• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.429-440
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• 2019

Several studies have attempted to identify factors associated with longevity and maintenance of health in centenarians. In this study, we analyzed and compared the gut microbiota of centenarians in longevity villages with the elderly and adults in the same region and urbanized towns. Fecal samples were collected from centenarians, elderly, and young adults in longevity villages, and the gut microbiota sequences of elderly and young adults in urbanized towns of Korea were obtained from public databases. The relative abundance of Firmicutes was found to be considerably higher in subjects from longevity villages than those from urbanized towns, whereas Bacteroidetes was lower. Age-related rearrangement of gut microbiota was observed in centenarians, such as reduced proportions of Faecalibacterium and Prevotella, and increased proportion of Escherichia, along with higher abundances of Akkermansia, Clostridium, Collinsella, and uncultured Christensenellaceae. Gut microbiota of centenarians in rehabilitation hospitals were also different to those residing at home. These differences could be due to differences in diet patterns and living environments. In addition, phosphatidylinositol signaling system, glycosphingolipid biosynthesis, and various types of N-glycan biosynthesis were predicted to be higher in the gut microbiota of centenarians (corrected p < 0.05). These three metabolic pathways of gut microbiota can be associated with the immune status and healthy gut environment of centenarians. Although further studies are necessary to validate the function of microbiota between groups, this study provides valuable information on centenarians' gut microbiota.

• 천문학회보
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• 제43권2호
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• pp.39.2-39.2
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• 2018

RR Tel is an interacting binary system in which a hot white dwarf (WD) accretes matter from a Mira variable via gravitational capture of the stellar wind. We present a high-resolution optical spectrum of RR Tel obtained with MIKE at Magellan-Clay telescope, Chile. We find broad emission features at 6825, 7082, 7023, and $7053{\AA}$, which are formed through Raman scattering of far-UV O VI ${\lambda}{\lambda}$ 1032 and $1038{\AA}$, C II ${\lambda}{\lambda}$ 1036 and $1037{\AA}$ with atomic hydrogen. Raman O VI 6825 and 7082 features are characterized by double-peaked profiles indicative of an accretion flow with a characteristic speed ~ 30km/s, whereas the Raman C II features exhibit a single Gaussian profile with FWHM ${\sim}10{\AA}$. Monte Carlo simulations for Raman O VI and C II are performed by assuming that the emission nebula around the WD consists of the inner O VI disk with a representative scale of 1 AU and the outer part with C II sphere. The best fit for Raman profiles is obtained with an asymmetric matter distribution of the O VI disk, the mass loss rate of the cool companion ${\dot{M}}{\sim}2{\times}10^{-6}M_{{\odot}/yr}$ and the wind terminal velocity v~10 km/s. We also find O VI doublet at 3811 and $3834{\AA}$, which are blended with other emission lines. Our profile decomposition shows that the O VI ${\lambda}{\lambda}$ 3811, 3834 doublet have a single Gaussian profile with a width ~ 25 km/s. A comparison of the restored fluxes of C II ${\lambda}{\lambda}$ 1036 and 1037 from Raman C II features with the observed C II ${\lambda}1335$ leads to an estimate of a lower bound of N(CII) > $9.87{\times}10^{13}cm^{-2}$ toward RR Tel, which appears consistent with the presumed distance D ~ 2.6 kpc.

• 융합정보논문지
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• 제10권12호
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• pp.58-66
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• 2020

