• 한국자원식물학회지
• /
• 제24권5호
• /
• pp.628-635
• /
• 2011

본 연구는 최근 한방자원, 사료자원, 바이오에너지 자원 등 다양하게 이용되는 국내 자생 억새(Miscanthus sinensis)의 대량생산 및 신품종 개발을 위한 조직배양체계 확립을 위해 수행되었다. 이를 위해 억새 완숙종자로부터의 캘러스 유도와 재분화를 위한 식물생장조절제의 적정농도를 규명하였다. 억새의 성숙종자유래 배발생 캘러스 유도를 위해 2,4-D, IBA, NAA를 1~10 $mg{\cdot}L^{-1}$의 농도로 단용 처리한 결과, 5 $mg{\cdot}L^{-1}$ 2,4-D 처리에서 가장 높은 85.3%의 캘러스 유도율과 캘러스의 증식을 보였으며 조직배양 과정 중 갈변화율도 가장 낮았다. 또한, 캘러스의 재분화를 위해 옥신인 NAA와 Kinetin, 2-iP, 또는 BAP 등의 사이토키닌을 혼용 처리한 결과, 각각 19.0%~59.0%, 23.0%~67.3%, 14.7%~83.7%의 재분화율을 보여 NAA와 BAP의 혼용 처리구가 NAA와 Kinetin 또는 2-iP와 혼용 처리구보다 식물체 재분화에 효과적이었다. 특히 3 $mg{\cdot}L^{-1}$ NAA와 5 $mg{\cdot}L^{-1}$ BAP 혼용 처리된 배지에서의 재분화율이 83.7%로 가장 높게 나타났으며, 캘러스 당 재분화 식물체 개수도 5.5개로 동일농도의 2-iP 또는 Kinetin 혼용 처리 시 2.1 및 2.0개보다 많았다. 본 연구결과 억새 성숙 종자로부터의 배발생 캘러스 유도에는 5 $mg{\cdot}L^{-1}$ 2,4-D가 그리고 캘러스의 재분화에는 3 $mg{\cdot}L^{-1}$ NAA와 5 $mg{\cdot}L^{-1}$ BAP 혼용 처리가 가장 효율적이었다. 본 연구를 통해 확립된 조직배양체계는 억새의 대량생산 및 신품종 개발에 유용할 것으로 판단된다.

• Mycobiology
• /
• 제31권2호
• /
• pp.68-73
• /
• 2003

Roots of Glycine max and Miscanthus sinensis and soil samples were collected from various field sites at Goesan, Chungbuk in Korea. Microscopic observations of the roots indicated high colonization rates of both arbuscular mycorrhizal fungi(AMF) and other fungi. The partial small subunit of ribosomal DNA genes were amplified with the genomic DNA extracted from their roots by nested polymerase chain reaction(PCR) with universal primer NS1 and fungal specific primers AML Restriction fragment length polymorphism(RFLP) was analyzed using the combinations of three restriction enzymes, HinfI, AluI and AsuC21. Nucleotides sequence analysis revealed that ten sequences from Miscanthus sinensis and one sequence from Glycine max were close to those of arbuscular mycorrhizal fungi. Also, 33% of total clones amplified with NS31-AM1 primers from M. sinensis and 97% from G. max were close to Fusarium oxysporum or other pathogenic fungi, and they were successfully distinguished from AME Results suggested that these techniques could help to distinguish arbuscular mycorrhizal fungi from root pathogenic fungi in the plant roots. Especially, DNA amplified by these primers showed distinct polymorphisms between AMF and plant pathogenic species of Fusarium when digested with AsuC21.

• Journal of the Korean Wood Science and Technology
• /
• 제49권4호
• /
• pp.360-370
• /
• 2021

본 연구는 거대억새의 활용방안을 찾기 위하여 거대억새 파티클로 보드를 제조한 다음 페놀수지를 함침한 후 수지함침 율이 30 ± 2%, 40 ± 2%, 50 ± 2%, 60 ± 2% 인 함침보드를 탄화온도 800℃로 탄화하여 수지함침율에 따라 밀도 및 역학적 성질을 조사하였다. 밀도, 휨강도성능, Brinell경도 및 압축강도는 수지함침율이 증가 할수록 증가하여 거대억새 파티클로 제조된 세라믹의 물리 및 역학적 성질에 영향을 주었다.

