• Korean Journal of Environment and Ecology
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• v.22 no.4
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• pp.435-441
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• 2008

This study conducted a survey on the moulting sequence subsequent to age of Haliaeetus pelagicus raised in captivity at the Ornithology Laboratory attached to Kyungsung University for about six years from November, 2000 until July, 2006. The survey indicated that the moult of rectrices usually began in July and continued until April of the next year and most of the rectrices were replaced by one-time moult. Usually, about two thirds of the tail feathers were replaced while the rest were replaced no later than April of the next year, and the moult also continued during the wintertime. The total number of rectrices was 14, and the moult progressed alternately on a systematic basis. The progress of the moult for female & male was made on four stages and three stages respectively and the characteristic shown on every stage of the moult was that the left & right tail feathers progressed symmetrically and not until one stage of progress almost completed did the next stage began. The color of the juvenile steller's sea-eagle was dotted with black spots on its original white color and there existed regular black belt on its feather's fringes; however, it was difficult to identify its age by tail feathers only because there was almost no difference in color between feathers ranging from the first to the third generation(1st-3rd summer feathers). In addition, this research took the different amounts of black-speckled pattern appearing by individual into consideration. There existed slight black speckles in white color feathers of the fourth generation(the 4th summer feathers) while showing a big difference compared to the 3rd generation feathers. The 5th generation feathers[the 5th summer feathers]were found to be equipped with perfect tail feathers having virgin white of a steller's sea-eagle after completing its 4th molt. When observing a steller's sea-eagle in the open air, it is necessary for an observer to have a deliberate examination in judging its age belonging to the 1st-3rd generation feathers, and it is considered that the changes of other parts of feathers should be also observed besides tail feathers.

• Natural Product Sciences
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• v.20 no.3
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• pp.160-169
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• 2014

44 compounds and 9 minerals were isolated from and detected in the New Zealand deer velvet antler Cervus elaphus var. scoticus L$\ddot{o}$nnberg. The chemical structures of (1 - 26) were identified on the basis of the spectroscopic methods and comparisons with literature, respectively. The structures were identified as cholesterol (CS, 6), 7-keto-CS (7), $7{\beta}$-hydroxy-CS (8), and $7{\alpha}$-hydroxy-CS (9), and included 12 steroid $3{\beta}$-O-(palmitic/stearic/myristic acid esters; PM/SA/MS) [CS-$3{\beta}$-O-PM (1 - 1), CS-$3{\beta}$-O-SA (1 - 2), CS-$3{\beta}$-O-MR (1 - 3), 7-keto-CS-$3{\beta}$-O-PM (2 - 1), 7-keto-CS-$3{\beta}$-O-SA (2 - 2), 7-keto-CS-$3{\beta}$-O-MR (2 - 3), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-SA (3 -1), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-PM (3 - 2), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-MR (3 - 3), $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-SA (4 - 1), $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-PM (4 - 2), and $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-MR (4 - 3)], dinonyl phthalate (5), 8 nucleic acids analogues [uracil (10), deoxyguanosine (11), deoxyuridine (12), uridine (13), deoxyadenosine (14), adenosine (15), inosine (16), and guanosine (17)], and the 9 free amino acids [L-phenylalanine (18), L-isoleucine (19), L-leucine (20), L-tyrosine (21), L-valine (22), L-proline (23), L-threonine (24), L-alanine (25), and L-hydroxyproline (26)]. Also, there are 8 kinds of amino acids [asparagine, serine, glutamine, glycine, histidine, arginine, methionine, and lysine], 2 sialic acids [N-acetylneuraminic acid (27), ketodeoxynonulosonic acid (28)], and 9 minerals [Na > K > Ca > Mg > Fe > Zn > B > Al > Cu] were detected from the autoaminoacid analyzer and ICP spectrometer, HPAEC-PAD/HPLC-FLD, respectively. 9 kinds of oxycholesterol-$3{\beta}$-O-fatty acid ester (2 - 1, 2 - 2, 2 - 3, 3 - 1, 3 - 2, 3 - 3, 4 - 1, 4 - 2, and 4 - 3) and 3 nucleic acids (12, 14, and 15) were isolated from the velvet antler for the first time. 6 kinds of steroids (7, 8, 9, 2 - 1, 3 - 1, and 4 - 1) were examined for their anti-proliferative effects against L1210, P388D1, K562, MEG-01, KG-1, MOLT-4, A549, HepG2, MCF-7, SK-OV-3, and SW-620 cancer cell lines. They showed anti-proliferative effects with $IC_{50}$ values of 0.06, 2.16, 2.42, > 50.0, 1.66 and $8.31{\mu}M$ against L1210, while the values were 24.05, 9.44, 5.22, 0.25. 9.48 and $49.77{\mu}M$ against P388D1, respectively. The others were inactive.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.349-356
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• 2013

