• The Journal of Advanced Prosthodontics
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• 제6권5호
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• pp.379-386
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• 2014

PURPOSE. These days, mesenchymal stem cells (MSCs) have received worldwide attention because of their potentiality in tissue engineering for implant dentistry. The purpose of this study was to evaluate various growth inducing factors in media for improvement of acquisition of bone marrow mesenchymal stem cells (BMMSCs) and colony forming unit-fibroblast (CFU-F). MATERIALS AND METHODS. The mouse BMMSCs were freshly obtained from female C3H mouse femur and tibia. The cells seeded at the density of $10^6$/dish in media supplemented with different density of fetal bovine serum (FBS), $1{\alpha}$, 25-dihydroxyvitamin (VD3) and recombinant human epidermal growth factor (rhEGF). After 14 days, CFU-F assay was conducted to analyze the cell attachment and proliferation, and moreover for VD3, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was additionally conducted. RESULTS. The cell proliferation was increased with the increase of FBS concentration (P<.05). The cell proliferation was highest at the density of 20 ng/mL rhEGF compared with 0 ng/mL and 200 ng/mL rhEGF (P<.05). For VD3, although the colony number was increased with the increase of its concentration, the difference was not statistically significant (P>.05). CONCLUTION. FBS played the main role in cell attachment and growth, and the growth factor like rhEGF played the additional effect. However, VD3 did not have much efficacy compare with the other two factors. Improvement of the conditions could be adopted to acquire more functional MSCs to apply into bony defect around implants easily.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 국제심포지움
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• pp.88.2-98
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• 2002

A wide arry of naturally occurring substances particularly those present in dietary and medicinal plants, have been reported to possess substantial cancer chemopreventive properties. Certain phytochemicals retain strong antioxidative and anti-inflammatory properties which appear to contribute to their chemopreventive or chemoprotective activities. Inducible cyclooxygenase(COX-2) and nitric oxide synthase (iNOS) are important enzymes that mediate inflammatory processes. There is some evidence that expression of both COX-2 and iNOS is co-regulated by the eukaryotic transcription factor NF-$textsc{k}$B. Increased expression of COX-2 and/or iNOS has been associated with pathophysiology of certain types of human cancers as well as inflammatory diseases. Since inflammation is closely linked to tumor promotion, substances with potent anti-inflammatory activies are anticipated to exert chemopreventive effects on carcinogenesis, particularly in the promotion stage. An example is curcumin, a yellow pigment of turmeric (Curcuma longa L., Zingiberaceae), that strongly occurring diaryl heptanoids structurally related to curcumin have substantial anti-tumor promotional activities in two-stage mouse skin carcinogenesis. Thus, yakuchinone A [1-(4'-hydroxy-3'-methoxyphenyl)-7-phenyl-3heptanone] and yakuchinone B [1-(4'-hydroxy-3'methoxyphenyl)-7-phenylhept-1-en-3-one] present in Alpinia oxyphylla Miquel (Zingiberacease) attenuate phorbol ester-induced inflammation and papilloma formation in female ICR mice. These diarylheptanoids also suppressed phorbol ester-induced activation of epdermal ornithine decarboxylase and its mRNA expression when applied onto shaven backs of mice. Yakuchinone A and B as well as curcumin inhibited phorbol ester-induced expression of COX-2 and iNOS and their mRNA in mouse skin via inactivation of NF-$textsc{k}$B. Capsaicin, a major pungent ingredient of red pepper also attenuated phorbol ester-induced NF-$textsc{k}$B activation. Similar suppression of COX-2 and iNOS and down-regulation of NF-$textsc{k}$B activation for its DNA binding were observed with the ginsenosied Rg3 and the ethanol extract of Artemisia asiatica. We have also found that certain anti-inflammatory phytochemicals exert inhibitory effects on phorbol ester-induced COX-2 expression and NF-$textsc{k}$B activation in immortalized human breast epithelial (MCF-10A) cells in culture. One of the plausible mechanisms undelying inhibition by aforementioned phytochemicals of phorbol ester-induced NF-$textsc{k}$B activation involves interference with degragation of the inhibitory unit, I$textsc{k}$Ba, which blocks subsequent nuclear translocation of the functionally active p65 subunit of NF-$textsc{k}$B. the activation of epidermal NF-$textsc{k}$B by phorbol ester and subsequent induction of COX-2 hence appear to play an important role in intracellular signaling pathwasy leading to tumor promotion and targeted inhibition of NF-$textsc{k}$B may provide a new promising cancer chemopreventive strategy.

