• 제목/요약/키워드: Minnesota

검색결과 633건 처리시간 0.027초

Socialization and Teen Magazines: What are the Messages?

  • Kim, K.P. Johnson;Mun, Jung-Mee;Ju, Hae-Won;Kang, Ju-Young M.;Kim, Hye-Young;Wu, Juanjuan
    • International Journal of Costume and Fashion
    • /
    • 제11권2호
    • /
    • pp.1-12
    • /
    • 2011
  • As fashion magazines are important socialization influences, our purpose was to examine the content of articles in two teen magazines: one with a long publication history (Seventeen) and one relatively new market entry (Teen Vogue). We addressed the following questions: (1) What are the patterns of content of the feature articles? (2) How frequently is this content related to appearance management or fashion consumption? and (3) What, if any, differences exist in contents between the traditional teen magazine and the new market entry? A content analysis of 1,191 articles published during 2008 and 2009 revealed the largest percentage of content in both magazines was fashion. Other than the topic of fashion, Seventeen concentrated on teen life issues whereas Teen Vogue focused on celebrities. Understanding these are fashion publications, we suggest there are opportunities for both magazines to allocate further attention to other issues in the lives of teens in addition to beauty and consumption.

Bubble size characteristics in the wake of ventilated hydrofoils with two aeration configurations

  • Karn, Ashish;Ellis, Christopher R;Milliren, Christopher;Hong, Jiarong;Scott, David;Arndt, Roger EA;Gulliver, John S
    • International Journal of Fluid Machinery and Systems
    • /
    • 제8권2호
    • /
    • pp.73-84
    • /
    • 2015
  • Aerating hydroturbines have recently been proposed as an effective way to mitigate the problem of low dissolved oxygen in the discharge of hydroelectric power plants. The design of such a hydroturbine requires a precise understanding of the dependence of the generated bubble size distribution upon the operating conditions (viz. liquid velocity, air ventilation rate, hydrofoil configuration, etc.) and the consequent rise in dissolved oxygen in the downstream water. The purpose of the current research is to investigate the effect of location of air injection on the resulting bubble size distribution, thus leading to a quantitative analysis of aeration statistics and capabilities for two turbine blade hydrofoil designs. The two blade designs differed in their location of air injection. Extensive sets of experiments were conducted by varying the liquid velocity, aeration rate and the hydrofoil angle of attack, to characterize the resulting bubble size distribution. Using a shadow imaging technique to capture the bubble images in the wake and an in-house developed image analysis algorithm, it was found that the hydrofoil with leading edge ventilation produced smaller size bubbles as compared to the hydrofoil being ventilated at the trailing edge.

Soluble Prokaryotic Expression and Purification of Bioactive Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand

  • Do, Bich Hang;Nguyen, Minh Tan;Song, Jung-A;Park, Sangsu;Yoo, Jiwon;Jang, Jaepyeong;Lee, Sunju;So, Seoungjun;Yoon, Yejin;Kim, Inki;Lee, Kyungjin;Jang, Yeon Jin;Choe, Han
    • Journal of Microbiology and Biotechnology
    • /
    • 제27권12호
    • /
    • pp.2156-2164
    • /
    • 2017
  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered as an antitumor agent owing to its ability to induce apoptosis of cancer cells without imparting toxicity toward most normal cells. TRAIL is produced in poor yield because of its insoluble expression in the cytoplasm of E. coli. In this study, we achieved soluble expression of TRAIL by fusing maltose-binding protein (MBP), b'a' domain of protein disulfide isomerase (PDIb'a'), or protein disulfide isomerase at the N-terminus of TRAIL. The TRAIL was purified using subsequent immobilized metal affinity chromatography and amylose-binding chromatography, with the tag removal using tobacco etch virus protease. Approximately 4.5 mg of pure TRAIL was produced from 125 ml flask culture with a purification yield of 71.6%. The endotoxin level of the final product was $0.4EU/{\mu}g$, as measured by the Limulus amebocyte lysate endotoxin assay. The purified TRAIL was validated and shown to cause apoptosis of HeLa cells with an $EC_{50}$ and Hill coefficient of $0.6{{\pm}}0.03nM$ and $2.41{\pm}0.15$, respectively. The high level of apoptosis in HeLa cells following administration of purified TRAIL indicates the significance and novelty of this method for producing high-grade and high-yield TRAIL.