• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.199-206
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• 1994

We present here a new PCR-based cloning technique that allows the different PCR products during mouse embryogenesis. Recently, mRNA differential display described by Liang & Pardee (Science 257, 1992) and re-confirmed by Zimermann & Schultz (PNAS 91,1994). This method will detect the appropriate changes in the temporal patterns of expression or in the transition from maternal control to zygotic control as well as the functional difference of embryo with polyspermy or monospermy, the difference of expression between successfully hatched blastocyst and blastocyst failed to hatching, response to agents, and cell cycle regulation. By this methods, we have cloned an eDNA, which showed mouse 2 cell specific expression. Genomic DNA digested with EcoRI showed approximately 15 kb and then showed higher expression in fetal liver rather than adult liver. Furthermore, this gene is likely to have 2 mRNA by alternative splicing.

• BMB Reports
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• v.41 no.12
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• pp.875-880
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• 2008

Mbu-3 is a novel mouse brain unigene that was identified by digital differential display. In this study, expression of the gene was chased through developmental stages and the protein product was identified in the brain. The cDNA sequence was 3,995-bp long and contained an ORF of 745 AA. Database searches revealed that the chicken SST273 gene containing LRR- and Ig-domain was an mbu-3 orthologue. Tissue specificity for the gene was examined in embryos and in brains at post-natal and adult stages. During the embryonic stages, mbu-3 was localized to the central nervous system in the brain and spinal cord. In the early post-natal stages, the gene was evenly expressed in the brain. However, with aging, expression was confined to specific regions, particularly the hippocampus. The protein was approximately 95 kDa as determined by Western blot analysis of brain extracts.

• Journal of the Korea Society of Computer and Information
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• v.12 no.2 s.46
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• pp.161-170
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• 2007

This paper is a matter of remote control apparatus and display remote control system comprising the same. The remote controller is a wireless remote pointing device that is included a function of mouse and a function of remote controller for VOD etc. It is assembled a sending signal part to communicate with display device, a image sensor part to capture a scene of front view and a main body. Also, it includes a control part that analyzes the captured image, calculates a relative moving factor that main body is moved by user, and then controls the mouse cursor on the display device. The remote control apparatus is able to capture a seen of front view, to analyze the captured image, and to move a mouse cursor point on the display device as much as it calculated relative moving factor. Therefor it brings don the cost of production by a product desist that is increased user satisfaction. And it, using the patent and the new product desist, will make an epoch in development of the remote control system for Ubiquitous broadcasting industry.

• BMB Reports
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• v.29 no.3
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• pp.272-275
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• 1996

The expression of genes induced by Dichloroacetate (DCA) treatment was analyzed by mRNA differential display. Purified total RNAs from rat liver treated with saline or DCA (100 mg/100 g b.w.) were reverse transcribed by using a set of oligonucleotide primers. The PCR products were resolved on a denaturing sequencing gel. PCR band representing mRNA expressed specifically in DCA-treated liver was excised and reamplified by PCR. A 120-bp c-DNA clone named IC1 was isolated and the DNA sequence of IC1 was analyzed. IC1 revealed 50% homology with 3' end of a mouse fibroblast growth factor mRNA This result indicates that DCA induces the expression of a gene which has a 50% homology with a Mouse fibroblast growth factor, and expression of this gene might be involved in non genotoxic process caused by DCA.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.211.1-211.1
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• 2016

Several reports have demonstrated the wide range of nonthermal plasma applications in biomedical field including cancers, diabetics, wound healing and cosmetics. Recently, it has been shown that plasma is able to modulate the p38 MAPK and JUN level in cells which has a crucial role in melanin synthesis and skin pigmentation. Therefore we investigated the effect of plasma on melanogenesis in-vitro using melanoma (B16F10) cells and in-vivo using mouse and zebra fish. To investigate the mechanism of plasma action, plasma device characteristics were measured, reactive species inside and outside the cells were detected, and western blot was performed to find the signaling pathway involved in melanin activation in-vitro and in-vivo. This is the first report presenting the role of nonthermal plasma for melanogenesis which provides a new perspective of plasma in the field of dermatology.

