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Effect of time and depth of insemination on fertility of Bharat Merino sheep inseminated trans-cervical with frozen-thawed semen

  • Kumar, Davendra;Naqvi, Syed Mohammed Khursheed
    • Journal of Animal Science and Technology
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    • v.56 no.3
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    • pp.8.1-8.6
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    • 2014
  • Background: Artificial insemination (AI) can serve as a powerful tool to the sheep owners for making rapid genetic progress of their flock. The AI in sheep is mostly performed using fresh semen with two reasons i) lambing rate following trans-cervical AI with frozen semen is limited by the inability of frozen-thawed sperm to transit the cervix and ii) the need of circumventing the cervical barrier through laparoscope aided intrauterine AI. Therefore, AI with frozen-thawed semen is not as widespread in sheep as it is in other domestic species. However, to get maximum benefits through the use of AI, frozen-thawed semen is a prerequisite because instead of high fertility, the short shelf life of fresh semen coupled with a limitation on the number of insemination doses achievable per unit time restricts the widespread use of individual sires. Therefore, in order to enhance lambing rate, a total of 240 trans-cervical artificial inseminations with frozen-thawed semen were performed in Bharat Merino ewes during autumn season either once in the evening (G-I, 10 h after onset of estrus, n = 100) or twice (G-II, 14 h and 22 h after onset of estrus, n = 140) i.e. once in the morning and again in the evening. Results: The pregnancy rate (proportion of pregnant ewes confirmed by ultrasonography at day 40) and lambing rate (proportion of ewes lambed) were higher in G-II as compared to G-I (26.4 vs 20% and 19.3 vs 10%, respectively). The difference in lambing rates was statistically (P < 0.05) significant. The depth of insemination within cervico-uterine tract had no significant effect on pregnancy and lambing rates. Conclusions: The results indicate that lambing rate in sheep following TCAI with frozen-thawed semen was significantly influenced by time of inseminations. Two inseminations after 14 and 22 h of onset of estrus enhanced the lambing rates of Bharat Merino sheep as compare to single insemination after 10 h of onset of estrus. The TCAI technique with frozen-thawed ram semen is promising and may serve as a valuable tool for genetic improvement of sheep breeds. Research efforts are going on worldwide to overcome the poor fertility following TCAI with frozen-thawed semen.

A Study on the Behavioral Characteristics of the Users and Preferences of the Bench and Pergolas in Busan Citizens' Parks (부산시민공원의 벤치 및 파고라 이용자 행태 특성 및 선호도 연구)

  • Wang, Dan;Yoon, Ji-Young
    • The Journal of the Korea Contents Association
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    • v.18 no.8
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    • pp.658-670
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    • 2018
  • Busan Citizen Park has been taken as the research object in this paper to learn about the categories and types of resting facilities such as seats and Pergolas in the park and analyze the visitors' use patterns of bench and Pergolas. In addition, the analysis of the cultural features and preferences of bench and Pergolas will provide the basic data for the future design of resting facilities. After the research on the categories and types of bench and Pergolas and the evaluation factors through literature surveys, the type, location, and number of resting facilities including bench, Pergolas, sheds, etc. in the entire park have been investigated through field surveys. In addition, the behavioral map analysis has been created through the observation of the use patterns of bench and Pergolas in the morning and afternoon of each month, and the degree of preference and satisfaction of park bench and Pergolas has been grasped through questionnaires. The research results are as follows. Among the ten types of bench and Pergolas, the citizens like the mats and awnings + mats best. The environment is the most important factor for the mats with highest score, followed by the functional and regional factors. In addition, various activities such as eating in mats and sheds that block sunlight are Korean use patterns, which is very common in Korean daily life. These results show that bench and Pergolas in urban parks are not placed arbitrarily and the layout and design of bench and Pergolas should be completed based on behavior and preferences, which are influenced by cultural characteristics.

The effect of enhancing eye-wrinkle applying traditional herb medicine cosmetics (한방화장품의 눈가 주름 개선 효과)

  • Kang, Shin-Jyung;Kim, Ae-Jung;Lee, Myoung-Sook;Lee, Yeon-Hee;Ji, Joong-Ku
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.1
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    • pp.335-340
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    • 2011
  • This study evaluated anti-wrinkle effect for eye using essence and cream containing extract of Liriope platy phyllla, Inonotus obliquus, Glycyrrhiza glabra, black ginseng, Atractylodes japonica, Poria cocos Wolf, Angelica gigas, Rehmanniae Radix Preparata and Cnidii Rhizoma, Paeonia lactiflora. 6 female adults on average at the age of 44.8, applied cream after essence under the normal conditions of use on the crow's feet area, twice a day (in the morning and evening), 2mg/$1cm^2$ for 4weeks. After 4 weeks, the replica of eye wrinkle was made and analyzed the image. The result was that eye wrinkle was decreased in R1($3.22{\pm}0.85$ to $2.38{\pm}0.32$), R2($3.08{\pm}0.83$ to $2.22{\pm}0.32$), R3($2.29{\pm}0.64$ to $2.29{\pm}0.64$), R4($0.44{\pm}0.14$ to $0.44{\pm}0.14$), and R5($0.98{\pm}0.21$ to $0.98{\pm}0.21$)(P<0.05). Therefore, oriental medicine cosmetics containing traditional herb medicine may play a role anti-wrinkle for eye.

