• Journal of Forest and Environmental Science
• /
• 제38권4호
• /
• pp.284-289
• /
• 2022

This study was conducted to determine the seed dormancy types of Sambucus racemosa subsp. pendula Nakai and S. williamsii Hance. Low temperature stratification (1, 2, 3, 4 months) and GA3 treatment (1,000 mg/L) were performed on seeds to determine the type of seed dormancy. After the treatment, seeds were placed on a petri dish at 25℃ under light conditions. The germination rate and mean germination time were investigated. Results showed that cold stratification was effective in breaking the dormant state of the seed in both species. In the low temperature stratification treatment, the seed germination performance was improved as the treatment period was prolonged. Gibberellin treatment was effective in breaking the dormant state of S. racemosa subsp. pendula without low temperature stratification. However, S. williamsii did not break the dormant state of the seed by gibberellin treatment without low temperature stratification treatment. In the gibberellin treatment, germination performance was improved according to the low temperature stratification treatment period. As a result of this study, the seeds of S. williamsii have both an intermediate complex and a deep complex morphophysiological dormancy (MPD). In comparison, it was found that the S. racemosa subsp. pendula had intermediate composite MPD.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 춘계학술발표회
• /
• pp.60-60
• /
• 2018

This study was carried out to develop the seed propagation method of Dicenta spectabilis (L.) Lem. which is an ornamental plant native to Korea. In the previous studies, it was found that the seeds of D. spectabilis were morphophysiologically dormant (MPD), and high and low temperature of stratification were continuously required for the embryo growth and germination of the seeds. Especially, it was most effective to store for 1 month at $20^{\circ}C$ and then to transfer to $4^{\circ}C$. The treatment of $GA_3$ was carried out to promote embryo development and germination. The seeds were submerged in 100, 200 or $500mg{\cdot}L^{-1}$ $GA_3$ for 72 hours and then stored at various conditions as follow. The temperature conditions disposed of this experiment were 1 month at 10, 15, 20, and $25^{\circ}C$ or 2, 4, 8, and 12 months at $4^{\circ}C$, respectively. As a result, the length of embryo and germination rate of the seeds were the best when stored at $4^{\circ}C$ for 8 months after $500mg{\cdot}L^{-1}$ $GA_3$ treatment. Besides, when the seeds stored at $4^{\circ}C$, significant differences in embryo length and germination rate were shown with $GA_3$ concentration and storage period. It was also proved that high-concentration of $GA_3$ could replace the high temperature and could promote germination. Consequentially, the D. spectabilis seeds were classified into intermediate simple levels among MPD types.

• 한국자원식물학회지
• /
• 제29권4호
• /
• pp.385-392
• /
• 2016

본 연구는 삼백초 종자의 체계적인 발아연구를 통한 대량의 실생번식법 확립 및 약용작물로써의 활용도를 높이기 위하여 수행되었다. 채종 후 정선이 완료된 종자는 즉시 휴면유형 분류를 위하여 배 관찰, 직파 발아율 및 불투수성 검정을 실시하였다. 이후 종자를 4 ± 1.0℃에 건조저장하면서 발아특성 연구에 사용하였다. 발아촉진을 유도하고자 생장조절제와 무기염류의 종류 및 농도를 달리한 용액에 24시간 침지처리 하였다. 연구의 결과, 저온처리 되지 않은 삼백초 종자는 분화가 불완전한 미숙배를 가지고 있었으며, 5% 미만의 낮은 발아율을 기록하였다. 또한 단순 침지처리에 의해 수분흡수가 원활하였으며, 형태적으로 미숙한 배가 신장 후 발아되었기 때문에 형태·생리적 휴면으로 생각되었다. 삼백초 종자는 생장조절제와 무기염류 처리에 의해 유의적인 발아촉진 효과가 확인되었으며, 특히 GA₃ 500 ㎎/L 처리구에서 발아세(GE)가 46.1%로 가장 높았다. 또한 KNO₃ 10 ~ 20 mM 처리구에서는 최종발아율(PG)이 54.3 ∼ 57.7% 수준으로 향상되었으므로, 균일한 육묘를 위한 생장조절제와 무기염류의 선택적 이용이 가능할 것으로 생각된다.