• Toxicological Research
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• 제7권1호
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• pp.83-92
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• 1991

Complexities of testis structure and function are emphasized in morphometrical and genotoxic evaluation by statistical analysis. F-344 rats were treated with azinphos methyl, cyclophosphomide, and dichlorvos. And Brdu was injected with intrapertionially before sacrifice. The existence and degree of DNA damage were measured by Brdu labeling index which represented relative amount of Brdu incorporated in DNA, morphometric change was evaluated by the relative length of tubular diameter in circular seminiferous tubules and the number of spermatogonia per Sertoli cell in stage IX seminiferous tubules.

• 한국발생생물학회지:발생과생식
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• 제18권1호
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• pp.33-41
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• 2014

We investigated the process of yolk absorption in Korean rose bitterling, Rhodeus uyekii, and determined the changes in its morphometric characteristics. The R. uyekii from 1 days post hatching (DPH) to 21 DPH, the eye diameter (ED) was 5.4 at 5 DPH. Thereafter, the ED/total length (TL) ratio increased to 10.7 at 21 DPH (p<0.05). The yolk length (YL) decreased from 95.4 to 1.1 by 21 DPH, and this rate of decrease was greater than that for any other dimension (p<0.05). 12 morphometric dimensions/TL for the R. uyekii were measured at each sampling day from 21 DPH to 170 DPH. At just hatching, the average TL and BW were $6.1{\pm}0.09mm$ and $4.9{\pm}0.07mg$, respectively. At 53 DPH, the average TL was $12.9{\pm}0.28mm$ and the average BW was $14.7{\pm}0.72mg$; the total length growth equation was $TL=5.507e^{0.038t}$ ($R^2=0.916$). Further, the body weight growth equation was $BW=3.3647e^{0.0296t}$ ($R^2=0.9354$). The dimensions with regard to body depth showed the greatest growth rates in the external characteristics of the fish (p<0.05). The patterns of the morphometric characteristics measured in this study can be classified in three ways. The patterns were shown to be increased (y=0.0992x+12.07, $R^2=0.8333$), decreased (y=-0.0569x+42.029, $R^2=0.8395$) or maintained (y=0.005x+18.263, $R^2=0.3678$) from 21 DPH to 170. These results will provide useful indices for the successful rearing of the R. uyekii.

• Journal of Korean Neurosurgical Society
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• 제37권6호
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• pp.410-415
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• 2005

Objective: The aim of our study is to evaluate the effectiveness of unilateral hemilaminectomy for bilateral decompression in elderly patients with degenerative spinal stenosis. For this purpose, we studied the co-morbid condition and clinical outcome of patients who underwent decompressive surgery using the unilateral approach technique. Methods: Thirty-four patients over 65years of age who underwent unilateral partial laminectomy for bilateral decompression from January 2000 to October 2003 were analyzed. These patients were studied for preoperative co-morbid condition and physical status according to the American Society of Anesthesiologists(ASA) classification, postoperative morphometrical change, and clinical outcomes, including visual analogue scale(VAS) score. The mean follow-up was 23months (range 6 - 48months). Results: A patient's physical status was recorded as class I, II, or III by ASA classification, which correlated to 41.2%, 44.1%, and 14.7% of patients, respectively. The cross-sectional area of the pre- and postoperative dural sac at the level of the stenosis was $52.5{\pm}19.9mm^2$ and $110.6{\pm}18.2mm^2$, respectively. The outcome was excellent in 8.8%, good in 58.8%, fair in 23.6%, and poor in 8.8% of the patients. The VAS was changed postoperatively to $3.1{\pm}1.2$. There was no operation-related transfusion yet there was no evidence of postoperative instability at the follow-up examination. Conclusion: Unilateral laminectomy for bilateral decompression, in spite of the limited exposure, can result in satisfactory decompression of the lumbar spinal stenosis and tolerable clinical outcome. This approach is thought to be appropriate for elderly patients who have a greater surgical burden.

• Journal of Acupuncture Research
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• 제15권2호
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• pp.43-60
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• 1998

