• 한국미생물·생명공학회지
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• 제40권3호
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• pp.226-236
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• 2012

갓김치로부터 분리한 probiotic L. acidophilus GK20 및 L. paracasei GK74를 단독 혹은 혼합 배양하여 제조한 요구르트를 저장하는 동안 이화학적 및 미생물학적 특성과 스타터의 위산이나 담즙산에 대한 저항성, Caco-2 cell에 대한 부착능, 항균, 항산화 및 효소적 활성에 대한 prebiotic FOS(fructooligosaccharide)의 영향을 살펴보았다. FOS를 첨가했을 때 요구르트 내의 스타터 균수, 총산도 및 점도는 유의하게 높아짐과 동시에 pH는 감소되었다(p<0.05). 또한 E. coli ATCC 11229와 S. enteritidis ATCC 13076은 probiotics 스타터가 생산한 항균물질에 의해 저해되었으며, FOS를 첨가한 synbiotic 요구르트의 항균활성은 더욱 증가되었다. 게다가 FOS (1.0%)를 첨가하여 혼합 스타터로 발효시킨 요구르트에서 가장 높은 단백질 분해능 ($1.06{\pm}0.06$ unit) 및 ${\beta}$-galactosidase 활성 ($20.14{\pm}0.31$ unit)을 나타내었다. 하지만 비록 L. acidophilus GK20과 L. paracasei GK74 모두 장관액에 대한 저항성, 장관상피세포에 대한 부착능 및 DPPH radical 소거능이나 환원력과 같은 항산화 활성을 나타내었지만, 이들 활성이 FOS에 의해 증가되진 않았다. 결과적으로 L. acidophilus GK20과 L. paracasei GK74 혼합 배양에 FOS를 첨가한 synbiotic 요구르트는 장내환경 개선과 건강기능 향상에 유용한 것으로 여겨지며, 생리활성은 $4^{\circ}C$에서 7일간 저장 하에서도 일정하게 유지되었음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제30권4호
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• pp.515-525
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• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• 한국수산과학회지
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• 제48권4호
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• pp.466-473
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• 2015

The juvenile red variant of the sea cucumber Stichopus japonicus is distributed worldwide. It is a valuable food source in Korea, China, and Japan. Major issues in farming the juvenile red variant sea cucumber include growth rates and disease resistance. In recent years, studies have focused on feed additives to enhance immune system and health. In this study, we used a common diet (CD), a nutritional diet (ND; mixed nutritional supplements), and an immunity diet (ID; mixed probiotics, spirulina, and levan) and compared the growth rates and immunity of juvenile red variant sea cucumbers fed the experimental diets for 12 weeks. The growth and survival rates in the ID group were significantly increased (P>0.05). This suggests that the feed additives positively influenced immunity and growth in the ID group. However, the immune activity was exhibit a stabilizing effect, and further investigation of immune effects is required.

• Journal of Animal Science and Technology
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• 제46권4호
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• pp.593-602
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• 2004