• Asian Pacific Journal of Cancer Prevention
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• 제15권7호
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• pp.3057-3063
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• 2014

Background: Anaplastic lymphoma kinase (ALK) gene rearrangement in non-small cell lung cancer (NSCLC) has been intensively studied. The gold standard for ALK detection is FISH, but this is not routinely conducted in clinical practice, so that the IHC method has a role. The aim of this study was to identify the incidence of ALK rearrangement and risk or prognostic factors for ALK positivity using both of IHC and FISH methods. Materials and Methods: From January 2008 to December 2012, 267 completely resected NSCLC patients in Chiang Mai University Hospital were enrolled in this study. Clinical and pathological variables and outcomes of treatment were retrospectively reviewed. IHC and FISH were used to evaluate ALK rearrangement. Sensitivity and specificity of IHC were analyzed. Multivariable analysis was used to identify clinico-pathological correlations with positive results of IHC and clinical outcomes. Results: Twenty-two (8.2%) of 267 specimens were IHC-positive for ALK with intense cytoplasmic staining, whereas only 10 (3.8%) were FISH-positive. Sensitivity, specificity and the positive likelihood ratio with IHC were 80.0%, 94.9%, and 15.8 respectively. Age less than 55 years (RR 4.4, 95%CI 1.78-10.73, p value=0.001) and presence of visceral pleural invasion (VPI) (RR 2.9, 95%CI 1.21-6.78, p value =0.017) were identified as risk factors for ALK rearrangement with FISH. There were no statistically significant differences in other clinical and pathological variables. ALK rearrangement was not a prognostic factor for tumor recurrence or overall survival. Conclusions: The incidences of ALK positivity in completely resected NSCLCs in northern Thailand were 8.2% by IHC and 3.8% by FISH. IHC with mouse monoclonal, Ventana D5F3 antibody can be used as a screening tool before FISH method because of high specificity and high positive likelihood ratio. Age less than 55 years and VPI are risk factors for ALK positivity.

• 한국가축번식학회지
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• 제19권2호
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• pp.95-104
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• 1995

Mouse mammary epithelial cells(NMuMG) were maintained onto 6-well plates (3$\times$105 cells/well) or chambered slide (1$\times$104 cells/well), in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 hours, DMNB (1$\mu$M) was added and exposed to UV light (300nm, 3 second pulse) after 2 hours from DMNB addition in order to activate DMNB which induces a rapid transient increase in intracellular cAMP upon UV irradiation. EGF (100ng/ml) and/or IGF-I (10ng/ml) were treated at the time of UV irradiation. Nuclear labeling index was estimated as percent of nuclear labeled cells(percent of S phase of cells) by incorporation of 3H-thymidine into DNA(1 hour pulse with 1$\mu$Ci/ml). DMNB(1$\mu$M), EGF (100ng/ml) and/or IGF-I (10ng/ml) signifciantly increased nuclear labeling index than those of control (P<0.05). Addition of DMNB＋EGF or DMNB＋EGF＋IGF-I showed the interaction effect in nuclear labeling index (P<0.05). Protein kinase A activities by addition of EGF, IGF-I or EGF＋IGF-I were 10.5, 9.8 or 9.4 unit/mg protein, respectively, and no statistical difference was found in comparison with control (P>0.05). Additon of DMNB＋EGF showed the moderate interaction effect on tyrosyl kinase activity (P<0.1). In the fluorography analysis, there were no specific protein phosphorylation patterns were found at 1 or 15 minute by addition of DMNB. EGF and/or IGF-I. These results suggest that the interaction effect in nuclear labeling index by addition DMNB and EGF could be mediated through the modulation of tyrosyl kinase activity by cAMP.