A set of experiments were conducted to determine the zero temperature and total effective temperature for the summer diapause and post-diapause development of Matsucoccus thunbergianae Miller et Park (Hemiptera: Margarodidae) which infests the Japanese black pine, Pinus thunbergii. The diapausing first instar nymphs were kept in cool storage during three separate times, each starting from May 4th, June 19th, and August 15th of 2002. Cool storage temperatures were 2.5, 5.0, 7.5, 10.0, 12.5 and $15.0^{\circ}C$. The nymphs were chilled for 10, 20, 30 or 40 days in the first two sets of experiments. In the third experiment, nymphs were chilled for 3, 6, 9 or 12 days. Molting into the second instar nymphs was examined every 10 days, starting at 20 days after taken out from the cool storage. Optimum temperature range of the diapause development was between 7.5 and $10^{\circ}C$, where diapause development was completed in 40, 20, and 6 days by the insects chilled from May 4th, June 19th and August 15th, respectively. Comparing the three sets of experiments with different chilling periods, zero temperature for diapause development was calculated as $29^{\circ}C$. Effective temperature for diapause development was 964 degree days, and it was estimated that nymphs completed their diapause development by September 8th in nature. Under natural temperature conditions >50% eclosion into the second instar occurred on November 9th. Zero temperature for post-diapause development was $10^{\circ}C$, and total effective temperature for post-diapause development until the molt into the second instar was 391 degree days.

• Journal of Life Science
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• v.19 no.1
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• pp.58-64
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• 2009

This study was conducted to know the molting sequence and the aging points of flight feathers of steller's sea eagles (Haliaeetus pelagicus). For this study, two captive immature steller's sea eagles raised at the Ornithology Laboratory attached to Kyungsung University were surveyed for five years from Nov. 2000 to Nov. 2005. The survey indicated that the first molting began in July of the second year, and the primaries of P1-3, the secondaries of S18-19 (female), S17-18 (male), and S1 and S4 were replaced by one-time with second generation feathers. Generally molting stopped during the winter period, but a few feathers continued to molt during the winter. The two secondaries of S18-19 (female) and S17-18 (male) always molted every year but some of the juvenile secondaries (male: S10, S11, etc) retained for 2 or 3 years. In the molting order of primaries, the first molting started at P1 and it proceeded to P10 of outside. In the secondaries, the first molting started at S17(male) and S19(female), and it proceeded to outside. After that molting it started at S1 and proceeded to inside. In the other secondaries, the pattern of molting which proceeded in the mid-part of the secondaries was usually beginning in several different points at the same time. The molting seemed as if it depends on both the conditions of the individuals and the environment, so it was very difficult to explain the molting pattern in the mid-part of the secondaries. The longer quills (P7, P8) required for more than 68 days to develop. In the comparison of the length in the remiges between the first and the second generation feathers, the first generation feathers were the larger than that of the second. And the reduction of the length between the second and the third generation feathers was a few. The reduction of the length between the third and the fourth generation feathers was slight. The juvenile primaries were dark brown with a whitish base, which could be observed until the second or the third generation feathers (in their third or fourth winter plumage).

• Korean Journal of Poultry Science
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• v.35 no.4
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• pp.375-380
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• 2009

This study was conducted to investigate the effect of feeding induced molting on the visceral organs and blood component profile in laying hens and designed to test 400 flocks of 60 week old Leghorn laying hens for 34 weeks. A total of four molting treatment methods by including the molted with customary molting by fasting method (c), feeding single diet of corn (T1), feeding single diet of wheat bran (T2) and feeding single diet of alfalfa meal (T3) were tested, and each treatment was repeated for 5 times, and 20 laying hens were randomly assigned in an cage for each repeat. As the result of the experiment, ovary was $2.03{\sim}6%$ and oviduct was $2.51{\sim}3.47%$ in visceral organs for body weight at pre-molting term, but there was no significant difference. At post-molting, no significant difference was found, ovary was $0.25{\sim}0.41%$, uterus of control, T1, T2 and T3 was 1.12%, 0.82%, 0.48% and 0.90%, respectively. T2 was significantly lower than control, T3 (p<0.05) at the 50% of egg production. Ovary was $2.20{\sim}2.60%$ and oviduct was $2.98{\sim}3.45%$. In addition, ovary was $2.65{\sim}3.01%$, oviduct was $3.23{\sim}3.64%$ at the peak egg production, but there was no significant difference by non-feeding and feeding molting treatments. In blood component profile, cholesterol was $179.8{\sim}245.7\;mg/dL$ at pre-molting, but there was no significant difference and at post-molting, concentration of cholestrol in control, T1, T2 and T3 was 353.6, 229.1, 261.8 and 300.6 mg/dL, respectively. T1 was significantly lower than control and T3 (p<0.05). In addition, first laying day was $228.1{\sim}271.8\;mg/dL$, 50% of egg production was $236.5{\sim}284.8\;mg/dL$, there was no significant difference. Concentration of cholestrol in control, T1, T2 and T3 was 324.1, 591.6, 363.0 and 315.6 mg/dL, respectively, at the peak egg production period. T1 was significantly higher than other treatment (p<0.05).