본 연구는 복합생균제의 급여가 육계의 생산성, 육의 이화학적 특성 및 장내미생물에 미치는 영향을 구명하고 실시하였다. 공시계는 육계초생추 Cobb 480수를 이용하였으며 평사에서 7주간 사용하였다. 시험사료에 첨가된 복합생균제의 첨가수준은 0.1%, 0.1%, 0.4%였으며 기초사료 영양소 함량은 사육전기(0${\sim}$3주)에 ME 3,100kcal/kg, CP 22.0%, 후기(4${\sim}$7주)에는 각각 3,100kcal/kg, 20.0%로 하였다. 복합생균ㄹ제의 급여에 의한 육계의 주령별 체중변화는 4주령까지는 첨가구와 대조가간에 차이를 나타내지 않았는데, 5주령에서는 0.2% 첨가구만 대조구와 유의적인 차이를 보였으나(P<0.05), 6주령에서는 모든 첨가구가 대조구보다 유의적으로 무거웠다.(P<0.05). 사료섭취량은 전기간에서는 통계적인 유의성은 없었으나 6${\sim}$7주령에 0.4% 첨가구가 대조구보다 유의적으로 적게 섭취하였으며(P<0.05), 사료요구율은 4${\sim}$5주령에 0.2% 첨가구가 대조구에 비하여 유의적으로 개선되었으나(P<0.05), 전 시험기간 동안에는 복합생균제의 첨가에 의한 차이를 나타내지 않았다. 7주령시 도체율은 암. 수 전체평균에서 0.4% 첨가구가 무첨가구에 비해 2.80% 증가하였으며(P<0.05), 보강지방축적율은 암.수 전체평균에서 0.2% 첨가구가 무첨가구에 비하여 상당히 개선된 것으로 나타났다. 계육의 가열 감량은 0.4% 첨가구를 제외한 나머지 첨가구가 대조구에 비하여 유의적으로 낮았으며(P<0.05), 전단력은 0.4% 첨가구가 가열 감량과는 반대로 오히려 높았다(P<0.05). 회장과 맹장내의 매생물의 변화는 전체적으로 처리간 통계적 유의성은 보이지 않았으나, 회장과 맹장내의 유산균 수에 있어서는 첨가구가 무첨가구에 비하여 다소 증가되는 경향을 나타내었다. 본 연구의 결과를 종합해 보면, 육계초길부터 급여된 복합생균제가 육계후기에 그 균총이 장내에 정착하게 됨으로 장내 유해세균의 수를 감소시키고, 영양소 흡수에 최적의 조건을 조성하여 생산성 향상을 가져올 수 있을 것으로 사료된다.

• 한국가금학회지
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• 제28권1호
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• pp.41-47
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• 2001

Two experiments were conducted to investigate the effents of feeding single or combined probiltics on performance and intestinal micreflora of broiler chicks for five weeks. Diets based on corn and soybean meal contained 21.50, 19% CP and 3,100, 3,150kcal/kg ME for starting and finishing period, respectively. Lactobacillus salvarius isolated from chicks intestine(LSC), Lactobacillus salvarius isolated from piglet(LSP), Bacillus polyfermenticus(BP) were fed with alone and mixed ones at the level of 0.21 and 0.1% in experiment 1 and 2, respectively. Three hundred eighty four chicks were randomly assigned to eight treatments with four replicates of 12 chicks each per treatment. Weight gain, feed consumption, feed conversion ratio(FCR) were weekly measured for 5 weeks. The number of intestinal micreflora was examined at the end of experiment. In both experiments, weight gain of chicks fed probiltics tended to be similar or higher than control, but was not in LSC+LSP treatment. Feed intake was not consistent among treatments. In experiment 1, FCR of chicks fed LSC alone was the lowest of all treatments, whereas it was significantly higher in LSC+LSP treatments than control(P〈0.05). In experiment 2, LSP supplemental groups tended to increase FCR compared to the control. The number of ileal E. coli was the lowest in LSP alone treatment of experiment 1, whereas cecal E. coli was higher concentration in probiotics supplemental groups than control. Total Lactobacillus of chicks fed probiotics was decreased in ileum, but was no consestency in cecum. In experiment 2, ileal total yeast tended to be higher in probiotics supplemental groups except LSP alone supplement than control. Total Lactobacillus of chicks fed LSC or LSP alone treatments was significantly higher than control(P〈0.05).

• 한국유기농업학회지
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• 제28권3호
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• pp.431-447
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• 2020

This study was conducted to investigate the efficacy of mixed probiotic on the immunity, productivity index and mortality rate in the broiler. Total of 120 one-day-old Ross broilers chicks were randomly assigned into two treatments (control dietary group and probiotic-treated group) with three replications of each treatment. The probiotic group broiler had a lower mortality rate than control during the experimental period. The productivity index in the probiotic group increased significantly than the control group. The weight of the bursa of fabricius was high in the probiotic-treated group than the control group. Activated the immunity level after fed the probiotic mixed diet compared to the control group. Furthermore, the probiotic diet significantly decreased the saturated fatty the control group. Whereas the probiotic mixed diet increased the unsaturated fatty acid than the control group. Afterward, the diet including probiotic induced positive impact on broilers immunity level. This indicates that a probiotic mixed diet could be protecting the intestine from the invasion of a pathogenic organism. It would be beneficial to the poultry industries by decrease the broiler mortality rate with elevated the immunity.

