• 제목/요약/키워드: Molecular Biology

검색결과 9,859건 처리시간 0.037초

Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis

  • Xiao, Qi;Hu, Yuhong;Yang, Xiaohong;Tang, Jianna;Wang, Xiaoshuang;Xue, Xiaomin;Li, Mengxue;Wang, Minjing;Zhao, Yinan;Liu, Jingze;Wang, Hui
    • Parasites, Hosts and Diseases
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    • 제58권2호
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    • pp.161-171
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    • 2020
  • The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.

Bacterial Fruit Rot of Apricot Caused by Burkholderia cepacia in China

  • Fang, Yuan;Li, Bin;Wang, Fang;Liu, Baoping;Wu, Zhiyi;Su, Ting;Qiu, Wen;Xie, Guanlin
    • The Plant Pathology Journal
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    • 제25권4호
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    • pp.429-432
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    • 2009
  • An unreported disease of apricot was observed in orchards in Zhejiang province, China. Symptoms started as water soaked lesions on the fruit surface. Later, water-soaked areas developed and spread to the entire fruit, resulting in soft rot of the whole fruit. The causal organism isolated from symptomatic fruits was identified as Burkholderia cepacia based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 16S rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto fruits of apricot. In addition, European plum, Japanese plum, nectarine and kiwifruit were susceptible to the B. cepacia pathogen. However, the B. cepacia pathogen failed to cause any visible symptoms when it was inoculated onto 16 other fruits. This is the first report of a bacterial disease of apricot caused by B. cepacia in China.