• 한국항행학회논문지
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• 제17권2호
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• pp.240-246
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• 2013

본 연구는 터치스크린 방식의 정보전달 기기인 DID 시스템에 Airtouch 기술을 융합하여 새로운 방식의 정보전달 시스템을 개발하는 것이다. 대학의 학내 공지사항, 교육정보 및 취업정보 등을 열람하고 활용 할 수 있는 시스템을 설계하고 Airtouch 기술을 구현하여 원격조작 및 공유 콘텐츠 스토어, 클라우드 서비스 개발을 통한 콘텐츠 동기화 기술 구현으로 스마트융합 DID 시스템을 개발하고자 한다. 키넥트는 USB 인터페이스를 가지고 있기 때문에 정보기기와 연결하여 사용 할 수 있으며, 저렴한 가격의 제품으로 키넥트 센서를 활용하여 Airtouch 기술을 구현한다. 논문에서는 유형의 입력장치 없이 사용자의 손동작만으로 정보기기와 상호작용이 가능한 시스템인 Airtouch 기술을 활용한 스마트융합 DID 시스템을 개발하여 사용자의 손 움직임을 추적하여 이를 바탕으로 마우스 포인터를 조작할 수 있고, 사용자의 손동작을 통해 정보기기에 명령을 내릴 수 있도록 하였다. Airtouch 기술을 활용한 스마트융합 DID 시스템 기술 활용으로 온라인 교육산업, 광고홍보, 정보화 산업 등 여타 산업에 파급효과를 증대시킨다. 또한, 폭넓은 범용성을 가지고 기존의 인터페이스 장치들을 대체기술 활용성이 무한 확장이 가능하다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.121-124
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• 2002

This work was initiated to develope a recombinant oral poliovaccine (OPV), which is highly advanced in safety (minimizing VAPP) by introducing Type 2,3 poliovirus epitopes into our RPS-Vax system. We have introduced several potential vaccine epitopes of poliovirus Type 2, and 3 into RPS-Vax system, resulting in production of recombinant polioviruses. Any of these chimeric viruses, however, were not detected for their foreign gene expression by serotype-specific mouse antiserum. We have designed several folding units to stabilize the introduced vaccine protein and attached short epitope-concatamer or epitope-multimer to them, followed by production of chimeric viruses. Only those who have an HIV-1 Tat-mediated folding unit were nicely detected for the introduced foreign proteins by anti-Tat antiserum and type-specific peptide-induced antisera. Nevertheless, introduced epitopes were not detected in Western blot experiment with each serotype-specific antiserum. None of the mice inoculated with these chimeric viruses showed preventative immunity when challenged with Lansing and Leon wildtype 2 and 3 poliovirus, and the antiserum did not show neutralizing capacity in vitro. Conformational epitope covering B/C loop region of type 2 and 3 were newly designed by computer modeling, and introduced into the RPS-Vax vector system, followed by production of chimeric viruses. Introduced epitope regions were nicely detected by anti-Tag23 mAb or peptide antibody, but still not detected by poliovirus antiserum. Nevertheless, neutralizing antibody was detected in the Tg-PVR mice even when inoculated once with these chimeric viruses. Also, the immunized mice showed perfect preventative immunity against the wild Type poliovirus Lancing or Leon. When boosted appropriately, those chimeric virus-inoculated Tg-PVR mice produced equivalent amounts of neutralizing antibody to those in Sabin 2/3-immunized mice. These data strongly suggest that our recombinant poliovirus (RPS-PV2 and RPS-PV3) can be used as a safe and effective rec-OPV instead of any preexisting poliovaccine.

• 한국해양학회지
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• 제23권3호
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• pp.123-129
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• 1988

본 연구는 국내산 각종 이매패류의 마비성패독에 의한 독화상황을 조사한 것이다. 조사된 28종의 이매패 중에서 진주담치(Mytiluks edulis galloprovincialis), 홍합(M. corsucus), 큰 가리비(Patinopecten yessoensis), 비단가리비(Chlamys farreri), 비단조개(Peronidia venulosa) 및 반지락(Ruditapes philippinarum)에서 독성이 확인되었다. 지역적으로는 전 연안의 패류에서 독성이 확인되기는 하였으나, 주로 남해 동부역의 진동만 부근에서 독화현상이 관찰되고 있고, 동해안의 포항에서도 독성이 확인되었다. 독화하는 시기는 포항의 경우 5월-8월 이었으나, 그 밖의 연안역에서는 주로 4월-6월 이었으며, 독성은 전반적으로 외국에서의 허용한계값인 4 MU/g 가식부를 초과하지는 않았으나, 진동만과 철천수로 및 포항의 시료에서 이를 약간 상회하는 독량이 검출되기도 하였다.

