• 제목/요약/키워드: Morphological and cultural characteristics

검색결과 372건 처리시간 0.019초

Pepsin 저해물질을 생산하는 방선균의 분리 및 검색 (Isolation and Screening of Pepsin Inhibitor-Producing Actinomycetes)

  • 박석규;성낙계;노종수
    • 한국미생물·생명공학회지
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    • 제17권2호
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    • pp.115-120
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    • 1989
  • 세포외로 pepsin 저해물질을 생산하는 미생물을 획득할 목적으로 screening test를 실시하여, porcine pepsin에 대하여 우수한 저해력을 나타내는 방선균 1균주(GF 155-2)를 분리하였다. 각종 배지상에서 그 형태학적·배양학적·생리학적 성질을 조사하여 본 결과 그 미생물학적 특성이 Microtetraspora속과 유사하였다.

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Two Species of Penicillium Associated with Blue Mold of Yam in Korea

  • Kim, Won-Ki;Hwang, Yong-Soo;Yu, Seung-Hun
    • Mycobiology
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    • 제36권4호
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    • pp.217-221
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    • 2008
  • During 2007 survey of post-harvest diseases of yam performed in May and June, severe tuber loss caused by blue mold was observed in Iksan, Cheonbuk Province. Two species of Penicillium were isolated from the infected tubers. Based on $\beta$-tubulin gene sequence analysis, and cultural and morphological characteristics, the isolates were identified as Penicillium sclerotigenum and P. polonicum. P. sclerotigenum, which is a novel to Korea, is presently described and illustrated.

First Report of Bacterial Wilt Caused by Ralstonia solanacearum Biovar 2 Race 1 on Tomato in Egypt

  • Seleim, Mohamed A.A.;Abo-Elyousr, Kamal A.M.;Abd-El-Moneem, Kenawy M.;Saead, Farag A.
    • The Plant Pathology Journal
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    • 제30권3호
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    • pp.299-303
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    • 2014
  • This study aims to isolate and identify the causal pathogen of tomato bacterial wilt in Egypt. In 2008, tomato plants showing typical symptoms of bacterial wilt disease with no foliar yellowing were observed in Minia, Assiut and Sohag governorates, Egypt. When cut stems of symptomatic plants were submerged in water, whitish ooze was evident and longitudinal sections showed a brown discoloration in the vascular tissues. Bacteria were isolated on triphenyl tetrazolium chloride medium and fifteen isolates shown typical morphological and cultural characteristics were confirmed as Ralstonia solanacearum biovar 2 race 1. Pathogenicity tests showed that all isolates proved to be pathogenic to tomato plants, varied from 52 to 97% wilting. This is the first report of R. solanacearum biovar 2 race 1 causing bacterial wilt in tomato crop in Egypt.

Lipoxygenase Inhibitor를 생산하는 미생물의 분리 및 배양 (Isolation and Cultivation of Microorganism Producing Lipoxygenase Inhibitor)

  • 황지숙;정영기이태호
    • KSBB Journal
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    • 제8권4호
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    • pp.375-381
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    • 1993
  • 토양으로부터 세포외로 lipoxygenase inhibitor를 생산하는 균주를 screemng하여 우수한 저해능을 가지는 곰팡이 1균주 (L242H)를 분리하였고, 분리한 균주 L242H로부터 lipogenase inhibitor 생산조건을 검토하였다. 분리한 균주의 배양학적. 형태학적 특성을 조사해 본 결과 Penicillium속으로 동정되었다. Penicillium L242H의 플라스크 배양에 의한 lipoxygenase inhibitor 최적배양조건은 3.0% glucose, 0.4 % ammonium sulfate, 0.1 % potassium p phosphate (dibasic), 초기 pH 7.0이었고, 500ml용 shake flask에 배지 100ml를 넣어 8일간 배양했을 때 lipoxygenase inhibitor가 최대로 생산되었다.

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Botrytis allii에 의한 양파 잿빛썩음병 (Gray Mold Neck Rot of Onion Caused by Botrytis allii in Korea)

  • 박숙영;이동현;정희정;고영진
    • 한국식물병리학회지
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    • 제11권4호
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    • pp.348-352
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    • 1995
  • Severe gray mold neck rot of onion occurred in most farmers' fields in the southern part of Korea, and 20∼50% of onions were infected by the disease at Goheung, Chonnam, in 1994. Symptoms of the disease appeared on the lower leaves near the soil surface in late February. The symptoms initially appeared as yellowish blotch with compact gray mold on the surface of the infected leaves and developed to blast of the aboveground parts of onions. As brown to dark brown symptoms progressed around the necks of onion later, the bulbs were rotting gradually. Botrytis sp. repeatedly isolated from the lesions produced the typical symptom on the neck of healthy onion 7 days after wound inoculation of conidial suspension of the fungus. The fungus reisolated from the bulbs was identified as Botrytis allii Munn based on the morphological and cultural characteristics and pathogenicity. This is first report of a gray mold neck rot of onion in Korea.

