• Preventive Nutrition and Food Science
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• 제6권4호
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• pp.224-229
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• 2001

We investigated the effects of bean sprouts (Glycine max), dropwort (Oenanthe javanica), and radish (Raphanus sativus var. hortensis for. acanthiformis) extracts on alcohol dehydrogenase (ADH). The extracts from three kinds of vegetables were prepared by extracting with boiling water, distilling water, and ethyl alcohol. Among extracts, boiling water extract showed the highest activating effect on ADH, respectively and distilled water extract had a greater effect on ADH activation than that of alcohol extract. The ADH facilitating effect of bean sprout extract by distilled water was significantly higher than dropwort or radish, hut the effect of the bean sprout extract by ethyl alcohol was lower than others. The facilitating effect on ADH of mixture extracts of bean sprout and dropwort were mixed at 1 : 1 mixture of boiled-water extract showed the highest effectiveness. And bean sprout extract separated below 3000 molecular weight (MW) range of extract fraction had greater ADH activity than large MW parts.

• 대한본초학회지
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• 제24권3호
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• pp.51-58
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• 2009

Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of the water and ethanol extracts from pickled soybean products by manufacturing stage. Methods : Yakkong (YK) was pickled for 15 days to prepare Chokong (CK) and Chokong pills (CKP) were mixed Chokong (CK) powder with vinegar. Herbal Chokong pills (HCKP) were made by addition five kinds of medicinal herbs to Chokong. Pickled soybean products by each manufacturing process were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidative and anticancer activities. Results : The highest electron donating abilities (EDA) of the water and ethanol extracts were from HCKP among pickled soybean products and those were 81.2% and 91.5% at 1,000 $\mu$g/mL, respectively. The ethanol extracts of pickled soybean products had higher activities of EDA and superoxide dismutase (SOD) than water extracts. The highest anticancer activity was the water extracts of HCKP against both of MDA and A549 cells. Conclusions : These results suggest that herbal Chokong pills (HCKP) can be used as natural antioxidant to prevent oxidative damage in normal cells probably because of their antioxidative characteristics.

• International Journal of Oral Biology
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• 제32권1호
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• pp.23-34
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• 2007

We performed the present study to investigate whether Rehmannia glutinosa Libosch (RG) extracts (RGX) and Eleutherococcus senticosus Max (ES) extracts (ESX) play any roles in bone metabolism. We examined cellular activities of bone cells by measurement of osteoblastic cell viability, osteoprotegerin (OPG) secretion from osteoblasts, osteoclastogenesis, and osteoclastic activity. There is no cytotoxicity from osteoblasts after treatment with RGX and ESX. The secretion of OPG from the osteoblasts showed marked increases after treatment with RGX and ESX. In addition, RGX and ESX treatment decreased the number of tartrate-resistant acid phosphatase-positive multinucleated cells and the resorption areas. RGX and ESX, when mixed at optimal ratios, induced synergic effects, in vitro. OPB, which showed synergic effects, is the extract of natural ingredients RG and ES mixed at a raw material weight ratio of 4 : 1. It can be suspected that extracts of RG and ES mixtures contains active ingredients involved in bone tissue metabolism and may be effective in improving osteoporosis.

• 한국양봉학회지
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• 제34권4호
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• pp.325-328
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• 2019

We investigated the food storage stability of propolis extracts using homemade meatballs. Propolis extracts were prepared by using the ethanol extraction method. After making the meatballs, propolis was mixed into the meatball materials and egg water with a final concentration of 1% v/v. Microbial cultivation results showed that the cultivation of Staphylococcus aureus, which is one of the food poisoning bacteria, was reduced by more than 90% and the period of storage was 1.5 times longer. Also, it was observed to be effective when propolis was added to meatballs, it was even more effective when propolis was mixed with egg water. Thus, propolis extracts may be a useful ingredient for food storage stability due to its anti-microbacterial function.

• 한국미생물·생명공학회지
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• 제52권1호
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• pp.65-75
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• 2024

The growing demand for functional cosmetics, driven by modern individuals' quest for healthy and aesthetically pleasing skin, is being challenged by reports of side effects and toxicity associated with these products. This underscores the importance of exploring natural plant-based materials for functional cosmetics. This research focuses on the assessment of antioxidant and whitening properties of mixed extracts from Ipomoea nil, Arctostaphylos uva-ursi, and Angelica gigas Nakai, all of which have proven pharmacological benefits. The study evaluated the extracts' total polyphenol and flavonoid contents, ABTS and DPPH radical scavenging activities, SOD-like activity, and xanthine oxidase inhibition to determine their antioxidant capabilities. Moreover, the whitening potential was investigated through tyrosinase activity and melanin production assays, alongside a cytotoxicity evaluation via a cell viability test. The findings revealed that the extracts, IAA-1 to IAA-4, demonstrated both antioxidative and whitening capabilities without exhibiting cytotoxic effects. Notably, the extract IAA-4, processed through ultrasonic and ultrahigh pressure extraction, exhibited superior effectiveness. These results indicate that the cavitation formed during ultrasonic irradiation effectively destroys the plant cell wall by creating high pressure, and as a result, it is judged that useful components are easily extracted. As a result of the study, it was confirmed that the IAA-4 extract could be applied as a material for functional cosmetics.