본 연구는 간호대학생의 좋은죽음인식, 호스피스인식, 임종간호태도의 정도와 세 변수간의 관계를 알아보고 임종간호태도에 미치는 영향 요인을 규명하여 간호대학생의 죽음에 대한 인식과 호스피스 간호, 임종간호 교육의 방향 설정에 기여하고자 설계된 조사연구이다. 대상자는 D시 소재 대학교 간호학과 재학생으로 임상실습에 참여한 경험이 있는 3, 4학년 학생 중 연구목적을 이해하고 자발적으로 연구에 참여한 대상자에게 2019년 5-6월 설문지를 배부하고 229부를 최종 분석하였다. 연구결과, 간호대학생의 좋은죽음인식, 호스피스인식, 임종간호태도 세 변수 간에는 통계적으로 유의미한 순상관관계가 있었다. 간호대학생의 임종간호태도에 영향을 미치는 요인을 분석한 결과, 임종간호태도에 가장 큰 영향력을 미치는 변수는 호스피스인식이었으며, 호스피스인식과 좋은죽음인식의 회귀모형의 설명력은 39.3%였다. 따라서 예비 간호사인 간호대학생이 긍정적인 죽음인식과 임종간호태도를 갖게 하기 위해서는 호스피스와 좋은죽음에 대한 교육이 가능하도록 교육과정을 정비하는 노력이 필요할 것으로 사료된다.

• 한국간호교육학회지
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• 제27권3호
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• pp.321-332
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• 2021

Purpose: The purpose of this study is to determine the influence of the learning environment on nursing students' clinical practice education and the violence experienced during clinical practice on vocational identity. Methods: The design of the study was a descriptive survey, and data were collected from November 15 to November 27, 2019. The data of the study were obtained from 515 nursing students attending three universities using self-administered questionnaires. Data were analyzed using the SPSS 25.0 program. Results: For the experience of violence, verbal violence (98.3%) was the type most commonly experienced, and patients (97.7%) were the most frequent perpetrators. The clinical learning environment was perceived differently according to gender, personality, interpersonal relationship, satisfaction with nursing, clinical practice satisfaction, violence prevention education, the need for violence prevention education, sexual violence experiences, and violent perpetrators. The most influential factor on vocational identity was satisfaction with the nursing major (β=0.24, p<.001), followed by extroverted personality (β=0.18, p<.001), clinical learning environment (β=0.15, p=.001), satisfaction with clinical practice (β=0.15, p=.002), and the experience of violence by patients (β=-0.10, p=.016), which together explained 24.1% of the variance in the model. Conclusion: It is necessary to make efforts to ensure that students do not experience violence during clinical practice, to maintain a close cooperative relationship between university and clinical institutions to improve the learning environment for clinical practice, and to make the clinical field an educational learning environment.

• 한국수소및신에너지학회논문집
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• 제33권5호
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• pp.470-498
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• 2022

Recently, the world has been considering hydrogen energy as the primary energy transition means to achieve carbon neutrality by 2050. In order to achieve the goal of reducing greenhouse gas emissions, Korea is also promoting a clean hydrogen economy. However, it is necessary to introduce various clean hydrogen from overseas so that the projected demand can not meet the domestically produced. For this study, we conducted the policy comparison approach between countries other than the generally considered technical and economic approaches. The finding proposes the direction of bilateral cooperation for a strategy of securing overseas clean hydrogen from a geopolitical perspective. Germany was a target country for the policy comparison since it has a high proportion of manufacturing, like Korea, and is taking the lead in the renewable-based energy transition policy. According to the survey and analysis of the policy establishment status and new projects of the two countries, Germany is promoting bilateral international cooperation in the hydrogen area with about 33 countries based on 7 types of activities. In comparison, Korea is involved in bilateral cooperation with about 12 countries on relatively few activities. Among the types of bilateral cooperation, R&D cooperation with advanced countries for hydrogen technology was a common activity type. Germany preemptively promotes cooperation for demonstration and commercialization, considering geopolitical means and strengthening manpower training and assistance on policy and regulation to preoccupy the market for the future. Therefore, it is necessary to consider establishing a network of an entire life cycle of supply and demand network that links the future market with securing clean hydrogen considering the geopolitical distribution. To this end, Korea also needs to expand bilateral cooperation countries by activity type, and it seems necessary to seek various geopolitical-based bilateral cooperation and support measures for developing countries to diversify the supply sources of hydrogen.

