• Title/Summary/Keyword: Morphological changes

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Effect of Prostaglandin $F_2\alpha$ Administration on the Luteal Cell of Korean Native Cattle (Prostaglandin $F_2\alpha$의 투여가 한우황체의 조직상에 미치는 영향)

  • 최병상;박민근;정길생
    • Korean Journal of Animal Reproduction
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    • v.4 no.1
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    • pp.13-19
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    • 1980
  • This experiment was carried out to observe early morphological changes of luteal cells in Korean native cattle treated with prostaglandin F2$\alpha$. Twenty five gram of prostaglandin F2$\alpha$ was administrated per cow at 10 days after ovulation and luteal cells were removed 30, 60, 120 and 180 minites after administration. Morphological changes of each luteal cell was observed by electron microscope. the results obtained were summarized as followings: 1. Many electron-dense granules were observed in luteal cells obtained from control cow but those granules were decreased rapidly after 30 minutes of administration and no granules were obresved after 180 minutes of administration. 2. In control, the shape of mitochondria were begining to collapse from the time of 60 minutes after administration. After 180 minutes of administration, mitochondria were swelled extreamly. 3. Lipid droplets in luteal cell were increased in its size and number with the duration of time after administration. 4. Shape of smooth endoplasmic recticulum was vesicular and its dimension and number were increased according to time course after administration.

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Pathogenic and Morphologic Variation of Toxoplasma gondii by X-Ray and Neutron Flax Irradiation (X-Ray 및 Neutron 조사에 의한 Toxoplasma gondii의 병원성 및 형태학적 변화에 대하여)

  • Han, T.W.
    • Korean Journal of Veterinary Research
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    • v.10 no.2
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    • pp.21-51
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    • 1970
  • Series of experiments were conducted to determine lethal does of X-ray and Neutron on Toxoplasma gondii. strain RH and IRI. As well morphological changes of Toxoplasma gondii irradiated or not were compared by use of electron microscope. The pathogenicity test of the irradiated and nonirradiated Toxoplasma gondii was made in mice guinea-pigs, rabbits and pigs: The letahl dose of X-ray and Neutron on RH and IRI strain and the growth rate between two strains after irradiation were shown little differences. Morphological changes were not observed until 18th passage was made. After then, the growth rate was decreased apparently, and atrophied forms were frequently observed in electron microscope. Survival time of animals inoculated with irradiated strain was longer than that of animals giving non-irradiated strain, and Toxoplasma gondii were isolated from all the dead animals. But it is of interest that pigs survived after injection of Toxoplasma gondii remained health and much attempts were failed toisolate Toxplasma gondii remained health and much attempts were slaughtered them. Animals were succumbed after injection of Toxoplasma gondii without any relationship with serum titers. (HA antibody).

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Morphological and functional changes of dissociated single pancreatic acinar cells: testing the single cell as a model for exocytosis and calcium signaling

  • Lee, Misun;Uhm, Dae-Yong;Park, Myoung-Kyu
    • Proceedings of the Korean Biophysical Society Conference
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    • 2003.06a
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    • pp.56-56
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    • 2003
  • Isolated single pancreatic acinar cells have long been used as a good model for studying many kinds of signaling processes due to their good structural and functional polarities without a significant validation. In this study, we have examined morphological and functional changes of the dissociated single pancreatic acinar cells by imaging cytosolic Ca$\^$2+/ concentration, exocytosis of granules, and by observing their shapes with confocal microscopy.

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Effect of Sub-Minimal Inhibitory Concentration Antibiotics on Morphology of Periodontal Pathogens

  • Kwon, Ye Won;Lee, Si Young
    • International Journal of Oral Biology
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    • v.39 no.2
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    • pp.115-120
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    • 2014
  • Minimal inhibitory concentration (MIC) is the lowest concentration of antibiotics that inhibits the visible growth of a microorganism. It has been reported that sub-MIC of antibiotics may result in morphological alterations along with biochemical and physiological changes in bacteria. The purpose of this study was to examine morphological changes of periodontal pathogens after treatment with sub-MIC antibiotics. Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Porphyromonas gingivalis were used in this study. The MIC for amoxicillin, doxycycline, metronidazole, penicillin, and tetracycline were determined by broth dilution method. The bacterial morphology was observed with bright field microscope after incubating with sub-MIC antibiotics. The length of A. actinomycetemcomitans and F. nucleatum were increased after incubation with metronidazole; penicillin and amoxicillin. P. gingivalis were increased after incubating with metronidazole and penicillin. However, F. nucleatum showed decreased length after incubation with doxycycline and tetracycline. In this study, we observed that sub-MIC antibiotics can affect the morphology of periodontal pathogens.

Comparative Studies on Morphology of Low Temperature Ar-Plasma-treated Polymer Surfaces (알곤저온플라즈마처리된 고분자표면의 모폴로지에 대한 비교연구)

  • Seo Eun-Deock
    • Textile Coloration and Finishing
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    • v.16 no.5
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    • pp.35-41
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    • 2004
  • Poly(ethylene terephthalate), polyimide(Kapton), and polypropylene surfaces were modified with argon low temperature plasma by RF glow discharge at 240m Torr, 40W to investigate the surface morphological changes due to the plasma treatment using atomic force microscopy(AFM). Analysis of the AFM images and Ra(average roughness) revealed that the plasma treatment resulted in significant ablation on the surfaces. The morphological changes and surface roughness, however, were different depending on material characteristics such as heat stability, presence of amorphous region, swelling phenomenon, and molecular structure of repeating unit. It was assumed that polypropylene due to its tertiary hydrogen was ablated easily compared to poly(ethylene terephthalate), and that polyimide was more resistant to the ablation than PET due to rigid skeleton of imide and stable phenyl ring structure.

Changes of Protein Bodies in the Soybean Cotyledons during Early Germination (발아과정에 따른 대두단백과립의 변화에 관하여)

  • 염영중
    • Journal of Plant Biology
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    • v.20 no.4
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    • pp.151-155
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    • 1977
  • Ultrastructural changes of the protein bodies in Glycine max during early germination were studied. There were no major morphological changes in protein bodies within 3 days after the imbibition, but from the 4th day the expanse of protein bodies could be observed. In subsequent stages, the aggregation of protein bodies coalesced into a large mass and then less electron-dense material in the central part of the cell. At last it bacame highly vacuolated.

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