The Effects of Belly Button Healing on EEG Activity in high school students (배꼽힐링(Belly Button Healing) 프로그램이 고등학생의 뇌파 활성도에 미치는 영향)

  • Shin, Jae-Han;Kang, Hojung;An, Seung Chan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.2
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    • pp.374-382
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    • 2017
  • The aim of this study was to evaluate the effectiveness of belly button healing by measuring the EEG activity after developing and applying belly button healing to high school students. This study was conducted on 19 high school students (8 boys and 11 girls). The students who participated in this study underwent belly button healing for about 5 minutes twice per daily (morning and evening) for 2 weeks. In this experiment, an electroencephalogram (EEG) of the subject was measured in a state of arousal and steady state and the change in EEG was observed during the stroop task. Subsequently, belly button healing was performed for about 5 minutes with a one minute break and the same procedure was then repeated. As a result of the study, the reduction of the M-Beta wave, H-Beta wave and Gamma wave was lower compared to the arousal state due to the navel healing program. The reduction of the Alpha wave and SMR wave also was decreased compared to the steady state by the navel healing program. This suggests that belly button healing has improved the concentration, immersion, and psychological stability of the subjects in stress situations. This is consistent with the participants' interviews after the research. The conclusions of this study are as follows. First, because belly button healing is effective in relieving stress in high school students, such a program will be needed for students who are experiencing academic stress. Second, because belly button healing can help increase high school students' concentration, it is recommended to implement belly button healing before addressing certain complex or difficult tasks.

Effect of Thermal Stress on Sexual Behaviour of Superovulated Bharat Merino Ewes

  • Maurya, V.P.;Naqvi, S.M.K.;Gulyani, R.;Joshi, A.;Mittal, J.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.10
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    • pp.1403-1406
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    • 2005
  • The present study was undertaken to study the effect of thermal stress on sexual behaviour of superovulated ewes. Fourteen adult Bharat Merino ewes with an average body weight of 29.4${\pm}$2.34 kg were randomly allocated into two groups of 7 each. All the animals were grazed on natural pasture in the morning and evening hours and housed in shed during night. Animals of Group-1 were housed in shed from 10:00 to 16:00 h while the animals of Group-2 were exposed to thermal stress in a hot chamber ($40^{\circ}C$ /6 h/day). All the animals were offered drinking water once a day at 16:30 h. Meteorological observations i.e. dry bulb, wet bulb, minimum and maximum temperature were recorded daily inside the shed as well as in hot chamber throughout the experimental period. For superovulation of animals, standard protocol developed at the Institute, using FSH (Ovagen 5.4 mg in eight injections) and PMSG (200 IU) was followed. Various sexual behaviour parameters (circling, tail fanning, head turning, standing and approaching to ram) and estrus incidence (onset of estrus and estrus duration) were observed in both the groups. The different estrus symptoms were graded subjectively on arbitrary scale of 0-5 where 0 representing no sexual behaviour (0%) and 5 representing maximum intensity in sexual behaviour (100%). Estrus was detected with the help of a marked aproned ram of proven vigor at six hourly intervals. The average percent values for sexual behaviour parameters recorded in Group-1 and Group-2 animals were 53.7${\pm}$3.76 vs. 41.1${\pm}$2.18 for circling, 71.8${\pm}$5.42 vs. 49.0${\pm}$4.41 for tail fanning, 64.7${\pm}$3.30 vs. 44.5${\pm}$4.34 for head turning, 90.1${\pm}$3.16 vs. 75.8${\pm}$4.02 for standing and 63.8${\pm}$4.8 vs. 41.9${\pm}$4.58 for approaching to ram. Animals exposed to thermal stress had significantly lower values of these sexual behaviour parameters. The animals kept in shed exhibited estrus earlier (25.4${\pm}$1.12 h) and duration was higher (37.7${\pm}$1.59 h) as compared to animals exposed to thermal stress i.e. 30.6${\pm}$1.16 h and 31.7${\pm}$3.57 h, respectively. The findings of the study indicate that thermal stress reduces the intensity of sexual behaviour in ewes and may result in failure of the animal to mate and conceive.