• Biomedical Science Letters
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• v.10 no.3
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• pp.211-217
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• 2004

For discrimination of ductal and ascinar cells, we isolated a single-chain variable domain fragment (scFv) antibody against ductal cells of salivary gland using phage display technique. From the spleen of a mouse immunized with ductal cell lysate, total RNA was prepared and used as a template for cDNA synthesis of antibody genes. The scFv genes were constructed with variable domain genes of heavy and light chain and were introduced into pCANTAB5E to construct phage scFv library. The phage particles specific for acinar cells were screened by subtraction using immunotubes coated with acinar and ductal cell lysate and enzyme-linked immunoabsorbance assay (ELISA). The characteristics of the scFv were determined by immunohistochemistry (IHC) and the result indicated that the isolated scFv has the specificity against ductal cells of salivary glands and tubules of kidney. And the scFv has an unique binding activity specific for Hashimoto's thyroiditis. The nucleotide sequence of isolated scFv gene was determined and revealed that V/sub H/ belongs to the mouse H-chain family subgroup IB and V/sub L/ to the mouse L-chain family subgroup III.

• Journal of the Korean Society of Safety
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• v.23 no.2
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• pp.30-36
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• 2008

This paper suggests the best work conditions, including short break time and number of mouse clicks on a computer, to prevent MSDs(Musculoskeletal Disorders) on VDT(Visual Display Terminal) work. Discomfort measures are calculated according to the Borg's CR-10 Scale. The short break time conditions are grouped into 7, 15, and 30 seconds after every 10-minute work period and the number of mouse clicks are divided into 10 clicks/min, 20clicks/min, and 30clicks/min. The result of the ANOVA on the shift value of subjective discomfort shows the following: 1) Regarding the break time and the number of mouse clicks, there are statistical differences between the measured values for the neck and the wrist(p<0.05). 2) Regarding the number of mouse clicks, there are statistical differences between the measured values for the shoulder and the forearm(p<0.1). 3) Regarding the break time and the number of mouse clicks, there are no statistical differences between the measures values for the eyes, upper arms and back(p<0.1).

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.79-79
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• 2000

In this Paper, the PC input device using MEMS gyros and accelerometer is newly developed, so that it can measure rotation rate and linear acceleration of the human body in space. In General, the human motion has 6 degree of freedom but 2 degree of freedom is enough PC monitor with 2D display. Therefore the simple method is proposed to achieve minimum degree of freedom. It is also applied to the PC mouse. This method can be expanded to the input device for internet set-top box or internet TV.

• Parasites, Hosts and Diseases
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• v.44 no.1 s.137
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• pp.15-20
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• 2006

Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive brain passages, we examined the genes induced in mouse brain-passaged A. culbertsoni by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR). Enhanced A. culbertsoni virulence was observed during the second mouse brain passage, i.e., infected mouse mortality increased from $5\%\;to\;70\%.$ Ten cDNAs induced during mouse brain passage were identified by DDRT-PCR and this was confirmed by northern blot analysis. BlastX searches of these cDNAs indicated the upregulations of genes encoding predictive NADH-dehydrogenase, proteasomal ATPase, and GDP-mannose pyrophosphorylase B, which have previously been reported to be associated with A. culbertsoni virulence factors.

• Journal of Institute of Control, Robotics and Systems
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• v.11 no.5
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• pp.445-450
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• 2005

In this paper, we suggest an integrated tactile display system that provides kinesthetic force, pressure distribution, vibration and slip/stretch. The system consists of two parts: a 2 DOF force feedback device for kinesthetic display and a tactile feedback device for displaying the normal stimulation to the skin and the skin slip/stretch. Psychophysical experiments measure the effects of fingerpad selection, the direction of finger movements and the texture width on tactile sensitivity. We also investigate the characteristics of lateral finger movement while subjects perceive different textures. From the experimental results, the principal parameters for designing a tactile display are suggested. A tactile display device, using eight piezoelectric bimorphs and a linear actuator, Is implemented and attached to a 2 DOF translational force feedback device to simultaneously simulate the texture and stiffness of the object. As a result, we find out that the capability of the suggested device is sufficient to display physical quantities to display the texture.