• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.33 no.3
• /
• pp.189-196
• /
• 2007

In order to investigate the potential of very low molecular weight hyaluronic acid(oligo HA) as a cosmetic ingredient, we first measured its cytotoxicity in fibroblast, keratinocyte, and SIRC cell lines. For efficacy test, its moisturizing effect and penetration rate were evaluated in an artificial skin system and Caco-2 cells. Oligo HA did not show any cytotoxicity at a concentration of 300 ${\mu}g/mL$ in fibroblasts and 1,000 ${\mu}g/mL$ in keratinocytes but it showed weak proliferation. In vitro ocular test, oligo HA showed negligible cytotoxicity at the maximum concentrations used(2,000 ${\mu}g/mL$) in SIRC cells. In the test of the single and repeated cutaneous applications, oligo HA under occlusive patch did not provoke any cumulative irritation and sensitization. Oligo HA at a concentration of 0.01 % exhibited a more potent moisturizing effect than hyaluronic acid at a concentration of 0.01 %. In the permeability test using artificial skin and Caco-2 cell lines, hyaluronic acid(M.W. $1.1{\times}10^6$) was hardly observed in the down medium of the inserts. On the other hand, oligo HA(M.W. 5,000) was detected in the down medium up to 16.0 % at 6 h in Caco-2 cell culture and up to 90 % at 6 h in an artificial skin system. These results suggest that oligo HA could be useful as an active ingredient for cosmetics.

• Journal of the Korean Applied Science and Technology
• /
• v.30 no.2
• /
• pp.272-279
• /
• 2013

The fermented puer tea leaves were high concentrated extracted with 50% ethanolic solution in this study. Also, evaluating the anti-oxidative activity and anti-aging effect of this extract, we expected to apply the cosmetic industry. The yield of fermented puer tea extract was 17.9%. The total polyphenol content was 37.5%, tannin content was 7.5%, polysaccharide was 17.9%, unknown compound was 22%, and water content was 8%. Anti-oxidative activity (in-vitro) of fermented puer tea extract by DPPH method was $33.7{\pm}1.8%$ in 30mg/mL, $39.4{\pm}2.2%$ in 50mg/mL respectively. Therefore, we could know that anti-oxidative activity of fermented puer tea extract was effect higher than tocopheryl acetate and greentea extract. The collagen synthesis activity (in-vitro) of fermented puer tea extract was increased with $102.9{\pm}9.9%$ in 1 mg/mL, $111.5{\pm}9.9%$ in 5 mg/mL, $122.7{\pm}12.2%$ in 10 mg/mL, $131.5{\pm}13.7%$ in 30 mg/mL (*p-value£0.05, n=3). Skin moisturizing activity of fermented puer tea extract after application 8 hours was increased 38.5% higher than control samples both tocopheryl acetate and greentea extract. Total moisturizing effect was increased about 32.7% compared to before treatment. Fermented puer tea extract of this study can be applied to the skin care cosmetics industry.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.1
• /
• pp.74-80
• /
• 2015

Skin is the soft outer covering of vertebrates that provides protection from pathogenic infection, physical damage, or UV irradiation, and controls body temperature and water content. In this study, we examined the effects of oral intake of kimchi-derived Lactobacillus plantarum K8 lysates on skin moisturizing. In an in vitro study, we observed that the hyaluronic acid content increased in HaCaT cells treated with L. plantarum K8 lysates. Oral administration of L. plantarum K8 lysates effectively attenuated the horny layer formation and decreased epidermal thickening in DNCB-treated SKH-1 hairless mice skin. The damage to barrier function was reduced after 8 weeks of oral administration of L. plantarum K8 lysates as compared with that in the atopic dermatitis mice. For the test with volunteers, we manufactured experimental candy containing 2.1% L. plantarum K8 lysates, while control candy did not contain bacterial lysate. A significant increase in hydration in the experimental candy-administered group as compared with the control candy-administered group was observed on the face after 4 and 8 weeks, and on the forearm after 4 weeks. Decreases in horny layer thickness and TEWL value were observed on the face and forearm of the experimental group. Together, the in vitro cell line and in vivo mouse studies revealed that L. plantarum K8 lysates have a moisturizing effect. A clinical research study with healthy volunteers also showed an improvement in barrier repair and function when volunteers took L. plantarum K8 lysates-containing candy. Thus, our results suggest that L. plantarum K8 lysates may help to improve skin barrier function.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.30 no.3 s.47
• /
• pp.369-375
• /
• 2004

