• Title/Summary/Keyword: Morphological development

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Brown Rot of Apricot and Mume Caused by Phomopsis vexans

  • Kim, Wan-Gyu;Hong, Sung-Kee;Cho, Weon-Dae;You, Chang-Hyun
    • The Plant Pathology Journal
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    • v.19 no.5
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    • pp.231-234
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    • 2003
  • Brown rot symptoms severely occurred on fruits of apricot and mume grown in Changnyeong, Suwon and Yeongi in Korea during a disease survey in June 2002. A total of 32 isolates of Phomopsis sp. was obtained from the fruit rot symptoms. All isolates were identified as Phomopsis vexans based on their morphological and cultural characteristics. Four isolates of the fungus were tested for pathogenicity to fruits of apricot and mume by artificial inoculation. All isolates induced brown rot symptoms on the fruits of apricot and mume by wound inoculation. Unwounded inoculation also induced symptoms on mume fruits but not on apricot fruits. The symptoms on the fruits induced by artificial inoculation were similar to those observed in the orchard. The pathogenicity tests revealed that mume was more susceptible to the pathogen than apricot. This is the first report of brown rot of apricot and mume caused by P. vexans.

New Record of the Black-Velvet Angelfish, Chaetodontoplus melanosoma (Pomacanthidae, Perciformes) from Korea (한국 제주도에서 채집된 청줄돔과 어류 1미기록종, Chaetodontoplus melanosoma)

  • Han, Song Hun;Kim, Maeng Jin;Song, Choon Bok
    • Korean Journal of Ichthyology
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    • v.27 no.3
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    • pp.249-252
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    • 2015
  • This is the first report of Chaetodontoplus melanosoma, belonging to the family Pomacanthidae, collected in Korea. The specimen (123.1 mm in standard length) was caught in the coastal waters of Jejudo Island by a trammel net on 23 February 2010. This species was characterized by the following morphological traits: 26 dorsal fin rays, 16 pectoral fin rays, 6 pelvic fin, 21 anal fin rays, no blue strips on the body, no markings behind the eye, and an egg-shaped faint black spot on the base of the pectoral fin. We suggest a new Korean name "Geom-eun-cheong-jul-dom" for C. melanosoma.

AN EXPERIMENTAL STUDY ON EFFECT OF RADIATION IN PALATE DEVELOPMENT OF RAT EMBRYO (방사선조사시 태내백서의 구개형성기에 미치는 영향에 관한 실험적 연구)

  • Khim Jhai Dhuck
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.6 no.1
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    • pp.45-50
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    • 1976
  • The author observed morphological change in palate development of rat embryo after irradiation of x-ray on the one side of the duplex uterus. The time-matings occured between 6 p.m. and 8 p.m. and all females with copulation plugs at 8 a.m. were isolated and properly marked for evidence of copulation. The lower left abdomen of mothers were exposed to x-radiation on the 7 1/2th, 9 1/2th, 11 1/2th day of gestation, respectively 150, 250, 350, 500rads. At 18 1/2th day of post-conception, the pregnant females were dissected and the contents of the two uteri examined. The translucent sample by Alizarin red S stain were prepared. The results were as follows; 1. The result that groups irradiated by 250rads and 350rads made marked difference in comparison with the control group suggests the x-ray to be a inducing factor of cleft palate. 2. At 11 1/2th day of gestation, incidence of cleft palate induced by x-irradiation was highest. 3. Mortality showed the highest frequency at 7 1/2th day of gestation and tended to decrease in according to increasing of age. 4. Morphology of cleft palate induced by x-irradiation showed similarity in comparison with those induced by other factors having reported ever.

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Morphological Characteristics of Conidiogenesis in Cordyceps militaris

  • Shrestha, Bhushan;Han, Sang-Kuk;Yoon, Kwon-Sang;Sung, Jae-Mo
    • Mycobiology
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    • v.33 no.2
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    • pp.69-76
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    • 2005
  • Conidial development of Cordyceps militaris was observed from germinating ascospores and vegetative hyphae through light and scanning electron microscopy (SEM). Ascospores were discharged from fresh specimens of C. militaris in sterile water as well as Sabouraud Dextrose agar plus Yeast Extract (SDAY) plates. We observed ascospore germination and conidial formation periodically. Under submerged condition in sterile water, most part-spores germinated unidirectionally and conidia were developed directly from the tips of germinating hyphae of part-spores within 36 h after ascospore discharge, showing microcyclic conidiation. First-formed conidia were cylindrical or clavate followed by globose and ellipsoidal ones. Germination of ascospores and conidial development were observed on SDAY agar by SEM. Slimy heads of conidia on variously arranged phialides, from solitary to whorl, developed 5 days after ascospore discharge. Besides, two distinct types of conidia, elongated pyriform or cylindrical and globose, were observed in the same slimy heads by SEM. Conidia were shown to be uninucleate with 4,6-diamidino-2-phenylindole staining. Conidiogenous cells were more slender than vegetative hyphae, having attenuated tips. Microcyclic conidiation, undifferentiated conidiogenous hyphae (phialides), polymorphic conidia and solitary, opposite to whorled type of phialidic arrangement are reported here as the characteristic features of asexual stage of C. militaris, which can be distinguished from other Cordyceps species.

