• Toxicological Research
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• 제13권1_2호
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• pp.39-48
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• 1997

Cyclophosphamide(25, 50 or 100 mg/kg), orally administered to male Sprague-Dawley rats, caused a time- and dose-dependent thymic atrophy. In the light microscopic examination of the atrophic thymus, thymocytes with condensed or fragmented nucleus were multifocally observed in the cortical region, started to increase 8 hr after CPA treatment and reached to the maximal level at 16 hr, although such cells were not seen after 48 hr when the severe depletion of thymocytes were marked. In agarose gel electrophoresis to analyze the DNA changes, DNA extracted from atrophic thymus showed a oligonucleosomal laddering at the corresponding time to morphological changes. In an additional supportive experiment, thymocytes showing morphological changes, nuclear condensation or apoptotic body, exhibited a positive reaction to immunoperoxidase staining using in situ apoptosis detection kit. Separately, agarose gel electrophoresis of DNA from bone .marrow cells was performed to investigate the involvement of bone marrow cells in the process of thymocyte apoptosis. Although DNA laddering was slightly increased 2 and 4 hr after treatment, no clear correlation was inferred. Taken togather, it is concluded that thymocytes showing morphological changes in thymic atrophy induced by cyclophosphamide administration represent an apoptosis having biochemical nature of programmed cell death.

• 복식
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• 제63권8호
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• pp.125-142
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• 2013

Soek(Xi) was the highest ranked shoe that was worn with the primary formal dress in East Asian countries including ancient Korea and China. This article examined Joseon's Seok as discussed in previous studies, and it explored factors such as the wearers' status, wearing situations, its morphology, its materials, and its colors in the Great Han Empire period (1897~1910), and then extracted three morphological characteristics of the Korean Seok to examine its origin. The results of the study are as follows. For women, the Seok in the Great Han Empire period was worn with Won-sam(圓衫) and No-eui(露衣) as well as Jeok-eui(翟衣), and hence its range of wearing was extensive. Also, red Seok was worn with deep red colored Dae-sam(大衫)-styled Jeok-eui in the Joseon period(1392~1897), and blue Seok with deep blue Jeok-eui in the Great Han Empire period. This suggests the possibility that wearing of deep blue Jeok-eui occurred after 1906 in terms of the use of blue Seok. As for its morphology, its leg-less form was maintained into the late Joseon period, and there were no great changes in its name. The characteristics of the Korean Seok's morphological structure consisted of a shoe leg, the wood-less bottom and pearl ornament. As a result of the examination of the origin of those characteristics, it has been clarified that the form in which Gu, Eok, Jun(純), are attached in the structure with a shoe leg originated from the combination of Hwa(靴) and Li(履) after the two types of shoes were alternately worn in the Song (宋) period. Also, it was confirmed that the woodless bottom appeared between the periods from Wei Jin Northern and Southern Dynasties(魏晉南北朝) to Sui(隋), and the pearl ornament occurred in the Jin(金) period.

• 전자공학회논문지S
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• 제35S권11호
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• pp.106-115
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• 1998

형태론적 영상 분할의 성능은 적절한 마커의 선택에 전적으로 의존한다. 본 논문에서는 경사영상의 국소 최저점으로부터 성장한 담수지역들이 충분한 크기를 가질 때, 마커로 등록하는 마커 배양기를 제안한다. 마커 배양기는 범람 과정이 진행되는 동안, 정의된 마커 영역의 성장, 새로운 마커 영역의 추출, 부적절한 영역에 대한 처리 연기 등을 수행한다. 마커로서의 타당성 진단은 형태론적 필터의 형태소 크기에 의존하는 다중 기준에 의해서 이루어진다. 제안한 마커 배양기는 기존의 방법에 비해서 영역 병합 과정 없이도, 적은 수의 영역으로 동등한 화질을 실현하고, 전송 정보량과 화질 관점에서 우수한 성능을 가지기 때문에, 영역 기반 부호화기를 위한 영상 분할에 적합하다.