Effects of Moist Extruded Full-fat Soybeans on Gut Morphology and Mucosal Cell Turnover Time of Weanling Pigs

  • Qiao, Shiyan;Li, Defa;Jiang, Jianyang;Zhou, Hongjie;Li, Jingsu;Thacker, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.1
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    • pp.63-69
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    • 2003
  • Ten barrows, weaned at 28 days (7.2$\pm$0.1 kg BW), were used to evaluate the effects of feeding extruded full-fat soybeans on intestinal morphology and mucosal cell turnover time. All pigs were fed corn-based diets with half of the pigs receiving diets supplemented with 15.5% soybean meal and 3% soybean oil and the remaining pigs fed a diet in which the soybean meal and oil were replaced by 18.5% extruded full-fat soybeans. The pigs were individually placed in $80{\times}150cm$ metabolic cages and fed twice daily an amount approximately equal to their ad libitum intake for a period of 14 days. On day 14, pigs were weighed and then injected intraperitoneally with $^3$H]thymidine ($100{\mu}Ci/kg$ of BW, specific activity 20 Ci/mmol) 6 h after the morning meal. A pig from each treatment was killed 1, 4, 8, 16, or 24 h postinjection and intestinal tissues were collected. Daily gains for pigs fed the soybean diet and extruded full-fat soybean diet were 0.24 and 0.31 kg/day (p=0.05) with feed conversions of 1.58 and 1.39 (p=0.05), respectively. In comparison with pigs fed soybean meal, pigs fed moist extruded full-fat soybeans had a decreased crypt depth in their duodenum and cecum (p<0.1), while the villus height in the mid jejunum and ileum and the total height (villus height plus crypt depth) of the ileum and mid jejunum increased (p<0.05). The villus width in the duodenum and mid jejunum decreased (p<0.05). The number of crypt epithelial cells in the upper jejunum increased but decreased in the ileum, colon and cecum (p<0.05). The number of villus epithelial cells in the ileum and the upper and mid jejunum increased (p<0.05). The time for migration of epithelial cells in the crypt-villus column decreased (p<0.05) in all sites except the upper jejunum, ileum and cecum. The mucosal turnover rate for all intestinal sites except the upper jejunum, colon and cecum decreased (p<0.05). From these data, we conclude that inclusion of moist extruded full-fat soybeans in weanling pig diets can improve the intestinal morphology and slow the migration rate and turnover time of epithelial cells of the small intestine, especially in the mid jejunum compared with soybean meal.

The Concentrations of Ambient VOCs at Industrial Complex Area in Daegu City (대구시 산단지역 대기 중 휘발성유기화합물의 농도)

  • Kim, Eun-Kyung;Song, Hee-Bong;Park, Min-Sook;Lim, Jong-Ki;Kwon, Jong-Dae;Choi, Su-Jin;Park, Su-Kyung;Han, Gae-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.7
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    • pp.498-505
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    • 2014
  • This study was carried out to characterize the ambient VOCs concentrations of industrial complex in Daegu city. VOCs concentrations of industrial complex area appeared to be higher than those in the non-industrial complex area as following order toluene > ethylbenzene > m,p-xylenes > o-xylene but benzene and chloroform were similar to each other sites. There was seasonal variation of VOCs concentrations in the industrial complex area, higher in summer and lower in winter. In particular the industrial complex area shows that higher on weekday than weekend and higher in the daytime than in the morning or nighttime because industrial complex area influenced by the chemicals. Correlation coefficients among VOCs were confirmed that VOCs of industrial complex area were more related to each other than those of non-industrial complex area and VOCs concentrations have shown generally negative correlation with wind speed.

A Case of Micturition Syncope in a Child (소아에서 발현한 배뇨 후 실신 1례)

  • Lee, Sun Youn;Ryu, Su Jeong;Kim, Deok Soo;Kim, Young Hwue;Ko, Tae Sung;Kim, Jae Moon
    • Clinical and Experimental Pediatrics
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    • v.46 no.12
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    • pp.1274-1278
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    • 2003
  • Syncope in children and adolescents have a common occurrence according for up to 15% before adulthood. Micturition syncope, a kind of situational syncope, can be considered a form of reflex syncope. It can typically occur in healthy young men after rising from bed in the early morning who experience sudden loss of consciousness during or immediately after urination. The mechanism of micturition syncope is not completely understood, but it has been suggested that vasovagal reflex mediated bradycardia and peripheral vasodilation and decreased venous return due to Valsalva effect and standing position lead to the decrease in cerebral blood flow resulting in syncope. The causes of syncope are variable. So complete history taking, physical examination, electrocardiography, exercise stress test, echocardiography, head-up tilt table test, electroencephalography(EEG), brain magnetic resonance image and urodynamic study should be required for the diagnosis of micturition syncope. There were several reports about micturition syncope. However, literature of micturition syncope at the pediatric age has rarely been reported in Korea so far. Therefore, we report a case of a 9-year-old boy with micturition syncope with typical EEG findings of high amplitude delta wave and flattening during syncope.