In this study, liquid crystalline (LC) is formed using Gemini surfactant (GS) type and moisturizing effect in vivo is measured. $3.0\;wt\%$ of sodium dicocoyl ethylene diamine (PEG)-15 sulfate (SCD-PEG-15S) is used as GS and $4.0\;wt\%$ of hydrogenated dimer acid esters (HDAE) as booster. For stabilizers, $2.0\;wt\%$ of behenyl alcohol (BA) and $1.0\;wt\%$ of Iyso-lecithin (LyL) are utilized. It is stabilized in pH from 4.0 to 10.5 and the best condition is in pH 6.5. The value of viscosity is $8,000\pm500$ cP. The most excellent particles are formed within the range of 4.0 to 15.5 um. Formed LC is observed around LC particles using polarization microscope. It is also observed that lamellar gel network structure is formed around LC particles. Moisturizing effect is improved by $13.6\%$ (P<0.05) compared to control when measured 30 min later after coating samples. After 1 h, moisturizing effect is improved by 1$12.6\%$ (P<0.05) than control while showing $28.3\%$ (P<0.05) of improvement after 4 h. These results may be caused from that manufactured LC forms lamellar structure so that it has better water-holding ability and absorbance of oil increases. This formula could be utilized by delivery system (DS) on skin so that this technology can be applied for manufactuing pharmaceuticals and cosmetics.

• Journal of the Korea Convergence Society
• /
• v.11 no.5
• /
• pp.241-251
• /
• 2020

This study was performed to develop the cosmetics containing extract of U.davidiana, which has been confirmed as a functional ingredient of anti-microbial, anti-oxidant, and anti-inflammatory effects, and also to evaluate the improvement effects of dry and sensitive skin such as atopic dermatitis. Toxicity tests of the Ulmus davidiana supercritical fluid residue 60% EtOH extracts(USCFR), Ulmus davidiana supercritical fluid extracts(USCF) and preliminary formulations, DKBP-1903(gel), containing the USCFR and USCF were determined to be 'Non-toxic'. Based on this, the stability test of the lotion, cream, gel formulation was conducted and the results showed that cream and gel formulation were stable. A 48-hour moisturizing sustainability test and the improvement test on the pruritus caused by dryness were selected for the cosmetic efficacy test to evaluate the improvement of atopic dermatitis and as a result both cream and gel have been found to be relatively safe moisturizers that can help dry and sensitive skin.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.47 no.3
• /
• pp.219-226
• /
• 2021

Gastrodia elata has a very low pollination rate in natural state, and even in artificial cultivation, there are very few individuals that bloom due to the degeneration, so little studies have been conducted. This study confirmed that the potential as a cosmetic ingredient by evaluating the antioxidant activity through the evaluation of DPPH radical scavenging activity, anti-inflammatory activity through the inhibitory effect on nitric oxide production, and the moisturizing activity through the effect on promoting hyaluronic acid production by artificially flowering G. elata flower. It was also confirmed that the appearance rate and flowering rate of G. elata harvested in spring were high, and the content of gastrodin was 0.36%. The IC50 value of G. elata flower extract was 0.045% and it was confirmed that G. elata flower extract had higher radical scavenging activity than G. elata root extract. The NO production inhibitory activity against the flower extract showed a significant inhibitory effect from 1% to 83.2%. Hyaluronic acid production promotion efficacy was not confirmed in the G. elata root extract, but the production rate increased with concentration dependence in the flower extract, and it was the highest at 46.9% when 0.02% treatment was performed. Based on the above research results, it is judged that G. elata flower extract has high potential for use as an antioxidant, anti-inflammatory, and skin moisturizing cosmetic ingredient.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.48 no.3
• /
• pp.235-244
• /
• 2022

