• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.1
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• pp.23-33
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• 2007

A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation($-196^{\circ}C$) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.

• Journal of Korean Society on Water Environment
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• v.32 no.2
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• pp.205-215
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• 2016

In order to improve water quality in the Paldang Lake, a riparian vegetation belt, treatment wetland, and artificial floating island were designed for introduction in the upland field, the estuary of tributaries, and the section of water facing mountainous land, respectively. We synthesized vegetation information collected from a reference river and found that herbaceous, shrubby, and tree vegetation zones tended to be dominated by Phragmites japonica, Phalaris arundinacea, etc.; Salix gracilistyla, S. integra, etc.; and S. koreensis, S. subfragilis, and Morus alba, respectively. In our plan, the herbaceous vegetation zone, which is established on floodplains with a high frequency of disturbance, will be left in its natural state. A shrubby vegetation zone will be created by imitating the species composition of the reference river in the ecotone between floodplain and embankment. A tree vegetation zone will be created by imitating species composition on the embankment slope. In the treatment wetland, we plan to create emerged and softwood plant zones by imitating the species composition of the Zizania latifolia community, the Typha orientalis community, the P. communis community, the S. integra community, and the S. koreensis community. The floating island will be created by restoring Z. latifolia and T. orientalis for water purification purposes.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.272-286
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• 2022

Black root rot (BRR) caused by Lasiodiplodia theobromae is an alarming disease of mulberry that causes tremendous economic losses to sericulture farmers in India and China. Successful control of this disease can be attained by screening germplasm and identifying resistant sources. Seventy four diseased root samples were collected from farmer's fields belonging to four major mulberry growing states of South India. Based on morpho-cultural and scanning electron microscopy studies, 57 fungal isolates were characterized and identified as L. theobromae. Phylogenetic analysis of concatenated internal transcribed spacer and β-tubulin sequences revealed variation of the representative 20 isolates of L. theobromae. Following the root dip method of inoculation, pathogenicity studies on susceptible mulberry genotypes (Victory-1 and Thailand male) recognized the virulent isolate MRR-142. Accordingly, MRR-142 isolate was used to evaluate resistance on a set of 45 diverse mulberry accessions. In the repeated experiments, the mulberry accession ME-0168 which is an Indonesian origin belonging to Morus latifolia was found to be highly resistant consistently against BRR. Eight accessions (G2, ME-0006, ME-0011, ME-0093, MI-0006, MI-0291, MI-0489, and MI-0501) were found to be resistant. These promising resistant resources may be exploited in mulberry breeding for developing BRR resistant varieties and to develop mapping populations which successively helps in the identification of molecular markers associated with BRR.