• The Korean Journal of Zoology
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• v.18 no.2
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• pp.51-60
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• 1975

The present study was performed histologically and histochemically to observe the cutaneous mucous glands in the frog, Rana nigromaculata during metamorphosis. The cutaneous thssues including dermal plicae in the dorsal portions of the frog tadpoles at each metamorphosis stage were fixed in 10% buffered formalin at$4^{\circ}C$, embedded in paraffin wax, sectioned 4 $\mu$m thickness and stained with periodic acid-Schiff(PAS) and alcian blue (AB) at both pH 2.5 and pH 1.0. The results of observation were as follows: 1. The developments of cutaneous mucous glands of the frog tadpole were begun with appearance of gland cell nest in the dermis at metamorphosis XV stage and significant numerical increases could be seen at metamorphosis XX, XXIII and XXIV stages. 2. This cutaneous mucous gland of the frog tadpole could be divided into two types; A-type glands showed strong positivities to both PAS and AB at pH 2.5 in the gland body cells and to PAS in the gland neck cells, and B-type glands at AB pH 2.5 in the gland body cells. 3. In the A-type mucous glands, the reactivities of the glandular epithelial cells to both PAS and AB stain could be first seen at the metamorphosis XIX stage of frog tadpole. The reactivities of the glandular epithelial cells to both PAS and AB pH 2.5 were gradually increased according to the process of metamorphosis after XX stage of metamorphosis. 4. The B-type mucous glands were first seen at the XX stage and the reactivity of the glandular epithelial cells to AB at pH 2.5 was gradually increased according to the process of metamorphosis after XX stage. 5. The A-type and the B-type mucous glands were in the ratio of 99 : 1, 7 : 3 and 5.5 : 4.5 for each of metamorphosis XX, XXI-XXII and XXIII-XXV stages. 6. The remarkable development of the cutaneous mucous glands of the frog tadpoles might be needed to maintain water and electrolyte balances according to the change of way from aquatic life to amphibious.

• The Korean Journal of Malacology
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• v.30 no.1
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• pp.51-56
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• 2014

Morphology and histochemistry of gill of the abalone, Haliotis discus hannai were described using light microscopy and scanning electron microscopy (SEM). The abalone has bipectinate gill of protobranch. The cilia and microvilli were well-developed on the free surface of the gill filaments. The epithelial layer of gill filament was simple and composed columnar epithelia and mucous cells. Result of AB-PAS (pH 2.5 and 1.0) and AF-AB (pH 2.5) reaction showed that mucous cells contained mainly acidic carboxylated mucosubstances.

• Journal of fish pathology
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• v.21 no.1
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• pp.67-79
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• 2008

This study was conducted to find out biological response of bivalves exposed to tributyltin chloride(TBTCl). The results of the study confirmed that TBTCl induce the reduction of oxygen consumption rateand histopathological feature in the gill structure of equilateral venus, Gomphina veneriformis. The experi-mental groups consisted of a control and 3 TBTCl exposure groups (0.4, 0.6 and 0.8 yg TBTCl L') and theexperimental period was 36 weeks. For histological analysis, gill tissues were fixed in Bouin's fluid andthen stained H-E stain, AB-PAS (PH 2.5) reaction and Masson's trichrome stain after having serially sec-tioned the tissue by paraffin method at thickness of 4-6 (an. The oxygen consumption rate was not signifi-cantly different between the control and exposure groups at 4 weeks, but in all exposure groups at 28 weeks,it was significantly different to the control. Gill of G. veneriformis had demibranch that attached two sheetsof lamellae and a lamella was composed of numerous filaments, numbering 25 on average. The frontal fila-ment zone had three types of cilia; frontal, latero-frontal and lateral depending on locations while the lateralcilia were the longest and largest in number. The mucous cells observed in filaments were more abundant in(542c) in AB-PAS (PH 2.5) reaction. Gill exposed to TBTCl was extended hemolymph sinus and increased hemocytes at 4 weeks, and then it showed increases of mucous cells and partially disappearance of frontalcilia. In the group of 0.8 yg TBTCl L' at 12 weeks, hypertrophy of frontal and latero-frontal epithelia wasobserved. Also it observed m decrease of mucous cell containing weekly acid mucosubstance and appearedpartially destruction muscle fiber bundle, In the groups of 0.4 and 0.6 ug TBTCl L' at 36 weeks, it appearedpartially modification of epithelia and in 0.8 us TBTCl L' group, observed filaments that come out chiti-nous rod from disappearance of frontal and latero-frontal epithelia.

