• Proceedings of the KIEE Conference
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• 2006.07d
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• pp.1821-1823
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• 2006

This paper presents a development of interface device for electro-oculogram(EOG) signal and it's application to the wireless mouse of wearable PC. The interface device is composed of five bio-electrodes for detecting oculomotor motion, several band-pass filters, instrumentation amplifier and a microprocessor. we have first analyzed impedance characteristics between skin and a bio-electrode. since the impedance highly depends on human face, it's magnitude differs from person. this interface device was applied to develop a wireless mouse for wearable PC, as a Bio Machine Interface(BMI). Where in the prompt on PC monitor is controlled by only EOG signals. this system was implemented in a Head Mount Display(HMD) unit. experimental results show the accuracy of above 90%.

• Journal of the Ergonomics Society of Korea
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• v.19 no.3
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• pp.39-49
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• 2000

During human-computer interaction(HCI), people typically send inputs to computers through electromechanical pointing devices. Many applied studies have therefore evaluated cursor-positioning movements made with various pointing devices. Though there were so many studies about performance of various pointing devices, it was nearly impossible to compare device performance each other until the Fitts' law was applied. It does appear that Fitts' law may predict performance reasonably well for the one C-D gain level. But in varying C-D gain levels, Fitts' law could not predict movement time. This study investigated the effects of C-D gain in mouse movement time and suggested a revised Fitts' model including C-D gain as an independent variable. The revised Fitts' model may use to measure the performance of various devices in varying C-D gain levels.

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.196.1-196
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• 1997

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.176.2-176
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• 1999

No Abstract, See Full Text

• BMB Reports
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• v.38 no.3
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• pp.290-293
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• 2005

In order to investigate the epitope of basic fibroblast growth factor (bFGF) and its immunogenicity, the epitopes of bFGF were screened from the phage display library with monoclonal antibody GF22, which can neutralize the bio-activity of bFGF. By three rounds of screening, the positive phage clones with bFGF epitopes were selected, which can effectively block the bFGF to bind with GF22. Sequence analysis showed that the epitopes shared a highly conservative sequence (Leu-Pro-Pro/Leu-Gly-His-Phe/Ile-Lys). The sequence of PPGHFK was located at 22-27 of the bFGF. The specific immuno-response of mouse could be highly induced by phage clones with the epitopes. And the anti-bFGF activity induced by LPGHFK was 3 times higher than the original sequence, which showed that the mimetic peptide LPLGHIK might be used as a tumor vaccine in the prevention and treatment of tumor.

• IMMUNE NETWORK
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• v.3 no.3
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• pp.219-226
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• 2003

Background: Phage display is the most widely used technique among display methods to produce monoclonal antibody fragment with a specific binding activity. Having a large library for efficient antibody display/selection is quite laborious process to have more than $10^9$ members of transformants. To overcome these limitations, several in vitro selection approaches have been reported. Ribosome display that links phenotypes, proteins, directly to genotype, mRNA, is one of the in vitro display methods. Ribosome display can reach the size of scFv library up to $10^{14}$ molecules and it can be further diversified during PCR steps. To select the high affinity scFv from one pot library, we established ribosome display technique by modifying the previously reported eukaryotic translation system. Methods: To establish the antibody selection system by ribosome display, we used 3D8, anti-DNA antibody. A 3D8 scFv was synthesized in vitro by an in vitro transcription-translation system. The translated 3D8 scFv and the encoding 3D8 mRNA are connected to the ribosome. These scFv-ribosome-mRNA complexes were selected by binding to their specific antigens. The eluted mRNAs from the complexes are reverse transcribed and re-amplified by PCR. To apply this system, antibody library from immunized mouse with terminal protein (TP)-peptide of hepatitis B virus DNA polymerase TP domain was also used. This TP-peptide encompasses the 57~80 amino acid residues of TP. These mRNA/ribosome/scFv complexes by our system were panned three times against TP-peptide. The enrichment of antibody from library was determined by radioimmunoassay. Results: We specifically selected 3D8, anti-DNA antibody, against ssDNA as a model system. The selected 3D8 RNAs sequences from translation complexes were recovered by RT-PCR. By applying this model system, we enriched TP-peptide-specific scFv pools through three cycles of panning from immunized library. Conclusion: We show that our translating ribosome complexes are well maintained and we can enrich the TP-specific scFv pools. This system can be applied to select specific antibody from an antibody library.