This study examined the histomorphomeric and histological changes of the left and right kidney in uninephrectomized rat. The results were as follows: 1. In the control, the right kidney was more prominent than the left in the basement membrane of glomerular capillaries. The podocyte had well developed Golgi apparatus in the left kidney and rough endoplasmic reticulum in the right kidney. 2. At the 30 days after unilateral nephrectomy, the basal lamina of glomerular capillaries was prominently thickened in the right kidney. The cytoplasm of the podocyte of the left kidney was markedly increased and had free ribosomes, developed Golgi apparatus and rough endoplasmic reticulum. 3. At the 30 days, the section of the glomeruli were more enlarged in the left kidney than in the right. 4. At the 20 day, the nuclear section of the podocytes were markedly enlarged in the right kidney, but those of the left kidney were diminished. The mitochondrial section of the podocytes were prominently increased in the right kidney. 5. The nuclear section of the parietal layer lining cells was no significant change in the right kidney. That of the left kidney was increased at the 20 days and decreased at the 40 days. The nuclear section of glomerular endothelium of the left kidney increased earlier than the right. 6. In the morphometry of the control kidney, the section areas, long and short diameters, the nuclear section, the mitochondrial section of the proximal tubule cells, and the changes of those were more large in the right kidney than in the left. 7. The luminal secretory vesicles and peroxisomes of the left kidney were more than the right at the 20 days. The increase of mitochodrial section in the proximal tubule cells of the left kidney was more prominent than the right. The large cytoplasmic vacuoles were more prominent in the left kidney than in the right. 8. The thickness of cytoplasm and brush border was more thick in the control left kidney than in the control right. The change of cytoplasmic thickness of the left kidney was increased earlier than in the right and both kineys were increased in the thickness of brush border at the 30 days.

• 한국산학기술학회논문지
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• 제12권2호
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• pp.775-782
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• 2011

가슴샘 비의존성 항원이며 B 림프구의 분열원로 알려져 있는 lipopolysaccharide(LPS)를 발육초기의 C57BL/6계 생쥐에 투여한 후 림프조직의 성숙과정에 따른 면역반응의 양상을 발생학적인 측면에서 형태학적으로 규명하고자 본 실험을 시행하였다. 1. 생후 0일과 3일에 LPS를 주사한 군에서는 주사 후 시간경과에 따라서 림프소절의 총수가 대조군에 비하여 증가되지 않았다. 2. 생후 5일과 7일에 LPS를 주사한 후 2-4주가 경과되었을 때 림프소절의 총수는 대조군에 비하여 유의성있게 증가하였다. 3. 생후 0일에서부터 1주 사이에 LPS를 주사한 후 1-4주가 경과된 실험군에서는 모든 림프절과 심층피질의 면적이 대조군에 비하여 약 1.5-3배정도 커졌다. 4. 생후 3일, 5일 및 1주에 LPS를 주사한 후 2-4주가 경과된 실험군에서는 면적이 0.1 mm2 이상인 림프소절들이 대조군에 비하여 증가하였다. 5. 생후 5일과 1주에 LPS를 주사한 후 2-4주가 경과된 실험군에서는 면적이 0.01 mm2 미만인 림프소절들이 대조군에 비하여 증가하였다. 이상의 실험결과로 미루어 볼 때 LPS의 자극에 의한 새로운 림프소절의 형성능력은 생후 5일-1주에 이루어지며, 또한 새로 생성되는 림프소절들은 면적이 0.01 mm2 미만인 작은 림프소절들일 것으로 예측된다.

• Applied Microscopy
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• 제31권1호
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• pp.59-70
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• 2001

흰쥐를 실험 대상으로 하여 Treadmill 운동을 실시하여 운동에 의한 심근의 적응과 cytochrome oxidase 활성에 관한 세포화학적 연구 알아보고자 실험을 실시하였다. 실험동물은 SD계 웅성 흰쥐를 이용하여 출생 3개월 정도의 체중 $150{\pm}10g$정도로 실험을 시작하였다. 실험군은 일상 대조군과 운동군 2개군으로 나누고, 각각 1일, 3일, 1주일, 4주일, 8주일 및 12주일 동안 각각 4마리씩 운동시켜 실험에 이용하였다. 형태 계측학적 방법으로 심장의 무게변화, 심근섬유와 모세혈관의 비, 그리고 CO 활성정도를 측정하였다. 광학및 전자현미경을 이용하여 관찰한 결과 다음과 같은 결론을 얻었다. 심장무게 변화는 모든 실험군에서 실험기간에 비례하여 증가하였다. 일상 대조군에 비해 운동군에서 유의한 증가를 보였다. 모세혈관과 근섬유 비율은 일상 대조군에서는 유의한 변화가 없었고, 운동군에서는 운동 4주일 이후부터 유의한 증가를 보였다. Cytochrome oxidase C 활성율은 일상 대조군에서는 유의한 변화가 없었고, 운동군에서는 운동 1주일 이후부터 유의한 증가를 보였다. 사립체에 CO활성 형태는 일상 대조군에서는 I형과 III형이, 운동군에서는 실험기간에 비례해서는 II형과 III형이 많이 관찰되었다. 이상과 같은 실험결과들을 미루어볼 때 Treadmill 운동결과 CO의 활성과 심근섬유와 모세혈관의 비율이 운동에 의해 변화를 보여, 흰쥐의 심근에서는 treadmill 운동은 약 4주 정도에서 심장의 적응성이 어느 정도 이루어지는 것으로 사료된다.