A Study in Every Possible Correlation between Daily Food Intake and Growth Rate of Primary School Students (국민학교(國民學校) 아동(兒童)의 영양섭취실태(營養攝取實態)와 성장발육(成長發育)에 관(關)한 연구(硏究))

  • Ryu, Young-Sang;Kim, Sook-He
    • Journal of Nutrition and Health
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    • v.6 no.2
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    • pp.25-33
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    • 1973
  • With the object of the study id every possible correlation between daily food intake and growth rate of primary school students, aged eleven years old, the author distributed prepared questionnaires through 200 children of 5th grade in both sexes in K private primary school, Seoul. Specific object of this study were included following three points: such as daily food intake records for six days. daily food intake records for six days. school records of sample children at the end of 4th grade. physical growth index calculated by means of weight and height of sample children at the first part of the 5th grade. CR. t. F. test as well as percentage calculations were included for statistical analysis. The result of this study were as follows: 1. For the living circumstances a) Average numbers of brother and sister in sampled family were two to three. b) Approximately 50%of the children were brought up by mother's milk at their infants and about 35% of children were weaned at the age of 7 to 12 months. c) Thirty to forty percent of the respondents revealed irregulity in their meal time due to early school time in the morning. d) Monthly average expenditure for daily food reached 20 to 29% out of total income. e) The most favorit foods reflect as follows: The most favorit main dish is boiled rice mixed with other careals and side dish beaf. Fruits and juice are more popular snack among whole children. 2. Nutrition survey a) The calcium and thiamine were the most limited factors out of daily nutrients consumption. b) The total calory intake were composed of carbohydrate 63%, protein 16% and fat 21% respectively. c) The total food intake were composed of 20 to 24% of animal source of foods and 76 to 80% of plant source of foods (vegetables, grains, fruits) d) The total protein intake were composed of 46% of animal protein and 54% of others. e) The grain stuffs consumed in the largest quantity at the level of 50% out whole food groups. f) Twenty four percent of total food intake came from snack between meal time. 3. The result of statistical analysis were as follows: a) The correlation between the calory intake and plysical growth index revealed statistical significancy at the level of p<0.001. b) The correlation between the protein intake and physical growth index revealed statistical significancy at the level of p<0.001. c) The correlation between the meal time and physical growth index revealed statistical significancy at the level of 0.02

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Concentration Variation of Atmospheric Radon and Gaseous Pollutants Related to the Airflow Transport Pathways during 2010~2015 (대기 라돈 및 기체상 오염물질의 기류 이동경로별 농도변화: 2010~2015년 측정)

  • Song, Jung-Min;Kim, Ki-Ju;Bu, Jun-Oh;Kim, Won-Hyung;Kang, Chang-Hee;Chambers, S.
    • Journal of Korean Society for Atmospheric Environment
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    • v.34 no.2
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    • pp.321-330
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    • 2018
  • Concentrations of the atmospheric radon and gaseous pollutants were measured at the Gosan site on Jeju Island from 2010 to 2015, in order to observe their time-series variation characteristics and examine the concentration change related to the airflow transport pathways. Based on the realtime monitoring of the atmospheric radon and gaseous pollutants, the daily mean concentrations of radon ($^{222}Rn$) and gaseous pollutants($SO_2$, CO, $O_3$, $NO_x$) were $2,400mBq\;m^{-3}$ and 1.3, 377.6, 41.1, 3.9 ppb, respectively. On monthly variations of radon, the mean concentration in October was the highest as $3,033mBq\;m^{-3}$, almost twice as that in July ($1,452mBq\;m^{-3}$). The diurnal variation of radon concentration shows bimodal curves at early morning (around 7 a.m.) and near midnight, whereas its lowest concentration was recorded at around 3 p.m. Several gaseous pollutants($SO_2$, CO, $NO_x$) showed a similar seasonal variation with radon concentration as high in winter and low in summer, whereas the $O_3$ concentrations had a bit different seasonal trend. According to the cluster back trajectory analysis, the frequencies of airflow pathways moving from continental North China, East China, Japan and the East Sea, the Korean Peninsula, and North Pacific Ocean routes were 36, 37, 10, 13, and 4%, respectively. When the airflow were moved to Jeju Island from continental China, the concentrations of radon and gaseous pollutants were relatively high. On the other hand, when the airflows were moved from North Pacific Ocean and East Sea, their concentrations were much lower than those from continental China.