• 이베로아메리카
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• 제12권1호
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• pp.187-212
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• 2010

El cine como medio de narrar el trauma histórico de los cubanos se formó especialmente en los años sesenta gracias al Instituto Cubano del Arte e Industria Cinematográficos y se sitúa en la cumbre. En el cine hecho por el director cubano Humberto Solás el discurso de historia, trauma y su memoria tiende a relatar básicamente sobre el mensaje de no olvidar el trauma histórico a través de buscar la manera eficaz e intrínseca de narrarlo. El hecho de no olvidar el trauma histórico y de poder enfrentarse con los elementos causantes del trauma significa el poder de tener la posibilidad probable de adquirir la mirada objetiva para observar la experiencia 'histórica' y la memoria 'cotidiana', y sus relaciones. Si consideramos el hecho de que la tendencia de la víctima suele querer olvidar la memoria del trauma por el miedo y dolor de enfrentarse con la representación del trauma, el enfrentarse con el trauma representado ya puede significar que la víctima esté en un trámite de conseguir la penetración con la cual supera lo negativo que le ofrece el trauma a la víctima. Este artículo se dedica especialmente para averiguar la estrategia del director cubano para realizar este fin de encontrar la manera adecuada en narrar el trauma cubano para que los cubanos puedan adquirir la penetración a través de la identificación y la simpatía entre los personajes del cine y los espectadores, enfocando con detalle en los carácteres de los protagonistas femeninas que son tres diferentes individuos en el mismo número de episodios. Cada protagonista, Lucía en cada episodio experimenta el trauma personal con diversos motivos, pero a pesar del dolor y del miedo del trauma histórico se despiertan los espectadores, quienes se identifican con la protagonista a través de la simpatía y la identificación, llegándose al momento de conseguir la penetración con la cual mira el futuro de la sociedad cubana con el objetivismo libre de los traumas históricos.

• The Korean Journal of Physiology and Pharmacology
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• 제27권4호
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• pp.407-416
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• 2023

The regeneration of myocardium following acute circulatory events remains a challenge, despite numerous efforts. Mesenchymal stem cells (MSCs) present a promising cell therapy option, but their differentiation into cardiomyocytes is a time-consuming process. Although it has been demonstrated that PSME4 degrades acetyl-YAP1, the role of PSME4 in the cardiac commitment of MSCs has not been fully elucidated. Here we reported the novel role of PSME4 in MSCs cardiac commitment. It was found that overnight treatment with apicidin in primary-cultured mouse MSCs led to rapid cardiac commitment, while MSCs from PSME4 knock-out mice did not undergo this process. Cardiac commitment was also observed using lentivirus-mediated PSME4 knockdown in immortalized human MSCs. Immunofluorescence and Western blot experiments revealed that YAP1 persisted in the nucleus of PSME4 knockdown cells even after apicidin treatment. To investigate the importance of YAP1 removal, MSCs were treated with shYAP1 and apicidin simultaneously. This combined treatment resulted in rapid YAP1 elimination and accelerated cardiac commitment. However, overexpression of acetylation-resistant YAP1 in apicidin-treated MSCs impeded cardiac commitment. In addition to apicidin, the universal effect of histone deacetylase (HDAC) inhibition on cardiac commitment was confirmed using tubastatin A and HDAC6 siRNA. Collectively, this study demonstrates that PSME4 is crucial for promoting the cardiac commitment of MSCs. HDAC inhibition acetylates YAP1 and facilitates its translocation to the nucleus, where it is removed by PSME4, promoting cardiac commitment. The failure of YAP1 to translocate or be eliminated from the nucleus results in the MSCs' inability to undergo cardiac commitment.