• Journal of Ecology and Environment
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• 제40권3호
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• pp.144-152
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• 2016

Background: The purpose of this study is to describe the morphological characteristics of the Aphanizomenon spp. and related species from the natural samples collected in the Nakdong River of South Korea. Results: Morphological characteristics in the four species classified into the genera Aphanizomenon Morren ex Bornet et Flahault 1888 and Cuspidothrix Rajaniemi et al. 2005 were observed by light microscopy. The following four taxa were identified: Aphanizomenon flos-aquae Ralfs ex Bornet et Flahault, Aphanizomenon klebahnii Elenkin ex Pechar, Aphanizomenon skujae $Kom{\acute{a}}rkov{\acute{a}}-Legnerov{\acute{a}}$ et Cronberg, and Cuspidothrix issatschenkoi ($Usa{\check{c}}ev$) Rajaniemi et al. Aph. flos-aquae and Aph. klebahnii always formed in fascicles; the others only occurred in solitary. Aph. flos-aquae was similar to Aph. klebahnii, whereas these species differed from each other by the size and shape of fascicles, which was macroscopic in Aph. flos-aquae and microscopic in the Aph. klebahnii. One of their characteristics was that trichomes are easily disintegrating during microscopic examination. C. issatschenkoi could be clearly distinguished from other species by hair-shaped terminal cell. Its terminal cell was almost hyaline and markedly pointed. Young populations of the species without heterocytes run a risk of a misidentification. Aph. skujae was characterized by akinete. Morphological variability of akinetes from natural samples collected in the Nakdong River was rather smaller than those reported by previous study. Conclusions: C. issatschenkoi are described for the first time in the Nakdong River. In addition, Aph. klebahnii and Aph. skujae are new to South Korea.

• 대한수의학회지
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• 제40권4호
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• pp.769-775
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• 2000

This study was investigated the ovarian response following superovulation treatment at different day of diestrus. The criterion for the presence or absence of a dominant follicle based on their morphological examination. Dominant follicle was puntured 48 hrs before the oneset of superovulation treatment by ultrasonography guided aspiration needle. Superovulation was induced by subcutaneous administration of FSH twice a day for 4 day in a decreasing regimen. There was no significant different between presence of dominont follicle and progesterone concentration/diameter of corpus luteum in HanWoo. Number of corpus luteum of donor after superovulation treatment was not significantly different in FSH administration at day 9, 11 and day 13 of estrus($14.5{\pm}4.5$, $15.5{\pm}5.6$ and $11.0{\pm}5.5$, respectively). But, the diameter of CL was significantly correlate(R2 = 0.757) with progesterone levels on day of superovulatory induction. After 7 days of artificial insemination, the embryos at 7 days were collected by uterine flushing after dominant follicle aspiration and superovulation treatment, and evaluated their quality by morphological criteria. Fifty five embryos with excellent, good and fair grade were transferred into 24 recipient cows. Seventeen offsprings, 1 of triplet, 4 of twins and 6 of singlet, were yield from 10 recipient cow. In conclusion, the present study showed that 1) dominant follicle can be determined by ultrasonography with rectal palpation by morphological evaluations, 2) superovulation response after follicular aspiration was not differ at day 9, 11 and 13 of estrus, 3) dominant follicle did not affect to progesterone concentration and diameter of CL, and 4) diameter of CL was significantly correlate to the level of progesterone concentrations in HanWoo.

• 동의생리병리학회지
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• 제26권5호
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• pp.738-744
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• 2012

Gastrodia elata Blume is used for a variety of purposes including treatment of inflammation in the Korean medicine. The present study investigated whether the G. elata extracts have the anti-inflammatory effect on lipopolysaccharide(LPS)-stimulated BV-2 microglia cells. G. elata extracts showed an anti-inflammatory effects in the morphological and nitric oxide(NO) analysis, especially in hexane extract. So we investigated the hexane extract from G. elata in the following experiments. Hexane extract significantly inhibited the secretion of NO with protein level of inducible nitric oxide synthase in LPS-stimulated BV2 microglia cells. Hexane extract also inhibited LPS-stimulated inflammatory responses involving the degradation of cytosolic inhibitory(I)-${\kappa}B{\alpha}$ and the translocation of nuclear factor(NF)-${\kappa}Bp65$ to nucleus in LPS-stimulated BV-2 microglia cells by morphological analysis. Western blot analyse confirmed that I-${\kappa}B{\alpha}$ and NF-${\kappa}Bp65$ showed a similar pattern as morphological analysis. Our results suggest that G. elata extracts, especially hexane extract, may act as a therapeutic agent for inflammatory disease in the central nervous system through a selective regulation of NO production and NF-${\kappa}B$ activation.