• Korean Journal of Ichthyology
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• v.28 no.4
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• pp.223-228
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• 2016

The anatomy and histology of the olfactory organ in Oryzias sinensis was researched using a stereo microscope and light microscope. In the gross structure, the paired olfactory organs on the dorsal part of the head consist of two nostrils (a circular anterior nostril and a slit posterior nostril in a distance), a single olfactory chamber and a single accessory nasal sac. In the histological study, the epithelium of the olfactory chamber is classified into both sensory and non-sensory regions. The sensory epithelium consists of olfactory receptor neurons, supporting cells, basal cells and vesicles, and is islet in distributional pattern. The non-sensory epithelium is composed of stratified epithelial cells and two types of mucous cells (acidic and neutral cells). The epithelium of the accessory nasal sac has swollen stratified epithelial cells, mucous cells with a rich glycoprotein. Such an olfactory anatomy and histology of O. sinensis may reflect its habitat surrounding stagnant and polluted water.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.513-515
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• 2000

타액선을 구성하는 선세포의 종류는 달팽이의 종류에 따라 다양하게 나타났던 바, Walker(1970)는 병안목(Agriolimax reticiulatus)의 타액선을 광학현미경을 이용하여 2개의 성점액세포와 7개의 중성 및 장액성세포 그리고 1개의 순수한 장액세포 등 모두 10개의 세포를 관찰한 바 있고, Mclean(1970)은 Haliotis rufescens를 재료로 한 실험에서 타액선을 성하는 선세포는 오직 점액세포(mucous Cell) 한 종류뿐이 란 보고도 있었다. (중략)

• Applied Microscopy
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• v.20 no.1
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• pp.1-16
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• 1990

The epidermal tissue of Korean planaria Phagocata vivida(Ijima et Kaburaki) is composed of the simple columnar epithelium. The ventral epidermis of this animals is thinner than the dorsal epidermis and has a furrow in the median line in which dark cell is observed. The clear cells which are electron-lucent are located either side of the dark cells. Those are compactly covered with long cilia. The free surface of the latero-ventral epidermis is tightly contacted with the earth and this epidermal free surface has a great number of short cilia, and a lot of C-type of basophilic granule cell are migrated into the cytoplasm of epithelium from mesenchyme passing through the basement membrane and then this granules are put out of latero-ventral free surface. Dorsal epidermis is thickest among the whole epidermis of these animals and the rhabdite granules are more distributed in dorsal epidermis than in ventral epidermis. According to the cytochemical and ultrastructural research, composed epidermis of this planaria are divided into nine type cells, that is, ciliated columnar epithelium, dark cell, mucous cell, rhabdite-forming cell, sunk round cell, A type, B type, C type and D type of basophilic granule cell.

• Applied Microscopy
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• v.48 no.1
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• pp.11-16
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• 2018

For Odontamblyopus lacepedii with small and turbid eyes, the gross structure and histology of the olfactory organ, which is important for its survival and protection of the receptor neuron in estuarial environment and its ecological habit, was investigated using a stereo, light and scanning electron microscopes. Externally, the paired olfactory organs with two nostrils are located identically on each side of the snout. These nostrils are positioned at the anterior tip of the upper lip (anterior nostril) and just below eyes covered with the epidermis (posterior nostril). Internally, this is built of an elongated olfactory chamber and two accessory nasal sacs. In histology, the olfactory chamber is elliptical in shape, and lined by the sensory epithelium and the non-sensory epithelium. The sensory epithelium of a pseudostratified layer consists of olfactory receptor neurons, supporting cells, basal cells and lymphatic cells. The non-sensory epithelium of a stratified layer has swollen stratified epithelial cells and mucous cells with acidic and neutral sulfomucin. From these results, we confirmed the olfactory organ of O. lacepedii is adapted to its ecological habit as well as its habitat with burrows at the muddy field with standing and murky waters.

