• Food Science and Biotechnology
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• 제17권5호
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• pp.953-958
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• 2008

The object of this study was to investigate the immune-enhancing effects of Chlorella vulgaris (CV) on a deteriorated immune function by a protein-energy malnutrition (PEM) diet. Unicellular algae, CV were used as a biological response modifier. Male C57BL/6J mice were fed for 15 days with standard diet or a PEM diet, which is associated with decreased host immune defense. After 8 days, mice in the PEM diet group were orally administered by 0.05, 0.1, and 0.15 g/kg body weight of CV or distilled water. Nutritional parameters, and interferon (IFN)-$\gamma$ levels were significantly increased in the blood serum of the CV (0.15 g/kg)-treated group (29.6$\pm$2.8 pg/mL) compared to the non-treated PEM group (4.1$\pm$0.4 pg/mL, p<0.05). In addition, cell proliferation and production of cytokines were investigated via a CV (0.01, 0.1, and 1 mg/mL) treatment using a human T cell line MOLT-4 cell. The CV treatment (1 mg/mL) significantly increased the production of both IFN-$\gamma$ and interleukin (IL)-2 (51.3$\pm$3.4 and 285.9$\pm$18.8 pg/mL, respectively) compared to the control (51.3$\pm$3.4 and 442.6$\pm$14.3 pg/mL, respectively), but did not affect the production of IL-4. These results suggest that CV may be useful in improving the immune function.

• 한국식품영양과학회지
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• 제41권12호
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• pp.1671-1676
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• 2012

9 종류의 종자 중 대식세포를 자극하여 NO 생성능이 높은 메밀, 민들레, 봉선화, 해바라기 4종의 종자를 선별하였다. 선별된 4종의 종자 추출물이 RAW 264.7 세포를 활성화시켜 면역을 증진시키는 효과를 알아보기 위해 RAW 264.7 세포와 T세포를 이용하여 면역 활성능 관련 지표를 조사하였다. 4종의 종자를 대식세포에 처리하였을 때 면역 활성의 지표가 되는 NO, cytokine(TNF-${\alpha}$, IL-$1{\beta}$, IL-6, IL-10)의 생성이 추출물을 처리하지 않은 대조군에 비해 증가되었고, Molt-4 세포에 처리하였을 때 대조군에 비해 세포가 증식되었다. 이와 같은 결과는 종자 추출물을 섭취하였을 때 외부로부터의 어떠한 자극이 있기 이전에 체내의 모든 조직에 분포하면서 1차적으로 이물질을 제거하는 대식세포를 자극하여 cytokine 등의 면역매개물질을 생성하여 인체의 비특이적 면역반응을 증가시킴으로써 항원을 공격, 제거하는 등의 작용을 통해 자연 면역반응에 있어 중요한 역할을 할 수 있을 것으로 판단된다.

• 한국식품영양과학회지
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• 제41권11호
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• pp.1521-1527
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• 2012

본 연구에서 12종류의 생약재 중 대식세포를 활성화시켜 NO 생성능이 높은 감초, 건지황, 당귀, 도라지, 목천료 5종의 생약재를 선별하였다. 선별된 5종의 생약추출물의 성분을 실험한 결과 단백질, 다당 물질의 함유량이 감초(58.1%), 건지황(90.4%), 당귀(87.4%), 도라지(41.3%), 목천료(85.7%)로 대부분 고분자 물질이 함유되어 있는 것으로 나타났다. 생약추출물이 대식세포를 활성화시켜 면역을 증진시키는 효과를 알아보기 위해 RAW 264.7세포와 T세포를 이용하여 면역활성능 관련 지표를 측정한 결과 5종의 생약재를 RAW 264.7 세포에 처리하였을 때 면역 활성의 지표가 되는 NO, cytokine(TNF-${\alpha}$, IL-$1{\beta}$, IL-6, IL-10)의 생성이 추출물을 처리하지 않은 대조군에 비해 증가되었고, Molt-4 세포에 처리하였을 때 대조군에 비해 세포가 증식되었다. 이와 같은 결과는 고분자 물질인 단백질과 다당으로 이루어진 생약추출물을 섭취 시 외부로부터 침입한 항원이 들어오기 이전에 면역세포를 자극하여 활성을 증가시켜 면역매개물질을 생성하여 인체의 비특이적 면역반응을 증가시킴으로써 항원을 공격, 제거하는 등의 작용을 통해 자연 면역반응에 있어 중요한 역할을 할 수 있을 것으로 보인다.

• 한국식품과학회지
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• 제34권3호
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• pp.505-509
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• 2002

천마의 methanol 추출물로부터 VHR DS-PTPase 저해 물질을 분리하여 이를 HREI-MS와 $^1H-NMR$, $^{13}C-NMR$, DEPT 등의 기기 분석 자료에 의하여 baicalein으로 구조를 동정 하였다. 이 물질은 VHR에 대하여 $2.4{\mu}M$의 $IC_{50}$값을 나타내었고 T-cell PTPase나 PPase 1과 같은 다른 단백질 탈인산화 효소에 대하여는 저해 활성을 나타내지 않았다. 또한 7종류의 인간 암세포주(흑색종 세포주인 LOX-IMVI, 폐암 세포주인 NCI H23과 A549, 대장암 세포주인 HCT 116와 SW 620, 전립선암 세포주인 PC-3와 백혈병 세포주인 MOLT 4F)에 대한 세포독성을 조사하여 본 결과 $5.26{\sim}12.93\;{\mu}g/mL$에서 $GI_{50}$값을 나타내었다.