• Journal of Gastric Cancer
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• 제23권4호
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• pp.549-560
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• 2023

Purpose: According to the American Joint Committee on Cancer cancer staging system, positive peritoneal washing cytology (PWC) indicates stage IV gastric cancer. However, rapid intraoperative diagnosis of PWC has no established reliable method. This study evaluated and compared the diagnostic accuracy of the Shorr and the modified ultrafast Papanicolaou (MUFP) methods for intraoperative PWC. Materials and Methods: This study included patients with gastric cancer who were clinically diagnosed with stage cT3 or higher. The Shorr and MUFP methods were performed on all PWC specimens, and the results were compared with those of conventional Papanicolaou (PAP) staining with carcinoembryonic antigen immunohistochemistry. Sensitivity, specificity, and partial likelihood tests were used to compare the 2 methods. Results: Forty patients underwent intraoperative PWC between November 2019 and August 2021. The average time between specimen reception and slide preparation using Shorr and MUFP methods was 44.4±4.5 minutes, and the average time between specimen reception and pathologic diagnosis was 53.9±8.9 minutes. Eight patients (20.0%) had positive cytology in PAP staining. The Shorr method had a sensitivity of 75.0% and specificity of 93.8%; the MUFP method had 62.5% sensitivity and 100.0% specificity. The area under the curve was 0.844 for Shorr and 0.813 for MUFP. In comparing the C-indices of each method with overall survival, no difference was found among the Shorr, MUFP, and conventional PAP methods. Conclusions: The Shorr and MUFP methods are acceptable for the intraoperative diagnosis of PWC in advanced gastric cancer.

• Journal of Gastric Cancer
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• 제23권4호
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• pp.561-573
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• 2023

Purpose: This study aimed to compare the long-term functional and patient-reported outcomes between intra-corporeal delta-shaped gastroduodenostomy and gastrojejunostomy after laparoscopic distal gastrectomy for gastric cancer. Materials and Methods: We retrospectively reviewed clinicopathological data from 616 patients who had undergone laparoscopic distal gastrectomy for stage I gastric cancer between January 2015 and September 2020. Among them, 232 patients who had undergone delta-shaped anastomosis and another 232 who had undergone Billroth II anastomosis were matched using propensity scores. Confounding variables included age, sex, body mass index, physical status classification, tumor location, and T classification. Postoperative complications, nutritional outcomes, endoscopic findings, and quality of life (QoL) were compared between the 2 groups. Results: No significant differences in postoperative complications or nutritional parameters between the two groups were observed. Annual endoscopic findings revealed more residual food and less bile reflux in the delta group (P<0.001) than in the Billroth II group. Changes of QoL were significantly different regarding emotional function, insomnia, diarrhea, reflux symptoms, and dry mouth (P=0.007, P=0.002, P=0.013, P=0.001, and P=0.03, respectively). Among them, the delta group had worse insomnia, reflux symptoms, and dry mouth within three months postoperatively. Conclusions: Long-term nutritional outcomes and QoL were comparable between the delta and Billroth II groups. However, more residual food and worse short-term QoL regarding insomnia, reflux symptoms, and dry mouth were observed in the delta group. Longer fasting time before endoscopic evaluation and short-term symptom management would have been helpful for the delta group.

• 한국수학교육학회지시리즈C:초등수학교육
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• 제26권4호
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• pp.349-368
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• 2023

본 연구의 목적은 수학적 모델링 수업에서 학생들이 개념적 지식과 절차적 지식을 연결하는 방안을 탐색하는 것이다. 이에, 초등학생들이 개념적 지식과 절차적 지식을 연결하는 데 어려움을 보이는 학습 내용 중 최대공약수를 선정하고. 개념적 지식과 절차적 지식을 연결하면서 최대공약수 관련 문제를 해결할 수 있도록 수학적 모델링 수업을 설계하여 실행하였다. 분석 결과, 해당 수학적 모델링 수업은 학생들이 개념적 지식과 절차적 지식을 연결하여 문제를 해결하는 데 긍정적인 영향을 미친 것으로 나타났다. 또한 실제 수업 적용을 통해 수학적 모델링 수업에서 개념적 지식과 절차적 지식을 의미 있게 연결하기 위한 교수학습 방안을 도출하였다.