protein and MUC5AC in OVA-exposed mice. Alcian blue/periodic acid schiff staining revealed that the dry-YE supplementation inhibited goblet cell hyperplasia and mucus overproduction in the trachea and bronchiolar airways of OVA-challenged mice. CONCLUSIONS: Oxidative stress may be involved in the induction of eotaxin-1 and MUC5AC by endotoxin episode and OVA challenge. Dry-YE effectively ameliorated oxidative stress-responsive epithelial eosinophilia and mucus-secreting goblet cell hyperplasia in cellular and murine models of asthma.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1995년도 춘계학술대회
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• pp.89-89
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• 1995

Surface epithelial cells isolated from hamster tracheas and grown on a thick collagen gel become a highly enriched population of mucus-secreting cells. Epithelial cells from tracheas of hamsters were collected using enzymatic procedures and cultured under various conditions. The medium used consisted of a 1:1 mixture of medium 199 and Dulbecco's modified Eagle's (DME) medium which was conditioned before use. Insulin, transferrin, hydrocortisone, epidermal growth factor, and extract from bovine hypothalamus were used as supplement. Due to relatively low basal rates of min secretion from in vitro cultures, cultures are generally radiolabeled using $^3$H-glucosamine as a metabolic precursor. The radiolabeled mucinsreleased are quantitated by precipitation with TCA/PTA. Using this cell culture system, we investigated mucin release of goblet cells by altering the media bathing the apical surface of hamster tracheal surface epithelial(HTSE) cells. Acidic media added sulfuric acid caused sigcificant increases in mucin relesse (155${\pm}$20% at pH 4 and 146${\pm}$16% at, pH 5). Ammonium hydroxide also increased mucin release at pH 9.0(156${\pm}$17%) and pH 10(295${\pm}$9%) respectively. This additional mucin release seems to be associated with cell membrane damage as indicated by release of cellular LDH. SP stimulates secretion of mucin in cultured HTSE cells(154${\pm}$16% at 1${\times}$10$\^$-6/M and 165${\pm}$25% at 1${\times}$10$\^$-5/M. PAF at 5${\times}$10$\^$-6/M and 5${\times}$10$\^$-5/M enhanced by HTSE cells in vitro 168${\pm}$34% and 259${\pm}$30% of mucin secretion, respectively. The increase in mucin release by PAF and SP was not secondary to cell damage or necrosis. SP and PAF may be in mediating mucous secretion induced by inflammation irritantion and infection.

• 한국수산과학회지
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• 제52권4호
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• pp.358-365
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• 2019

This study investigated the effects of dietary cactus Opuntia ficus-indica stem and fruit extract on the growth, flesh quality, lysozyme activity, and histological changes of growing Korean rockfish Sebastes schlegeli. Three replicates of fish (152 g/fish) were fed one of the following diets: containing 0 additions (control); 0.1, 0.5, or 1.0% cactus stem powder; or 1.0% fruit extract for 11 weeks. Growth performance did not differ significantly among treatments, including survival, final weight, feed efficiency, and daily feed intake. The experimental diets did not affect the proximate and fatty acid compositions, plasma biochemistry, or dorsal muscle texture of the fish. However, the plasma lysozyme activity of the fish fed the diet containing 0.1% cactus stem was significantly higher than that of the fish fed the control diet. These fish had variously sized lipid vacuoles in the liver tissue compared with the control. Distinct mucosal folds and mucus-secreting goblet cells developed in the fish fed the diet containing 1% cactus stem compared with the other dietary groups. These results suggest that feeding growing Korean rockfish cactus stem might increase the plasma lysozyme activity and induce histological changes in the gastrointestinal tract that might be related to digestion.