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Erwinia carotovora subsp. carotovora에 의한 관엽식물의 새로운 세균성무름병 (New Bacterial Soft Rot of Ornamental Foliage Plants by Erwinia carotovora subsp. carotovora in Korea)

  • 최재을;이은정
    • 식물병연구
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    • 제6권1호
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    • pp.19-22
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    • 2000
  • 1998∼1999년 대전, 경기도 여주와 성남, 경남 김해, 제주도 등지의 비닐하우스에서 재배중인 관엽식물의 잎과 줄기의 무름 병징으로부터 식물병원세균을 분리·동정하였다. 베고니아(Begonia hiemalis), 바이올렛(Saintpaulia sp.), 군자란(Clivia miniata)으로부터 분리된 병원세균들을 형태적, 배양적, 생리적 및 병리학적 특성을 조사한 결과 E. carotovora subsp. carotovora로 동정되었다. E. carotovora subsp. carotovora에 의한 베고니아, 바이올렛, 군자란의 무름병은 국내에서 보고된 바가 없기에 베고니아, 바이올렛, 군자란의 세균성 무름병을 최초로 보고한다.

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난 썩음병균 Fusarium sp. KS-01의 분리 및 계통학적 분석 (Isolation and Phylogenetic Analysis of Orchid Rotting Fungus, Fusarium sp. KS-01)

  • 박인재;신광수
    • 한국균학회지
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    • 제33권2호
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    • pp.92-94
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    • 2005
  • 동양란에 썩음병을 일으키는 병원균을 분리하여 동정하였다. 병원균은 배양학적으로나 형태학적으로 Fusarium 속의 특성을 지니고 있었고, PCR 기법을 사용한 18S rRNA 염기서열을 분석결과 Fusarium 속의 균류와 매우 높은 유사도를 보였다. 계통학적 분석결과, 본 균주는 Fusarium sp. LP-A2/3(AF548314)과 가장 근접한 것으로 나타났다.

Occurrence of Soybean Sleeping Blight Caused by Septogloeum sojae in Korea

  • Hong, Sung Kee;Choi, Hyo Won;Lee, Young Kee;Lee, Sang Yeob;Shim, Hong Sik
    • Mycobiology
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    • 제40권4호
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    • pp.265-267
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    • 2012
  • Sleeping blight was observed on soybean plants grown in Yanggu, Suwon and Geumsan from 2005 to 2011. Symptoms developed on stems and pods of affected soybean plants. Five fungal isolates were obtained from the diseased plants and identified as Septogloeum sojae based on their morphological, cultural and molecular characteristics. Pathogenicity of the fungus was confirmed on soybean plants by artificial inoculation. This is the first report of S. sojae causing sleeping blight in soybean plants in Korea.

Occurrence of Anthracnose on Fruits of Asian Pear Tree Caused by Colletotrichum acutatum

  • Kim, Wan-Gyu;Hong, Sung-Kee;Park, Yeong-Seob
    • Mycobiology
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    • 제35권4호
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    • pp.238-240
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    • 2007
  • Anthracnose symptoms often occurred on fruits of Asian pear trees grown in Anseong, Naju, Seonghwan and Pyeongtaek in Korea during the harvesting period from 2000 to 2005. A total of 28 isolates of Colletotrichum sp. were obtained from the anthracnose symptoms. All the isolates were identified as Colletotrichum acutatum based on their morphological and cultural characteristics. Four isolates of the fungus were tested for pathogenicity to fruits of Asian pear tree by artificial inoculation. All the isolates induced anthracnose symptoms on the fruits by wound inoculation but not by unwound inoculation. The anthracnose symptoms induced by artificial inoculation were similar to those observed in the orchard. This is the first report of anthracnose of Asian pear tree caused by Colletotrichum acutatum.

Two Species of Endophytic Penicillium from Pinus rigida in Korea

  • Kim, Chang-Sun;Park, Myung-Soo;Yu, Seung-Hun
    • Mycobiology
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    • 제36권4호
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    • pp.222-227
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    • 2008
  • Upon studying the diversity of fungal endophytes associated with pine trees in Korea, many species of Penicillium were encountered. In this study, we report two species of Penicillium isolated from the needles of Pinus rigida. Based on ID region analysis, cultural and morphological characteristics, the two species were identified as Penicillium fellutanum and P. toxicarium, both of which are new to Korea.