• 한국지리정보학회지
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• 제20권1호
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• pp.152-161
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• 2017

모바일 매핑 시스템(Mobile Mapping System: MMS) 센서를 이용한 3차원 정밀지도의 구축은 자율주행 자동차 개발에 필요한 기술이다. 본 논문은 MMS LiDAR(Light Detection And Ranging) 센서를 이용하여 획득한 점군자료를 이용하여 도로주변의 3차원 객체 추출에 관한 연구를 수행하였다. 우선, MMS LiDAR 점군자료 이용하여 수치표면모형(DSM: Digital Surface Model)을 제작하고, 생성된 DSM을 기반으로 경사도 지도를 제작하였다. 추출된 경사도 정보를 이용하여 도로주변의 3차원 객체를 식별하였고, 모폴로지 필터링 기법을 이용하여 도로주변의 3차원 객체 중 총 97%의 객체를 추출하였다. 본 연구는 MMS 센서를 이용하여 획득한 공간정보자료를 기반으로 도로 주변의 3차원 객체를 추출함으로써 최근 주목받고 있는 자율주행기술의 활용성에 관한 방안을 제시하였다는데 의의가 있다.

• 한국정보통신학회논문지
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• 제7권5호
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• pp.1052-1061
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• 2003

본 논문은 SOM 알고리즘을 이용한 차량 번호판 인식 방법을 제안하고 차량 번호판 인식을 이용한 주차관리 시스템 개발에 대해서 기술한다. 차량 영상에서 번호판 영역을 추출하기 위해 수평$.$수직 에지의 형태학적 정보를 이용하고, 추출된 번호판에서 문자를 포함하는 특징 영역을 추출하기 위해 4 방향 윤곽선 추적 알고리즘을 이용한다. 추출된 특징 영역의 인식은 SOM 알고리즘을 적용한다. 50개의 실제 차량 영상을 실험한 결과, 제안된 번호판 영역 추출 방법이 기존의 RGB 정보를 이용한 방법과 HSI를 이용한 방법보다 추출율이 개선되었다. 그리고 SOM 알고리즘을 이용한 차량 번호판 인식이 효율적인 것을 확인하였다. 실험을 통하여 성능 향상을 보인 제안된 차량 번호판 인식 방법을 이용하여 주차 관리 시스템을 개발하였다.

• Clinical and Experimental Reproductive Medicine
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• 제42권4호
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• pp.156-162
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• 2015

Objective: To investigate fertilization and embryo quality of dysmorphic mature oocytes with specific morphological abnormalities obtained from intracytoplasmic sperm injection (ICSI). Methods: The fertilization rate (FR) and embryo quality were compared among 58 dysmorphic and 42 normal form oocytes (control 1) obtained from 35 consecutive ICSI cycles, each of which yielded at least one dysmorphic mature oocyte, performed over a period of 5 years. The FR and embryo quality of 441 normal form oocytes from another 119 ICSI cycles that did not involve dysmorphic oocytes served as control 2. Dysmorphic oocytes were classified as having a dark cytoplasm, cytoplasmic granularity, cytoplasmic vacuoles, refractile bodies in the cytoplasm, smooth endoplasmic reticulum in the cytoplasm, an oval shape, an abnormal zona pellucida, a large perivitelline space, debris in the perivitelline space, or an abnormal polar body (PB). Results: The overall FR was significantly lower in dysmorphic oocytes than in normal form oocytes in both the control 1 and control 2 groups. However, embryo quality in the dysmorphic oocyte group and the normal form oocyte groups at day 3 was similar. The FR and embryo quality were similar in the oocyte groups with a single abnormality and multiple abnormalities. Specific abnormalities related with a higher percentage of top-quality embryos were dark cytoplasm (66.7%), abnormal PB (50%), and cytoplasmic vacuoles (25%). Conclusion: The fertilization potential of dysmorphic oocytes in our study was lower, but their subsequent embryonic development and embryo quality was relatively good. We were able to define several specific abnormalities related with good or poor embryo quality.

• 한국컴퓨터정보학회논문지
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• 제17권10호
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• pp.25-33
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• 2012

본 논문에서는 모바일 환경에서 음성인식한 결과에 포함된 오류를 교정하는 규칙기반 접근방법을 제안한다. 제안하는 방법은 처리시간이나 메모리에 제약을 받는 모바일 환경을 고려하여 다음과 같이 구성된다. 오류 교정 속도를 최소화하기 위해서, 음절 해체 및 조합 과정이나 형태소 분석 등의 처리를 줄이고, 최장일치 규칙 선택기준을 바탕으로 오류 발생 추정 지점에서 교정 후보도 하나만 생성한다. 제안하는 방법은 메모리를 효율적으로 사용하기 위해서, 어절사전이나 형태소분석기를 사용하지 않고, 규칙도 유형별로 따로 구분하지 않고 통합하여 저장한다. 제안하는 방법은 모델의 수정 및 유지보수가 용이하도록, 오류교정규칙을 학습말뭉치에서 자동으로 추출하여 구축한다. 실험결과 제안하는 방법은 음성인식 결과에 대하여 정확률을 5.27% 정도 재현율을 5.60% 정도 개선하였다.