• 동의생리병리학회지
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• 제17권6호
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• pp.1441-1447
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• 2003

The purpose of this research was to investigate the effects of Chungjokupye-tang(CJKPT) on the anti-carcinogenic action. The cell viability of mouse spienocytes and thymocytes were enhanced by the addition of CJKPT. CJKPT were increased of splenic and thymic T lymphocytes, such as T/sub H/ cells were markedly increased by the treatment of CJKPT in vivo. CJKPT treatment induced the apoptotic cell death of Jurkat and HL60 leukemia cells. CJKPT reduced mitochondrial transmembrane potential and increased the expression of ICE, c-myc and p53 gene in Molt-4cells dose dependant manner. These results suggest that CJKPT have an anti-carcinogenic action via immunoregulatory mechanism.

• Journal of Microbiology
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• 제40권1호
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• pp.70-76
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• 2002

Proviral DNAs from HIV-1-infected CD4+ T cells (Molt/LAV cells) were amplified and detected in infected individual cells using polymerase chain reaction and in rifu hybridization. In this in situ PCR, three parameters were considered to achieve effective amplification and retention of amplificants inside the cells by making high molecular weight PCR products intracellularly, forming agarose matrix against the cells, and maintaining the appropriate PCR temperature profile. Over the cycles of ampliHcationl tailed primers with complementary overhanging sequences at their 5' sides manufactured high molecular weight products by using short primary products as a repeating unit. Agarose matrix could prevent the diffusion of the amplificants from the cells. Use of Thermanox coverslip inside the PCR tube offered target cells a similar temperature profile to that of conventional PCR in solution.

• 한국자원식물학회지
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• 제30권6호
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• pp.662-669
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• 2017

Rubia cordifolia has been used to treat diseases for many years in China and India. Although the biological properties and major compounds of R. cordifolia have been extensively studied, the underlying mechanisms of its biological effects remain elusive. In terms of immunological effects, anti-inflammation effect of macrophage (Raw 264.7) simply has been reported. In this study, R. cordifolia was extracted in 70% ethanol and the extract did not affect to macrophage (Raw 264.7) pro-inflammation and T cell (Molt-4). However, in mast cell (RBL-2H3), it showed inhibition of degranulation. The inducing inhibitory effect on degranulation was related to concentration dependent variation in phosphorylation of ERK-1/2 and upregulating the JNK phosphorylation in RBL-2H3 cells. Based on these data, we concluded that R. cordifolia newly have anti-allergenic effects in RBL-2H3 and might be used as a therapeutic agent to treat or prevent allergic diseases such as asthma and atopic dermatitis.

• 마이크로전자및패키징학회지
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• 제8권4호
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• pp.53-57
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• 2001

사출성형공정에서 미성형은 가장 빈번하게 발생하는 성형불량중의 하나이다. 이러한 미성형을 해결하기 위해 가상 경제적인 효율적인 방법은 공정조건을 개선하는 일이다. 그러나 대부분의 경우 사출 전문가의 오랜 기간 축적된 경험과 지식에 의존하기 때문에 여러 번의 시행착오을 겪어야 한다. 따라서 본 논문에서는 퍼지 알고리즘을 기반으로 하여 시스템에 적합한 공정조건을 찾는데 있어 시행착오를 줄이고, 또한 비전문가도 쉽게 적용학 수 있는 지능형 공정조건 생성 시스템을 제안하였다.

• 한국환경보건학회지
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• 제28권4호
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• pp.17-22
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• 2002

In crustaceans, as in other arthropods, the molt cycle and the physiological process of growth are controlled by molting hormones (MH) which are steroid hormones, the ecdysteroids. Ecdysteroids are major arthropod hormones which control both development (embryonic and larval molts, metamorphosis) and reproduction. The purpose of the present study was to evaluate both fenarimol and methoprene for embryotoxicity to daphnids. The embryotoxicity associated with each compound was assessed to discern whether the embryotoxicity of methoprene might be due to ecdysone agonist and the ecdysone antagonistic effects of fenarimol on Daphnia embryo. Exposure of daphnids for three weeks to 50 M methoprene resulted in a significantly high incidence of offspring that exhibited general toxicity. This exposure concentration had significant effects on the overall number of embryo death. However, exposure to 3 or 1 $\mu$M fenarimol were no significant effects on the embryo toxicity. The incidence of both of these toxicity increased with methoprene exposure. This observation suggest that methoprene showed embryonic general toxicity during embryo development, while, only fenarimol showed weak general toxicity with early stages of embryonic development.

• 약학회지
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• 제31권5호
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• pp.253-265
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• 1987

To develop hybridomas secreting monoclonal antibodies to be used as unlimited sources of reagents indispensable for the diagnosis and treatement of leukemic malignancy, a monoclonal antibody was generated to human pre-T leukemia cells (Jurkat). Hybridomas were produced against Jurkat cell line by fusing spleen cells from hyperimmunized mice with murine plasmacytoma cells (P3$\times$63Ag8. V653). One monoclonal antibody derived from this fusion, designated DMJ-2 was reactive with T-cell lines (Jurkat, Molt-4 and RPMI-8402) and normal peripheral E-rosette forming T cells, but unreactive with B-cell lines (Daudi, Nalm-6) and non-T, non-B cell line (K562). Conclusively DMJ-2 reactive with mature and immature T-lineage lymphoid cells.