• 식물분류학회지
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• 제51권2호
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• pp.133-140
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• 2021

북아메리카 원산이며, 일본에서는 외래식물로 알려진 미국잔디갈고리(Desmodium paniculatum)가 국내의 화성시와 대전시에서 발견되었다. 본종은 다년생 초본이며, 협과는 4-5개의 소절과로 연결되고, 소절과 사이의 배축면이 완만하게 좁아지는 점에서 국내의 근연분류군들과 구분된다. 미국잔디갈고리는 강변과 도로변에서 자라고 있어, 하천정비나 도로 공사를 통해 유입된 것으로 추측된다. Internal transcribed spacer 계통수상에서 한국산 미국잔디갈고리는 일본산 개체와 단계통을 형성하였다. 본 연구는 미국잔디갈고리의 형태적 특징에 대한 기재와 사진, 도해, 분포 위치에 대해 보고하였다.

• Archives of Plastic Surgery
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• 제50권6호
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• pp.615-620
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• 2023

Massive localized lymphedema (MLL) is a rare disease caused by the obstruction of lymphatic vessels with specific clinical morphological and radiological characteristics. People with morbid obesity are mainly affected by MLL. Lymphedema is easily confused with soft tissue sarcoma and requires differential diagnosis, both the possibility of an MLL and also carcinoma manifestations in the soft tissues. The possible causes of massive lymphedema include trauma, surgery, and hypothyroidism. This report is the first case of MLL treated surgically in the Russian Federation. Detailed computed tomography (CT) characteristics and an electron microscope picture of MLL are discussed. A 50-year-old woman (body mass index of 43 kg/m²) with MLL arising from the anterior abdominal wall was admitted to the hospital for surgical treatment. Its mass was 22.16 kg. A morphological study of the resected mass confirmed the diagnosis of MLL. We review etiology, clinical presentation, diagnosis, and treatment of MLL. We also performed an electron-microscopic study that revealed interstitial Cajal-like cells telocytes not previously described in MLL cases. We did not find similar findings in the literature. It is possible that the conduction of an ultrastructural examination of MLL tissue samples will further contribute to the understanding of MLL pathogenesis.

• 대한임상검사과학회지
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• 제49권4호
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• pp.359-365
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• 2017

요침사 검사는 물리화학적 검사보다 정도관리의 표준화가 어려운 실정이다. 본 연구에서는 요침사 정도관리의 합리성을 위해 요침사 보존제인 글루타르알데히드로 요침사를 보존하여 실물을 통한 현미경 검사로 요침사 정도관리가 가능한지를 알아보고자 하였다. 2.5% 글루타르알데히드를 보존액으로 하여 표본을 제작하였다. 보존제를 처리한 검체는 1주일 간격으로 4주간 세포의 형태학적 변형을 확인하였고, 이후 4주간은 보존여부를 확인하였다. 요침사 슬라이드 제작 후 2개의 슬라이드를 제작하였으며, 하나는 냉장보관, 다른 하나는 실온 보관하여 검체의 형태학적 변형을 확인하였다. 글루타르알데히드는 요침사를 냉장 보관하여 8주까지 보존하는 효과가 있었으며, 슬라이드 제작 후에는 냉장 보관하여 3일까지 안정한 것으로 나타났다. 또한 보존제 처리 후 슬라이드 제작하여 검사실간, 검사자간판독을 비교한 결과 낮은 일치도를 보였다. 결론적으로 본 연구를 통해 요침사 성분을 보존할 수 있으며, 실물을 통한 정도관리 및 교육용으로 활용될 것으로 기대된다.

• 동의생리병리학회지
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• 제17권1호
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• pp.190-202
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• 2003

The purpose of this study is to examine the effects of Gamimahaenggamseok-tang and Typhae Pollen on Body Weight and Lipid Metabolism of Rats with Obesity induced by high fat diet. Sixty four male Sprague-Dawley rats, around 250g of B.W., were divided into normal, control, sample A and sample B groups. Normal groups were orally administrated with saline and normal animal feed, and control groups with saline and high fat diet, sample A with liquid extract of Gamimahaenggamseok-tang and high fat diet, and sample B with liquid extract of Typhae Pollen and high fat diet. After 4 and 8 weeks administration, body weight, epididymal fat pad weight, weight of liverㆍspleenㆍkidney, abdominal skin fold thickness, feed intake, and lipid level in liver tissue on morphological examination; total cholesterol, HDL-cholesterol, LDL-cholesterol, triglyceride, free fatty acid, and phospholipid on serum examination were measured in all of the groups.