In this study, the cell viability, radical scavenging ability, elastase activity, moisturizing activity, and antimicrobial activity of mixed fermented extract of vitamin tree fruit, acai palm fruit, mango, lemon, apricot and blueberry as an active ingredient were measured. The experiment was performed at three concentrations(0.5 / 1.5 / 2%), since cytotoxicity was not shown at these three concentrations. Based on this, a radical scavenging experiment was conducted to compare with L-ascorbic acid, a representative raw material of an antioxidant material. The results showed that the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability was 95.1 ± 0.6%, 94.3 ± 0.7%, 95.3 ± 0.6%, 95.1 ± 0.7%, 95.1 ± 0.3%, 95.5 ± 0.3%, and 95.4 ± 0.4% respectively, when the concentration of the mixed fermentation extract was 2%. As the concentration increased at the three concentrations (0.5/1.5/2%) and the content of blueberry extracts increased, sample 7 showed excellent elastase activity at a concentration of 2% to 0.9 cm, such as retinol crystal, and moisturizing activity was also found to be 71.5%, 75.6%, and 81.6%, respectively. Particularly, at the 2% concentration of the mixed fermented extract, the antibacterial activity effect on Propionibacterium was very excellent in sample 7. Therefore, it is considered that it is worth developing the mixed fermented extracts of 2% concentration as a cosmetic component with antioxidant, moisturizing, and antibacterial properties.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.49 no.4
• /
• pp.355-364
• /
• 2023

Lips have a unique desquamation process and moisturizing properties that are structurally different from ordinary skin. In particular, the turnover cycle of the stratum corneum is fast and the outermost stratum corneum is thin, so the amount of keratin is relatively high, and there are no skin appendages, so it is very vulnerable to maintaining moisture. In this study, we set three targets for lip care : stratum corneum, moisture, and barrier, and aimed to identify the potential of three target-specific ingredients for lips improvement. We confirmed the exfoliating, moisturizing, and barrier improvement efficacy of three target-specific ingredients which also have mild exfoliating effect. Specifically, we verified that Bacillus clausii extract improved skin exfoliation, gluconolactone improved skin moisture retention, and serine reinforced skin barrier function. To test the in vivo efficacy of the complex composed of three target-specific ingredients on the human lips, the lip balm manufactured with non-irritating range of concentration was applied, and we confirmed that it was effective in improving lip exfoliation·moisturizing·elasticity·wrinkles. Consequently, it was confirmed that the beauty and health indicators of the lips could be improved through the exfoliation-moisturization-barrier care of the lips and these ingredients were applied to LG H&H LIPCERIN products.

• Journal of Oral Medicine and Pain
• /
• v.20 no.1
• /
• pp.127-131
• /
• 1995

A patient who showed atypical orofacial pain after an endodontic treatment for a long time, was treated with non-analgesics such as vitamin $B_1, B_2, $ and Oral Balance, moisturizing gel. 1. It is important to manage xerostomic condition properly in the treatment of patients who have atypical orofacial pain 2. Simultaneous administration of vitamin $B_1 and B_2$ was effective in increasing pain threshold of the patient.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.38 no.3
• /
• pp.197-200
• /
• 2012

To develop lipstick products with superior moisturizing effect using moisture base, bound water capacity and water holding capacity were investigated by DSC. Bound water capacity of the lipstick sample excluding moisture base is found to be 5.12 %. DSC measurement showed that bound water capacity and the concentration of moisture base were positively correlated. Additionally, DSC measurements and in vitro effective water holding capacity measurement showed similar patterns. Therefore, measuring of bound water capacity by DSC can predict the moisturizing effect of lipstick products.