• The Korean Journal of Zoology
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• v.36 no.2
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• pp.159-174
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• 1993

까치 살모사(AEkistrodon snatilis)의 활동기와 동면기에서 일반적인 혈액성상과 혈액내 가스함량의 변화 또한 위장관 점액세포에 대한 형태적 및 조직화학적 변화 양상을 규명 비교하고자 한다 활동기와 동면기에 까치 살모사의 혈액내 총이산화탄소량(TCO2). 이산화탄소부분압(PCO2), 산소압(PO2)과 pH는 활동기보다 동면기에 증가하였으며, 적혈구수, 혈색소, 적혈구 용적 백분율은 활동기보다 동면기에 약간 증가하는 경향이었다. 또한 백혈구수는 활동기보다 동면기에 뚜렷한 감소를 보였다 활동기와 동면기의 위점막에서는 현저한 차이는 없으나, 소장 점막은 동면기에 그 주름수가 감소하고 높이가 낮아지며 점액세포가 위축되었으며, 위 표면 점액세포는 활동기에 중성 점액질과 약간의 Sialomucin을 포함한 산성 점액질로 구성되었으나 동면기에는 중성 점액질과 Sialomucin이 약간 감소하였다 위점액경세포는 활동기에 중성 점액질만을 포함하고 있었으나. 동면기에는Sialomucin을 포함한 산성 점액질이 소량 검출되었다 소장 점액세포는 활동기에 산성점액질과 중성 점액질로 구성되었으며. 산성 점액질에는 Sulfomucin과 Sialomucirr이 포함되어 있었으나 동면기에는 Sulfomucin과 Sialomucin이 다소 감소하는 경향이었다 주사전자현미경 관찰을 통한 까치 살모사의 위장관 표면 상피세포의 미세구조적 변화는 각동면기 별로 차이를 인정할 수가 있었다. 위장관점 막 상피세포는 활동기에 비해 동면기에 상당한 구조적 위축현상을 보이는 경향을 알 수 있다.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.29 no.1
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• pp.1-15
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• 2016

Objective : For various reasons, Obesity & Rhinitis are constantly rising. So Interest of treatment has been expanding. We want to verify The Chungyeol (fire extinguishing) Lisup (Draining) effect of Bangpungtongsungsan on obese model of allergy rhinitis.Material and Methods : BALB/c mouse were divided four groups: control(CON), allergic induction(ARE), Bangpungtongsungsan extract administration(BTT), Bangpungtongsungsan double concentration extract administration (BT2T). Every group except control group were caused allergic rhinitis by Ovalbumin. BTT & BT2T were orally administered the Bangpungtongsungsan for 21days. Since then we observed the liver tissue cell and the nasal mucous membrane.Results : In comparison with ARE, experimental groups show relief of the nasal mucous membrane damage(secretion of mucus decrease, Itching decrease), Th2 eruption control(IL-4 decline), effect of anti-inflamatory(reducing TNF-α creation, decreasing of iNOS through NF-κB activation-inhibition). In addition, experimental groups show a loss in weight, diminished accumulation of fat. (decreasing within liver tissue, reducing TNF-α creation) BT2T is more effective to BTT.Conclusion : Bangpungtongsungsan treat obese model on allergy rhinitis thereby control fat augmentation, relieving inflammation.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.6
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• pp.547-550
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• 2001

Acinic cell carcinoma is a rare salivary gland carcinoma, usually being found in the parotid gland and is uncommon in the other major and minor salivary glands. The tumor cells consist of either serous or mucous acinar cells with few ductal or myoepithelial cell elements. The tumor is a low-grade malignancy with slow growth potential. Surgical therapy depends on tumor size and the extent of infiltration into neighboring tissues. Superficial parotidectomy or total parotidectomy is the initial method of therapy in case of acinic cell carcinoma on parotid gland. When regional neck lymph nodes are involved, the operation is combined with a neck dissection, or with radiation therapy. In the short follow up period, acinic cell carcinoma has good prognosis with 5 year survival rate after surgery is over 80%. In the long-term follow-up, however, there is a tendency to increase in recurrence or metastasis. We experienced a case of acinic cell carcinoma of parotid gland in a 57-year-old female, so we report it with literatures review.