• Journal of Korea Game Society
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• v.10 no.2
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• pp.29-36
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• 2010

As more and more advanced technology implemented in Computer Game, various of technical skill must be applied to satisfy the demand of the users specifically in the immersion and convenience of the games. As a result, The number of interactive games, which are controlled by user's physical action so that make the games more immersible, are increasing. In Addition, as the amount of information that happens in the games increases, many studies are going on to provide efficient user-interface. In this study, we develop an interactive game using a controller designed by us, which consist of Wii controller and an attached secondary display that provides game information to user. In addition, we conduct a survey with questions about immersion and convenience of the game to understand how users feel when doing the suggested game, and to compare our game environment with general FPS game environment that uses mouse and keyboard.

• The Journal of the Korea Contents Association
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• v.9 no.6
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• pp.117-125
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• 2009

In this paper, we propose user-centric immersible and interactive electronic book based on the interface of tabletop display. Electronic book is usually used for users that want to read the text book with multimedia contents like video, audio, animation and etc. It is based on tabletop display platform then the conventional input device like keyboard and mouse is not essentially needed. Users can interact with the contents based on the gestures defined for the interface of tabletop display using hand finger touches then it gives superior and effective interface for users to use the electronic book interestingly. This interface supports multiple users then it gives more diverse effects on the conventional electronic contents just made for one user. In this paper our method gives new way for the conventional electronics book and it can define the user-centric gestures and help users to interact with the book easily. We expect our method can be utilized for many edutainment contents.

• Development and Reproduction
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• v.9 no.1
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• pp.33-41
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• 2005

Identification of differentially expressed genes at blastocyst stage embryos would provide insights into early development and differentiation. Here, we applied a new differential display reverse transcription polymerase chain reaction(DD RT-PCR) technology, called annealing control primers(ACP) system to identify the genes that are specifically or prominently expressed in mouse blastocysts compared to embryonic stem(ES) cells. Using 100 ACPs, 26 clones were perceived as differentially expressed genes in mouse blastocysts. A BLAST search revealed that cloned genes had significant sequence similarities with known genes in the GenBank/EMBL data base. Among them, 15 genes were selected and conformed by RT-PCR. This analysis suggests that the ACP system is a practical method for the identification of stage-specific genes using small numbers of mouse embryos.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.48-48
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• 2001

A new neurological mutant has been found in the ICR outbred strain mouse. Affected mice display profound deafness and a head-tossing and bidirectional circling behavior, showing an autosomal recessive mode of inheritance. It was, therefore, named cir/Kr with the gene symbol cir. The auditory tests identified clearly the hearing loss of the cir mice when compared to wild type mice. Pathological studies confirmed the developmental defects in the middle ear, cochlea, cochlear nerve, and semicircular canal areas, which were correlated to the abnormal behavior observed in the cir mice. Thus, cir mice may be useful as a model for studying inner ear abnormalities and deafness. We have constructed a genetic linkage map by positioning 14 microsatellite markers across the (cir) region and intraspecific backcross between cir and C57BL/6J mice. The cir mouse harbors an autosomal recessive mutation on mouse chromosome 9. The cir gene was mapped to a region between D9Mit116 and D9Mit38 Estimated distances between cir and D9Mit116, and between cir and D9Mit38 are 0.7 and 0.2 cM, respectively. The gene in order was defines : centromere-D9Mit182-D9Mit51/D9Mit79/D9Mit310-D9Mit212/D9Mit184-D9Mit116-cir-D9Mit38-D9Mit20-D9Mit243-D9Mit16-D9Mit55/D9Mit125-D9Mit281. The mouse map location of the cir locus appears to be in a region homologous to human 3q21. Our present date suggest that the nearest flanking marker D9Mit38 provides a useful anchor for the isolation of the cir gene in a yeast artificial chromosome contig.