• Journal of Plant Biology
• /
• 제37권1호
• /
• pp.1-8
• /
• 1994

The effects of fetilization on the structure and function of an early successional Miscanthus sinensis grassland were investigated in an old-field ecosystem at Honjo city, Saitama Prefecture, Japan from 1981 to 1982. Compared to control plot, life form composition of treatment plot was characterized by a decrease of phanerophytes and an increase of hemicryptophytes. Fertilization had a beneficial effect on the growth in aboveground phytomass of M. sinensis, the dominant species. However, it did not cause a change in the total number of stems of M. sinensis. Annual increment of patch diameter of M. sinensis was estimated to be 6-8 cm on an average and there was no significant differecne in the growth rate of patch size between fertilized and nonfertilized plots. To estimate the aboveground phytomass of M. sinensis, several non-destructive parameters were examined. As a result, the patch size showed a high correlation with aboveground phytomass. Thus the patch size was suggested to be most applicable to its estimation. Diversity indices based on phytomass data of component species were increased slightly by fertilizatin, because relative dominance of some other species, especially of Artemisia princeps increased, while that of M. sinensis decreased. Fertilizer application resulted in a decrease in the total number of species.

• Journal of the Korean Wood Science and Technology
• /
• 제42권6호
• /
• pp.723-731
• /
• 2014

공진주파수 모드를 이용하는 비파괴 평가기술법을 거대억새 파티클 보드를 페놀수지로 함침한 후 탄화온도별(600, 800, 1000, $1200^{\circ}C$)로 제조한 세라믹에 적용하였다. 동적 탄성계수와 정적 휨 탄성계수는 탄화온도가 증가할수록 증가하였다. 휨 강도에 대한 동적 탄성계수 및 정적 휨 탄성계수는 밀접한 상관관계를 나타내었다. 그러나 휨 강도에 대한 상관관계에서 정적 탄성계수 보다 동적 탄성계수가 더 밀접한 상관관계를 나타내었다. 따라서 공진 주파수 모드를 사용하는 동적 탄성계수측정법으로 소성온도에 따라 제조된 거대억대 파티클보드의 휨 강도를 예측하는 비파괴 평가 방법으로 유용할 것으로 판단된다.

• 펄프종이기술
• /
• 제47권6호
• /
• pp.89-97
• /
• 2015

In this study, dissolution characteristics of 60% LiBr aqueous solution for Miscanthus sinensis holocellulose in accordance with heating time and characteristics of regenerated films were analyzed. Miscanthus sinensis holocellulose was made by peracetic acid method. During the dissolution of 60% LiBr solution for the holocellulose, the dissolution was started from the tip of the cellulose fiber after about 7 minutes, and proceeded as it swollen like a balloon. A lot of Si was identified by analyzing hollocellulose regenerated film through SEM/EDS. Cross section of regenerated film as dissolution time till 40 minutes of dissolution showed multilayered structure and fiber orientation. But after 40 minutes, multilayered structure and fiber orientation was not observed. The crystal structure of the holecellulose was transformed cellulose I into cellulose II. Therefore, dissolution for 20 minutes with 60% LiBr solution in the condition of $190^{\circ}C$ hot plate was shown as an optimum condition to manufacture the holocellulose regenerated film.

• 아시안잔디학회지
• /
• 제9권4호
• /
• pp.275-284
• /
• 1995

In this investigation, the accumulation mineralization and annual cycle of organic P has been studied in grassland ecosysterns of a Z japonica grassland and a M sinensis grassland on Mt. Kwanak. The basic models of the accumulation and mineralization for ash components of a grass-litter have been presented as the equations (1), (2), and (3). The equations (7)~(10) for organic P are derived from these basic concepts. There was a highty significant relationship between organic matter and organic P. The estimates between organic matter and organic P correlated very high significance. The parameter factors k or k' of mineralization of organic P for the Z. japonica and M sinensis asslands were k=0.412 or k'=0.292 and k=0.224 or k'=0.183, respectively. The time required for a cycle to be completed from organic P to inorganic P of 50, 95 and 99 % are 3.9, 16.7 and 27.8 years in the Z. japonica grassland and 4.1, 17.7 and 29.4 years in the M sinensis grassland. The annual P cycle formulae for mineralization were based on the equations (5), (11) and (12). Annual yields of mineralization for organic P in the steady state grasslands of Z. japonica and M sinensis were 0.407 and 0.504g /$m^2$, respectively.