• Asian-Australasian Journal of Animal Sciences
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• 제24권11호
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• pp.1577-1586
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• 2011

This study was carried out to estimate effects of synbiotics containing anaerobic microorganisms and prebiotics on in vitro fermentation characteristics and in situ disappearance rate of fermented total mixed ration (F-TMR). For the in vitro trial, ninety vinyl bags were prepared to analyze temperature, pH, ammonia concentration, microbial growth rate and short chain fatty acid concentration. For the in situ trial, one hundred twenty nylon bags were prepared to analyze dry matter (DM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) disappearance rate. Treatments consisted of a basal diet (US) with prebiotics and probiotics from anaerobic mold (MS), bacteria (BS), yeast (YS) or compound (CS). It was found that temperatures at 14 and 21 days were significantly higher (p<0.05) in the YS and CS than in the others. The pH at 21 days was lower in the CS than in the US. The synbiotic treatments had significantly increased (p<0.05) ammonia concentration at 21 days. The DM disappearance at 72 h was significantly higher (p<0.05) in the MS and CS than in the others. ADF and NDF disappearance rate tended to increase at a rate similar to the DM disappearance rate. Therefore, this study suggests that synbiotics (probiotics with prebiotics) may partially help the quality of fermentation and digestibility of TMR (MS and CS) as fiber disappearance.

• Journal of Dairy Science and Biotechnology
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• 제36권4호
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• pp.220-225
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• 2018

프로바이오틱스의 배양 및 동결건조 과정에서 프로바이오틱스가 사멸하지 않을 정도의 초음파 처리를 통하여 프로바이오틱스의 생존율 및 안정성이 높아지고, 코팅제가 골고루 분산되어 코팅 효율이 높아져 프로바이오틱스의 유통안정성 및 소화 생존율이 개선됨을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제24권10호
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• pp.1438-1444
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• 2014

Lactic acid bacteria (LAB) are probiotics that provide numerous beneficial effects on the host body, especially on the intestine. Combining several strains of LAB, we prepared a formulation containing four different LAB and studied its anti-inflammatory activity both in vitro and in vivo. The formulation significantly reduced NO production from RAW 264.7 cells treated with bacterial lipopolysaccharide, indicating that the formulation might include anti-inflammatory activity. The formulation also suppressed inflammatory change induced by trinitrobenzene sulfonic acid (TNBS) in mice, where oral or rectal administration of the formulation protected the colon tissue from the damage by TNBS. Expressions of the IL-6 and FasL genes appeared to be down-regulated by the formulation in TNBS-treated colon tissues, suggesting that the suppression of those genes may be involved in the anti-inflammatory activity of the formulation.

• International Journal of Oral Biology
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• 제39권1호
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• pp.35-40
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• 2014

Halitosis is caused by consumption of certain foods or drinks and production of volatile sulfur compounds (VSCs) by periodontopathogens. VSCs-related halitosis is not easily removed using mechanical or chemical therapies such as dental floss, plaque control and mouth rinse. Lactobacillus are known to be probiotics and stimulate immune systems of human. Furthermore, L. casei ATCC 334 and L. rhamnosus GG have an effect on protection of dental caries in vitro studies. The aim of this study was to investigate effect of Lactobacillus on halitosis by Fusobacterium nucleatum- and Porphyromonas gingivalis-producing VSCs and to analyze inhibitory mechanism. The periodontopathogens were cultivated in the presence or the absence Lactobacillus, and the level of VSCs was measured by gas chromatograph. For analysis of inhibitory mechanisms, the susceptibility assay of the spent culture medium of Lactobacillus against F. nucleatum and P. gingivalis was investigated. Also, the spent culture medium of Lactobacillus and periodontopathogens were mixed, and the emission of VSCs from the spent culture medium was measured by gas chromatograph. L. casei and L. rhamnosus significantly reduced production of VSCs. L. casei and L. rhamnosus exhibited strong antibacterial activity against F. nucleatum and P. gingivalis. The spent culture medium of L. casei inhibited to emit gaseous hydrogen sulfide, methyl mercaptan and dimethyl sulfide from the spent culture medium of periodontopathogens. However, the spent medium of L. rhamnosus repressed only dimethyl sulfide. L. casei ATCC 334 may improve halitosis by growth inhibition of periodontopathogens and reduction of VSCs emission.