• 한국식품영양학회지
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• 제13권4호
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• pp.312-318
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• 2000

대두(Glycin max Merrill) 배아의 기능적 활성을 조사하기 위하여 대두를 발아시켜 만든 배아(soybean sprout)을 시료로 하여 0.75% 콜레스테롤 첨가식이로 유발된 고지혈증 Sprague Dawley계 수컷의 흰쥐에 대두 배아의 유기용매 추출물을 급여시킨 후 혈청의 지질 개선효과를 알아보기 위하여 4주간 동안 대두 배아를 섭취시켰다. 식이 급여가 대조군의 체중 및 장기 무게에 미치는 영향은 거의 찾아볼 수 없었으며 혈청 총 콜레스테롤 및 LDL-콜레스테롤의 농도는 대조군에 비하여 낮았고. 콜레스테롤 식이군에 비하여 콜레스테롤 식이와 대두 배아 메탄올 추출물의 혼합투여시 낮게 나타났다. 총 콜레스테롤에 대한 HDL-콜레스테롤의 비는 대두 배아 추출물 급여군이 높게 나타났으며, 동맥경화 지수는 고콜레스테롤 식이군에 비하여 대두 배아 추출물이 낮았다. 또한 혈청중의 유리 콜레스테롤 및 고콜레스테롤 에스테르의 농도는 고콜레스테롤 식이군에 비해 대두 배아의 혼합 급여군이 감소하였고, 인지질의 농도도 콜레스테롤 식이군 보다 콜레스테롤 식이와 대두 배아 급여군이 낮은 경향을 나타내었다.

• Journal of the Korean Wood Science and Technology
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• 제40권2호
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• pp.123-132
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• 2012

Pre-pulping extracts were found to contain a dilute amount of xylo-oligosaccharides and acetic acid as the major components, and many minor components including other organic acids, lignin-derived phenolics, and sugar degradation products. Once separated from the pulp, a secondary hydrolysis step was required to hydrolyze oligomeric hemicellulose sugars into monomeric sugars before fermentation. The following study detailed the extent of hemicellulose recovery by pre-pulping using hot water extraction and characterized the hydrolysis of the extract with respect to comparing acid and enzymatic hydrolysis. The secondaryhydrolysis of hot water extracts made at an H-Factor of 800 was tested for a variety of acid and enzyme loading levels using the sulfuric acid and xylanases. The maximum fermentable sugar yield from acid and enzyme hydrolysis of the extract was 18.7 g/${\ell}$ and 17.7 g/${\ell}$ representing 84.6% and 80.1% of the maximum possible yield, respectively.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.151-151
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• 2003

The ethanol extracts of mixed vegetables (Bioactive V, BV), mixed fruits (Bioactive F, BF) and their liquid formulation (Chungpae Plus(R)) were evaluated for antioxidative and antigenotoxic activity. They were shown to possess the significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and were revealed to show the inhibitory effect of lipid peroxidation as measured by malondialdehyde (MDA) formation.(omitted)

• Journal of Nutrition and Health
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• 제43권1호
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• pp.5-11
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• 2010

본 연구는 명일엽과 그 가공산물의 대사에너지 함량을 분석하기 위해 실시하였다. 명일엽 전초, 명일엽 녹즙, 녹즙박, 발효녹즙 및 녹즙 + 녹즙박 5가지 식품을 기초식이에 30% 첨가하여 실험식이를 제조하였다. 기초식이는 AIN93M을 사용하였다. 실험용 쥐는 실험군당 6마리를 사용하였고, 3일간 실험식이에 적응시킨후 4일간 식이섭취량을 측정하고 분과 뇨를 모두 수거하였다. 수거한 분과 뇨는 bomb calorimeter로 gross energy를 측정하였다. 본 연구결과, 명일엽 가공산물의 대사에너지 함량은 명일엽 1,286.8 kcal/kg, 명일엽 녹즙 1,642.6 kcal/kg, 발효녹즙 2,044.8 kcal/kg, 녹즙박 1,687.5 kacl/kg, 명일엽 녹즙 + 녹즙박 1,763.0 kcal/kg이었다. 수분을 함유한 원물은 각각 193, 82, 102, 270 및 216 kcal/kg이었다. 이를 여러 에너지환산계수를 이용한 에너지가와 비교한 결과 20~60% 차이를 보였다. 특히 원물에서 명일엽은 식품성분표에서는 340 kcal/kg의 열량가를 갖는 것으로 발표되었지만, 동물을 이용한 대사에너지 측정치는 193 kcal/kg으로 76% 낮게 나타났다. 이는 섬유소 함량이 높은 명일엽과 가공 산물들은 실험동물을 이용한 방법이 energy conversion factor로 계산한 수치보다 정확한 대사에너지 함량을 구하기에 더 적합하기 때문으로 판단된다.

• 동아시아식생활학회지
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• 제15권2호
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• pp.182-187
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• 2005

This study was performed to investigate the antimicrobial effect of the Viscum album var. coloratum extracts against food-borne pathogens. First, the Viscum album var. coloratum was extracted with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol, respectively. The antimicrobial activity of the Viscum album var. coloratum extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The petroleum ether extracts of Viscum album var. coloratum showed the highest antimicrobial activity against Bacillus cereus and Shigella dysenteriae. Synergistic effect in inhibition was observed when Viscum album var. coloratum extract was mixed with Perillae folium extract as compared with each extract alone. Finally, the growth inhibition curves were determined by using petroleum ether extracts of Viscum album var. coloratum against Bacillus cereus and Shigella dysenteriae. The petroleum ether extract of Viscum album var. coloratum had strong antimicrobial activity against Bacillus cereus at the concentration of 5,000 ppm. At this concentration, the growth of Bacillus cereus was retarded more than 24 hours and up to 12 hours for Shigella dysenteriae. In conclusion, the petroleum ether extracts of Viscum album var. coloratum inhibit efficiently Bacillus cereus and Shigella dysenteriae.