• 아시안잔디학회지
• /
• 제11권1호
• /
• pp.1-7
• /
• 1997

This study was performed to find out the mineral cycles of manganese and zinc in dynamic grassland ecosystems in a steady state condition at the northwest side on Mt. Kwanak. The experimental results may be summarized on the communities of a Zoysia japonica and a Miscanthus sincnsis as follows. As compared with some properties of the surface soils among two semi-natural grasslands, manganese and zinc was greater quantity in a Miscanthus sincnsis than in a Zoysia japonica on Mt. Kwana For the case of steady procluction and release, the ratio of annual mineral production to the amount accumulated on the top of mineral soil in a steady state provides the estimates of release constant k. The release constants of Mn and Zn of the litter were 0.19 and 0. 14 in the Zoysia japon- ica grassland, and were 0.44 and 0.41 in a Miscanthus sincnsis grassland, respectively. The halftimes of Mn and Zn required for the release or accumulation of the litter on the grassland were 3. 65 and 4.95 years in the Zoysia japonica, and were 1.57 and 1.69 years in the Miscanthus sincnsis, respectively. The amounts of annual cycles for Mn and Zn in the grassland ecosystem under the steady-state conditions were 58.60 and 21.46 mg /$m^2$ in a Zoysia japonica, and were 372.12 and 321.49 ing /m$^2$ in a Miscanthus sincnsis grassland. Key words: Zoysia japonica. Miscanthus sincnsis, Mt. Kwanak, Manganese, Zinc, Mineral cycles.

• 한국환경복원기술학회지
• /
• 제1권1호
• /
• pp.54-62
• /
• 1998

This study measured the shearing resistance of the roots of the Sasamorpha purpurascens, Miscanthus sinensis, Lespedeza cyrtobotrya by the tensile strength gained through their individual tensile test for the Root Reinforcement Model. The results to have measured this stress by experiment are as follows. 1) The mean root diameter of the Lespedeza cyrtobotrya used for this experiment was 2.19mm and the mean tensile stress was calculated as $929.489kgf/cm^2$. As for the Sasamorpha purpurascens, its mean root diameter was 1.727mm, and the mean tensile stress was $292.069kgf/cm^2$. And as for the Miscanthus sinensis, its mean root diameter was 0.814mm, and the mean tensile stress was $696.947kgf/cm^2$. And so, it was grasped that Lespedeza cyrtobotrya was highest in tensile stress. 2) ${\Delta}Cr(kg/cm^2)$ of the shearing resistance calculated by estimating the areal ratio of roots at $10^{-3}$ is $1.069kg/cm^2$ in Lespedeza cyrtobotrya, $0.336kg/cm^2$ in Sasamorpha purpurascens, and $0.801kg/cm^2$ in Miscanthus sinensis. That is, Lespedeza cyrtobotrya has the highest shearing resistance. However, since a precise analysis of the controlled factors of the slope analyses are demanded for more accurate dynamic analyses, the future demands a study on this.

• Journal of the Korean Wood Science and Technology
• /
• 제42권3호
• /
• pp.251-257
• /
• 2014

거대억새의 활용방안을 찾기 위하여 거대억새 파티클로 보드를 제조한 후 수지함침율 50%일 때 탄화온도 $600{\sim}1,200^{\circ}C$별로, 탄화온도 $800^{\circ}C$일 때 수지함침율 30~60%별로 탄화하여 세라믹을 제조한 후 물성을 조사하였다. 탄화온도가 증가함에 따라 탄화 후 밀도, 두께감소율, 길이감소율 및 중량감소율도 증가하였다. 수지함침율이 증가함에 따라 탄화 후 밀도, 길이감소율 및 중량 감소율은 증가하였으나 두께감소율은 감소하였다.