Characterization of a Unique New Strain Named the NFRDI N°1 Rotifer Strain, a Brackish Brachionus Rotifer Collected from a South Korea Coastal Lagoon

  • Jung, Min-Min
    • Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.333-337
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    • 2011
  • A new and a unique Brachionus rotifer was found in Hwajinpo coastal lagoon in Gangwon Province, South Korea. This Brachionus certainly originated from the wild rather than from aquaculture stations because Hwajinpo coastal lagoon has been under rigorous control as a military protected area and therefore could not have been contaminated by aquaculture stations. The new strain was identified as Brachionus rotundiformis based upon its morphological characteristics. The parthenogenetic female of this new rotifer strain typically shows characters similar to those of B. rotundiformis, such as the pot shape of the body, rounded dorsal plate compared with flattened ventral plate, elliptical mictic egg, four frontal spines, six pointed occipital spines, non-nodal foot, two toes, trophi typical of the Brachionus genus with five uncus plates resembling comb teeth, one wide symmetrical manubrium and ramus, and no stiffened spine as is seen in freshwater Brachionus rotifers. Moreover, its lorica was rather small in size compared with other common rotifer strains that serve as live-food organisms (Guam, Thai, and Bali strains). This new and unique Korean brackish rotifer, a B. rotundiformis strain, was therefore named the National Fisheries Research and Development Institute (NFRDI) $N^{\circ}1$ rotifer strain.

Occurrence of Dry Rot on Cymbidium Orchids Caused by Fusarium spry. in Korea

  • Kim, Wan-Gyu;Lee, Byung-Dae;Cho, Weong-Dae;Sung, Jae-Mo
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.156-160
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    • 2002
  • Cymbidium orchids with blight and rot symptoms were collected, and a total of 63 isolates of Fusarium app. was obtained from pseudobulbs, roots, and leaves of the diseased plants. The isolates were identified based on their morphological characteristics. Out of the 63 isolates of Fusatium sup., 51 isolates were identified as F. oxysporum, 10 isolates as F. solani, and the rest as F. proliferatum. F. oxysporum was isolated from all the Cymbidium spp., while F. solani and F. proliferatum were isolated only from Cymbidium ensifolium and C. ginatum, respectively. Isolates of the three Fusarium spp. were tested for pathogenicity to their hosts by artificial inoculation. The strongly pathogenic isolates of Fusarium spp. induced severe dry rot of pseudobulbs and roots of the host plants. The symptoms progressed up to the basal part of the leaves, which later caused blight of the entire plant. The dry root symptoms induced on the plants by artificial inoculation with the isolates of Fusarium app. were similar to those observed in the growers'greenhouses. This is the first report of dry rot of Cymbidium spp. caused by F. oxysporum, F. solani, and F. proliferatum in Korea.

A Morphological and Histopatholocial Study on Dactylogyrus sp. of Parasitizing of Cultured Sea bass, Lateolabrax japonicus (양식(養殖)농어에 기생(寄生)한 닥티로지러스충(蟲)의 형태학적(形態學的) 및 병리조직학적(病理組織學的) 연구(硏究))

  • Sim, Doo-Saing;Jung, Sung-Hee;Chun, She-Kyu;Park, Hyung-Sook
    • Journal of fish pathology
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    • v.2 no.2
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    • pp.75-82
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    • 1989
  • Dactylogyrosis due to Dactylogyrus sp. occured among cultured sea bass (Lateolabrax japonicus) in Geoje, Kyoung Nam Prefecture in July, 1988. Descriptions are given of the opisthohaptor, copulatory organ and also of the structures in Dactylogyrus sp.. Dactylogyrus sp. have one pair of minute anchor (length : $15-22{\mu}m$) and 14 larger hooks. Histopathological changes of the heavily infested gills are showed necrosis, epithelium of the gill filaments underwent hyperplasia with fusion of the lamellae and filamental clubbing. And a bacterial colony is invaded on the surface of lamellar epithelium.