• Biomolecules & Therapeutics
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• 제5권4호
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• pp.344-350
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• 1997

The purpose of the present study was to produce and characterize a monoclonal antibody against human ${\beta}_2$-adrenergic receptor. Male BALB/c mice were immunized with glutathione S-transferase (GST) fusion protein of the C-terminal portion of the human ${\beta}_2$-adrenergic receptor which was expressed in E.Coli. The immunized splenocytes were fused with myeloma SP2/0-Agl4 cells. The resulting hybridomas were screened for the production of a monoclonal antibody which can recognize human ${\beta}_2$-adrenergic receptor, and then subcloned by limiting dilution. The resulting monoclonal antibody was named as mAb$\beta$CO2. The mono-clonal antibody $\beta$CO2 was determined as IgM subtype and then purified by anti-mouse IgM-agarose affinity chromatography. The results of ELISA, Western blot, and immunocytochemistry showed that mAb$\beta$CO2 recognized human ${\beta}_2$-adrenergic receptor in the ${\beta}_2$-adrenergic receptor-GST fusion protein and human spider-moid carcinoma cell line A431 with highly specific immunoreactivity, The monoclonal antibody $\beta$CO2 may provide useful tools for the study of the $\beta$-adrenergic receptor of human and other species including rats.

• 한국식품영양과학회지
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• 제18권3호
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• pp.273-278
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• 1989

해동피 methanol 추출물을 시료로 mouse의 급성독성실험, 실험적으로 유발시킨 가토의 간장기능 장애에 미치는 영향을 실험하여 다음과 같은 결과를 얻었다. 실험결과 해동피 methanol 추출물의 급성독성 $(LD_{50})=7.3g/kg$으로 무독하였고 $CCI_4$로 손상시킨 가토의 간장기능회복에 있어서는 실험동물체중 kg당 시료 800mg투여군에서 14일째 대조군에 비해 SGPT가 $87.28{\pm}23.03$ unit로 서서히 유의성있게 회복되었다. 시료 800mg/kg투여군에서 alkaline phosphatase 활성도는 2일째부터, 그리고 total bilirubin 측정치는 6일째부터 현저히 회복되는 경향을 보였다. 특히 total cholesterol 측정치는 200mg/kg투여군에서 14일째 $99.36{\pm}12.42mg/d{\ell}$과 800mg/kg투여군에서 10일째 $96.69{\pm}18.40mg/d{\ell}$, 14일째 $80.68{\pm}8.77mg/d{\ell}$로 대조군에 비해 현저한 감소를 보였다.

• 한국정보통신학회논문지
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• 제15권3호
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• pp.700-712
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• 2011

현대의 인간은 시간과 공간에 상관없이 정보기기를 이용하여 네트워크에 접근할 수 있는 환경에 노출되어 있다. 이러한 환경에서 사용자들은 다수의 온라인 정보 기기들을 통해 정보를 수신, 입력, 가공이 가능하다. 하지만, 현대에 널리 사용되는 마우스, 조이스틱, 트랙볼 등과 같은 포인팅 시스템은 휴대가 불편하고, 사용시 손이 자유롭지 않기에 유비쿼터스 환경에서 사용하기 어렵다. 본 논문에서는 휴대 및 설치가 간편하고, 사용시 사용자의 손이 자유로운 무선 센서기반의 다차원 사용자 움직임 탐지 시스템을 구현하였다. 본 시스템은 사용자 팔 움직임을 감지하여 수신기로 전달하는 입력기와 입력기로부터 받은 데이터를 응용프로그램으로 전달하는 수신기, 수신한 데이터를 처리하여 명령을 수행하는 응용프로그램으로 구성된다. 실험을 통하여 제안된 시스템이 사용자의 팔 움직임을 정확히 탐지하고, 해당된 입력 요구사항을 충분히 수행함을 보인다.