• 식물분류학회지
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• 제32권4호
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• pp.397-416
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• 2002

한국산 제비꽃속 노랑제비꽃절 (Viola section Chamaemelanium) 식물에 대해 광학현미경 및 전자현미경을 사용하여 형태 분류학적 연구를 수행하였다. 지하경과 지상경의 특징, 자방 돌기 그리고 주두의 모양과 돌기 등의 특징에 의해 V. orientalis와 V. brevistipulata가 구별되었다. 나아가 후자는 잎의 특징에 따라 3 변종 즉 var. brevistipulata, var. minor 그리고 var. laciniata 등으로 세분되었다. Viola brevistipulata var. laciniata는 거치 수가 가장 많고, 불규칙한 치상의 복거치를 갖는다. 그러나 다른 두 변종은 규칙적인 파상 또는 치상의 거치를 갖는다. 세 변종간 잎의 크기는 V. brevistipulata var. brevistipulata가 가장 크고 V. brevistipulata var. minor가 가장 작다. 이들 중 V. brevistipulata var. laciniata는 한국 미기록 종이다. 또한 자방과 주두에 발달된 돌기물의 모양 및 배열양상은 본 절내 종을 동정할 때 중요한 표징형질로 확인되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.130-130
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• 2017

Sorghum bicolor is considered as copper-tolerant species. The present study was conducted to understand the copper tolerance mechanism in Sorghum seedling roots. Morphological and effects of Cu on other interacting ions were observed prominently in the roots when the plants were subjected to different concentrations (0, 50, and $100{\mu}M$) of $CuSO_4$. However, the morphological characteristics were reduced by Cu stress, and the most significant growth inhibition was observed in plants treated with the highest concentration of $Cu^{2+}$ ions ($100{\mu}M$). In the proteome analysis, high-throughput two-dimensional polyacrylamide gel electrophoresis coupled with MALDI-TOF-TOF mass spectrometry was performed to explore the molecular responses of Cu-induced sorghum seedling roots. In two-dimensional silver-stained gels, a total of 422 differentially expressed proteins (${\geq}1.5-fold$) were identified using Progenesis SameSpot software. A total of 21 protein spots (${\geq}1.5-fold$) from Cu-induced sorghum roots were analyzed by mass spectrometry. Of the 21 differentially expressed protein spots from Cu-induced sorghum roots, a total of 10 proteins were up-regulated, and 11 proteins were down-regulated. The abundance of the most identified protein species from the roots that function in stress response and metabolism was significantly enhanced, while protein species involved in transcription and regulation were severely reduced. The results obtained from the present study may provide insights into the tolerance mechanism of seedling roots in Sorghum.

• 한국치위생학회지
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• 제15권4호
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• pp.719-725
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• 2015

Objectives: The purpose of this study was to determine the toxic effects of sodium lauryl sulfate(SLS) in human keratinocyte HaCaT cells and mouse fibroblast NIH-3T3 cells. Methods: The effect of sodium lauryl sulfate(SLS) cell viability and proliferation were determined by WST-1 assay and changes shape of nucleus were evaluated by Hoechst staining under fluorescence microscopy. Additionally, observation of cell morphological changes under light microscopy. Results: SLS induced cytotoxicity and a marked apoptosis in both HaCaT and NIH-3T3 cell lines. With the result of the WST-1 assay, SLS induced the cytotoxicity of 0.005% and 0.0075%, 0.01% SLS for 24 h after HaCaT and NIH-3T3 cells in time and dose-dependent manner(p<0.005). SLS inhibited cell growth and caused apoptosis as evidenced by nuclear fragmentation and condensation. Thus, determination of the morphological changes to define apoptosis was visualized using inverted phase contrast microscopy. Conclusions: SLS had toxicity of the human keratinocyte cells and mouse fibroblast cells and this study will provide the basic data for the development of proper SLS concentration in dentifrice.