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Effect of EDTA on the In Vitro Development of Parthenogenetic Mouse Eggs (EDTA가 생쥐 단위발생란의 체외 발달에 미치는 영향)

  • 곽대오;김선구;김영수;박충생
    • Korean Journal of Animal Reproduction
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    • v.17 no.4
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    • pp.365-373
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    • 1994
  • To investigate the effect of EDTA on the in vitro development of parthenogenetic eggs of ICR strain mice, those were cultured in 35mm culture dishes containing NaHCO3-BMOC-3 medium supplemented with 10, 50, 100, or 500$\mu$M of EDTA at 37$^{\circ}C$ for 96hrs. under the atmosphere of 5% CO2 and 95% air. EDTA supplementation of 10, 50, or 100$\mu$M to medium significantly(P<0.01) increase morula and blastocyst formation rate compared with controls in haploid(19.8, 25.9, 39.0% vs. 0.0%). And compared with 10, or 50$\mu$M of EDTA supplementation, significantly(P<0.01) higher morula and blastocyst formation rate resulted from EDTA supplementatin of 100$\mu$M. Both the nuclear number and diameter of blastocysts developed from parthenogenetic eggs were not affected by the morphological types when they were cultured, or the supplementary concentrations of EDTA. The nuclear number of blastocysts developed from haploid, diploid, and immediately cleavaged eggs was 44.8$\pm$1.2, 45.2$\pm$1.5, and 45.4$\pm$1.8, respectively. And the diameter of those eggs ranged 104.4$\pm$1.8, 104.3$\pm$1.2, and 103.8 1.3$\mu$m, respectively.

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Comparative Analysis of Root and Shoot Growth between Tongil and Japonica Type Rice

  • Kang, Si-Yong;Shigenori Morita
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.3
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    • pp.161-167
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    • 1998
  • Root and shoot development of two rice (Oryza sativa L.) cultivars with different genetic backgrounds was studied with reference to their relative growth. Tongil type (indica-japonica hybrid) cultivar 'Kuemkangbyeo' and japonica cultivar 'Koshihikari' were grown in $5000^{-1}$ a Wagnar pots under flooded condition. Three plants with roots of both cultivars were taken in every phyllochron through the heading stage to record morphological characteristics of shoot and root system. Compared to Koshihikari, Kuemkangbyeo produced more tillers and had greater shoot weight and leaf area per hill. Length and weight of the root system in both cultivars increased exponentially with time. At the same time, root system development was significantly faster in Kuemkangbyeo than in Koshihikari after the panicle initiation stage. As a result, Kuemkangbyeo has a vigorous root system which consists of larger number of nodal roots compared to Koshihikari. Also, the root length and weight per unit leaf area of Kuemkangbyeo were larger than those of Koshihikari in the later half of growing period, which suggests possible higher physiological activity of the root system of Kuemkangbyeo which is known as a high-yielding cultivar. The relationship between root traits (crown root number, total root length, and root dry weight) and shoot traits (leaf area and leaf+culm dry weight) in both cultivars closely showed allometry until the flag leaf stage.

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Four Newly Recorded Amanita Species in Korea: Amanita sect. Amanita and sect. Vaginatae

  • Kim, Chang Sun;Jo, Jong Won;Kwag, Young-Nam;Oh, Junsang;Shrestha, Bhushan;Sung, Gi-Ho;Han, Sang-Kuk
    • Mycobiology
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    • v.41 no.3
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    • pp.131-138
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    • 2013
  • We collected nearly 70 specimens of Amanita species during a diversity study of Korean mushrooms conducted in 2012. In this study, we primarily investigated 23 Amanita specimens belonging to sections Amanita and Vaginatae. Based on sequence data of the internal transcribed spacers and partial large subunit of ribosomal RNA and morphological characteristics, we identified the following 15 phylogenetic species: A. alboflavescens, A. ceciliae, A. farinosa, A. fulva, A. griseofolia, A. ibotengutake, A. melleiceps, A. orientifulva, A. pantherina, A. rubrovolvata, A. sinensis, A. subglobosa, A. vaginata, A. cf. vaginata f. alba, and an undescribed Amanita species. In this study, four of the identified Amanita species (A. griseofolia, A. ibotengutake, A. orientifulva, and A. sinensis) were reported for the first time in Korea.