• Korean Journal of Plant Resources
• /
• v.24 no.5
• /
• pp.628-635
• /
• 2011

This study was conducted to establish the tissue culture system for Korean domestic Miscanthus sinensis, which is used in various purposes such as forage, and bio-energy resources. With the mature seed of Miscanthus, optimum concentrations of plant growth regulators were identified for an efficient callus induction and regeneration. Among the treatments of 1~10 $mg{\cdot}L^{-1}$ 2,4-D, IBA, or NAA, callus induction rate was highest (85.3%) on MS medium containing 5 $mg{\cdot}L^{-1}$ 2,4-D. Under the condition, the callus were efficiently induced and proliferated with comparably lower frequencies of callus browning. In shoot regeneration, the treatment of NAA combined with BAP seemed to contribute more efficient conditions to shoot regeneration than those of NAA with Kinetin or 2-iP. Especially, regeneration efficiency and number of regenerated plants were 83.7% and 5.5 in 3 $mg{\cdot}L^{-1}$ NAA with 5 $mg{\cdot}L^{-1}$ BAP, respectively, which were higher frequencies than those in NAA with Kinetin or 2-iP. In results, 5 $mg{\cdot}L^{-1}$ 2,4-D and 3 $mg{\cdot}L^{-1}$ NAA combined with 5 $mg{\cdot}L^{-1}$ BAP were efficient for embryogenic callus induction and regeneration of Miscanthus. This system would be useful for mass-propagation and developing new cultivars via tissue culture of Miscanthus sinensis.

• Mycobiology
• /
• v.31 no.2
• /
• pp.68-73
• /
• 2003

Roots of Glycine max and Miscanthus sinensis and soil samples were collected from various field sites at Goesan, Chungbuk in Korea. Microscopic observations of the roots indicated high colonization rates of both arbuscular mycorrhizal fungi(AMF) and other fungi. The partial small subunit of ribosomal DNA genes were amplified with the genomic DNA extracted from their roots by nested polymerase chain reaction(PCR) with universal primer NS1 and fungal specific primers AML Restriction fragment length polymorphism(RFLP) was analyzed using the combinations of three restriction enzymes, HinfI, AluI and AsuC21. Nucleotides sequence analysis revealed that ten sequences from Miscanthus sinensis and one sequence from Glycine max were close to those of arbuscular mycorrhizal fungi. Also, 33% of total clones amplified with NS31-AM1 primers from M. sinensis and 97% from G. max were close to Fusarium oxysporum or other pathogenic fungi, and they were successfully distinguished from AME Results suggested that these techniques could help to distinguish arbuscular mycorrhizal fungi from root pathogenic fungi in the plant roots. Especially, DNA amplified by these primers showed distinct polymorphisms between AMF and plant pathogenic species of Fusarium when digested with AsuC21.

• Journal of the Korean Wood Science and Technology
• /
• v.49 no.4
• /
• pp.360-370
• /
• 2021

For the purpose of finding new uses for Miscanthus sinensis var. purpurascens, this study first constructed boards with the particles of the plant and impregnated them with phenolic resin at resin impregnation rates of 30 ± 2%, 40 ± 2%, 50 ± 2%, and 60 ± 2%. The impregnated boards were then carbonized at the carbonization temperature of 800℃, after which their density and mechanical properties were examined according to the different resin impregnation rates. The results showed that density, flexural strength performance, Brinell hardness, and compressive strength increased as the resin impregnation rate increased, thus affecting the physical and mechanical properties of the ceramics made of M. sinensis var. purpurascens particles.

• Journal of Plant Biology
• /
• v.37 no.1
• /
• pp.1-8
• /
• 1994

The effects of fetilization on the structure and function of an early successional Miscanthus sinensis grassland were investigated in an old-field ecosystem at Honjo city, Saitama Prefecture, Japan from 1981 to 1982. Compared to control plot, life form composition of treatment plot was characterized by a decrease of phanerophytes and an increase of hemicryptophytes. Fertilization had a beneficial effect on the growth in aboveground phytomass of M. sinensis, the dominant species. However, it did not cause a change in the total number of stems of M. sinensis. Annual increment of patch diameter of M. sinensis was estimated to be 6-8 cm on an average and there was no significant differecne in the growth rate of patch size between fertilized and nonfertilized plots. To estimate the aboveground phytomass of M. sinensis, several non-destructive parameters were examined. As a result, the patch size showed a high correlation with aboveground phytomass. Thus the patch size was suggested to be most applicable to its estimation. Diversity indices based on phytomass data of component species were increased slightly by fertilizatin, because relative dominance of some other species, especially of Artemisia princeps increased, while that of M. sinensis decreased. Fertilizer application resulted in a decrease in the total number of species.

• Journal of the Korean Wood Science and Technology
• /
• v.42 no.6
• /
• pp.723-731
• /
• 2014

Nondestructive evaluation (NDE) technique method using a resonance frequency mode was carried out for ceramics made by different carbonizing temperatures (600, 800, 1000, $1200^{\circ}C$) after impregnating the phenol resin with Miscanthus sinensis var. purpurascen particle boards. Dynamic modulus of elasticity increased with increasing carbonizing temperature. There were a close relationship of dynamic modulus of elasticity and static bending modulus of elasticity to modulus of rupture (MOR). However, the result indicated that correlation coefficient was higher in dynamic modulus of elasticity to MOR than that in static modulus of elasticity to MOR. Therefore, the dynamic modulus of elasticity using resonance frequency by free vibration mode is more useful as a nondestructive evaluation method for predicting the MOR of ceramics made by different carbonizing temperature for Miscanthus sinensis var. purpurascens particle boards.

• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.47 no.6
• /
• pp.89-97
• /
• 2015

In this study, dissolution characteristics of 60% LiBr aqueous solution for Miscanthus sinensis holocellulose in accordance with heating time and characteristics of regenerated films were analyzed. Miscanthus sinensis holocellulose was made by peracetic acid method. During the dissolution of 60% LiBr solution for the holocellulose, the dissolution was started from the tip of the cellulose fiber after about 7 minutes, and proceeded as it swollen like a balloon. A lot of Si was identified by analyzing hollocellulose regenerated film through SEM/EDS. Cross section of regenerated film as dissolution time till 40 minutes of dissolution showed multilayered structure and fiber orientation. But after 40 minutes, multilayered structure and fiber orientation was not observed. The crystal structure of the holecellulose was transformed cellulose I into cellulose II. Therefore, dissolution for 20 minutes with 60% LiBr solution in the condition of $190^{\circ}C$ hot plate was shown as an optimum condition to manufacture the holocellulose regenerated film.

• Asian Journal of Turfgrass Science
• /
• v.9 no.4
• /
• pp.275-284
• /
• 1995

In this investigation, the accumulation mineralization and annual cycle of organic P has been studied in grassland ecosysterns of a Z japonica grassland and a M sinensis grassland on Mt. Kwanak. The basic models of the accumulation and mineralization for ash components of a grass-litter have been presented as the equations (1), (2), and (3). The equations (7)~(10) for organic P are derived from these basic concepts. There was a highty significant relationship between organic matter and organic P. The estimates between organic matter and organic P correlated very high significance. The parameter factors k or k' of mineralization of organic P for the Z. japonica and M sinensis asslands were k=0.412 or k'=0.292 and k=0.224 or k'=0.183, respectively. The time required for a cycle to be completed from organic P to inorganic P of 50, 95 and 99 % are 3.9, 16.7 and 27.8 years in the Z. japonica grassland and 4.1, 17.7 and 29.4 years in the M sinensis grassland. The annual P cycle formulae for mineralization were based on the equations (5), (11) and (12). Annual yields of mineralization for organic P in the steady state grasslands of Z. japonica and M sinensis were 0.407 and 0.504g /$m^2$, respectively.

• Asian Journal of Turfgrass Science
• /
• v.11 no.1
• /
• pp.1-7
• /
• 1997

This study was performed to find out the mineral cycles of manganese and zinc in dynamic grassland ecosystems in a steady state condition at the northwest side on Mt. Kwanak. The experimental results may be summarized on the communities of a Zoysia japonica and a Miscanthus sincnsis as follows. As compared with some properties of the surface soils among two semi-natural grasslands, manganese and zinc was greater quantity in a Miscanthus sincnsis than in a Zoysia japonica on Mt. Kwana For the case of steady procluction and release, the ratio of annual mineral production to the amount accumulated on the top of mineral soil in a steady state provides the estimates of release constant k. The release constants of Mn and Zn of the litter were 0.19 and 0. 14 in the Zoysia japon- ica grassland, and were 0.44 and 0.41 in a Miscanthus sincnsis grassland, respectively. The halftimes of Mn and Zn required for the release or accumulation of the litter on the grassland were 3. 65 and 4.95 years in the Zoysia japonica, and were 1.57 and 1.69 years in the Miscanthus sincnsis, respectively. The amounts of annual cycles for Mn and Zn in the grassland ecosystem under the steady-state conditions were 58.60 and 21.46 mg /$m^2$ in a Zoysia japonica, and were 372.12 and 321.49 ing /m$^2$ in a Miscanthus sincnsis grassland. Key words: Zoysia japonica. Miscanthus sincnsis, Mt. Kwanak, Manganese, Zinc, Mineral cycles.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.1 no.1
• /
• pp.54-62
• /
• 1998

This study measured the shearing resistance of the roots of the Sasamorpha purpurascens, Miscanthus sinensis, Lespedeza cyrtobotrya by the tensile strength gained through their individual tensile test for the Root Reinforcement Model. The results to have measured this stress by experiment are as follows. 1) The mean root diameter of the Lespedeza cyrtobotrya used for this experiment was 2.19mm and the mean tensile stress was calculated as $929.489kgf/cm^2$. As for the Sasamorpha purpurascens, its mean root diameter was 1.727mm, and the mean tensile stress was $292.069kgf/cm^2$. And as for the Miscanthus sinensis, its mean root diameter was 0.814mm, and the mean tensile stress was $696.947kgf/cm^2$. And so, it was grasped that Lespedeza cyrtobotrya was highest in tensile stress. 2) ${\Delta}Cr(kg/cm^2)$ of the shearing resistance calculated by estimating the areal ratio of roots at $10^{-3}$ is $1.069kg/cm^2$ in Lespedeza cyrtobotrya, $0.336kg/cm^2$ in Sasamorpha purpurascens, and $0.801kg/cm^2$ in Miscanthus sinensis. That is, Lespedeza cyrtobotrya has the highest shearing resistance. However, since a precise analysis of the controlled factors of the slope analyses are demanded for more accurate dynamic analyses, the future demands a study on this.

• Journal of the Korean Wood Science and Technology
• /
• v.42 no.3
• /
• pp.251-257
• /
• 2014

For new use development of miscanthus sinensis var. purpurascens, which investigated properties of ceramics made from different carbonization temperature ($600{\sim}1,200^{\circ}C$) and percentage of resin impregnation(30~60%) using a mincanthus board. As the carbonization temperature increased, the density, thickness shrinkage, linear shrinkage, weight loss were increased. As the percentage of resin impregnation increased, the density, linear shrinkage and weight loss were increased. On the other hand, thickness shrinkage decreased.