• 원예과학기술지
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• 제32권4호
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• pp.550-557
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• 2014

Heloniopsis koreana and Heloniopsis tubiflora (Melanthiaceae) are endemic herbaceous species of the Korean Peninsula. The Melanthiaceae family has been described as having seeds with small, underdeveloped embryos at the time of dispersal, and morphological (MD) or morphophysiological dormancy (MPD). However, there are few reports on embryo growth, morphology, and seed germination in Heloniopsis species. The aims of this study were to investigate embryo growth and seed dormancy, and to determine the type of dormancy exhibited by these species. The effects of incubation temperatures, light conditions, and gibberellic acid ($GA_3$) on dormancy break and seed germination were tested. Freshly matured seeds of the two species had small embryos that occupied about 9-11% of the length of the endosperm, and which increased by more than 300% in length before radicle emergence, indicating that the embryos are underdeveloped at the time of dispersal. Embryos in the seeds grew under warm temperature regimes (between $25/15^{\circ}C$ and $30/20^{\circ}C$). $GA_3$ application (tested only in the light) overcame seed dormancy and promoted germination. Approximately 30% of the seeds of H. koreana and approximately 40% of the seeds of H. tubiflora germinated in suitable environmental conditions (light and temperature) within 4 weeks. Therefore, 30-40% of the seeds of the two species exhibited MD, and the rest of the seeds had non-deep simple MPD. Light was found to be one of the critical factors for germination because no seed of either of the two Heloniopsis species germinated under constant dark conditions, and thus, these species have the potential to form a persistent soil seed bank. Understanding these germination requirements will help in development of effective strategies to increase the establishment of seedlings in their native habitat.

• Journal of Forest and Environmental Science
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• 제11권1호
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• pp.61-71
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• 1995

한국산(韓國産) 쪽버들의 정확한 실체를 파악하기 위하여 형태학적(形態學的), 화분학적(花粉學的)으로 재검토하였으며, 어떠한 일정한 지역에만 분포(分布)하고 있는 쪽버들의 격리분포(隔離分布) 특성에 대한 조사를 행하였다. 1. 쪽버들의 외부형태학적(外部形態學的)인 특성(特性)을 그림과 함께 명확히 하였다. 2. 쪽버들의 화분학적(花粉學的) 특성(特性)은 하나의 명확한 종(種)임을 입증하였다. 3. 쪽버들은 강원도(江原道) 설악산의 백담계곡 일부 지역과 한계령의 양쪽 계곡 지역에 격리분포(隔離分布)하고 있으며, 북한의 성북(成北) 경성군(鏡城郡)과 성남(成南) 신흥군(新興郡) 및 풍산군豊山郡)의 일부 고지대(高地帶)의 계곡 지역에만 분포하고 있다. 4. 쪽버들의 격리분포(隔離分布)에 영향을 미치는 환경인자(環境因子)로는 수환경(水環境)의 지배가 큰 것으로 추정되었다. 5. 쪽버들의 생태학적(生態學的), 분류학적(分類學的) 정보들은 사방수종(砂防樹種), 목재생산(木材生産), 귀지공간조성(歸地空間造成) 등과 같은 조림(造林)에 관한 실제적인 적용에 이용될 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.3191-3196
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• 2013

Leaf extracts of Cassia alata L (akapulko), traditionally used for treatment of a variety of diseases, were evaluated for their potential antitumor properties in vitro. MTT assays were used to examine the cytotoxic effects of crude extracts on five human cancer cell lines, namely MCF-7, derived from a breast carcinoma, SK-BR-3, another breast carcinoma, T24 a bladder carcinoma, Col 2, a colorectal carcinoma, and A549, a nonsmall cell lung adenocarcinoma. Hexane extracts showed remarkable cytotoxicity against MCF-7, T24, and Col 2 in a dose-dependent manner. This observation was confirmed by morphological investigation using light microscopy. Further bioassay-directed fractionation of the cytotoxic extract led to the isolation of a TLC-pure isolate labeled as f6l. Isolate f6l was further evaluated using MTT assay and morphological and biochemical investigations, which likewise showed selectivity to MCF-7, T24, and Col 2 cells with $IC_{50}$ values of 16, 17, and 17 ${\mu}g/ml$, respectively. Isolate f6l, however, showed no cytotoxicity towards the non-cancer Chinese hamster ovarian cell line (CHO-AA8). Cytochemical investigation using DAPI staining and biochemical investigation using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-a method used to detect DNA fragmentation-together with caspase assay, demonstrated apoptotic cell death. Spectral characterization of isolate f6l revealed that it contained polyunsaturated fatty acid esters. Considering the cytotoxicity profile and its mode of action, f6l might represent a new promising compound with potential for development as an anticancer drug with low or no toxicity to non-cancer cells used in this study.